BQ624275

Overview
NameBQ624275
Unique NameBQ624275
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length606
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of BQ624275 vs. ExPASy Swiss-Prot
Match: UBC4_YEAST (Ubiquitin-conjugating enzyme E2 4 OS=Saccharomyces cerevisiae GN=UBC4 PE=1 SV=1)

HSP 1 Score: 213.386 bits (542), Expect = 3.289e-64
Identity = 92/117 (78.63%), Postives = 110/117 (94.02%), Query Frame = 3 
Query:  156 ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDP 506
            +SKRI KEL DL++DPPTSCSAGPV +D++HWQA+IMGP+DSPYAGGVF ++IHFP DYPFKPPK++F TK++HPNIN+NG+ICLDILK+QWSPALT+SKVLLSICSLLTD NPDDP
Sbjct:    3 SSKRIAKELSDLERDPPTSCSAGPVGDDLYHWQASIMGPADSPYAGGVFFLSIHFPTDYPFKPPKISFTTKIYHPNINANGNICLDILKDQWSPALTLSKVLLSICSLLTDANPDDP 119

HSP 2 Score: 53.1434 bits (126), Expect = 3.289e-64
Identity = 21/30 (70.00%), Postives = 27/30 (90.00%), Query Frame = 1 
Query:  505 PLVPEIAHMYKSDKAKYESTGRSWTQKYAM 594
            PLVPEIAH+YK+D+ KYE+T R WT+KYA+
Sbjct:  119 PLVPEIAHIYKTDRPKYEATAREWTKKYAV 148
[Close]
BLAST of BQ624275 vs. ExPASy Swiss-Prot
Match: UBC1_COLGL (Ubiquitin-conjugating enzyme E2-16 kDa OS=Colletotrichum gloeosporioides GN=UBC1 PE=2 SV=1)

HSP 1 Score: 212.616 bits (540), Expect = 3.290e-64
Identity = 95/118 (80.51%), Postives = 107/118 (90.68%), Query Frame = 3 
Query:  153 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDP 506
            MA KRI KEL DL +DPP+SCSAGPV ED+FHWQATIMGP DSPY+GGVF + IHFP DYPFKPPKV F T+++HPNINSNGSICLDIL++QWSPALTISKVLLSICS+LTDPNPD+P
Sbjct:    1 MAFKRINKELTDLGRDPPSSCSAGPVGEDLFHWQATIMGPGDSPYSGGVFFLAIHFPTDYPFKPPKVNFTTRIYHPNINSNGSICLDILRDQWSPALTISKVLLSICSMLTDPNPDEP 118

HSP 2 Score: 53.9138 bits (128), Expect = 3.290e-64
Identity = 21/30 (70.00%), Postives = 28/30 (93.33%), Query Frame = 1 
Query:  505 PLVPEIAHMYKSDKAKYESTGRSWTQKYAM 594
            PLVPEIAH+YK+D+A+YE+T R WT+KYA+
Sbjct:  118 PLVPEIAHVYKTDRARYEATAREWTRKYAI 147
[Close]
BLAST of BQ624275 vs. ExPASy Swiss-Prot
Match: UBC4_SCHPO (Ubiquitin-conjugating enzyme E2 4 OS=Schizosaccharomyces pombe GN=ubc4 PE=2 SV=1)

HSP 1 Score: 215.698 bits (548), Expect = 5.595e-64
Identity = 97/118 (82.20%), Postives = 109/118 (92.37%), Query Frame = 3 
Query:  153 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDP 506
            MA KRI +EL DL KDPP+SCSAGPV +D+FHWQATIMGP+DSPYAGGVF ++IHFP DYPFKPPKV F T+++HPNINSNGSICLDIL++QWSPALTISKVLLSICSLLTDPNPDDP
Sbjct:    1 MALKRINRELADLGKDPPSSCSAGPVGDDLFHWQATIMGPADSPYAGGVFFLSIHFPTDYPFKPPKVNFTTRIYHPNINSNGSICLDILRDQWSPALTISKVLLSICSLLTDPNPDDP 118

HSP 2 Score: 50.0618 bits (118), Expect = 5.595e-64
Identity = 19/30 (63.33%), Postives = 27/30 (90.00%), Query Frame = 1 
Query:  505 PLVPEIAHMYKSDKAKYESTGRSWTQKYAM 594
            PLVPEIAH+YK+D+++YE + R WT+KYA+
Sbjct:  118 PLVPEIAHVYKTDRSRYELSAREWTRKYAI 147
[Close]
BLAST of BQ624275 vs. ExPASy Swiss-Prot
Match: UB2D2_XENLA (Ubiquitin-conjugating enzyme E2 D2 OS=Xenopus laevis GN=ube2d2 PE=1 SV=1)

HSP 1 Score: 214.542 bits (545), Expect = 4.676e-63
Identity = 97/118 (82.20%), Postives = 105/118 (88.98%), Query Frame = 3 
Query:  153 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDP 506
            MA KRI KEL DL +DPP  CSAGPV +DMFHWQATIMGP+DSPY GGVF +TIHFP DYPFKPPKVAF T+++HPNINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDP
Sbjct:    1 MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDP 118

HSP 2 Score: 48.1358 bits (113), Expect = 4.676e-63
Identity = 20/30 (66.67%), Postives = 23/30 (76.67%), Query Frame = 1 
Query:  505 PLVPEIAHMYKSDKAKYESTGRSWTQKYAM 594
            PLVPEIA +YK+D+ KY    R WTQKYAM
Sbjct:  118 PLVPEIARIYKTDREKYNRIAREWTQKYAM 147
[Close]
BLAST of BQ624275 vs. ExPASy Swiss-Prot
Match: UB2D2_RAT (Ubiquitin-conjugating enzyme E2 D2 OS=Rattus norvegicus GN=Ube2d2 PE=2 SV=1)

HSP 1 Score: 214.542 bits (545), Expect = 4.676e-63
Identity = 97/118 (82.20%), Postives = 105/118 (88.98%), Query Frame = 3 
Query:  153 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDP 506
            MA KRI KEL DL +DPP  CSAGPV +DMFHWQATIMGP+DSPY GGVF +TIHFP DYPFKPPKVAF T+++HPNINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDP
Sbjct:    1 MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDP 118

HSP 2 Score: 48.1358 bits (113), Expect = 4.676e-63
Identity = 20/30 (66.67%), Postives = 23/30 (76.67%), Query Frame = 1 
Query:  505 PLVPEIAHMYKSDKAKYESTGRSWTQKYAM 594
            PLVPEIA +YK+D+ KY    R WTQKYAM
Sbjct:  118 PLVPEIARIYKTDREKYNRIAREWTQKYAM 147
[Close]
BLAST of BQ624275 vs. ExPASy Swiss-Prot
Match: UB2D2_MOUSE (Ubiquitin-conjugating enzyme E2 D2 OS=Mus musculus GN=Ube2d2 PE=1 SV=1)

HSP 1 Score: 214.542 bits (545), Expect = 4.676e-63
Identity = 97/118 (82.20%), Postives = 105/118 (88.98%), Query Frame = 3 
Query:  153 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDP 506
            MA KRI KEL DL +DPP  CSAGPV +DMFHWQATIMGP+DSPY GGVF +TIHFP DYPFKPPKVAF T+++HPNINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDP
Sbjct:    1 MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDP 118

HSP 2 Score: 48.1358 bits (113), Expect = 4.676e-63
Identity = 20/30 (66.67%), Postives = 23/30 (76.67%), Query Frame = 1 
Query:  505 PLVPEIAHMYKSDKAKYESTGRSWTQKYAM 594
            PLVPEIA +YK+D+ KY    R WTQKYAM
Sbjct:  118 PLVPEIARIYKTDREKYNRIAREWTQKYAM 147
[Close]
BLAST of BQ624275 vs. ExPASy Swiss-Prot
Match: UB2D2_HUMAN (Ubiquitin-conjugating enzyme E2 D2 OS=Homo sapiens GN=UBE2D2 PE=1 SV=1)

HSP 1 Score: 214.542 bits (545), Expect = 4.676e-63
Identity = 97/118 (82.20%), Postives = 105/118 (88.98%), Query Frame = 3 
Query:  153 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDP 506
            MA KRI KEL DL +DPP  CSAGPV +DMFHWQATIMGP+DSPY GGVF +TIHFP DYPFKPPKVAF T+++HPNINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDP
Sbjct:    1 MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDP 118

HSP 2 Score: 48.1358 bits (113), Expect = 4.676e-63
Identity = 20/30 (66.67%), Postives = 23/30 (76.67%), Query Frame = 1 
Query:  505 PLVPEIAHMYKSDKAKYESTGRSWTQKYAM 594
            PLVPEIA +YK+D+ KY    R WTQKYAM
Sbjct:  118 PLVPEIARIYKTDREKYNRIAREWTQKYAM 147
[Close]
BLAST of BQ624275 vs. ExPASy Swiss-Prot
Match: UB2D2_BOVIN (Ubiquitin-conjugating enzyme E2 D2 OS=Bos taurus GN=UBE2D2 PE=2 SV=1)

HSP 1 Score: 214.542 bits (545), Expect = 4.676e-63
Identity = 97/118 (82.20%), Postives = 105/118 (88.98%), Query Frame = 3 
Query:  153 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDP 506
            MA KRI KEL DL +DPP  CSAGPV +DMFHWQATIMGP+DSPY GGVF +TIHFP DYPFKPPKVAF T+++HPNINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDP
Sbjct:    1 MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDP 118

HSP 2 Score: 48.1358 bits (113), Expect = 4.676e-63
Identity = 20/30 (66.67%), Postives = 23/30 (76.67%), Query Frame = 1 
Query:  505 PLVPEIAHMYKSDKAKYESTGRSWTQKYAM 594
            PLVPEIA +YK+D+ KY    R WTQKYAM
Sbjct:  118 PLVPEIARIYKTDREKYNRIAREWTQKYAM 147
[Close]
BLAST of BQ624275 vs. ExPASy Swiss-Prot
Match: UB2D3_RAT (Ubiquitin-conjugating enzyme E2 D3 OS=Rattus norvegicus GN=Ube2d3 PE=2 SV=1)

HSP 1 Score: 214.542 bits (545), Expect = 6.098e-63
Identity = 97/118 (82.20%), Postives = 105/118 (88.98%), Query Frame = 3 
Query:  153 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDP 506
            MA KRI KEL DL +DPP  CSAGPV +DMFHWQATIMGP+DSPY GGVF +TIHFP DYPFKPPKVAF T+++HPNINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDP
Sbjct:    1 MALKRINKELSDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDP 118

HSP 2 Score: 47.7506 bits (112), Expect = 6.098e-63
Identity = 20/30 (66.67%), Postives = 23/30 (76.67%), Query Frame = 1 
Query:  505 PLVPEIAHMYKSDKAKYESTGRSWTQKYAM 594
            PLVPEIA +YK+D+ KY    R WTQKYAM
Sbjct:  118 PLVPEIARIYKTDRDKYNRISREWTQKYAM 147
[Close]
BLAST of BQ624275 vs. ExPASy Swiss-Prot
Match: UB2D3_PONAB (Ubiquitin-conjugating enzyme E2 D3 OS=Pongo abelii GN=UBE2D3 PE=2 SV=1)

HSP 1 Score: 214.542 bits (545), Expect = 6.098e-63
Identity = 97/118 (82.20%), Postives = 105/118 (88.98%), Query Frame = 3 
Query:  153 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDP 506
            MA KRI KEL DL +DPP  CSAGPV +DMFHWQATIMGP+DSPY GGVF +TIHFP DYPFKPPKVAF T+++HPNINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDP
Sbjct:    1 MALKRINKELSDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDP 118

HSP 2 Score: 47.7506 bits (112), Expect = 6.098e-63
Identity = 20/30 (66.67%), Postives = 23/30 (76.67%), Query Frame = 1 
Query:  505 PLVPEIAHMYKSDKAKYESTGRSWTQKYAM 594
            PLVPEIA +YK+D+ KY    R WTQKYAM
Sbjct:  118 PLVPEIARIYKTDRDKYNRISREWTQKYAM 147
The following BLAST results are available for this feature:
BLAST of BQ624275 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 266
Match NameE-valueIdentityDescription
UBC4_YEAST3.289e-6478.63Ubiquitin-conjugating enzyme E2 4 OS=Saccharomyces... [more]
UBC1_COLGL3.290e-6480.51Ubiquitin-conjugating enzyme E2-16 kDa OS=Colletot... [more]
UBC4_SCHPO5.595e-6482.20Ubiquitin-conjugating enzyme E2 4 OS=Schizosacchar... [more]
UB2D2_XENLA4.676e-6382.20Ubiquitin-conjugating enzyme E2 D2 OS=Xenopus laev... [more]
UB2D2_RAT4.676e-6382.20Ubiquitin-conjugating enzyme E2 D2 OS=Rattus norve... [more]
UB2D2_MOUSE4.676e-6382.20Ubiquitin-conjugating enzyme E2 D2 OS=Mus musculus... [more]
UB2D2_HUMAN4.676e-6382.20Ubiquitin-conjugating enzyme E2 D2 OS=Homo sapiens... [more]
UB2D2_BOVIN4.676e-6382.20Ubiquitin-conjugating enzyme E2 D2 OS=Bos taurus G... [more]
UB2D3_RAT6.098e-6382.20Ubiquitin-conjugating enzyme E2 D3 OS=Rattus norve... [more]
UB2D3_PONAB6.098e-6382.20Ubiquitin-conjugating enzyme E2 D3 OS=Pongo abelii... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_01366 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_01366 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ624275 ID=BQ624275; Name=BQ624275; organism=Citrus sinensis; type=EST; length=606bp
GTTGATAGAGGAGCGAGGCGGGGGCGGCTTGACGACCTAGATCTGCTTCA
CCGTTTCGCTTTCTCGACGCTATCGCTCTCCGATCCTGTTCTCTGCCGCG
CAAGGTCTTAAGGTTCGTTGTAGTTTCAATTTTAATAATCCCCTGAAGAG
TCATGGCTTCAAAGCGTATTTTGAAGGAACTCAAGGATTTGCAGAAGGAT
CCTCCCACGTCTTGCAGCGCTGGCCCTGTGGCTGAAGACATGTTCCACTG
GCAAGCAACAATTATGGGCCCTTCTGATAGCCCATATGCAGGAGGTGTAT
TTTTAGTTACCATTCATTTTCCTCCTGATTATCCTTTCAAGCCCCCAAAG
GTTGCTTTTAGGACCAAGGTCTTCCACCCGAACATCAACAGCAATGGAAG
CATCTGTCTTGATATCCTTAAAGAACAGTGGAGTCCAGCGCTTACCATTT
CAAAGGTCCTGCTCTCTATCTGCTCCTTGCTTACTGATCCAAATCCTGAC
GACCCCTCTTGTACCGGAGATTGCCCACATGTACAAGTCCGACAAGGCCA
AATATGAATCCACGGGCAGGAGCTGGACACAGAAGTATGCCATGGGATGA
AGGTTG
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