CB250245

Overview
NameCB250245
Unique NameCB250245
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length709
Libraries
Library NameType
Mature fruit abscission zone cDNA subtraction librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig13846 contig Csv1_Contig13846:683..1391. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HSP7A_CAEEL (Heat shock 70 kDa protein A OS=Caenorhabditis elegans GN=hsp-1 PE=1 SV=2)

HSP 1 Score: 334.339 bits (856), Expect = 3.766e-91
Identity = 171/235 (72.77%), Postives = 187/235 (79.57%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            RMVNHF  EFKRK+KKD++ NPRALRRLRTACERAKRTLSS++Q +IEIDSL+EGIDFYT ITRARFEEL  DLFR  M+PVEK LRDAKMDKS VHD+VLVGGSTRIPKVQ+LL D F+GKEL KSINPDE           ILSG+ +E VQD             ETAGGVMT LI RNTTIPTK  Q F+TYSDNQPGVLIQVYEGER  T+DNNLLGKF+ SGIPPAPRG
Sbjct:  237 RMVNHFCAEFKRKHKKDLASNPRALRRLRTACERAKRTLSSSSQASIEIDSLFEGIDFYTNITRARFEELCADLFRSTMDPVEKSLRDAKMDKSQVHDIVLVGGSTRIPKVQKLLSDLFSGKELNKSINPDEAVAYGAAVQAAILSGDKSEAVQDLLLLDVAPLSLGIETAGGVMTALIKRNTTIPTKTAQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRG 471
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HS71L_PIG (Heat shock 70 kDa protein 1-like OS=Sus scrofa GN=HSPA1L PE=2 SV=1)

HSP 1 Score: 334.339 bits (856), Expect = 3.766e-91
Identity = 168/235 (71.49%), Postives = 191/235 (81.28%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R+V+HFV+EFKRK+KKDIS N RA+RRLRTACERAKRTLSS+ Q  +EIDSLYEGIDFYT+ITRARFEEL  DLFR  +EPVEK LRDAKMDK+ +HD+VLVGGSTRIPKVQ+LLQD+FNG++L KSINPDE           IL G+ +EKVQD             ETAGGVMTVLI RN+TIPTK  Q+F+TYSDNQPGVLIQVYEGER  TRDNNLLG+FD +GIPPAPRG
Sbjct:  238 RLVSHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQANLEIDSLYEGIDFYTSITRARFEELCADLFRGTLEPVEKALRDAKMDKAKIHDIVLVGGSTRIPKVQRLLQDYFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSEKVQDLLLLDVAPLSLGLETAGGVMTVLIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTRDNNLLGRFDLTGIPPAPRG 472
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HS71L_BOVIN (Heat shock 70 kDa protein 1-like OS=Bos taurus GN=HSPA1L PE=3 SV=1)

HSP 1 Score: 334.339 bits (856), Expect = 3.766e-91
Identity = 168/235 (71.49%), Postives = 191/235 (81.28%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R+V+HFV+EFKRK+KKDIS N RA+RRLRTACERAKRTLSS+ Q  +EIDSLYEGIDFYT+ITRARFEEL  DLFR  +EPVEK LRDAKMDK+ +HD+VLVGGSTRIPKVQ+LLQD+FNG++L KSINPDE           IL G+ +EKVQD             ETAGGVMTVLI RN+TIPTK  Q+F+TYSDNQPGVLIQVYEGER  TRDNNLLG+FD +GIPPAPRG
Sbjct:  238 RLVSHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQANLEIDSLYEGIDFYTSITRARFEELCADLFRGTLEPVEKALRDAKMDKAKIHDIVLVGGSTRIPKVQRLLQDYFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSEKVQDLLLLDVAPLSLGLETAGGVMTVLIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTRDNNLLGRFDLTGIPPAPRG 472
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HS71L_RAT (Heat shock 70 kDa protein 1-like OS=Rattus norvegicus GN=Hspa1l PE=2 SV=2)

HSP 1 Score: 333.954 bits (855), Expect = 4.919e-91
Identity = 168/235 (71.49%), Postives = 191/235 (81.28%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R+V+HFV+EFKRK+KKDIS N RA+RRLRTACERAKRTLSS+ Q  +EIDSLYEGIDFYT+ITRARFEEL  DLFR  +EPVEK LRDAKMDK+ +HD+VLVGGSTRIPKVQ+LLQD+FNG++L KSINPDE           IL G+ +EKVQD             ETAGGVMTVLI RN+TIPTK  Q+F+TYSDNQPGVLIQVYEGER  TRDNNLLG+FD +GIPPAPRG
Sbjct:  238 RLVSHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQANLEIDSLYEGIDFYTSITRARFEELCADLFRGTLEPVEKSLRDAKMDKAKIHDIVLVGGSTRIPKVQKLLQDYFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSEKVQDLLLLDVAPLSLGLETAGGVMTVLIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTRDNNLLGRFDLTGIPPAPRG 472
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HS71L_MOUSE (Heat shock 70 kDa protein 1-like OS=Mus musculus GN=Hspa1l PE=2 SV=4)

HSP 1 Score: 333.954 bits (855), Expect = 4.919e-91
Identity = 168/235 (71.49%), Postives = 191/235 (81.28%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R+V+HFV+EFKRK+KKDIS N RA+RRLRTACERAKRTLSS+ Q  +EIDSLYEGIDFYT+ITRARFEEL  DLFR  +EPVEK LRDAKMDK+ +HD+VLVGGSTRIPKVQ+LLQD+FNG++L KSINPDE           IL G+ +EKVQD             ETAGGVMTVLI RN+TIPTK  Q+F+TYSDNQPGVLIQVYEGER  TRDNNLLG+FD +GIPPAPRG
Sbjct:  238 RLVSHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQANLEIDSLYEGIDFYTSITRARFEELCADLFRGTLEPVEKSLRDAKMDKAKIHDIVLVGGSTRIPKVQKLLQDYFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSEKVQDLLLLDVAPLSLGLETAGGVMTVLIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTRDNNLLGRFDLTGIPPAPRG 472
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HSP72_USTMA (Heat shock 70 kDa protein 2 OS=Ustilago maydis GN=UMS2 PE=3 SV=2)

HSP 1 Score: 333.569 bits (854), Expect = 6.424e-91
Identity = 169/235 (71.91%), Postives = 190/235 (80.85%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R+VNHFVQEFKRKNKKD++ N RALRRLRTACERAKRTLSS AQTTIEIDSL+EGIDFYT+ITRARFEEL  DLF   +EPVEK LRD+K+DK SVH++VLVGGSTRIPKVQ+LL DFFNG+EL KSINPDE           ILSG+ +EK QD             ETAGGV T LI RNTT+PTK  ++FSTY+DNQPGVLIQV+EGER RT+DNNLLGKF+ SGIPPAPRG
Sbjct:  234 RLVNHFVQEFKRKNKKDLTTNARALRRLRTACERAKRTLSSAAQTTIEIDSLFEGIDFYTSITRARFEELCGDLFSHTIEPVEKVLRDSKIDKGSVHEIVLVGGSTRIPKVQKLLTDFFNGRELNKSINPDEAVAYGAAVQAAILSGDTSEKTQDLLLLDVAPLSMGIETAGGVFTPLIKRNTTVPTKKSEIFSTYADNQPGVLIQVFEGERARTKDNNLLGKFELSGIPPAPRG 468
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HSP70_ONCTS (Heat shock 70 kDa protein OS=Oncorhynchus tschawytscha GN=hsp70 PE=2 SV=1)

HSP 1 Score: 333.569 bits (854), Expect = 6.424e-91
Identity = 169/235 (71.91%), Postives = 191/235 (81.28%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R+V+HFV+EFKRK+KKDIS N RALRRLRTACERAKRTLSS++Q +IEIDSL+EGIDFYT+ITRARFEE+  DLFR  +EPVEK LRDAKMDK+ +HDVVLVGGSTRIPKVQ+LLQDFFNG+EL KSINPDE           ILSG+ +E VQD             ETAGGVMT LI RNTTIP+K  Q F+TYSDNQPGV+IQVYEGER  T+DNNLLGKF+ SGIPPAPRG
Sbjct:  238 RLVSHFVEEFKRKHKKDISQNKRALRRLRTACERAKRTLSSSSQASIEIDSLFEGIDFYTSITRARFEEMCSDLFRGTLEPVEKALRDAKMDKAQIHDVVLVGGSTRIPKVQKLLQDFFNGRELNKSINPDEAVAYGAAIQAAILSGDKSENVQDLLLLDVAPLSLGIETAGGVMTALIKRNTTIPSKQTQTFTTYSDNQPGVMIQVYEGERAMTKDNNLLGKFELSGIPPAPRG 472
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HSP7D_DROME (Heat shock 70 kDa protein cognate 4 OS=Drosophila melanogaster GN=Hsc70-4 PE=1 SV=3)

HSP 1 Score: 332.413 bits (851), Expect = 1.431e-90
Identity = 168/235 (71.49%), Postives = 189/235 (80.43%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R+V HFVQEFKRK+KKD++ N RALRRLRTACERAKRTLSS+ Q +IEIDSL+EG DFYT+ITRARFEELN DLFR  M+PVEK LRDAK+DKS +HD+VLVGGSTRIPKVQ+LLQD FNGKEL KSINPDE           IL G+ +++VQD             ETAGGVM+VLI RNTTIPTK  Q F+TYSDNQPGVLIQVYEGER  T+DNNLLGKF+ SGIPPAPRG
Sbjct:  236 RLVTHFVQEFKRKHKKDLTTNKRALRRLRTACERAKRTLSSSTQASIEIDSLFEGTDFYTSITRARFEELNADLFRSTMDPVEKALRDAKLDKSVIHDIVLVGGSTRIPKVQRLLQDLFNGKELNKSINPDEAVAYGAAVQAAILHGDKSQEVQDLLLLDVTPLSLGIETAGGVMSVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRG 470
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HSP70_XENLA (Heat shock 70 kDa protein OS=Xenopus laevis PE=2 SV=1)

HSP 1 Score: 332.028 bits (850), Expect = 1.869e-90
Identity = 169/235 (71.91%), Postives = 189/235 (80.43%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            RMVNHFV+EFKRK+KKDI  N RALRRLRTAC+RAKRTLSS++Q +IEIDSL+EGIDFYT ITRARFEEL  DLFR  +EPVEK LRDAK+DKS +H++VLVGGSTRIPKVQ+LLQDFFNG+EL KSINPDE           IL G+ +E VQD             ETAGGVMTVLI RNTTIPTK  Q F+TYSDNQPGVLIQV+EGER  T+DNNLLGKF+ SGIPPAPRG
Sbjct:  237 RMVNHFVEEFKRKHKKDIGQNKRALRRLRTACDRAKRTLSSSSQASIEIDSLFEGIDFYTAITRARFEELCSDLFRGTLEPVEKALRDAKLDKSQIHEIVLVGGSTRIPKVQKLLQDFFNGRELNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTVLIKRNTTIPTKQTQSFTTYSDNQPGVLIQVFEGERAMTKDNNLLGKFELSGIPPAPRG 471
[Close]
BLAST of CB250245 vs. ExPASy Swiss-Prot
Match: HS71L_MACFA (Heat shock 70 kDa protein 1-like OS=Macaca fascicularis GN=HSPA1L PE=2 SV=1)

HSP 1 Score: 331.643 bits (849), Expect = 2.441e-90
Identity = 166/235 (70.64%), Postives = 190/235 (80.85%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKNEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFDPSGIPPAPRG 707
            R+V+HFV+EFKRK+KKDIS N RA+RRLRTACERAKRTLSS+ Q  +EIDSLYEGIDFYT+ITRARFEEL  DLFR  +EPVEK LRDAKMDK+ +HD+VLVGGSTRIPKVQ+LLQD+FNG++L KSINPDE           IL G+ +EKVQD             ETAGGVMT LI RN+TIPTK  Q+F+TYSDNQPGVLIQVYEGER  T+DNNLLG+FD +GIPPAPRG
Sbjct:  238 RLVSHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQANLEIDSLYEGIDFYTSITRARFEELCADLFRGTLEPVEKALRDAKMDKAKIHDIVLVGGSTRIPKVQRLLQDYFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSEKVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTKDNNLLGRFDLTGIPPAPRG 472
The following BLAST results are available for this feature:
BLAST of CB250245 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
HSP7A_CAEEL3.766e-9172.77Heat shock 70 kDa protein A OS=Caenorhabditis eleg... [more]
HS71L_PIG3.766e-9171.49Heat shock 70 kDa protein 1-like OS=Sus scrofa GN=... [more]
HS71L_BOVIN3.766e-9171.49Heat shock 70 kDa protein 1-like OS=Bos taurus GN=... [more]
HS71L_RAT4.919e-9171.49Heat shock 70 kDa protein 1-like OS=Rattus norvegi... [more]
HS71L_MOUSE4.919e-9171.49Heat shock 70 kDa protein 1-like OS=Mus musculus G... [more]
HSP72_USTMA6.424e-9171.91Heat shock 70 kDa protein 2 OS=Ustilago maydis GN=... [more]
HSP70_ONCTS6.424e-9171.91Heat shock 70 kDa protein OS=Oncorhynchus tschawyt... [more]
HSP7D_DROME1.431e-9071.49Heat shock 70 kDa protein cognate 4 OS=Drosophila ... [more]
HSP70_XENLA1.869e-9071.91Heat shock 70 kDa protein OS=Xenopus laevis PE=2 S... [more]
HS71L_MACFA2.441e-9070.64Heat shock 70 kDa protein 1-like OS=Macaca fascicu... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionEST0007 Mature fruit abscission zone cDNA subtraction library Citrus sinensis cDNA clone MFAZ007 similar to 70 kDa heat shock protein (acc# P27322), mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CB250245 ID=CB250245; Name=CB250245; organism=Citrus sinensis; type=EST; length=709bp
ACAGAATGGTGAACCACTTTGTTCAAGAGTTCAAGAGGAAAAACAAGAAG
GATATTAGTGGAAACCCCAGAGCGTTGAGGAGGTTGAGAACCGCCTGTGA
AAGGGCTAAGAGGACCCTGTCATCAACTGCACAGACTACAATTGAGATTG
ATTCTTTATATGAGGGTATTGATTTCTACACAACCATTACAAGGGCTAGA
TTCGAAGAGTTGAACATGGATTTGTTCAGGAAGTGTATGGAGCCAGTGGA
GAAATGCCTGAGAGATGCTAAGATGGACAAGAGCAGTGTCCATGATGTGG
TTCTTGTTGGTGGGTCTACTAGGATCCCAAAAGTCCAGCAACTGTTGCAG
GACTTCTTCAATGGAAAAGAGCTATGCAAGAGCATCAACCCTGATGAAGC
GGTTGCCTACGGTGCTGCTGTGCAGGCTGCAATCTTGAGTGGTGAGGGTA
ATGAGAAGGTTCAGGACCTCTTATTGCTTGACGTCACCCCTCTATCGCTG
GGTTTGGAGACTGCTGGTGGAGTCATGACTGTGTTGATTCCAAGGAACAC
CACTATTCCAACCAAGAATGAGCAGGTGTTCTCCACATACTCTGACAACC
AGCCCGGGGTGTTGATCCAGGTCTACGAGGGTGAGCGCACAAGAACCCGT
GACAACAACTTGCTTGGAAAGTTTGATCCCTCTGGCATTCCTCCTGCTCC
AAGAGGGGT
back to top