CB250343

Overview
NameCB250343
Unique NameCB250343
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length481
Libraries
Library NameType
Mature fruit abscission zone cDNA subtraction librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig2108 contig Csv1_Contig2108:261..741. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CB250343 vs. ExPASy Swiss-Prot
Match: WRBA_ECO7I (Flavoprotein wrbA OS=Escherichia coli O7:K1 (strain IAI39 / ExPEC) GN=wrbA PE=3 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 4.694e-30
Identity = 69/142 (48.59%), Postives = 89/142 (62.68%), Query Frame = 2 
Query:    2 PIITPNELAEADGFVFGFPTRFGTMAAQFKAFLDATGGLWRTQQLAGKPAGMFYXXXXXXXXXXXXALTAITQLVHHGMIFVPIGYTFGAGMFEMEKVKGGSPYGAGTFA-GDGSRQPTELELEQAFHQGKHTAGIAKKLKG 424
            P+ TP ELA+ D  +FG PTRFG M+ Q + FLD TGGLW +  L GK A +F               T  T L HHGM+ VPIGY     +F++ +V+GG+PYGA T A GDGSRQP++ EL  A +QG++ AG+A KL G
Sbjct:   59 PVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGALYGKLASVFSSTGTGGGQEQTITST-WTTLAHHGMVIVPIGYA-AQELFDVSQVRGGTPYGATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG 198
[Close]
BLAST of CB250343 vs. ExPASy Swiss-Prot
Match: WRBA_ECO55 (Flavoprotein wrbA OS=Escherichia coli (strain 55989 / EAEC) GN=wrbA PE=3 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 4.694e-30
Identity = 69/142 (48.59%), Postives = 89/142 (62.68%), Query Frame = 2 
Query:    2 PIITPNELAEADGFVFGFPTRFGTMAAQFKAFLDATGGLWRTQQLAGKPAGMFYXXXXXXXXXXXXALTAITQLVHHGMIFVPIGYTFGAGMFEMEKVKGGSPYGAGTFA-GDGSRQPTELELEQAFHQGKHTAGIAKKLKG 424
            P+ TP ELA+ D  +FG PTRFG M+ Q + FLD TGGLW +  L GK A +F               T  T L HHGM+ VPIGY     +F++ +V+GG+PYGA T A GDGSRQP++ EL  A +QG++ AG+A KL G
Sbjct:   59 PVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGALYGKLASVFSSTGTGGGQEQTITST-WTTLAHHGMVIVPIGYA-AQELFDVSQVRGGTPYGATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG 198
[Close]
BLAST of CB250343 vs. ExPASy Swiss-Prot
Match: WRBA_ECO45 (Flavoprotein wrbA OS=Escherichia coli O45:K1 (strain S88 / ExPEC) GN=wrbA PE=3 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 4.694e-30
Identity = 69/142 (48.59%), Postives = 89/142 (62.68%), Query Frame = 2 
Query:    2 PIITPNELAEADGFVFGFPTRFGTMAAQFKAFLDATGGLWRTQQLAGKPAGMFYXXXXXXXXXXXXALTAITQLVHHGMIFVPIGYTFGAGMFEMEKVKGGSPYGAGTFA-GDGSRQPTELELEQAFHQGKHTAGIAKKLKG 424
            P+ TP ELA+ D  +FG PTRFG M+ Q + FLD TGGLW +  L GK A +F               T  T L HHGM+ VPIGY     +F++ +V+GG+PYGA T A GDGSRQP++ EL  A +QG++ AG+A KL G
Sbjct:   59 PVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGALYGKLASVFSSTGTGGGQEQTITST-WTTLAHHGMVIVPIGYA-AQELFDVSQVRGGTPYGATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG 198
[Close]
BLAST of CB250343 vs. ExPASy Swiss-Prot
Match: WRBA_ECO27 (Flavoprotein wrbA OS=Escherichia coli O127:H6 (strain E2348/69 / EPEC) GN=wrbA PE=3 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 4.694e-30
Identity = 69/142 (48.59%), Postives = 89/142 (62.68%), Query Frame = 2 
Query:    2 PIITPNELAEADGFVFGFPTRFGTMAAQFKAFLDATGGLWRTQQLAGKPAGMFYXXXXXXXXXXXXALTAITQLVHHGMIFVPIGYTFGAGMFEMEKVKGGSPYGAGTFA-GDGSRQPTELELEQAFHQGKHTAGIAKKLKG 424
            P+ TP ELA+ D  +FG PTRFG M+ Q + FLD TGGLW +  L GK A +F               T  T L HHGM+ VPIGY     +F++ +V+GG+PYGA T A GDGSRQP++ EL  A +QG++ AG+A KL G
Sbjct:   59 PVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGALYGKLASVFSSTGTGGGQEQTITST-WTTLAHHGMVIVPIGYA-AQELFDVSQVRGGTPYGATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG 198
[Close]
BLAST of CB250343 vs. ExPASy Swiss-Prot
Match: WRBA_ECO24 (Flavoprotein wrbA OS=Escherichia coli O139:H28 (strain E24377A / ETEC) GN=wrbA PE=3 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 4.694e-30
Identity = 69/142 (48.59%), Postives = 89/142 (62.68%), Query Frame = 2 
Query:    2 PIITPNELAEADGFVFGFPTRFGTMAAQFKAFLDATGGLWRTQQLAGKPAGMFYXXXXXXXXXXXXALTAITQLVHHGMIFVPIGYTFGAGMFEMEKVKGGSPYGAGTFA-GDGSRQPTELELEQAFHQGKHTAGIAKKLKG 424
            P+ TP ELA+ D  +FG PTRFG M+ Q + FLD TGGLW +  L GK A +F               T  T L HHGM+ VPIGY     +F++ +V+GG+PYGA T A GDGSRQP++ EL  A +QG++ AG+A KL G
Sbjct:   59 PVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGALYGKLASVFSSTGTGGGQEQTITST-WTTLAHHGMVIVPIGYA-AQELFDVSQVRGGTPYGATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG 198
[Close]
BLAST of CB250343 vs. ExPASy Swiss-Prot
Match: WRBA_METEP (Flavoprotein wrbA OS=Methylobacterium extorquens (strain PA1) GN=wrbA PE=3 SV=1)

HSP 1 Score: 129.413 bits (324), Expect = 8.007e-30
Identity = 71/142 (50.00%), Postives = 86/142 (60.56%), Query Frame = 2 
Query:    2 PIITPNELAEADGFVFGFPTRFGTMAAQFKAFLDATGGLWRTQQLAGKPAGMFYXXXXXXXXXXXXALTAITQLVHHGMIFVPIGYTFGAGMFEMEKVKGGSPYGAGTFA-GDGSRQPTELELEQAFHQGKHTAGIAKKLKG 424
            PI T  ELA+ D  +FG PTR+G MA+Q K F+D TGGLW    L GK    F               +  T L HHGM+ V + Y+F AG   +E+VKG SPYGA T A GDGSRQP+E+EL+ A  QG+H AGIA KL G
Sbjct:   59 PIATVEELADYDAIIFGTPTRYGNMASQMKQFIDQTGGLWAKGALVGKVGSAFTSTASQHGGQETTLTSFHTVLFHHGMVVVGLPYSF-AGQNGVEQVKGNSPYGATTIADGDGSRQPSEVELDGARFQGRHVAGIAAKLAG 199
[Close]
BLAST of CB250343 vs. ExPASy Swiss-Prot
Match: WRBA_METC4 (Flavoprotein wrbA OS=Methylobacterium chloromethanicum (strain CM4 / NCIMB 13688) GN=wrbA PE=3 SV=1)

HSP 1 Score: 129.413 bits (324), Expect = 8.007e-30
Identity = 71/142 (50.00%), Postives = 86/142 (60.56%), Query Frame = 2 
Query:    2 PIITPNELAEADGFVFGFPTRFGTMAAQFKAFLDATGGLWRTQQLAGKPAGMFYXXXXXXXXXXXXALTAITQLVHHGMIFVPIGYTFGAGMFEMEKVKGGSPYGAGTFA-GDGSRQPTELELEQAFHQGKHTAGIAKKLKG 424
            PI T  ELA+ D  +FG PTR+G MA+Q K F+D TGGLW    L GK    F               +  T L HHGM+ V + Y+F AG   +E+VKG SPYGA T A GDGSRQP+E+EL+ A  QG+H AGIA KL G
Sbjct:   59 PIATVEELADYDAIIFGTPTRYGNMASQMKQFIDQTGGLWAKGALVGKVGSAFTSTASQHGGQETTLTSFHTVLFHHGMVVVGLPYSF-AGQNGVEQVKGNSPYGATTIADGDGSRQPSEVELDGARFQGRHVAGIAAKLAG 199
[Close]
BLAST of CB250343 vs. ExPASy Swiss-Prot
Match: WRBA_KLEP3 (Flavoprotein wrbA OS=Klebsiella pneumoniae (strain 342) GN=wrbA PE=3 SV=1)

HSP 1 Score: 129.413 bits (324), Expect = 8.007e-30
Identity = 71/142 (50.00%), Postives = 88/142 (61.97%), Query Frame = 2 
Query:    2 PIITPNELAEADGFVFGFPTRFGTMAAQFKAFLDATGGLWRTQQLAGKPAGMFYXXXXXXXXXXXXALTAITQLVHHGMIFVPIGYTFGAGMFEMEKVKGGSPYGAGTFA-GDGSRQPTELELEQAFHQGKHTAGIAKKLKG 424
            P+ TP ELAE D  +FG PTRFG M+ Q + FLD TGGLW +  L GK A +F               T  T L HHGMI VPIGY     +F++ +V+GG+PYGA T A GDGSRQP+E EL  A +QG+H A +A K+ G
Sbjct:   59 PVATPQELAEYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGALYGKIASVFSSTGTGGGQEQTITST-WTTLAHHGMIIVPIGYG-AQELFDISQVRGGTPYGATTIAGGDGSRQPSEEELAIARYQGEHVAKLAVKVHG 198
[Close]
BLAST of CB250343 vs. ExPASy Swiss-Prot
Match: WRBA_ECO5E (Flavoprotein wrbA OS=Escherichia coli O157:H7 (strain EC4115 / EHEC) GN=wrbA PE=3 SV=1)

HSP 1 Score: 129.413 bits (324), Expect = 8.007e-30
Identity = 69/142 (48.59%), Postives = 88/142 (61.97%), Query Frame = 2 
Query:    2 PIITPNELAEADGFVFGFPTRFGTMAAQFKAFLDATGGLWRTQQLAGKPAGMFYXXXXXXXXXXXXALTAITQLVHHGMIFVPIGYTFGAGMFEMEKVKGGSPYGAGTFA-GDGSRQPTELELEQAFHQGKHTAGIAKKLKG 424
            P+ TP ELA  D  +FG PTRFG M+ Q + FLD TGGLW +  L GK A +F               T  T L HHGM+ VPIGY     +F++ +V+GG+PYGA T A GDGSRQP++ EL  A +QG++ AG+A KL G
Sbjct:   59 PVATPQELANYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGALYGKLASVFSSTGTGGGQEQTITST-WTTLAHHGMVIVPIGYA-AQELFDVSQVRGGTPYGATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG 198
[Close]
BLAST of CB250343 vs. ExPASy Swiss-Prot
Match: WRBA_ECO57 (Putative flavoprotein wrbA OS=Escherichia coli O157:H7 GN=wrbA PE=5 SV=3)

HSP 1 Score: 129.413 bits (324), Expect = 8.007e-30
Identity = 69/142 (48.59%), Postives = 88/142 (61.97%), Query Frame = 2 
Query:    2 PIITPNELAEADGFVFGFPTRFGTMAAQFKAFLDATGGLWRTQQLAGKPAGMFYXXXXXXXXXXXXALTAITQLVHHGMIFVPIGYTFGAGMFEMEKVKGGSPYGAGTFA-GDGSRQPTELELEQAFHQGKHTAGIAKKLKG 424
            P+ TP ELA  D  +FG PTRFG M+ Q + FLD TGGLW +  L GK A +F               T  T L HHGM+ VPIGY     +F++ +V+GG+PYGA T A GDGSRQP++ EL  A +QG++ AG+A KL G
Sbjct:   59 PVATPQELANYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGALYGKLASVFSSTGTGGGQEQTITST-WTTLAHHGMVIVPIGYA-AQELFDVSQVRGGTPYGATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG 198
The following BLAST results are available for this feature:
BLAST of CB250343 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 146
Match NameE-valueIdentityDescription
WRBA_ECO7I4.694e-3048.59Flavoprotein wrbA OS=Escherichia coli O7:K1 (strai... [more]
WRBA_ECO554.694e-3048.59Flavoprotein wrbA OS=Escherichia coli (strain 5598... [more]
WRBA_ECO454.694e-3048.59Flavoprotein wrbA OS=Escherichia coli O45:K1 (stra... [more]
WRBA_ECO274.694e-3048.59Flavoprotein wrbA OS=Escherichia coli O127:H6 (str... [more]
WRBA_ECO244.694e-3048.59Flavoprotein wrbA OS=Escherichia coli O139:H28 (st... [more]
WRBA_METEP8.007e-3050.00Flavoprotein wrbA OS=Methylobacterium extorquens (... [more]
WRBA_METC48.007e-3050.00Flavoprotein wrbA OS=Methylobacterium chloromethan... [more]
WRBA_KLEP38.007e-3050.00Flavoprotein wrbA OS=Klebsiella pneumoniae (strain... [more]
WRBA_ECO5E8.007e-3048.59Flavoprotein wrbA OS=Escherichia coli O157:H7 (str... [more]
WRBA_ECO578.007e-3048.59Putative flavoprotein wrbA OS=Escherichia coli O15... [more]

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Properties
Property NameValue
Genbank descriptionEST0133 Mature fruit abscission zone cDNA subtraction library Citrus sinensis cDNA clone MFAZ197 similar to 1,4-benzoquinone reductase (acc# NP_194457), mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CB250343 ID=CB250343; Name=CB250343; organism=Citrus sinensis; type=EST; length=481bp
ACCTATTATTACCCCCAACGAACTTGCTGAGGCTGATGGGTTTGTTTTTG
GCTTCCCAACAAGATTTGGAACGATGGCTGCTCAATTTAAAGCATTTTTA
GATGCTACTGGTGGTCTATGGAGGACACAACAGCTCGCTGGCAAACCTGC
TGGGATGTTCTACAGCACTGGGTCACAAGGTGGTGGACAAGAGACTACAG
CGTTGACGGCAATTACTCAGCTTGTTCACCATGGGATGATATTTGTTCCT
ATTGGATACACATTTGGTGCTGGCATGTTTGAGATGGAGAAAGTGAAAGG
TGGAAGTCCCTACGGTGCAGGAACTTTTGCTGGAGACGGCTCGAGACAGC
CAACTGAGCTTGAGTTGGAGCAAGCTTTCCACCAGGGAAAACACACTGCG
GGCATTGCAAAGAAGCTGAAGGGAAGTGCTTAAATATTTCAATTTACAAT
ACAACCATTAGGTGGACTTATTCCATTCTTT
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