CB304892

Overview
NameCB304892
Unique NameCB304892
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length618
Libraries
Library NameType
Flavedo Maturecdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CB304892 vs. ExPASy Swiss-Prot
Match: RL14_METB6 (50S ribosomal protein L14P OS=Methanoregula boonei (strain 6A8) GN=rpl14p PE=3 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 9.777e-23
Identity = 51/112 (45.54%), Postives = 75/112 (66.96%), Query Frame = -3
Query:  224 LPVAATVNCADNTGAKNLYIISVKGIKGRLNRLPSACVGDMVMATVKKGKPDLRKKVMPAVIVRQRKPWRRKDGVFMYFEDNAGVIVNPKGEMKGSAIPGPIGKECADLWPR 559
            L     + CADNTGA+ + I+SV G  G   R P   +GD+   TVKKG PD+RKK++ AV++RQ+K  RR +G+ + F+DNA V+V+ K E KG+ I GP+ +E A+ +P+
Sbjct:   12 LATGTKLTCADNTGARIVQIVSVFGYHGVRRRQPKMGLGDIATVTVKKGTPDMRKKLVRAVVIRQKKEMRRPNGLRVSFDDNAVVVVDEKNEPKGTEIKGPVAREVAERYPK 123          
BLAST of CB304892 vs. ExPASy Swiss-Prot
Match: RL14_METLZ (50S ribosomal protein L14P OS=Methanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z) GN=rpl14p PE=3 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 4.852e-22
Identity = 48/112 (42.86%), Postives = 78/112 (69.64%), Query Frame = -3
Query:  224 LPVAATVNCADNTGAKNLYIISVKGIKGRLNRLPSACVGDMVMATVKKGKPDLRKKVMPAVIVRQRKPWRRKDGVFMYFEDNAGVIVNPKGEMKGSAIPGPIGKECADLWPR 559
            L   + + CADNTGA+ + I+SV G  G  NR P   +GD+   TVKKG PD+++K++ AV+VRQ+K +RR +G+ + FE+NA +++N  G+ +G+ I GP+ +E A+ +P+
Sbjct:   12 LQTGSKMVCADNTGARVVQIVSVFGYHGVKNRQPKMGLGDLATVTVKKGTPDMKRKLVRAVVVRQKKEFRRPNGLRVSFEENAMILLNENGDPRGTDIKGPVAREVAERFPK 123          
BLAST of CB304892 vs. ExPASy Swiss-Prot
Match: RL14_UNCMA (50S ribosomal protein L14P OS=Uncultured methanogenic archaeon RC-I GN=rpl14p PE=3 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 4.108e-21
Identity = 47/119 (39.50%), Postives = 79/119 (66.39%), Query Frame = -3
Query:  224 KFRMSLGLPVAATVNCADNTGAKNLYIISVKGIKGRLNRLPSACVGDMVMATVKKGKPDLRKKVMPAVIVRQRKPWRRKDGVFMYFEDNAGVIVNPKGEMKGSAIPGPIGKECADLWPR 580
            K ++   +  A  +  ADNTGA+ + +ISV   +GR NR+P A +GD+ + +VKKG P++RK ++  V++RQ+K +RR  G+ + FEDNA V+V+   + KG+ I GP+ +E A+ + +
Sbjct:    5 KAKIPRSVHTATRLEVADNTGARLVEVISVLKYRGRKNRMPKAGIGDIFIGSVKKGTPEMRKTIVRCVVIRQKKEYRRPSGLRVSFEDNACVLVDDNNDPKGTEIKGPVAREVAERYAK 123          
BLAST of CB304892 vs. ExPASy Swiss-Prot
Match: RL14_METHJ (50S ribosomal protein L14P OS=Methanospirillum hungatei (strain JF-1 / DSM 864) GN=rpl14p PE=3 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 2.039e-20
Identity = 47/106 (44.34%), Postives = 71/106 (66.98%), Query Frame = -3
Query:  224 VNCADNTGAKNLYIISVKGIKGRLNRLPSACVGDMVMATVKKGKPDLRKKVMPAVIVRQRKPWRRKDGVFMYFEDNAGVIVNPKGEMKGSAIPGPIGKECADLWPR 541
            + CADNTGA+ + ++SV G  G   R P   + D+   +VKKG PD+R+K++ AV++RQ+K  RR  G+ + FEDNA V+V+ K E +G+ I GP+ +E A  +PR
Sbjct:   18 LTCADNTGARVVQVVSVFGYHGVRRRQPKLGLADIATVSVKKGTPDMRRKLVRAVVIRQKKEIRRPSGLRLSFEDNAVVVVDDKNEPRGTEIKGPVAREVAIRYPR 123          
BLAST of CB304892 vs. ExPASy Swiss-Prot
Match: RL14_METMJ (50S ribosomal protein L14P OS=Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1) GN=rpl14p PE=3 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 5.932e-20
Identity = 46/112 (41.07%), Postives = 74/112 (66.07%), Query Frame = -3
Query:  224 LPVAATVNCADNTGAKNLYIISVKGIKGRLNRLPSACVGDMVMATVKKGKPDLRKKVMPAVIVRQRKPWRRKDGVFMYFEDNAGVIVNPKGEMKGSAIPGPIGKECADLWPR 559
            L   + + C+DNTGA+ + ++SV    G   R P   +GD+   +VKKG PD+R+K+  AV++RQ+K  RR +G+ + FEDNA V++N +GE KG+ I G + +E A+ +P+
Sbjct:   12 LATGSRMVCSDNTGARLVEVVSVDRYHGVRRRQPCLGLGDVATVSVKKGTPDMRRKLEKAVVIRQKKEIRRPNGIRLSFEDNAMVLINERGEPKGTEIKGAVPREIAERFPK 123          
BLAST of CB304892 vs. ExPASy Swiss-Prot
Match: RL14_DEHE1 (50S ribosomal protein L14 OS=Dehalococcoides ethenogenes (strain 195) GN=rplN PE=3 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 1.047e-16
Identity = 49/105 (46.67%), Postives = 67/105 (63.81%), Query Frame = -3
Query:  236 VNCADNTGAKNLYIISVKGIKGRLNRLPSACVGDMVMATVKKGKPDLRKK---VMPAVIVRQRKPWRRKDGVFMYFEDNAGVIVNPKGEMKGSAIPGPIGKECAD 541
            +N ADNTGAK +  I+V G   +L     A VGD+++A VKK  PD + K   V+ AV+VR  KP+ R DG ++ F+DNA VI+N K E KG+ I GP+ +E  D
Sbjct:    8 LNVADNTGAKKIMCINVLGGSRKLQ----AKVGDVIVAAVKKSSPDAQAKSGTVVKAVVVRITKPYARPDGSYIKFDDNAAVILNDKMEPKGTRIFGPVARELRD 108          
BLAST of CB304892 vs. ExPASy Swiss-Prot
Match: RL14_DEHSC (50S ribosomal protein L14 OS=Dehalococcoides sp. (strain CBDB1) GN=rplN PE=3 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 1.786e-16
Identity = 48/105 (45.71%), Postives = 67/105 (63.81%), Query Frame = -3
Query:  236 VNCADNTGAKNLYIISVKGIKGRLNRLPSACVGDMVMATVKKGKPDLRKK---VMPAVIVRQRKPWRRKDGVFMYFEDNAGVIVNPKGEMKGSAIPGPIGKECAD 541
            +N ADNTGAK +  I+V G   ++     A VGD+++A VKK  PD + K   V+ AV+VR  KP+ R DG ++ F+DNA VI+N K E KG+ I GP+ +E  D
Sbjct:    8 LNVADNTGAKKIMCINVLGGSHKIQ----AKVGDVIVAAVKKSSPDAQAKSGTVVKAVVVRITKPYARPDGSYIKFDDNAAVILNDKMEPKGTRIFGPVARELRD 108          
BLAST of CB304892 vs. ExPASy Swiss-Prot
Match: RL14_DEHSB (50S ribosomal protein L14 OS=Dehalococcoides sp. (strain BAV1) GN=rplN PE=3 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 1.786e-16
Identity = 48/105 (45.71%), Postives = 67/105 (63.81%), Query Frame = -3
Query:  236 VNCADNTGAKNLYIISVKGIKGRLNRLPSACVGDMVMATVKKGKPDLRKK---VMPAVIVRQRKPWRRKDGVFMYFEDNAGVIVNPKGEMKGSAIPGPIGKECAD 541
            +N ADNTGAK +  I+V G   ++     A VGD+++A VKK  PD + K   V+ AV+VR  KP+ R DG ++ F+DNA VI+N K E KG+ I GP+ +E  D
Sbjct:    8 LNVADNTGAKKIMCINVLGGSHKIQ----AKVGDVIVAAVKKSSPDAQAKSGTVVKAVVVRITKPYARPDGSYIKFDDNAAVILNDKMEPKGTRIFGPVARELRD 108          
BLAST of CB304892 vs. ExPASy Swiss-Prot
Match: RL14_THERP (50S ribosomal protein L14 OS=Thermomicrobium roseum (strain ATCC 27502 / DSM 5159 / P-2) GN=rplN PE=3 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 2.579e-15
Identity = 46/99 (46.46%), Postives = 68/99 (68.69%), Query Frame = -3
Query:  245 ADNTGAKNLYIISVKGIKGRLNRLPSACVGDMVMATVKKGKPDLRKK---VMPAVIVRQRKPWRRKDGVFMYFEDNAGVIVNPKGEMKGSAIPGPIGKE 532
            ADNTGAK +  I V G  G  +R  +  VGD+++A+VKK +P+   K   V+ AVIVR +K +RRKDG ++ F+DNA V+V P+G+ +G+ I GP+ +E
Sbjct:   11 ADNTGAKEIMCIRVLG--GSKHRYGT--VGDLIVASVKKAEPNAAVKKGDVVYAVIVRTKKEYRRKDGSYIKFDDNAAVLVTPQGQPRGTRIFGPVARE 105          
BLAST of CB304892 vs. ExPASy Swiss-Prot
Match: RL14_SACEN (50S ribosomal protein L14 OS=Saccharopolyspora erythraea (strain NRRL 23338) GN=rplN PE=3 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 3.368e-15
Identity = 46/102 (45.10%), Postives = 64/102 (62.75%), Query Frame = -3
Query:  236 ADNTGAKNLYIISVKGIKGRLNRLPSACVGDMVMATVKKGKPDLRKK---VMPAVIVRQRKPWRRKDGVFMYFEDNAGVIVNPKGEMKGSAIPGPIGKECAD 532
            ADN+GAK +  I V G  GR      A +GD+++ATVK   P    K   V+ AVIVRQ+K  RR DG ++ F++NA V++ P GE +G+ I GP+G+E  D
Sbjct:   11 ADNSGAKEILTIRVLGGSGRRY----AGIGDIIVATVKDAIPGANVKKGEVVKAVIVRQKKEKRRADGSYIRFDENAAVLIKPSGEPRGTRIFGPVGRELRD 108          
The following BLAST results are available for this feature:
BLAST of CB304892 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 356
Match NameE-valueIdentityDescription
RL14_METB69.777e-2345.5450S ribosomal protein L14P OS=Methanoregula boonei... [more]
RL14_METLZ4.852e-2242.8650S ribosomal protein L14P OS=Methanocorpusculum l... [more]
RL14_UNCMA4.108e-2139.5050S ribosomal protein L14P OS=Uncultured methanoge... [more]
RL14_METHJ2.039e-2044.3450S ribosomal protein L14P OS=Methanospirillum hun... [more]
RL14_METMJ5.932e-2041.0750S ribosomal protein L14P OS=Methanoculleus maris... [more]
RL14_DEHE11.047e-1646.6750S ribosomal protein L14 OS=Dehalococcoides ethen... [more]
RL14_DEHSC1.786e-1645.7150S ribosomal protein L14 OS=Dehalococcoides sp. (... [more]
RL14_DEHSB1.786e-1645.7150S ribosomal protein L14 OS=Dehalococcoides sp. (... [more]
RL14_THERP2.579e-1546.4650S ribosomal protein L14 OS=Thermomicrobium roseu... [more]
RL14_SACEN3.368e-1545.1050S ribosomal protein L14 OS=Saccharopolyspora ery... [more]

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Properties
Property NameValue
Genbank descriptionFLAVEDO0002_IaR_F07 Flavedo Mature Citrus sinensis cDNA clone FLAVEDO0002_IaR_F07 3, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CB304892 ID=CB304892; Name=CB304892; organism=Citrus sinensis; type=EST; length=618bp
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAGATCAACAAAACTCCAAA
CTTTCATACCACTTACAAAACTAGAATAACAAAATCATACAGAAAGTTCA
CAGAAAAGCTTTACTCCTTCCTACTATGAAACTTCACCAGATTTCTTTTT
AGTGCAACAAAAAGGATGATATAGGTTATCGGTCTTGAATGGCTTAAACG
ATAGCATTGGCTGCACTTGCAATTCTCGGCCACAAATCAGCACACTCTTT
ACCAATTGGACCGGGGATGGCAGAACCCTTCATTTCTCCCTTTGGATTCA
CAATAACACCAGCATTATCTTCAAAGTACATGAAGACACCATCCTTTCTG
CGCCATGGCTTGCGCTGCCTTACAATGACGGCGGGCATCACCTTCTTCCT
AAGATCAGGCTTCCCTTTCTTCACGGTAGCCATAACCATGTCTCCAACAC
AGGCAGACGGCAAACGGTTGAGGCGACCTTTGATTCCCTTAACTGAGATG
ATGTAAAGGTTCTTTGCCCCAGTGTTGTCAGCACAGTTCACAGTTGCTGC
CACTGGCAAACCCAGTGACATCCTGAACTTGTTACCTGCTGATCCTCCAC
GTCCTCGCTTCGACATTT
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