CB305058

Overview
NameCB305058
Unique NameCB305058
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length643
Libraries
Library NameType
Flavedo Maturecdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CB305058 vs. ExPASy Swiss-Prot
Match: HSP16_SOYBN (18.5 kDa class I heat shock protein OS=Glycine max GN=HSP18.5-C PE=3 SV=1)

HSP 1 Score: 149.443 bits (376), Expect = 1.428e-35
Identity = 74/120 (61.67%), Postives = 88/120 (73.33%), Query Frame = -3
Query:  270 PSSARETSQFANARIDWKETPQAHVFKADLPGLRXXXXXXXXXEGRILQISGERSKEQEEKNDKWHRVERSSGKFLRRFRLPDNAKVEQVKASMENGVLTXXXXXXXXXXXXXXAIEISG 629
            P  +RE S F + R+DWKETP+AHVFKAD+PGL+         + ++LQISGER+ E+E+KND WHRVERSSGKF+RRFRLP+NAKVEQVKASMENGVLT              AIEISG
Sbjct:   42 PEFSRENSAFVSTRVDWKETPEAHVFKADIPGLKKEEVKVQIEDDKVLQISGERNVEKEDKNDTWHRVERSSGKFMRRFRLPENAKVEQVKASMENGVLTVTVPKEEVKKPDVKAIEISG 161          
BLAST of CB305058 vs. ExPASy Swiss-Prot
Match: HS177_ORYSJ (17.7 kDa class I heat shock protein OS=Oryza sativa subsp. japonica GN=HSP17.7 PE=2 SV=1)

HSP 1 Score: 149.058 bits (375), Expect = 1.866e-35
Identity = 74/120 (61.67%), Postives = 87/120 (72.50%), Query Frame = -3
Query:  270 PSSARETSQFANARIDWKETPQAHVFKADLPGLRXXXXXXXXXEGRILQISGERSKEQEEKNDKWHRVERSSGKFLRRFRLPDNAKVEQVKASMENGVLTXXXXXXXXXXXXXXAIEISG 629
            P +  + + FA ARIDWKETP+ HVFKAD+PGL+         +G ILQISGERS+EQEEK+DKWHRVERSSGKFLRRFRLP+N K EQ+KASMENGVLT              +I+ISG
Sbjct:   40 PRANSDAAAFAGARIDWKETPEVHVFKADVPGLKKEEVKVEVDDGNILQISGERSREQEEKSDKWHRVERSSGKFLRRFRLPENTKPEQIKASMENGVLTVTVPKEEPKKPDVKSIQISG 159          
BLAST of CB305058 vs. ExPASy Swiss-Prot
Match: HS16A_ORYSJ (16.9 kDa class I heat shock protein 1 OS=Oryza sativa subsp. japonica GN=HSP16.9A PE=1 SV=1)

HSP 1 Score: 148.673 bits (374), Expect = 2.437e-35
Identity = 74/119 (62.18%), Postives = 90/119 (75.63%), Query Frame = -3
Query:  270 SSARETSQFANARIDWKETPQAHVFKADLPGLRXXXXXXXXXEGRILQISGERSKEQEEKNDKWHRVERSSGKFLRRFRLPDNAKVEQVKASMENGVLTXXXXXXXXXXXXXXAIEISG 626
            +S  +T+ FANARIDWKETP++HVFKADLPG++         EG +L ISG+RSKE+E+KNDKWHRVERSSG+F+RRFRLP+NAKV+QVKA +ENGVLT              AIEISG
Sbjct:   32 TSDNDTAAFANARIDWKETPESHVFKADLPGVKKEEVKVEVEEGNVLVISGQRSKEKEDKNDKWHRVERSSGQFMRRFRLPENAKVDQVKAGLENGVLTVTVPKAEVKKPEVKAIEISG 150          
BLAST of CB305058 vs. ExPASy Swiss-Prot
Match: HSP12_DAUCA (18.0 kDa class I heat shock protein OS=Daucus carota PE=3 SV=1)

HSP 1 Score: 147.517 bits (371), Expect = 5.428e-35
Identity = 74/116 (63.79%), Postives = 86/116 (74.14%), Query Frame = -3
Query:  270 RETSQFANARIDWKETPQAHVFKADLPGLRXXXXXXXXXEGRILQISGERSKEQEEKNDKWHRVERSSGKFLRRFRLPDNAKVEQVKASMENGVLTXXXXXXXXXXXXXXAIEISG 617
            +ET+ FAN  IDWKETPQAHVFKADLPGL+         EG++LQISGER+KE+EEKN+KWHRVE SSGKFLRRFRLP+NA V++VKA MENGVLT              +I ISG
Sbjct:   44 KETAAFANTHIDWKETPQAHVFKADLPGLKKEEVKVEVEEGKVLQISGERNKEKEEKNNKWHRVEFSSGKFLRRFRLPENANVDEVKAGMENGVLTVTVPKVEMKKPEVKSIHISG 159          
BLAST of CB305058 vs. ExPASy Swiss-Prot
Match: HS174_ORYSJ (17.4 kDa class I heat shock protein OS=Oryza sativa subsp. japonica GN=HSP17.4 PE=2 SV=2)

HSP 1 Score: 147.517 bits (371), Expect = 5.428e-35
Identity = 73/120 (60.83%), Postives = 86/120 (71.67%), Query Frame = -3
Query:  270 PSSARETSQFANARIDWKETPQAHVFKADLPGLRXXXXXXXXXEGRILQISGERSKEQEEKNDKWHRVERSSGKFLRRFRLPDNAKVEQVKASMENGVLTXXXXXXXXXXXXXXAIEISG 629
            P +  + + FA ARIDWKETP+AHVFKAD+PGL+         +G +LQISGER KEQEEK DKWHRVERSSGKFLRRFRLP+N K EQ+KASMENGVLT              +I+I+G
Sbjct:   35 PRANSDAAAFAGARIDWKETPEAHVFKADVPGLKKEEVKVEVEDGNVLQISGERIKEQEEKTDKWHRVERSSGKFLRRFRLPENTKPEQIKASMENGVLTVTVPKEEPKKPDVKSIQITG 154          
BLAST of CB305058 vs. ExPASy Swiss-Prot
Match: HSP11_SOLLC (17.8 kDa class I heat shock protein OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 147.132 bits (370), Expect = 7.089e-35
Identity = 73/119 (61.34%), Postives = 89/119 (74.79%), Query Frame = -3
Query:  270 SSARETSQFANARIDWKETPQAHVFKADLPGLRXXXXXXXXXEGRILQISGERSKEQEEKNDKWHRVERSSGKFLRRFRLPDNAKVEQVKASMENGVLTXXXXXXXXXXXXXXAIEISG 626
            +++ E+S FAN RIDWKETP+ HVFK DLPGL+         E R+LQISGER+ E+E+KNDKWHR+ERSSGKF+RRFRLP+NAK++QVKASMENGVLT              +IEISG
Sbjct:   36 TNSGESSAFANTRIDWKETPEPHVFKVDLPGLKKEEVKVEVEEDRVLQISGERNVEKEDKNDKWHRMERSSGKFMRRFRLPENAKMDQVKASMENGVLTVTVPKEEVKKPEVKSIEISG 154          
BLAST of CB305058 vs. ExPASy Swiss-Prot
Match: HSP11_PEA (18.1 kDa class I heat shock protein OS=Pisum sativum GN=HSP18.1 PE=2 SV=1)

HSP 1 Score: 146.747 bits (369), Expect = 9.259e-35
Identity = 74/124 (59.68%), Postives = 92/124 (74.19%), Query Frame = -3
Query:  270 NAPSSA--RETSQFANARIDWKETPQAHVFKADLPGLRXXXXXXXXXEGRILQISGERSKEQEEKNDKWHRVERSSGKFLRRFRLPDNAKVEQVKASMENGVLTXXXXXXXXXXXXXXAIEISG 635
            ++PS++  RE   F + R+DWKETP+AHVFKADLPGL+         + R+LQISGERS E+E+KND+WHRVERSSGKFLRRFRLP+NAK+++VKASMENGVLT              +IEISG
Sbjct:   35 SSPSASFPRENPAFVSTRVDWKETPEAHVFKADLPGLKKEEVKVEVEDDRVLQISGERSVEKEDKNDEWHRVERSSGKFLRRFRLPENAKMDKVKASMENGVLTVTVPKEEIKKAEVKSIEISG 158          
BLAST of CB305058 vs. ExPASy Swiss-Prot
Match: HS16A_WHEAT (16.9 kDa class I heat shock protein 1 OS=Triticum aestivum GN=hsp16.9A PE=2 SV=1)

HSP 1 Score: 146.747 bits (369), Expect = 9.259e-35
Identity = 71/115 (61.74%), Postives = 87/115 (75.65%), Query Frame = -3
Query:  270 ETSQFANARIDWKETPQAHVFKADLPGLRXXXXXXXXXEGRILQISGERSKEQEEKNDKWHRVERSSGKFLRRFRLPDNAKVEQVKASMENGVLTXXXXXXXXXXXXXXAIEISG 614
            ET+ FANAR+DWKETP+AHVFK DLPG++         +G +L +SGERS+E+E+KNDKWHRVERSSGKF+RRFRLP++AKVE+VKA +ENGVLT              AIEISG
Sbjct:   37 ETAAFANARVDWKETPEAHVFKVDLPGVKKEEVKVEVEDGNVLVVSGERSREKEDKNDKWHRVERSSGKFVRRFRLPEDAKVEEVKAGLENGVLTVTVPKAEVKKPEVKAIEISG 151          
BLAST of CB305058 vs. ExPASy Swiss-Prot
Match: HSP11_CHERU (18.3 kDa class I heat shock protein OS=Chenopodium rubrum GN=HSP18 PE=2 SV=1)

HSP 1 Score: 146.362 bits (368), Expect = 1.209e-34
Identity = 70/106 (66.04%), Postives = 87/106 (82.08%), Query Frame = -3
Query:  330 LTNAPSS--ARETSQFANARIDWKETPQAHVFKADLPGLRXXXXXXXXXEGRILQISGERSKEQEEKNDKWHRVERSSGKFLRRFRLPDNAKVEQVKASMENGVLT 641
            L+  P S  A ET+ FANARIDWKETP+AHVFKADLPG++         +G +L+ISG+R++E+EEKND WHRVERSSG+F+R+FRLP+NAKV+QVKA MENGVLT
Sbjct:   37 LSTVPRSETAAETAAFANARIDWKETPEAHVFKADLPGVKKEEVKVEVEDGNVLRISGQRAREKEEKNDTWHRVERSSGQFMRKFRLPENAKVDQVKAGMENGVLT 142          
BLAST of CB305058 vs. ExPASy Swiss-Prot
Match: HSP13_SOYBN (17.5 kDa class I heat shock protein OS=Glycine max GN=HSP17.5-M PE=3 SV=1)

HSP 1 Score: 145.591 bits (366), Expect = 2.063e-34
Identity = 73/119 (61.34%), Postives = 86/119 (72.27%), Query Frame = -3
Query:  270 SSARETSQFANARIDWKETPQAHVFKADLPGLRXXXXXXXXXEGRILQISGERSKEQEEKNDKWHRVERSSGKFLRRFRLPDNAKVEQVKASMENGVLTXXXXXXXXXXXXXXAIEISG 626
            S + E S F N R+DWKETP+AHVF+AD+PGL+         + R+LQISGER+ E+E+KND WHRVERSSG F+RRFRLP+NAKVEQVKASMENGVLT              AIEISG
Sbjct:   35 SLSAENSAFVNTRVDWKETPEAHVFEADIPGLKKEEVKVQIEDDRVLQISGERNLEKEDKNDTWHRVERSSGNFMRRFRLPENAKVEQVKASMENGVLTVTVPKEEVKKPDVKAIEISG 153          
The following BLAST results are available for this feature:
BLAST of CB305058 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 54
Match NameE-valueIdentityDescription
HSP16_SOYBN1.428e-3561.6718.5 kDa class I heat shock protein OS=Glycine max... [more]
HS177_ORYSJ1.866e-3561.6717.7 kDa class I heat shock protein OS=Oryza sativ... [more]
HS16A_ORYSJ2.437e-3562.1816.9 kDa class I heat shock protein 1 OS=Oryza sat... [more]
HSP12_DAUCA5.428e-3563.7918.0 kDa class I heat shock protein OS=Daucus caro... [more]
HS174_ORYSJ5.428e-3560.8317.4 kDa class I heat shock protein OS=Oryza sativ... [more]
HSP11_SOLLC7.089e-3561.3417.8 kDa class I heat shock protein OS=Solanum lyc... [more]
HSP11_PEA9.259e-3559.6818.1 kDa class I heat shock protein OS=Pisum sativ... [more]
HS16A_WHEAT9.259e-3561.7416.9 kDa class I heat shock protein 1 OS=Triticum ... [more]
HSP11_CHERU1.209e-3466.0418.3 kDa class I heat shock protein OS=Chenopodium... [more]
HSP13_SOYBN2.063e-3461.3417.5 kDa class I heat shock protein OS=Glycine max... [more]

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Properties
Property NameValue
Genbank descriptionFLAVEDO0003_IVaR_E03 Flavedo Mature Citrus sinensis cDNA clone FLAVEDO0003_IVaR_E03 3, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CB305058 ID=CB305058; Name=CB305058; organism=Citrus sinensis; type=EST; length=643bp
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTGCATTACAAAATATATT
TTATTGCATCACACTTTTATCATTTAAAAAACTAGTTACAACGCTTGAGA
CTGGCTCCTGAAGCTCTTGCAAACACACAAACATAATCACATCAAATTAA
ACAGGGGGGAAGGGAAGCTCTTGCAAACACACACACACACACACAAGGGC
TTTGCTTTCGTACCAAATTTATTAGCAAACCAAAGACACAATCTTGAAAA
CATTTACACTTATCACTTAGCCAGAGATCTCAATGGCCTTAACTTCAGGT
TTCTTCTCCTCCTGTTTAGGAACAGCCACTGTGAGCACACCATTCTCCAT
GCTGGCCTTAACCTGCTCCACCTTAGCATTGTCGGGCAACCTGAACCTCC
TCAGGAACTTGCCACTGCTCCTCTCGACGCGATGCCACTTGTCATTCTTC
TCTTCCTGCTCCTTGCTCCTCTCCCCGCTTATCTGCAGAATCCTGCCCTC
TTCAACCTCAACTTTCACTTCTTCTTTCCTCAGCCCTGGAAGATCAGCCT
TGAATACATGAGCCTGAGGAGTCTCTTTCCAGTCAATTCTTGCATTGGCG
AATTGGGATGTTTCCCTAGCCGAAGACGGCGCGTTGGTAAGGG
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