CB322149 | Citrus Genome Database

Overview


Name	CB322149
Unique Name	CB322149
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	469

Libraries


Library Name	Type
Mature leaf blade cDNA subtraction library	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CB322149 vs. ExPASy Swiss-Prot
Match: HSP72_BOVIN (Heat shock-related 70 kDa protein 2 OS=Bos taurus GN=HSPA2 PE=2 SV=2)

HSP 1 Score: 242.276 bits (617), Expect = 7.856e-64
Identity = 120/155 (77.42%), Postives = 134/155 (86.45%), Query Frame = 3

Query:    3 SCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFSDASVQGDMKLWPFKVIAGPADKPMIGVNYKGEEKQFAAEEISSMVLIKMREIAEAYLGATIKNAVVTVPAYFNDSQRQATKDAGVIAGLNV 467
            SCVGV+QH +VEIIANDQGNRTTPSYV FTDTERLIGDAAKNQVAMNPTNT+FDAKRLIGR+F DA+VQ DMK WPF+V++    KP + V YKGE K F  EEISSMVL KM+EIAEAYLG  +++AV+TVPAYFNDSQRQATKDAG I GLNV
Sbjct:   17 SCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTIFDAKRLIGRKFEDATVQSDMKHWPFRVVS-EGGKPKVQVEYKGEIKTFFPEEISSMVLTKMKEIAEAYLGGKVQSAVITVPAYFNDSQRQATKDAGTITGLNV 170

[Close]

BLAST of CB322149 vs. ExPASy Swiss-Prot
Match: HSP70_PLAFA (Heat shock 70 kDa protein OS=Plasmodium falciparum PE=2 SV=2)

HSP 1 Score: 242.276 bits (617), Expect = 7.856e-64
Identity = 120/155 (77.42%), Postives = 136/155 (87.74%), Query Frame = 3

Query:    3 SCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFSDASVQGDMKLWPFKVIAGPADKPMIGVNYKGEEKQFAAEEISSMVLIKMREIAEAYLGATIKNAVVTVPAYFNDSQRQATKDAGVIAGLNV 467
            SCVGVW+++ V+IIANDQGNRTTPSYV FTDTERLIGDAAKNQVA NP NTVFDAKRLIGR+F+++SVQ DMK WPF V +G  +KPMI V Y+GE+K F  EEISSMVL KM+E AEA+LG +IKNAV+TVPAYFNDSQRQATKDAG IAGLNV
Sbjct:   27 SCVGVWRNENVDIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVARNPENTVFDAKRLIGRKFTESSVQSDMKHWPFTVKSGVDEKPMIEVTYQGEKKLFHPEEISSMVLQKMKENAEAFLGKSIKNAVITVPAYFNDSQRQATKDAGTIAGLNV 181

[Close]

BLAST of CB322149 vs. ExPASy Swiss-Prot
Match: HSP7D_DROME (Heat shock 70 kDa protein cognate 4 OS=Drosophila melanogaster GN=Hsc70-4 PE=1 SV=3)

HSP 1 Score: 241.891 bits (616), Expect = 1.026e-63
Identity = 121/155 (78.06%), Postives = 133/155 (85.81%), Query Frame = 3

Query:    3 SCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFSDASVQGDMKLWPFKVIAGPADKPMIGVNYKGEEKQFAAEEISSMVLIKMREIAEAYLGATIKNAVVTVPAYFNDSQRQATKDAGVIAGLNV 467
            SCVGV+QH +VEIIANDQGNRTTPSYV FTDTERLIGDAAKNQVAMNPT T+FDAKRLIGR+F DA+VQ DMK WPF+V++    KP I V YK E+K F  EEISSMVL KM+E AEAYLG T+ NAV+TVPAYFNDSQRQATKDAG IAGLNV
Sbjct:   16 SCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTQTIFDAKRLIGRKFDDAAVQSDMKHWPFEVVSADG-KPKIEVTYKDEKKTFFPEEISSMVLTKMKETAEAYLGKTVTNAVITVPAYFNDSQRQATKDAGTIAGLNV 169

[Close]

BLAST of CB322149 vs. ExPASy Swiss-Prot
Match: HSP7A_DROME (Heat shock 70 kDa protein cognate 1 OS=Drosophila melanogaster GN=Hsc70-1 PE=1 SV=1)

HSP 1 Score: 241.506 bits (615), Expect = 1.340e-63
Identity = 122/155 (78.71%), Postives = 132/155 (85.16%), Query Frame = 3

Query:    3 SCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFSDASVQGDMKLWPFKVIAGPADKPMIGVNYKGEEKQFAAEEISSMVLIKMREIAEAYLGATIKNAVVTVPAYFNDSQRQATKDAGVIAGLNV 467
            SCVGV+QH +VEIIANDQGNRTTPSYV FT++ERLIGDAAKNQVAMNP NT+FDAKRLIGRRF DA+VQ DMK WPF+V A    KP I V YKGE K F  EE+SSMVL KMRE AEAYLG T+ +AVVTVPAYFNDSQRQATKDAG IAGLNV
Sbjct:   16 SCVGVFQHGKVEIIANDQGNRTTPSYVAFTESERLIGDAAKNQVAMNPNNTIFDAKRLIGRRFDDATVQSDMKHWPFEVFA-ENGKPRIRVEYKGERKSFYPEEVSSMVLTKMRETAEAYLGGTVTDAVVTVPAYFNDSQRQATKDAGAIAGLNV 169

[Close]

BLAST of CB322149 vs. ExPASy Swiss-Prot
Match: HSP70_BRUMA (Heat shock 70 kDa protein OS=Brugia malayi GN=HSP70 PE=3 SV=1)

HSP 1 Score: 241.121 bits (614), Expect = 1.750e-63
Identity = 118/155 (76.13%), Postives = 130/155 (83.87%), Query Frame = 3

Query:    3 SCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFSDASVQGDMKLWPFKVIAGPADKPMIGVNYKGEEKQFAAEEISSMVLIKMREIAEAYLGATIKNAVVTVPAYFNDSQRQATKDAGVIAGLNV 467
            SCVGV+ H +VEIIANDQGNRTTPSYV FTDTERLIGDAAKNQVAMNP NTVFDA RLIGR+F D SVQ DMK WPFKV+     KP + V YKGE K F  EEISSMVL+KM+E AEA+LG  +K+AV+TVPAYFNDSQRQATKD+G IAGLNV
Sbjct:   15 SCVGVFMHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPHNTVFDANRLIGRKFDDGSVQSDMKHWPFKVVNAGGGKPKVQVEYKGETKTFTPEEISSMVLVKMKETAEAFLGHAVKDAVITVPAYFNDSQRQATKDSGAIAGLNV 169

[Close]

BLAST of CB322149 vs. ExPASy Swiss-Prot
Match: HSP72_MOUSE (Heat shock-related 70 kDa protein 2 OS=Mus musculus GN=Hspa2 PE=1 SV=1)

HSP 1 Score: 240.736 bits (613), Expect = 2.286e-63
Identity = 119/155 (76.77%), Postives = 133/155 (85.81%), Query Frame = 3

Query:    3 SCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFSDASVQGDMKLWPFKVIAGPADKPMIGVNYKGEEKQFAAEEISSMVLIKMREIAEAYLGATIKNAVVTVPAYFNDSQRQATKDAGVIAGLNV 467
            SCVGV+QH +VEIIANDQGNRTTPSYV FTDTERLIGDAAKNQVAMNPTNT+FD KRLIGR+F DA+VQ DMK WPF+V++    KP + V YKGE K F  EEISSMVL KM+EIAEAYLG  +++AV+TVPAYFNDSQRQATKDAG I GLNV
Sbjct:   17 SCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTIFDRKRLIGRKFEDATVQSDMKHWPFRVVS-EGGKPKVQVEYKGEMKTFFPEEISSMVLTKMKEIAEAYLGGKVQSAVITVPAYFNDSQRQATKDAGTITGLNV 170

[Close]

BLAST of CB322149 vs. ExPASy Swiss-Prot
Match: HS71L_MACFA (Heat shock 70 kDa protein 1-like OS=Macaca fascicularis GN=HSPA1L PE=2 SV=1)

HSP 1 Score: 240.736 bits (613), Expect = 2.286e-63
Identity = 122/155 (78.71%), Postives = 133/155 (85.81%), Query Frame = 3

Query:    3 SCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFSDASVQGDMKLWPFKVIAGPADKPMIGVNYKGEEKQFAAEEISSMVLIKMREIAEAYLGATIKNAVVTVPAYFNDSQRQATKDAGVIAGLNV 467
            SCVGV+QH +VEIIANDQGNRTTPSYV FTDTERLIGDAAKNQVAMNP NTVFDAKRLIGR+F+D  VQ DMKLWPF+VI     KP + V+YKGE K F  EEISSMVL KM+E AEA+LG  + NAV+TVPAYFNDSQRQATKDAGVIAGLNV
Sbjct:   18 SCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPQNTVFDAKRLIGRKFNDPVVQSDMKLWPFQVI-NEGGKPKVLVSYKGENKAFYPEEISSMVLTKMKETAEAFLGHPVTNAVITVPAYFNDSQRQATKDAGVIAGLNV 171

[Close]

BLAST of CB322149 vs. ExPASy Swiss-Prot
Match: HS71A_PIG (Heat shock 70 kDa protein 1A OS=Sus scrofa GN=HSPA1A PE=3 SV=1)

HSP 1 Score: 240.736 bits (613), Expect = 2.286e-63
Identity = 122/155 (78.71%), Postives = 132/155 (85.16%), Query Frame = 3

Query:    3 SCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFSDASVQGDMKLWPFKVIAGPADKPMIGVNYKGEEKQFAAEEISSMVLIKMREIAEAYLGATIKNAVVTVPAYFNDSQRQATKDAGVIAGLNV 467
            SCVGV+QH +VEIIANDQGNRTTPS V FTDTERLIGDAAKNQVA+NP NTVFDAKRLIGR+F D  VQGDMK WPF+VI    DKP + V+YKGE K F  EEISSMVL KM+EIAE YLG  + NAV+TVPAYFNDSQRQATKDAGVIAGLNV
Sbjct:   16 SCVGVFQHGKVEIIANDQGNRTTPSSVAFTDTERLIGDAAKNQVALNPQNTVFDAKRLIGRKFGDPVVQGDMKHWPFRVI-NDGDKPKVQVSYKGETKGFYPEEISSMVLTKMKEIAEGYLGHPVSNAVITVPAYFNDSQRQATKDAGVIAGLNV 169

[Close]

BLAST of CB322149 vs. ExPASy Swiss-Prot
Match: HSP7C_ORYLA (Heat shock cognate 71 kDa protein OS=Oryzias latipes GN=hsc70 PE=2 SV=1)

HSP 1 Score: 239.965 bits (611), Expect = 3.899e-63
Identity = 121/155 (78.06%), Postives = 130/155 (83.87%), Query Frame = 3

Query:    3 SCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFSDASVQGDMKLWPFKVIAGPADKPMIGVNYKGEEKQFAAEEISSMVLIKMREIAEAYLGATIKNAVVTVPAYFNDSQRQATKDAGVIAGLNV 467
            SCVGV+QH +VEIIANDQGNRTTPSYV FTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRF D  VQ DM  WPF VI     +P + V YKGE K F  EE+SSMVL KM+EIAEAYLG T+ NAV+TVPAYFNDSQRQATKDAG I+GLNV
Sbjct:   16 SCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFDDHVVQSDMNDWPFNVI-NDNTRPKVQVEYKGETKSFYPEEVSSMVLTKMKEIAEAYLGKTVNNAVITVPAYFNDSQRQATKDAGTISGLNV 169

[Close]

BLAST of CB322149 vs. ExPASy Swiss-Prot
Match: HSP70_SCHMA (Heat shock 70 kDa protein homolog OS=Schistosoma mansoni PE=2 SV=2)

HSP 1 Score: 239.58 bits (610), Expect = 5.092e-63
Identity = 121/155 (78.06%), Postives = 133/155 (85.81%), Query Frame = 3

Query:    3 SCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFSDASVQGDMKLWPFKVIAGPADKPMIGVNYKGEEKQFAAEEISSMVLIKMREIAEAYLGATIKNAVVTVPAYFNDSQRQATKDAGVIAGLNV 467
            SCVGV+QH +VEIIANDQGNRTTPSYV FTD+ERLIGD AKNQVAMNPTNTVFDAKRLIGRRF D SVQ DMK WPF+V      K  I V YKGE+K F+AEEISSMVL KM+E+AE+YLG T+ +AV+TVPAYFNDSQRQATKDAG IAGLNV
Sbjct:   14 SCVGVFQHGKVEIIANDQGNRTTPSYVAFTDSERLIGDGAKNQVAMNPTNTVFDAKRLIGRRFDDPSVQSDMKHWPFEV-TQVGGKLKICVEYKGEKKMFSAEEISSMVLTKMKEVAESYLGRTVSDAVITVPAYFNDSQRQATKDAGAIAGLNV 167

The following BLAST results are available for this feature:

BLAST of CB322149 vs. ExPASy Swiss-Prot

BLAST of CB322149 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
HSP72_BOVIN	7.856e-64	77.42	Heat shock-related 70 kDa protein 2 OS=Bos taurus ...	[more]
HSP70_PLAFA	7.856e-64	77.42	Heat shock 70 kDa protein OS=Plasmodium falciparum...	[more]
HSP7D_DROME	1.026e-63	78.06	Heat shock 70 kDa protein cognate 4 OS=Drosophila ...	[more]
HSP7A_DROME	1.340e-63	78.71	Heat shock 70 kDa protein cognate 1 OS=Drosophila ...	[more]
HSP70_BRUMA	1.750e-63	76.13	Heat shock 70 kDa protein OS=Brugia malayi GN=HSP7...	[more]
HSP72_MOUSE	2.286e-63	76.77	Heat shock-related 70 kDa protein 2 OS=Mus musculu...	[more]
HS71L_MACFA	2.286e-63	78.71	Heat shock 70 kDa protein 1-like OS=Macaca fascicu...	[more]
HS71A_PIG	2.286e-63	78.71	Heat shock 70 kDa protein 1A OS=Sus scrofa GN=HSPA...	[more]
HSP7C_ORYLA	3.899e-63	78.06	Heat shock cognate 71 kDa protein OS=Oryzias latip...	[more]
HSP70_SCHMA	5.092e-63	78.06	Heat shock 70 kDa protein homolog OS=Schistosoma m...	[more]

Pages

Properties


Property Name	Value
Genbank description	EST0265 Mature leaf blade cDNA subtraction library Citrus sinensis cDNA clone 24LB184 similar to HSP70 (acc# AAB88009), mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CB322149 ID=CB322149; Name=CB322149; organism=Citrus sinensis; type=EST; length=469bp
ACTCCTGCGTCGGAGTCTGGCAGCATGACAGAGTCGAGATTATCGCCAAT
GACCAAGGGAACCGGACGACGCCGTCTTATGTGGGGTTCACCGACACGGA
GCGTTTGATCGGGGACGCTGCTAAGAACCAGGTCGCCATGAACCCCACGA
ACACCGTCTTTGATGCAAAGAGGTTGATTGGTAGGAGATTCAGCGATGCA
TCAGTCCAAGGCGATATGAAGCTATGGCCATTTAAGGTGATTGCTGGACC
TGCTGACAAGCCCATGATTGGTGTTAACTACAAGGGCGAAGAGAAGCAGT
TTGCTGCTGAAGAAATCTCGTCTATGGTGCTCATCAAGATGCGTGAGATT
GCTGAGGCTTACCTTGGCGCAACTATTAAGAATGCAGTCGTTACTGTCCC
TGCTTACTTCAATGACTCACAGAGGCAGGCCACCAAGGATGCCGGTGTCA
TTGCTGGGCTTAATGTGAT