CB322149

Overview
NameCB322149
Unique NameCB322149
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length469
Libraries
Library NameType
Mature leaf blade cDNA subtraction librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CB322149 vs. ExPASy Swiss-Prot
Match: HSP73_DROME (Major heat shock 70 kDa protein Bb OS=Drosophila melanogaster GN=Hsp70Bb PE=2 SV=2)

HSP 1 Score: 237.654 bits (605), Expect = 1.935e-62
Identity = 119/155 (76.77%), Postives = 132/155 (85.16%), Query Frame = 3 
Query:    3 SCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFSDASVQGDMKLWPFKVIAGPADKPMIGVNYKGEEKQFAAEEISSMVLIKMREIAEAYLGATIKNAVVTVPAYFNDSQRQATKDAGVIAGLNV 467
            SCVGV+QH +VEIIANDQGNRTTPSYV FTD+ERLIGD AKNQVAMNP NTVFDAKRLIGR++ D  +  DMK WPFKV++    KP IGV YKGE K+FA EEISSMVL KM+E AEAYLG +I +AV+TVPAYFNDSQRQATKDAG IAGLNV
Sbjct:   13 SCVGVYQHGKVEIIANDQGNRTTPSYVAFTDSERLIGDPAKNQVAMNPRNTVFDAKRLIGRKYDDPKIAEDMKHWPFKVVS-DGGKPKIGVEYKGESKRFAPEEISSMVLTKMKETAEAYLGESITDAVITVPAYFNDSQRQATKDAGHIAGLNV 166
[Close]
BLAST of CB322149 vs. ExPASy Swiss-Prot
Match: HSP72_DROSI (Major heat shock 70 kDa protein Ba OS=Drosophila simulans GN=Hsp70Ba PE=2 SV=2)

HSP 1 Score: 237.654 bits (605), Expect = 1.935e-62
Identity = 119/155 (76.77%), Postives = 132/155 (85.16%), Query Frame = 3 
Query:    3 SCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFSDASVQGDMKLWPFKVIAGPADKPMIGVNYKGEEKQFAAEEISSMVLIKMREIAEAYLGATIKNAVVTVPAYFNDSQRQATKDAGVIAGLNV 467
            SCVGV+QH +VEIIANDQGNRTTPSYV FTD+ERLIGD AKNQVAMNP NTVFDAKRLIGR++ D  +  DMK WPFKV++    KP IGV YKGE K+FA EEISSMVL KM+E AEAYLG +I +AV+TVPAYFNDSQRQATKDAG IAGLNV
Sbjct:   13 SCVGVYQHGKVEIIANDQGNRTTPSYVAFTDSERLIGDPAKNQVAMNPRNTVFDAKRLIGRKYDDPKIAEDMKHWPFKVVS-DCGKPKIGVEYKGESKRFAPEEISSMVLAKMKETAEAYLGESITDAVITVPAYFNDSQRQATKDAGHIAGLNV 166
[Close]
BLAST of CB322149 vs. ExPASy Swiss-Prot
Match: HSP72_DROME (Major heat shock 70 kDa protein Ba OS=Drosophila melanogaster GN=Hsp70Ba PE=2 SV=2)

HSP 1 Score: 237.654 bits (605), Expect = 1.935e-62
Identity = 119/155 (76.77%), Postives = 132/155 (85.16%), Query Frame = 3 
Query:    3 SCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFSDASVQGDMKLWPFKVIAGPADKPMIGVNYKGEEKQFAAEEISSMVLIKMREIAEAYLGATIKNAVVTVPAYFNDSQRQATKDAGVIAGLNV 467
            SCVGV+QH +VEIIANDQGNRTTPSYV FTD+ERLIGD AKNQVAMNP NTVFDAKRLIGR++ D  +  DMK WPFKV++    KP IGV YKGE K+FA EEISSMVL KM+E AEAYLG +I +AV+TVPAYFNDSQRQATKDAG IAGLNV
Sbjct:   13 SCVGVYQHGKVEIIANDQGNRTTPSYVAFTDSERLIGDPAKNQVAMNPRNTVFDAKRLIGRKYDDPKIAEDMKHWPFKVVS-DGGKPKIGVEYKGESKRFAPEEISSMVLTKMKETAEAYLGESITDAVITVPAYFNDSQRQATKDAGHIAGLNV 166
[Close]
BLAST of CB322149 vs. ExPASy Swiss-Prot
Match: HSP71_DROME (Major heat shock 70 kDa protein Ab OS=Drosophila melanogaster GN=Hsp70Ab PE=1 SV=3)

HSP 1 Score: 237.654 bits (605), Expect = 1.935e-62
Identity = 119/155 (76.77%), Postives = 132/155 (85.16%), Query Frame = 3 
Query:    3 SCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFSDASVQGDMKLWPFKVIAGPADKPMIGVNYKGEEKQFAAEEISSMVLIKMREIAEAYLGATIKNAVVTVPAYFNDSQRQATKDAGVIAGLNV 467
            SCVGV+QH +VEIIANDQGNRTTPSYV FTD+ERLIGD AKNQVAMNP NTVFDAKRLIGR++ D  +  DMK WPFKV++    KP IGV YKGE K+FA EEISSMVL KM+E AEAYLG +I +AV+TVPAYFNDSQRQATKDAG IAGLNV
Sbjct:   13 SCVGVYQHGKVEIIANDQGNRTTPSYVAFTDSERLIGDPAKNQVAMNPRNTVFDAKRLIGRKYDDPKIAEDMKHWPFKVVS-DGGKPKIGVEYKGESKRFAPEEISSMVLTKMKETAEAYLGESITDAVITVPAYFNDSQRQATKDAGHIAGLNV 166
[Close]
BLAST of CB322149 vs. ExPASy Swiss-Prot
Match: HSP70_DROME (Major heat shock 70 kDa protein Aa OS=Drosophila melanogaster GN=Hsp70Aa PE=2 SV=2)

HSP 1 Score: 237.654 bits (605), Expect = 1.935e-62
Identity = 119/155 (76.77%), Postives = 132/155 (85.16%), Query Frame = 3 
Query:    3 SCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFSDASVQGDMKLWPFKVIAGPADKPMIGVNYKGEEKQFAAEEISSMVLIKMREIAEAYLGATIKNAVVTVPAYFNDSQRQATKDAGVIAGLNV 467
            SCVGV+QH +VEIIANDQGNRTTPSYV FTD+ERLIGD AKNQVAMNP NTVFDAKRLIGR++ D  +  DMK WPFKV++    KP IGV YKGE K+FA EEISSMVL KM+E AEAYLG +I +AV+TVPAYFNDSQRQATKDAG IAGLNV
Sbjct:   13 SCVGVYQHGKVEIIANDQGNRTTPSYVAFTDSERLIGDPAKNQVAMNPRNTVFDAKRLIGRKYDDPKIAEDMKHWPFKVVS-DGGKPKIGVEYKGESKRFAPEEISSMVLTKMKETAEAYLGESITDAVITVPAYFNDSQRQATKDAGHIAGLNV 166
[Close]
BLAST of CB322149 vs. ExPASy Swiss-Prot
Match: HSP71_DROSI (Major heat shock 70 kDa protein Ab OS=Drosophila simulans GN=Hsp70Ab PE=2 SV=2)

HSP 1 Score: 236.884 bits (603), Expect = 3.301e-62
Identity = 118/155 (76.13%), Postives = 132/155 (85.16%), Query Frame = 3 
Query:    3 SCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFSDASVQGDMKLWPFKVIAGPADKPMIGVNYKGEEKQFAAEEISSMVLIKMREIAEAYLGATIKNAVVTVPAYFNDSQRQATKDAGVIAGLNV 467
            SCVGV+QH  VEIIANDQGNRTTPSYV FTD+ERLIGD AKNQVAMNP NTVFDAKRLIGR++ D  +  D+K WPFKV++    KP IGV YKGE K+FA EEISSMVL+KM+E AEAYLG +I +AV+TVPAYFNDSQRQATKDAG IAGLNV
Sbjct:   13 SCVGVYQHGEVEIIANDQGNRTTPSYVAFTDSERLIGDPAKNQVAMNPRNTVFDAKRLIGRKYDDPKIAEDIKHWPFKVVS-DGGKPKIGVEYKGESKRFAPEEISSMVLVKMKETAEAYLGESITDAVITVPAYFNDSQRQATKDAGHIAGLNV 166
[Close]
BLAST of CB322149 vs. ExPASy Swiss-Prot
Match: HSP70_BRELC (Heat shock 70 kDa protein OS=Bremia lactucae GN=HSP70 PE=3 SV=1)

HSP 1 Score: 236.884 bits (603), Expect = 3.301e-62
Identity = 119/155 (76.77%), Postives = 132/155 (85.16%), Query Frame = 3 
Query:    3 SCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFSDASVQGDMKLWPFKVIAGPADKPMIGVNYKGEEKQFAAEEISSMVLIKMREIAEAYLGATIKNAVVTVPAYFNDSQRQATKDAGVIAGLNV 467
            SCVGVWQ+DRVEIIANDQGNRTTPSYV FTDTERLIGDAAKNQVAMN  NTVFDAK LIGR+FSD  VQ D+K WPFK+ +G     ++ V +KGE K F  EEISSMVLIKMRE+AEA++G  +KNAV+TVPAYFNDSQRQATKDAG IAGLNV
Sbjct:   19 SCVGVWQNDRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNAHNTVFDAKPLIGRKFSDPIVQADIKHWPFKLTSGWRQAQIV-VQFKGESKTFQPEEISSMVLIKMREVAEAFIGKEVKNAVITVPAYFNDSQRQATKDAGAIAGLNV 172
[Close]
BLAST of CB322149 vs. ExPASy Swiss-Prot
Match: HSP72_SCHPO (Probable heat shock protein ssa2 OS=Schizosaccharomyces pombe GN=ssa2 PE=1 SV=3)

HSP 1 Score: 236.498 bits (602), Expect = 4.311e-62
Identity = 120/155 (77.42%), Postives = 132/155 (85.16%), Query Frame = 3 
Query:    3 SCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFSDASVQGDMKLWPFKVIAGPADKPMIGVNYKGEEKQFAAEEISSMVLIKMREIAEAYLGATIKNAVVTVPAYFNDSQRQATKDAGVIAGLNV 467
            SCVG + ++RVEIIANDQGNRTTPSYV FTDTERLIGDAAKNQVAMNP NT+FDAKRLIGR+F D  VQ DMK WPFKVI+    KP++ V YKGE K F  EEISSMVL+KMRE AEAYLG  + +AVVTVPAYFNDSQRQATKDAG+IAGLNV
Sbjct:   14 SCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPHNTIFDAKRLIGRKFDDPEVQSDMKHWPFKVISKDG-KPVLQVEYKGETKTFTPEEISSMVLMKMRETAEAYLGGKVTDAVVTVPAYFNDSQRQATKDAGLIAGLNV 167
[Close]
BLAST of CB322149 vs. ExPASy Swiss-Prot
Match: HSP70_ECHGR (Heat shock cognate 70 kDa protein OS=Echinococcus granulosus GN=HSP70 PE=2 SV=1)

HSP 1 Score: 236.113 bits (601), Expect = 5.630e-62
Identity = 119/155 (76.77%), Postives = 132/155 (85.16%), Query Frame = 3 
Query:    3 SCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFSDASVQGDMKLWPFKVIAGPADKPMIGVNYKGEEKQFAAEEISSMVLIKMREIAEAYLGATIKNAVVTVPAYFNDSQRQATKDAGVIAGLNV 467
            SCVGV+QH +VEIIANDQGNRTTPSYV FTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRF D +VQ D+K W FKVI     KP I V Y+GE K F+AEEISSMVL+KM+E AEAYLG  + + V++VPAYFNDSQRQATKDAG I+GLNV
Sbjct:   17 SCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFDDRAVQDDIKHWAFKVI-NAGGKPKIEVEYRGETKCFSAEEISSMVLLKMKETAEAYLGKKVSDTVISVPAYFNDSQRQATKDAGTISGLNV 170
[Close]
BLAST of CB322149 vs. ExPASy Swiss-Prot
Match: HSP70_BLAEM (Heat shock 70 kDa protein OS=Blastocladiella emersonii GN=HSP70 PE=3 SV=1)

HSP 1 Score: 236.113 bits (601), Expect = 5.630e-62
Identity = 119/155 (76.77%), Postives = 128/155 (82.58%), Query Frame = 3 
Query:    3 SCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFSDASVQGDMKLWPFKVIAGPADKPMIGVNYKGEEKQFAAEEISSMVLIKMREIAEAYLGATIKNAVVTVPAYFNDSQRQATKDAGVIAGLNV 467
            SCVGVWQ+DRVEIIANDQGNRTTPSYVGFTD+ERLIGDAAKNQVAMNP NTVFDAKRLIGRRF D  VQ DMK W F V+     KP+  V +KGE K F  EE SSM+L KM+E AEAYLG  + +AVVTVPAYFNDSQRQATKDAG IAGLNV
Sbjct:   19 SCVGVWQNDRVEIIANDQGNRTTPSYVGFTDSERLIGDAAKNQVAMNPHNTVFDAKRLIGRRFDDDVVQADMKHWSFTVV-NKNSKPLFQVEFKGETKTFTPEEFSSMILTKMKETAEAYLGTKVNHAVVTVPAYFNDSQRQATKDAGAIAGLNV 172
The following BLAST results are available for this feature:
BLAST of CB322149 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
HSP73_DROME1.935e-6276.77Major heat shock 70 kDa protein Bb OS=Drosophila m... [more]
HSP72_DROSI1.935e-6276.77Major heat shock 70 kDa protein Ba OS=Drosophila s... [more]
HSP72_DROME1.935e-6276.77Major heat shock 70 kDa protein Ba OS=Drosophila m... [more]
HSP71_DROME1.935e-6276.77Major heat shock 70 kDa protein Ab OS=Drosophila m... [more]
HSP70_DROME1.935e-6276.77Major heat shock 70 kDa protein Aa OS=Drosophila m... [more]
HSP71_DROSI3.301e-6276.13Major heat shock 70 kDa protein Ab OS=Drosophila s... [more]
HSP70_BRELC3.301e-6276.77Heat shock 70 kDa protein OS=Bremia lactucae GN=HS... [more]
HSP72_SCHPO4.311e-6277.42Probable heat shock protein ssa2 OS=Schizosaccharo... [more]
HSP70_ECHGR5.630e-6276.77Heat shock cognate 70 kDa protein OS=Echinococcus ... [more]
HSP70_BLAEM5.630e-6276.77Heat shock 70 kDa protein OS=Blastocladiella emers... [more]

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Properties
Property NameValue
Genbank descriptionEST0265 Mature leaf blade cDNA subtraction library Citrus sinensis cDNA clone 24LB184 similar to HSP70 (acc# AAB88009), mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CB322149 ID=CB322149; Name=CB322149; organism=Citrus sinensis; type=EST; length=469bp
ACTCCTGCGTCGGAGTCTGGCAGCATGACAGAGTCGAGATTATCGCCAAT
GACCAAGGGAACCGGACGACGCCGTCTTATGTGGGGTTCACCGACACGGA
GCGTTTGATCGGGGACGCTGCTAAGAACCAGGTCGCCATGAACCCCACGA
ACACCGTCTTTGATGCAAAGAGGTTGATTGGTAGGAGATTCAGCGATGCA
TCAGTCCAAGGCGATATGAAGCTATGGCCATTTAAGGTGATTGCTGGACC
TGCTGACAAGCCCATGATTGGTGTTAACTACAAGGGCGAAGAGAAGCAGT
TTGCTGCTGAAGAAATCTCGTCTATGGTGCTCATCAAGATGCGTGAGATT
GCTGAGGCTTACCTTGGCGCAACTATTAAGAATGCAGTCGTTACTGTCCC
TGCTTACTTCAATGACTCACAGAGGCAGGCCACCAAGGATGCCGGTGTCA
TTGCTGGGCTTAATGTGAT
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