CB322159

Overview
NameCB322159
Unique NameCB322159
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length509
Libraries
Library NameType
Mature leaf blade cDNA subtraction librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CB322159 vs. ExPASy Swiss-Prot
Match: NEDD8_CAEEL (NEDD8 OS=Caenorhabditis elegans GN=ned-8 PE=1 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 5.060e-20
Identity = 46/76 (60.53%), Postives = 58/76 (76.32%), Query Frame = 1 
Query:  121 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 348
            M I VKTLTGK I L++E +D ++ +K K+++KEGIPP QQRLIFAGKQ+ D +T ADY +   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIELDIEPNDRVERIKEKVEEKEGIPPPQQRLIFAGKQMNDDKTAADYKVLGGSVLHLVLALRGG 76

HSP 2 Score: 71.2478 bits (173), Expect = 2.974e-12
Identity = 32/52 (61.54%), Postives = 42/52 (80.77%), Query Frame = 1 
Query:  349 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLED 504
            M I VKTLTGK I L++E +D ++ +K K+++KEGIPP QQRLIFAGKQ+ D
Sbjct:    1 MLIKVKTLTGKEIELDIEPNDRVERIKEKVEEKEGIPPPQQRLIFAGKQMND 52
[Close]
BLAST of CB322159 vs. ExPASy Swiss-Prot
Match: RUB2_ARATH (NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN=RUB2 PE=2 SV=2)

HSP 1 Score: 96.6709 bits (239), Expect = 6.609e-20
Identity = 46/75 (61.33%), Postives = 59/75 (78.67%), Query Frame = 1 
Query:  127 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 351
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DY I+  S LHLVL LRGG+
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYAIEGGSVLHLVLALRGGL 77

HSP 2 Score: 70.8626 bits (172), Expect = 3.884e-12
Identity = 32/50 (64.00%), Postives = 42/50 (84.00%), Query Frame = 1 
Query:  355 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLED 504
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLAD 52
[Close]
BLAST of CB322159 vs. ExPASy Swiss-Prot
Match: NEDD8_RAT (NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 7.307e-19
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 1 
Query:  121 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 348
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 67.3958 bits (163), Expect = 4.294e-11
Identity = 28/52 (53.85%), Postives = 42/52 (80.77%), Query Frame = 1 
Query:  349 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLED 504
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMND 52
[Close]
BLAST of CB322159 vs. ExPASy Swiss-Prot
Match: NEDD8_RABIT (NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 7.307e-19
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 1 
Query:  121 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 348
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 67.3958 bits (163), Expect = 4.294e-11
Identity = 28/52 (53.85%), Postives = 42/52 (80.77%), Query Frame = 1 
Query:  349 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLED 504
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMND 52
[Close]
BLAST of CB322159 vs. ExPASy Swiss-Prot
Match: NEDD8_MOUSE (NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2)

HSP 1 Score: 93.2041 bits (230), Expect = 7.307e-19
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 1 
Query:  121 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 348
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 67.3958 bits (163), Expect = 4.294e-11
Identity = 28/52 (53.85%), Postives = 42/52 (80.77%), Query Frame = 1 
Query:  349 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLED 504
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMND 52
[Close]
BLAST of CB322159 vs. ExPASy Swiss-Prot
Match: NEDD8_HUMAN (NEDD8 OS=Homo sapiens GN=NEDD8 PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 7.307e-19
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 1 
Query:  121 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 348
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 67.3958 bits (163), Expect = 4.294e-11
Identity = 28/52 (53.85%), Postives = 42/52 (80.77%), Query Frame = 1 
Query:  349 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLED 504
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMND 52
[Close]
BLAST of CB322159 vs. ExPASy Swiss-Prot
Match: NEDD8_BOVIN (NEDD8 OS=Bos taurus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 7.307e-19
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 1 
Query:  121 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 348
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 67.3958 bits (163), Expect = 4.294e-11
Identity = 28/52 (53.85%), Postives = 42/52 (80.77%), Query Frame = 1 
Query:  349 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLED 504
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMND 52
[Close]
BLAST of CB322159 vs. ExPASy Swiss-Prot
Match: RUB3_ORYSJ (NEDD8-like protein RUB3 OS=Oryza sativa subsp. japonica GN=RUB3 PE=3 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 9.544e-19
Identity = 41/76 (53.95%), Postives = 58/76 (76.32%), Query Frame = 1 
Query:  121 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 348
            + + V+TLTGK I +++E +DT+D +K +++++EGIPP QQRLI+ GKQL D +T  DY I+  S LHLVL LRGG
Sbjct:    1 LNVKVRTLTGKEIDIDIEMTDTVDRIKERVEEREGIPPVQQRLIYGGKQLADDKTAHDYKIEAGSVLHLVLALRGG 76

HSP 2 Score: 67.0106 bits (162), Expect = 5.608e-11
Identity = 27/52 (51.92%), Postives = 42/52 (80.77%), Query Frame = 1 
Query:  349 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLED 504
            + + V+TLTGK I +++E +DT+D +K +++++EGIPP QQRLI+ GKQL D
Sbjct:    1 LNVKVRTLTGKEIDIDIEMTDTVDRIKERVEEREGIPPVQQRLIYGGKQLAD 52
[Close]
BLAST of CB322159 vs. ExPASy Swiss-Prot
Match: RUB3_ARATH (NEDD8-like protein RUB3 OS=Arabidopsis thaliana GN=RUB3 PE=2 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 6.186e-18
Identity = 42/76 (55.26%), Postives = 57/76 (75.00%), Query Frame = 1 
Query:  121 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 348
            M+I VKTLT K I +E+E +DTI+ +K +I++KEGIPP  QR+++ GKQL D  T   YN+++ S LHLVL LRGG
Sbjct:    1 MEIKVKTLTEKQIDIEIELTDTIERIKERIEEKEGIPPVHQRIVYTGKQLADDLTAKHYNLERGSVLHLVLALRGG 76
[Close]
BLAST of CB322159 vs. ExPASy Swiss-Prot
Match: NEDD8_DICDI (NEDD8 OS=Dictyostelium discoideum GN=nedd8 PE=3 SV=2)

HSP 1 Score: 89.7373 bits (221), Expect = 8.079e-18
Identity = 41/77 (53.25%), Postives = 58/77 (75.32%), Query Frame = 1 
Query:  121 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 351
            M I VKTLTGK I ++++ +D I  +K ++++KEGIPP QQRLIF GKQ+ D +  ++Y+I+  S LHLVL LRGG+
Sbjct:    1 MLIKVKTLTGKEIEIDIDPTDKIQRIKERVEEKEGIPPSQQRLIFGGKQMGDDKPASEYSIEGGSVLHLVLALRGGL 77
The following BLAST results are available for this feature:
BLAST of CB322159 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 95
Match NameE-valueIdentityDescription
NEDD8_CAEEL5.060e-2060.53NEDD8 OS=Caenorhabditis elegans GN=ned-8 PE=1 SV=1[more]
RUB2_ARATH6.609e-2061.33NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN... [more]
NEDD8_RAT7.307e-1956.58NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1[more]
NEDD8_RABIT7.307e-1956.58NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1[more]
NEDD8_MOUSE7.307e-1956.58NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2[more]
NEDD8_HUMAN7.307e-1956.58NEDD8 OS=Homo sapiens GN=NEDD8 PE=1 SV=1[more]
NEDD8_BOVIN7.307e-1956.58NEDD8 OS=Bos taurus GN=NEDD8 PE=3 SV=1[more]
RUB3_ORYSJ9.544e-1953.95NEDD8-like protein RUB3 OS=Oryza sativa subsp. jap... [more]
RUB3_ARATH6.186e-1855.26NEDD8-like protein RUB3 OS=Arabidopsis thaliana GN... [more]
NEDD8_DICDI8.079e-1853.25NEDD8 OS=Dictyostelium discoideum GN=nedd8 PE=3 SV... [more]

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Properties
Property NameValue
Genbank descriptionEST0276 Mature leaf blade cDNA subtraction library Citrus sinensis cDNA clone 24LB196 similar to ubiquitin (acc# PS0428), mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CB322159 ID=CB322159; Name=CB322159; organism=Citrus sinensis; type=EST; length=509bp
CCACCGGACCAGCAGAGGCTGATCTTCGCAGGAAAGCAACTCGAGGACGG
CCGCACCTTGGCCGATTACAATATCCAGAAGGAATCAACTCTTCACCTCG
TGCTTCGTCTCCGTGGGGGCATGCAGATATTCGTCAAAACCCTAACGGGA
AAGACGATTACCCTCGAAGTCGAGAGCTCCGACACGATCGACAACGTCAA
GGCCAAGATCCAGGACAAGGAAGGGATTCCGCCGGACCAGCAGCGTTTGA
TCTTCGCTGGCAAGCAGCTGGAGGATGGAAGGACGCTCGCGGATTACAAC
ATTCAGAAGGAATCAACTCTTCATCTGGTTTTGCGTCTGAGGGGTGGCAT
GCAGATTTTTGTTAAGACATTGACCGGGAAGACGATTACCCTGGAGGTAG
AGAGTTCTGATACCATTGACAATGTCAAAGCCAAGATTCAGGACAAGGAA
GGGATCCCACCTGATCAGCAGAGGCTCATCTTCGCTGGGAAGCAGCTTGA
GGACGGGCG
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