CB322179

Overview
NameCB322179
Unique NameCB322179
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length425
Libraries
Library NameType
Mature leaf blade cDNA subtraction librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CB322179 vs. ExPASy Swiss-Prot
Match: DAPF_PSEAE (Diaminopimelate epimerase OS=Pseudomonas aeruginosa GN=dapF PE=3 SV=2)

HSP 1 Score: 68.5514 bits (166), Expect = 1.176e-11
Identity = 36/108 (33.33%), Postives = 51/108 (47.22%), Query Frame = -3 
Query:   79 VDKLNLAEIGPKFEHHSVFPARTNTEFVEIISPSQLKMRVWERXXXXXXXXXXXXXXXXXXXVLEGHAERRCTVDLPGGPLDIEWKEEDNHVYMTGPAEVVFYGSVLL 402
            VD   +  +GP+ E H  FP + N  F++++ P   ++RVWER                   + +G  +    +DLPGG L IEW      V MTGPA  V+ G V L
Sbjct:  169 VDSAPVHSLGPQLEVHPRFPKKANIGFLQVLDPHHARLRVWERGVGETQACGTGACAAAVAGIRQGWLQSPVQIDLPGGRLHIEWAGPGQPVMMTGPAVRVYEGQVRL 276
[Close]
BLAST of CB322179 vs. ExPASy Swiss-Prot
Match: DAPF_PSEAB (Diaminopimelate epimerase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=dapF PE=3 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 1.176e-11
Identity = 36/108 (33.33%), Postives = 51/108 (47.22%), Query Frame = -3 
Query:   79 VDKLNLAEIGPKFEHHSVFPARTNTEFVEIISPSQLKMRVWERXXXXXXXXXXXXXXXXXXXVLEGHAERRCTVDLPGGPLDIEWKEEDNHVYMTGPAEVVFYGSVLL 402
            VD   +  +GP+ E H  FP + N  F++++ P   ++RVWER                   + +G  +    +DLPGG L IEW      V MTGPA  V+ G V L
Sbjct:  169 VDSAPVHSLGPQLEVHPRFPKKANIGFLQVLDPHHARLRVWERGVGETQACGTGACAAAVAGIRQGWLQSPVQIDLPGGRLHIEWAGPGQPVMMTGPAVRVYEGQVRL 276
[Close]
BLAST of CB322179 vs. ExPASy Swiss-Prot
Match: DAPF_PSEA8 (Diaminopimelate epimerase OS=Pseudomonas aeruginosa (strain LESB58) GN=dapF PE=3 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 1.176e-11
Identity = 36/108 (33.33%), Postives = 51/108 (47.22%), Query Frame = -3 
Query:   79 VDKLNLAEIGPKFEHHSVFPARTNTEFVEIISPSQLKMRVWERXXXXXXXXXXXXXXXXXXXVLEGHAERRCTVDLPGGPLDIEWKEEDNHVYMTGPAEVVFYGSVLL 402
            VD   +  +GP+ E H  FP + N  F++++ P   ++RVWER                   + +G  +    +DLPGG L IEW      V MTGPA  V+ G V L
Sbjct:  169 VDSAPVHSLGPQLEVHPRFPKKANIGFLQVLDPHHARLRVWERGVGETQACGTGACAAAVAGIRQGWLQSPVQIDLPGGRLHIEWAGPGQPVMMTGPAVRVYEGQVRL 276
[Close]
BLAST of CB322179 vs. ExPASy Swiss-Prot
Match: DAPF_PSEA7 (Diaminopimelate epimerase OS=Pseudomonas aeruginosa (strain PA7) GN=dapF PE=3 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 1.176e-11
Identity = 36/108 (33.33%), Postives = 51/108 (47.22%), Query Frame = -3 
Query:   79 VDKLNLAEIGPKFEHHSVFPARTNTEFVEIISPSQLKMRVWERXXXXXXXXXXXXXXXXXXXVLEGHAERRCTVDLPGGPLDIEWKEEDNHVYMTGPAEVVFYGSVLL 402
            VD   +  +GP+ E H  FP + N  F++++ P   ++RVWER                   + +G  +    +DLPGG L IEW      V MTGPA  V+ G V L
Sbjct:  169 VDSAPVHSLGPQLEVHPRFPKKANIGFLQVLDPHHARLRVWERGVGETQACGTGACAAAVAGIRQGWLQSPVQIDLPGGRLHIEWAGPGQPVMMTGPAVRVYEGQVRL 276
[Close]
BLAST of CB322179 vs. ExPASy Swiss-Prot
Match: DAPF_HAES1 (Diaminopimelate epimerase OS=Haemophilus somnus (strain 129Pt) GN=dapF PE=3 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 1.176e-11
Identity = 35/108 (32.41%), Postives = 56/108 (51.85%), Query Frame = -3 
Query:   79 VDKLNLAEIGPKFEHHSVFPARTNTEFVEIISPSQLKMRVWERXXXXXXXXXXXXXXXXXXXVLEGHAERRCTVDLPGGPLDIEWKEEDNHVYMTGPAEVVFYGSVLL 402
            +   N+  +GP  E H  FP R N  F++II+ + +K+RV+ER                   +++G  +    VDLPGG L IEW+ + + +YMTG A  ++ G + L
Sbjct:  167 IQHANIEILGPLLESHERFPERVNAGFMQIINRNHIKLRVYERGAGETQACGSGACAAVAVGIMQGVLDNNVQVDLPGGRLMIEWQGKGHPLYMTGEATHIYDGVIRL 274
[Close]
BLAST of CB322179 vs. ExPASy Swiss-Prot
Match: DAPF_HAEIN (Diaminopimelate epimerase OS=Haemophilus influenzae GN=dapF PE=1 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 1.176e-11
Identity = 35/108 (32.41%), Postives = 53/108 (49.07%), Query Frame = -3 
Query:   79 VDKLNLAEIGPKFEHHSVFPARTNTEFVEIISPSQLKMRVWERXXXXXXXXXXXXXXXXXXXVLEGHAERRCTVDLPGGPLDIEWKEEDNHVYMTGPAEVVFYGSVLL 402
            +   N+ ++GP  E H  FP R N  F++II+   +K+RV+ER                   +++G       VDLPGG L IEW    + +YMTG A  ++ G + L
Sbjct:  167 IQTANVEQLGPLLESHERFPERVNAGFMQIINKEHIKLRVYERGAGETQACGSGACAAVAVGIMQGLLNNNVQVDLPGGSLMIEWNGVGHPLYMTGEATHIYDGFITL 274
[Close]
BLAST of CB322179 vs. ExPASy Swiss-Prot
Match: DAPF_HAEI8 (Diaminopimelate epimerase OS=Haemophilus influenzae (strain 86-028NP) GN=dapF PE=3 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 1.176e-11
Identity = 35/108 (32.41%), Postives = 53/108 (49.07%), Query Frame = -3 
Query:   79 VDKLNLAEIGPKFEHHSVFPARTNTEFVEIISPSQLKMRVWERXXXXXXXXXXXXXXXXXXXVLEGHAERRCTVDLPGGPLDIEWKEEDNHVYMTGPAEVVFYGSVLL 402
            +   N+ ++GP  E H  FP R N  F++II+   +K+RV+ER                   +++G       VDLPGG L IEW    + +YMTG A  ++ G + L
Sbjct:  167 IQTANVEQLGPLLESHERFPERVNAGFMQIINKEHIKLRVYERGAGETQACGSGACAAVAVGIMQGLLNNNVQVDLPGGSLMIEWNGVGHPLYMTGEATHIYDGFITL 274
[Close]
BLAST of CB322179 vs. ExPASy Swiss-Prot
Match: DAPF_TOLAT (Diaminopimelate epimerase OS=Tolumonas auensis (strain DSM 9187 / TA4) GN=dapF PE=3 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 1.535e-11
Identity = 37/100 (37.00%), Postives = 51/100 (51.00%), Query Frame = -3 
Query:   79 IGPKFEHHSVFPARTNTEFVEIISPSQLKMRVWERXXXXXXXXXXXXXXXXXXXVLEGHAERRCTVDLPGGPLDIEWKEEDNHVYMTGPAEVVFYGSVLL 378
            +GPK E    FP R N  F+E++S S++ +RV+ER                   + +   + R  V+LPGG L I W    + VYMTGPAE VF G + L
Sbjct:  177 LGPKLESFDRFPERINVGFMEVVSASEINLRVYERGAGETLACGTGACAAVVVGIQQEKLKGRVKVNLPGGSLYISWNGPGSPVYMTGPAEHVFDGQIEL 276
[Close]
BLAST of CB322179 vs. ExPASy Swiss-Prot
Match: DAPF_SHESW (Diaminopimelate epimerase OS=Shewanella sp. (strain W3-18-1) GN=dapF PE=3 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 1.535e-11
Identity = 34/103 (33.01%), Postives = 53/103 (51.46%), Query Frame = -3 
Query:   79 LAEIGPKFEHHSVFPARTNTEFVEIISPSQLKMRVWERXXXXXXXXXXXXXXXXXXXVLEGHAERRCTVDLPGGPLDIEWKEEDNHVYMTGPAEVVFYGSVLL 387
            +AEIG     H  FP   N  F+++I+   +K+RV+ER                    ++G  +++  VDLPGG L I W+ E   ++MTGPA+ V+ G + L
Sbjct:  173 VAEIGALLTKHERFPRGVNVGFMQVINSGHIKLRVYERGAAETLACGTGACAAVVVGQIQGKLDQQVRVDLPGGTLTINWEGEGKPLWMTGPAQHVYDGQIQL 275
[Close]
BLAST of CB322179 vs. ExPASy Swiss-Prot
Match: DAPF_SHEPC (Diaminopimelate epimerase OS=Shewanella putrefaciens (strain CN-32 / ATCC BAA-453) GN=dapF PE=3 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 1.535e-11
Identity = 34/103 (33.01%), Postives = 53/103 (51.46%), Query Frame = -3 
Query:   79 LAEIGPKFEHHSVFPARTNTEFVEIISPSQLKMRVWERXXXXXXXXXXXXXXXXXXXVLEGHAERRCTVDLPGGPLDIEWKEEDNHVYMTGPAEVVFYGSVLL 387
            +AEIG     H  FP   N  F+++I+   +K+RV+ER                    ++G  +++  VDLPGG L I W+ E   ++MTGPA+ V+ G + L
Sbjct:  173 VAEIGALLTKHERFPRGVNVGFMQVINSGHIKLRVYERGAAETLACGTGACAAVVVGQIQGKLDQQVRVDLPGGTLTINWEGEGKPLWMTGPAQHVYDGQIQL 275
The following BLAST results are available for this feature:
BLAST of CB322179 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 204
Match NameE-valueIdentityDescription
DAPF_PSEAE1.176e-1133.33Diaminopimelate epimerase OS=Pseudomonas aeruginos... [more]
DAPF_PSEAB1.176e-1133.33Diaminopimelate epimerase OS=Pseudomonas aeruginos... [more]
DAPF_PSEA81.176e-1133.33Diaminopimelate epimerase OS=Pseudomonas aeruginos... [more]
DAPF_PSEA71.176e-1133.33Diaminopimelate epimerase OS=Pseudomonas aeruginos... [more]
DAPF_HAES11.176e-1132.41Diaminopimelate epimerase OS=Haemophilus somnus (s... [more]
DAPF_HAEIN1.176e-1132.41Diaminopimelate epimerase OS=Haemophilus influenza... [more]
DAPF_HAEI81.176e-1132.41Diaminopimelate epimerase OS=Haemophilus influenza... [more]
DAPF_TOLAT1.535e-1137.00Diaminopimelate epimerase OS=Tolumonas auensis (st... [more]
DAPF_SHESW1.535e-1133.01Diaminopimelate epimerase OS=Shewanella sp. (strai... [more]
DAPF_SHEPC1.535e-1133.01Diaminopimelate epimerase OS=Shewanella putrefacie... [more]

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Properties
Property NameValue
Genbank descriptionEST0300 Mature leaf blade cDNA subtraction library Citrus sinensis cDNA clone 24LB226 similar to diaminopimelate epimerase (acc# NP_190926), mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CB322179 ID=CB322179; Name=CB322179; organism=Citrus sinensis; type=EST; length=425bp
ACATCTTTCTAGCTAACAATGCGCGTCTGCAAAATTCAGGTAATCTGAGA
CAAGGAAGTTCTCGACAATAAATTAATTTAGCAGGACCGATCCATAAAAC
ACTACTTCTGCCGGGCCAGTCATATATACATGGTTGTCTTCCTCCTTCCA
CTCAATATCCAATGGCCCTCCAGGTAGATCAACAGTGCATCTTCTCTCAG
CATGACCCTCAAGAACTGCTGCGACCACAACTGCACAAGCACCAGTCCCA
CATGCCAGGGTTGCTCCAGCCCCACGTTCCCAAACACGCATCTTCAGTTG
TGAAGGAGAAATAATCTCCACAAATTCTGTATTAGTTCGTGCTGGGAACA
CAGAATGATGCTCAAATTTAGGACCAATTTCAGCCAAGTTTAGTTTATCA
ACCTTCAAATTCTGCCCTTCTTTTG
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