CB322179

Overview
NameCB322179
Unique NameCB322179
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length425
Libraries
Library NameType
Mature leaf blade cDNA subtraction librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CB322179 vs. ExPASy Swiss-Prot
Match: DAPF_BACC4 (Diaminopimelate epimerase OS=Bacillus cereus (strain B4264) GN=dapF PE=3 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 3.654e-13
Identity = 43/106 (40.57%), Postives = 53/106 (50.00%), Query Frame = -3 
Query:   91 VDKLNLAEIGPKFEHHSVFPARTNTEFVEIISPSQLKMRVWERXXXXXXXXXXXXXXXXXXXVLEGHAER--RCTVDLPGGPLDIEWKEEDNHVYMTGPAEVVFYG 402
            V+K  L  +GP  E H +FP R N EF+EI++  ++  RVWER                   +L G  ER    TV L GG L I W EE N V M GPAEV+  G
Sbjct:  176 VEKAPLTTLGPVLETHEMFPERVNVEFIEILNDDEMNFRVWERGSGVTQACGTGACAAVVAAILNGKMERGKEITVHLAGGDLMIAWTEEGN-VLMKGPAEVICRG 280
[Close]
BLAST of CB322179 vs. ExPASy Swiss-Prot
Match: DAPF_BACC3 (Diaminopimelate epimerase OS=Bacillus cereus (strain 03BB102) GN=dapF PE=3 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 3.654e-13
Identity = 42/106 (39.62%), Postives = 54/106 (50.94%), Query Frame = -3 
Query:   91 VDKLNLAEIGPKFEHHSVFPARTNTEFVEIISPSQLKMRVWERXXXXXXXXXXXXXXXXXXXVLEGHAER--RCTVDLPGGPLDIEWKEEDNHVYMTGPAEVVFYG 402
            V++  L  +GP  E H +FP R N EF+EI++  ++  RVWER                   +L G  ER    TV L GG L I W EE N V M GPAEV+ +G
Sbjct:  176 VEQAPLTTLGPVLETHEMFPERVNVEFIEILNEEEMNFRVWERGSGVTQACGTGACAAVVASILNGKMERGKEITVHLAGGDLMIAWTEEGN-VLMKGPAEVICHG 280
[Close]
BLAST of CB322179 vs. ExPASy Swiss-Prot
Match: DAPF_BACC1 (Diaminopimelate epimerase OS=Bacillus cereus (strain ATCC 10987) GN=dapF PE=3 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 3.654e-13
Identity = 43/106 (40.57%), Postives = 53/106 (50.00%), Query Frame = -3 
Query:   91 VDKLNLAEIGPKFEHHSVFPARTNTEFVEIISPSQLKMRVWERXXXXXXXXXXXXXXXXXXXVLEGHAER--RCTVDLPGGPLDIEWKEEDNHVYMTGPAEVVFYG 402
            VD+  L  +GP  E H +FP R N EF+EI++  ++  RVWER                   +L G  ER    TV L GG L I W EE N V M GPAEV+  G
Sbjct:  176 VDQAPLTTLGPVLETHEMFPERVNVEFIEILNEEEMNFRVWERGSGVTQACGTGACAAVVASILNGKMERGKEITVHLAGGDLMIAWTEEGN-VLMKGPAEVICRG 280
[Close]
BLAST of CB322179 vs. ExPASy Swiss-Prot
Match: DAPF_BACAH (Diaminopimelate epimerase OS=Bacillus thuringiensis (strain Al Hakam) GN=dapF PE=3 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 3.654e-13
Identity = 42/106 (39.62%), Postives = 54/106 (50.94%), Query Frame = -3 
Query:   91 VDKLNLAEIGPKFEHHSVFPARTNTEFVEIISPSQLKMRVWERXXXXXXXXXXXXXXXXXXXVLEGHAER--RCTVDLPGGPLDIEWKEEDNHVYMTGPAEVVFYG 402
            V++  L  +GP  E H +FP R N EF+EI++  ++  RVWER                   +L G  ER    TV L GG L I W EE N V M GPAEV+ +G
Sbjct:  176 VEQAPLTTLGPVLETHEMFPERVNVEFIEILNEEEMNFRVWERGSGVTQACGTGACAAVVASILNGKMERGKEITVHLAGGDLMIAWTEEGN-VLMKGPAEVICHG 280
[Close]
BLAST of CB322179 vs. ExPASy Swiss-Prot
Match: DAPF_PHOPR (Diaminopimelate epimerase OS=Photobacterium profundum GN=dapF PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 4.773e-13
Identity = 39/111 (35.14%), Postives = 55/111 (49.55%), Query Frame = -3 
Query:   79 VDKLNLAEI---GPKFEHHSVFPARTNTEFVEIISPSQLKMRVWERXXXXXXXXXXXXXXXXXXXVLEGHAERRCTVDLPGGPLDIEWKEEDNHVYMTGPAEVVFYGSVLL 402
            VD L L ++   GP  E H  FP R N  F++++S +++K+RV+ER                    L+G  +    V LPGG L I W  E   +YMTGP   VF G ++L
Sbjct:  166 VDDLELTDVDTLGPLIESHERFPERVNAGFMQVVSRNEVKLRVYERGAGETQACGSGACAAVAVGYLQGLLDENVKVSLPGGDLHISWAGEGKSLYMTGPVAHVFDGQIVL 276
[Close]
BLAST of CB322179 vs. ExPASy Swiss-Prot
Match: DAPF_BRUSU (Diaminopimelate epimerase OS=Brucella suis GN=dapF PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 4.773e-13
Identity = 37/100 (37.00%), Postives = 50/100 (50.00%), Query Frame = -3 
Query:   88 LAEIGPKFEHHSVFPARTNTEFVEIISPSQLKMRVWERXXXXXXXXXXXXXXXXXXXVLEGHAERRCTVDLPGGPLDIEWKEEDNHVYMTGPAEVVFYGS 387
            L + GP  E+H +FP R N     + S   + +R WER                      G   R+ TV++PGGPL IEW+++D HV MTGPAE  F G+
Sbjct:  179 LDKFGPLLENHPIFPERANISIAHVTSSDTIDLRTWERGAGLTRACGSAACAAAVSAARTGRTGRKVTVNVPGGPLLIEWRDDD-HVMMTGPAEWEFSGT 277
[Close]
BLAST of CB322179 vs. ExPASy Swiss-Prot
Match: DAPF_BRUSI (Diaminopimelate epimerase OS=Brucella suis (strain ATCC 23445 / NCTC 10510) GN=dapF PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 4.773e-13
Identity = 37/100 (37.00%), Postives = 50/100 (50.00%), Query Frame = -3 
Query:   88 LAEIGPKFEHHSVFPARTNTEFVEIISPSQLKMRVWERXXXXXXXXXXXXXXXXXXXVLEGHAERRCTVDLPGGPLDIEWKEEDNHVYMTGPAEVVFYGS 387
            L + GP  E+H +FP R N     + S   + +R WER                      G   R+ TV++PGGPL IEW+++D HV MTGPAE  F G+
Sbjct:  179 LDKFGPLLENHPIFPERANISIAHVTSSDTIDLRTWERGAGLTRACGSAACAAAVSAARTGRTGRKVTVNVPGGPLLIEWRDDD-HVMMTGPAEWEFSGT 277
[Close]
BLAST of CB322179 vs. ExPASy Swiss-Prot
Match: DAPF_BRUO2 (Diaminopimelate epimerase OS=Brucella ovis (strain ATCC 25840 / 63/290 / NCTC 10512) GN=dapF PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 4.773e-13
Identity = 37/100 (37.00%), Postives = 50/100 (50.00%), Query Frame = -3 
Query:   88 LAEIGPKFEHHSVFPARTNTEFVEIISPSQLKMRVWERXXXXXXXXXXXXXXXXXXXVLEGHAERRCTVDLPGGPLDIEWKEEDNHVYMTGPAEVVFYGS 387
            L + GP  E+H +FP R N     + S   + +R WER                      G   R+ TV++PGGPL IEW+++D HV MTGPAE  F G+
Sbjct:  179 LDKFGPLLENHPIFPERANISIAHVTSSDTIDLRTWERGAGLTRACGSAACAAAVSAARTGRTGRKVTVNVPGGPLLIEWRDDD-HVMMTGPAEWEFSGT 277
[Close]
BLAST of CB322179 vs. ExPASy Swiss-Prot
Match: DAPF_BRUME (Diaminopimelate epimerase OS=Brucella melitensis GN=dapF PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 4.773e-13
Identity = 37/100 (37.00%), Postives = 50/100 (50.00%), Query Frame = -3 
Query:   88 LAEIGPKFEHHSVFPARTNTEFVEIISPSQLKMRVWERXXXXXXXXXXXXXXXXXXXVLEGHAERRCTVDLPGGPLDIEWKEEDNHVYMTGPAEVVFYGS 387
            L + GP  E+H +FP R N     + S   + +R WER                      G   R+ TV++PGGPL IEW+++D HV MTGPAE  F G+
Sbjct:  179 LDKFGPLLENHPIFPERANISIAHVTSSDTIDLRTWERGAGLTRACGSAACAAAVSAARTGRTGRKVTVNVPGGPLLIEWRDDD-HVMMTGPAEWEFSGT 277
[Close]
BLAST of CB322179 vs. ExPASy Swiss-Prot
Match: DAPF_BRUMB (Diaminopimelate epimerase OS=Brucella melitensis biotype 2 (strain ATCC 23457) GN=dapF PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 4.773e-13
Identity = 37/100 (37.00%), Postives = 50/100 (50.00%), Query Frame = -3 
Query:   88 LAEIGPKFEHHSVFPARTNTEFVEIISPSQLKMRVWERXXXXXXXXXXXXXXXXXXXVLEGHAERRCTVDLPGGPLDIEWKEEDNHVYMTGPAEVVFYGS 387
            L + GP  E+H +FP R N     + S   + +R WER                      G   R+ TV++PGGPL IEW+++D HV MTGPAE  F G+
Sbjct:  179 LDKFGPLLENHPIFPERANISIAHVTSSDTIDLRTWERGAGLTRACGSAACAAAVSAARTGRTGRKVTVNVPGGPLLIEWRDDD-HVMMTGPAEWEFSGT 277
The following BLAST results are available for this feature:
BLAST of CB322179 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 204
Match NameE-valueIdentityDescription
DAPF_BACC43.654e-1340.57Diaminopimelate epimerase OS=Bacillus cereus (stra... [more]
DAPF_BACC33.654e-1339.62Diaminopimelate epimerase OS=Bacillus cereus (stra... [more]
DAPF_BACC13.654e-1340.57Diaminopimelate epimerase OS=Bacillus cereus (stra... [more]
DAPF_BACAH3.654e-1339.62Diaminopimelate epimerase OS=Bacillus thuringiensi... [more]
DAPF_PHOPR4.773e-1335.14Diaminopimelate epimerase OS=Photobacterium profun... [more]
DAPF_BRUSU4.773e-1337.00Diaminopimelate epimerase OS=Brucella suis GN=dapF... [more]
DAPF_BRUSI4.773e-1337.00Diaminopimelate epimerase OS=Brucella suis (strain... [more]
DAPF_BRUO24.773e-1337.00Diaminopimelate epimerase OS=Brucella ovis (strain... [more]
DAPF_BRUME4.773e-1337.00Diaminopimelate epimerase OS=Brucella melitensis G... [more]
DAPF_BRUMB4.773e-1337.00Diaminopimelate epimerase OS=Brucella melitensis b... [more]

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Properties
Property NameValue
Genbank descriptionEST0300 Mature leaf blade cDNA subtraction library Citrus sinensis cDNA clone 24LB226 similar to diaminopimelate epimerase (acc# NP_190926), mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CB322179 ID=CB322179; Name=CB322179; organism=Citrus sinensis; type=EST; length=425bp
ACATCTTTCTAGCTAACAATGCGCGTCTGCAAAATTCAGGTAATCTGAGA
CAAGGAAGTTCTCGACAATAAATTAATTTAGCAGGACCGATCCATAAAAC
ACTACTTCTGCCGGGCCAGTCATATATACATGGTTGTCTTCCTCCTTCCA
CTCAATATCCAATGGCCCTCCAGGTAGATCAACAGTGCATCTTCTCTCAG
CATGACCCTCAAGAACTGCTGCGACCACAACTGCACAAGCACCAGTCCCA
CATGCCAGGGTTGCTCCAGCCCCACGTTCCCAAACACGCATCTTCAGTTG
TGAAGGAGAAATAATCTCCACAAATTCTGTATTAGTTCGTGCTGGGAACA
CAGAATGATGCTCAAATTTAGGACCAATTTCAGCCAAGTTTAGTTTATCA
ACCTTCAAATTCTGCCCTTCTTTTG
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