CB322179

Overview
NameCB322179
Unique NameCB322179
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length425
Libraries
Library NameType
Mature leaf blade cDNA subtraction librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig9292 contig Csv1_Contig9292:324..748. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CB322179 vs. ExPASy Swiss-Prot
Match: DAPF_BRUC2 (Diaminopimelate epimerase OS=Brucella canis (strain ATCC 23365 / NCTC 10854) GN=dapF PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 4.773e-13
Identity = 37/100 (37.00%), Postives = 50/100 (50.00%), Query Frame = -3 
Query:   88 LAEIGPKFEHHSVFPARTNTEFVEIISPSQLKMRVWERXXXXXXXXXXXXXXXXXXXVLEGHAERRCTVDLPGGPLDIEWKEEDNHVYMTGPAEVVFYGS 387
            L + GP  E+H +FP R N     + S   + +R WER                      G   R+ TV++PGGPL IEW+++D HV MTGPAE  F G+
Sbjct:  179 LDKFGPLLENHPIFPERANISIAHVTSSDTIDLRTWERGAGLTRACGSAACAAAVSAARTGRTGRKVTVNVPGGPLLIEWRDDD-HVMMTGPAEWEFSGT 277
[Close]
BLAST of CB322179 vs. ExPASy Swiss-Prot
Match: DAPF_BRUAB (Diaminopimelate epimerase OS=Brucella abortus GN=dapF PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 4.773e-13
Identity = 37/100 (37.00%), Postives = 50/100 (50.00%), Query Frame = -3 
Query:   88 LAEIGPKFEHHSVFPARTNTEFVEIISPSQLKMRVWERXXXXXXXXXXXXXXXXXXXVLEGHAERRCTVDLPGGPLDIEWKEEDNHVYMTGPAEVVFYGS 387
            L + GP  E+H +FP R N     + S   + +R WER                      G   R+ TV++PGGPL IEW+++D HV MTGPAE  F G+
Sbjct:  179 LDKFGPLLENHPIFPERANISIAHVTSSDTIDLRTWERGAGLTRACGSAACAAAVSAARTGRTGRKVTVNVPGGPLLIEWRDDD-HVMMTGPAEWEFSGT 277
[Close]
BLAST of CB322179 vs. ExPASy Swiss-Prot
Match: DAPF_BRUA2 (Diaminopimelate epimerase OS=Brucella abortus (strain 2308) GN=dapF PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 4.773e-13
Identity = 37/100 (37.00%), Postives = 50/100 (50.00%), Query Frame = -3 
Query:   88 LAEIGPKFEHHSVFPARTNTEFVEIISPSQLKMRVWERXXXXXXXXXXXXXXXXXXXVLEGHAERRCTVDLPGGPLDIEWKEEDNHVYMTGPAEVVFYGS 387
            L + GP  E+H +FP R N     + S   + +R WER                      G   R+ TV++PGGPL IEW+++D HV MTGPAE  F G+
Sbjct:  179 LDKFGPLLENHPIFPERANISIAHVTSSDTIDLRTWERGAGLTRACGSAACAAAVSAARTGRTGRKVTVNVPGGPLLIEWRDDD-HVMMTGPAEWEFSGT 277
[Close]
BLAST of CB322179 vs. ExPASy Swiss-Prot
Match: DAPF_BRUA1 (Diaminopimelate epimerase OS=Brucella abortus (strain S19) GN=dapF PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 4.773e-13
Identity = 37/100 (37.00%), Postives = 50/100 (50.00%), Query Frame = -3 
Query:   88 LAEIGPKFEHHSVFPARTNTEFVEIISPSQLKMRVWERXXXXXXXXXXXXXXXXXXXVLEGHAERRCTVDLPGGPLDIEWKEEDNHVYMTGPAEVVFYGS 387
            L + GP  E+H +FP R N     + S   + +R WER                      G   R+ TV++PGGPL IEW+++D HV MTGPAE  F G+
Sbjct:  179 LDKFGPLLENHPIFPERANISIAHVTSSDTIDLRTWERGAGLTRACGSAACAAAVSAARTGRTGRKVTVNVPGGPLLIEWRDDD-HVMMTGPAEWEFSGT 277
[Close]
BLAST of CB322179 vs. ExPASy Swiss-Prot
Match: DAPF_AGRRK (Diaminopimelate epimerase OS=Agrobacterium radiobacter (strain K84 / ATCC BAA-868) GN=dapF PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 4.773e-13
Identity = 38/106 (35.85%), Postives = 52/106 (49.06%), Query Frame = -3 
Query:   85 NLAEIGPKFEHHSVFPARTNTEFVEIISPSQLKMRVWERXXXXXXXXXXXXXXXXXXXVLEGHAERRCTVDLPGGP----LDIEWKEEDNHVYMTGPAEVVFYGSV 390
            +LA  GP  E+H +FP R N    +++SP+ L+ R WER                      G   R+ T+D+   P    L IEW+E D+HV MTGPAE  + G V
Sbjct:  178 DLARFGPLLENHPMFPERANITLAQVLSPTLLRTRTWERGAGLTLACGSAACSAAVSAARTGRTGRKVTIDVASAPASGQLTIEWRENDDHVVMTGPAEWEWSGVV 283
[Close]
BLAST of CB322179 vs. ExPASy Swiss-Prot
Match: DAPF_SHEFN (Diaminopimelate epimerase OS=Shewanella frigidimarina (strain NCIMB 400) GN=dapF PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 6.233e-13
Identity = 37/108 (34.26%), Postives = 55/108 (50.93%), Query Frame = -3 
Query:   79 VDKLNLAEIGPKFEHHSVFPARTNTEFVEIISPSQLKMRVWERXXXXXXXXXXXXXXXXXXXVLEGHAERRCTVDLPGGPLDIEWKEEDNHVYMTGPAEVVFYGSVLL 402
            V  +N+ EIG     H  FP   N  F+++I+P  +K+RV+ER                    L+   +++  VDLPGG L I W+ E   ++MTGPAE V+ G + L
Sbjct:  168 VQTVNVDEIGSSLTRHERFPKGVNVGFMQVINPGHIKLRVYERGAAETLACGTGACAAAAVGQLQDKLDKQVRVDLPGGSLIINWEGEGKPLWMTGPAEHVYDGQIQL 275
[Close]
BLAST of CB322179 vs. ExPASy Swiss-Prot
Match: DAPF_PROMM (Diaminopimelate epimerase OS=Prochlorococcus marinus (strain MIT 9313) GN=dapF PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 6.233e-13
Identity = 38/95 (40.00%), Postives = 49/95 (51.58%), Query Frame = -3 
Query:   91 GPKFEHHSVFPARTNTEFVEIISPSQLKMRVWERXXXXXXXXXXXXXXXXXXXVLEGHAERRCTVDLPGGPLDIEWKEEDNHVYMTGPAEVVFYG 375
            G + E H  FPA+TN  F++I SP+QL++RVWER                    L G ++    V LPGG L I W      V+MTGPAE +F G
Sbjct:  188 GQRLEKHHAFPAKTNVHFLKIHSPNQLEIRVWERGSGPTLACGTGACASLVATCLLGLSDDHAEVLLPGGVLQISWPGRRGSVFMTGPAEPIFDG 282
[Close]
BLAST of CB322179 vs. ExPASy Swiss-Prot
Match: DAPF_BACC0 (Diaminopimelate epimerase OS=Bacillus cereus (strain AH820) GN=dapF PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 6.233e-13
Identity = 42/106 (39.62%), Postives = 54/106 (50.94%), Query Frame = -3 
Query:   91 VDKLNLAEIGPKFEHHSVFPARTNTEFVEIISPSQLKMRVWERXXXXXXXXXXXXXXXXXXXVLEGHAER--RCTVDLPGGPLDIEWKEEDNHVYMTGPAEVVFYG 402
            V++  L  +GP  E H +FP R N EF+EI++  ++  RVWER                   +L G  ER    TV L GG L I W EE N V M GPAEV+ +G
Sbjct:  176 VEQAPLTTLGPVLETHEMFPERVNVEFIEILNEEEMNFRVWERGSGVTQACGTGACAAVVASILNGKMERGKEITVHLAGGDLMIAWIEEGN-VLMKGPAEVICHG 280
[Close]
BLAST of CB322179 vs. ExPASy Swiss-Prot
Match: DAPF_ACTP7 (Diaminopimelate epimerase OS=Actinobacillus pleuropneumoniae serotype 7 (strain AP76) GN=dapF PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 6.233e-13
Identity = 38/113 (33.63%), Postives = 62/113 (54.87%), Query Frame = -3 
Query:   79 LKVDKLNLA---EIGPKFEHHSVFPARTNTEFVEIISPSQLKMRVWERXXXXXXXXXXXXXXXXXXXVLEGHAERRCTVDLPGGPLDIEWKEEDNHVYMTGPAEVVFYGSVLL 408
            L+V+ +NLA   E+GP  E+H  FP R N  F+++++ + +K+RV+ER                   +++G  +    VDLPGG L IEW+   + +YMTG A  ++ G + L
Sbjct:  162 LQVEDINLAPVNELGPLLENHERFPERANIGFMQVVNRNHIKLRVFERGAGETQACGSGACGAVAVGIMQGVLDNNVQVDLPGGSLQIEWEGVGHPLYMTGDATHIYDGFIKL 274
[Close]
BLAST of CB322179 vs. ExPASy Swiss-Prot
Match: DAPF_PSEPK (Diaminopimelate epimerase OS=Pseudomonas putida (strain KT2440) GN=dapF PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 8.141e-13
Identity = 41/113 (36.28%), Postives = 56/113 (49.56%), Query Frame = -3 
Query:   79 LKVDKLNLA---EIGPKFEHHSVFPARTNTEFVEIISPSQLKMRVWERXXXXXXXXXXXXXXXXXXXVLEGHAERRCTVDLPGGPLDIEWKEEDNHVYMTGPAEVVFYGSVLL 408
            L+VD +  A   E+GPK E+H  FP R N  F+++I   +  +RVWER                   + +G  +   ++DLPGG L IEW      V MTGPA  V+ G V L
Sbjct:  164 LRVDDVQTAPVHELGPKIENHPRFPQRVNAGFIQVIDRHRANLRVWERGAGETQACGTGACAAAVAAISQGWMDSPVSLDLPGGRLHIEWAGPGKPVMMTGPAVRVYEGQVRL 276
The following BLAST results are available for this feature:
BLAST of CB322179 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 204
Match NameE-valueIdentityDescription
DAPF_BRUC24.773e-1337.00Diaminopimelate epimerase OS=Brucella canis (strai... [more]
DAPF_BRUAB4.773e-1337.00Diaminopimelate epimerase OS=Brucella abortus GN=d... [more]
DAPF_BRUA24.773e-1337.00Diaminopimelate epimerase OS=Brucella abortus (str... [more]
DAPF_BRUA14.773e-1337.00Diaminopimelate epimerase OS=Brucella abortus (str... [more]
DAPF_AGRRK4.773e-1335.85Diaminopimelate epimerase OS=Agrobacterium radioba... [more]
DAPF_SHEFN6.233e-1334.26Diaminopimelate epimerase OS=Shewanella frigidimar... [more]
DAPF_PROMM6.233e-1340.00Diaminopimelate epimerase OS=Prochlorococcus marin... [more]
DAPF_BACC06.233e-1339.62Diaminopimelate epimerase OS=Bacillus cereus (stra... [more]
DAPF_ACTP76.233e-1333.63Diaminopimelate epimerase OS=Actinobacillus pleuro... [more]
DAPF_PSEPK8.141e-1336.28Diaminopimelate epimerase OS=Pseudomonas putida (s... [more]

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Properties
Property NameValue
Genbank descriptionEST0300 Mature leaf blade cDNA subtraction library Citrus sinensis cDNA clone 24LB226 similar to diaminopimelate epimerase (acc# NP_190926), mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CB322179 ID=CB322179; Name=CB322179; organism=Citrus sinensis; type=EST; length=425bp
ACATCTTTCTAGCTAACAATGCGCGTCTGCAAAATTCAGGTAATCTGAGA
CAAGGAAGTTCTCGACAATAAATTAATTTAGCAGGACCGATCCATAAAAC
ACTACTTCTGCCGGGCCAGTCATATATACATGGTTGTCTTCCTCCTTCCA
CTCAATATCCAATGGCCCTCCAGGTAGATCAACAGTGCATCTTCTCTCAG
CATGACCCTCAAGAACTGCTGCGACCACAACTGCACAAGCACCAGTCCCA
CATGCCAGGGTTGCTCCAGCCCCACGTTCCCAAACACGCATCTTCAGTTG
TGAAGGAGAAATAATCTCCACAAATTCTGTATTAGTTCGTGCTGGGAACA
CAGAATGATGCTCAAATTTAGGACCAATTTCAGCCAAGTTTAGTTTATCA
ACCTTCAAATTCTGCCCTTCTTTTG
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