CB322194

Overview
NameCB322194
Unique NameCB322194
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length182
Libraries
Library NameType
Mature leaf blade cDNA subtraction librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig12012 contig Csv1_Contig12012:640..821. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
BLAST of CB322194 vs. ExPASy Swiss-Prot
Match: THIC_BRASO (Thiamine biosynthesis protein thiC OS=Bradyrhizobium sp. (strain ORS278) GN=thiC PE=3 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 9.617e-19
Identity = 44/58 (75.86%), Postives = 49/58 (84.48%), Query Frame = -3
Query:    7 FVRAEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASSIEEEVYKVQWA 180
            FVR E+ARGRAIIP N  H ELEPMI+GRNFL K+NANIGNSAV SS+EEEV K+ WA
Sbjct:  184 FVRDEIARGRAIIPCNINHAELEPMIIGRNFLTKINANIGNSAVTSSVEEEVDKMVWA 241          
BLAST of CB322194 vs. ExPASy Swiss-Prot
Match: THIC_ACIAD (Thiamine biosynthesis protein thiC OS=Acinetobacter sp. (strain ADP1) GN=thiC PE=3 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 9.617e-19
Identity = 44/59 (74.58%), Postives = 51/59 (86.44%), Query Frame = -3
Query:    4 FVRAEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASSIEEEVYKVQWAT 180
            FVR EVA GRAIIP+N  H E+EPMI+GRNFLVK+NANIGNSA+ SSI+EEV K+ WAT
Sbjct:  194 FVRQEVAAGRAIIPANINHPEIEPMIIGRNFLVKINANIGNSALGSSIDEEVSKMTWAT 252          
BLAST of CB322194 vs. ExPASy Swiss-Prot
Match: THIC_YERPS (Thiamine biosynthesis protein thiC OS=Yersinia pseudotuberculosis GN=thiC PE=3 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 1.256e-18
Identity = 46/59 (77.97%), Postives = 50/59 (84.75%), Query Frame = -3
Query:    4 FVRAEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASSIEEEVYKVQWAT 180
            FVR EVA GRAIIP+N  H E EPMI+GRNFLVKVNANIGNSAV SSIEEEV K+ W+T
Sbjct:  217 FVRQEVAAGRAIIPANINHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWST 275          
BLAST of CB322194 vs. ExPASy Swiss-Prot
Match: THIC_YERPE (Thiamine biosynthesis protein thiC OS=Yersinia pestis GN=thiC PE=3 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 1.256e-18
Identity = 46/59 (77.97%), Postives = 50/59 (84.75%), Query Frame = -3
Query:    4 FVRAEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASSIEEEVYKVQWAT 180
            FVR EVA GRAIIP+N  H E EPMI+GRNFLVKVNANIGNSAV SSIEEEV K+ W+T
Sbjct:  217 FVRQEVAAGRAIIPANINHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWST 275          
BLAST of CB322194 vs. ExPASy Swiss-Prot
Match: THIC_RHOPT (Thiamine biosynthesis protein thiC OS=Rhodopseudomonas palustris (strain TIE-1) GN=thiC PE=3 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 1.256e-18
Identity = 44/58 (75.86%), Postives = 50/58 (86.21%), Query Frame = -3
Query:    7 FVRAEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASSIEEEVYKVQWA 180
            FVR E+ARGRAIIP+N  H ELEPMI+GRNFL K+NANIGNSAV SS+EEEV K+ WA
Sbjct:  184 FVRDEIARGRAIIPANINHGELEPMIIGRNFLTKINANIGNSAVTSSVEEEVDKMVWA 241          
BLAST of CB322194 vs. ExPASy Swiss-Prot
Match: THIC_RHOPS (Thiamine biosynthesis protein thiC OS=Rhodopseudomonas palustris (strain BisB5) GN=thiC PE=3 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 1.256e-18
Identity = 44/58 (75.86%), Postives = 50/58 (86.21%), Query Frame = -3
Query:    7 FVRAEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASSIEEEVYKVQWA 180
            FVR E+ARGRAIIP+N  H ELEPMI+GRNFL K+NANIGNSAV SS+EEEV K+ WA
Sbjct:  184 FVRDEIARGRAIIPANINHGELEPMIIGRNFLTKINANIGNSAVTSSVEEEVDKMVWA 241          
BLAST of CB322194 vs. ExPASy Swiss-Prot
Match: THIC_RHOPA (Thiamine biosynthesis protein thiC OS=Rhodopseudomonas palustris GN=thiC PE=3 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 1.256e-18
Identity = 44/58 (75.86%), Postives = 50/58 (86.21%), Query Frame = -3
Query:    7 FVRAEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASSIEEEVYKVQWA 180
            FVR E+ARGRAIIP+N  H ELEPMI+GRNFL K+NANIGNSAV SS+EEEV K+ WA
Sbjct:  209 FVRDEIARGRAIIPANINHGELEPMIIGRNFLTKINANIGNSAVTSSVEEEVDKMVWA 266          
BLAST of CB322194 vs. ExPASy Swiss-Prot
Match: THIC_RHOP5 (Thiamine biosynthesis protein thiC OS=Rhodopseudomonas palustris (strain BisA53) GN=thiC PE=3 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 1.256e-18
Identity = 44/58 (75.86%), Postives = 50/58 (86.21%), Query Frame = -3
Query:    7 FVRAEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASSIEEEVYKVQWA 180
            FVR E+ARGRAIIP+N  H ELEPMI+GRNFL K+NANIGNSAV SS+EEEV K+ WA
Sbjct:  184 FVRDEIARGRAIIPANINHGELEPMIIGRNFLTKINANIGNSAVTSSVEEEVDKMVWA 241          
BLAST of CB322194 vs. ExPASy Swiss-Prot
Match: THIC_RHOP2 (Thiamine biosynthesis protein thiC OS=Rhodopseudomonas palustris (strain HaA2) GN=thiC PE=3 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 1.256e-18
Identity = 44/58 (75.86%), Postives = 50/58 (86.21%), Query Frame = -3
Query:    7 FVRAEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASSIEEEVYKVQWA 180
            FVR E+ARGRAIIP+N  H ELEPMI+GRNFL K+NANIGNSAV SS+EEEV K+ WA
Sbjct:  184 FVRDEIARGRAIIPANINHGELEPMIIGRNFLTKINANIGNSAVTSSVEEEVDKMVWA 241          
BLAST of CB322194 vs. ExPASy Swiss-Prot
Match: THIC_PSEPW (Thiamine biosynthesis protein thiC OS=Pseudomonas putida (strain W619) GN=thiC PE=3 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 1.256e-18
Identity = 43/57 (75.44%), Postives = 49/57 (85.96%), Query Frame = -3
Query:   10 FVRAEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASSIEEEVYKVQW 180
            FVR E+ARGRAIIP+N  H ELEPMI+GRNFLVK+N NIGNSA+ SSIEEEV K+ W
Sbjct:  195 FVREEIARGRAIIPANINHTELEPMIIGRNFLVKINGNIGNSALGSSIEEEVAKLTW 251          
The following BLAST results are available for this feature:
BLAST of CB322194 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 303
Match NameE-valueIdentityDescription
THIC_BRASO9.617e-1975.86Thiamine biosynthesis protein thiC OS=Bradyrhizobi... [more]
THIC_ACIAD9.617e-1974.58Thiamine biosynthesis protein thiC OS=Acinetobacte... [more]
THIC_YERPS1.256e-1877.97Thiamine biosynthesis protein thiC OS=Yersinia pse... [more]
THIC_YERPE1.256e-1877.97Thiamine biosynthesis protein thiC OS=Yersinia pes... [more]
THIC_RHOPT1.256e-1875.86Thiamine biosynthesis protein thiC OS=Rhodopseudom... [more]
THIC_RHOPS1.256e-1875.86Thiamine biosynthesis protein thiC OS=Rhodopseudom... [more]
THIC_RHOPA1.256e-1875.86Thiamine biosynthesis protein thiC OS=Rhodopseudom... [more]
THIC_RHOP51.256e-1875.86Thiamine biosynthesis protein thiC OS=Rhodopseudom... [more]
THIC_RHOP21.256e-1875.86Thiamine biosynthesis protein thiC OS=Rhodopseudom... [more]
THIC_PSEPW1.256e-1875.44Thiamine biosynthesis protein thiC OS=Pseudomonas ... [more]

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Properties
Property NameValue
Genbank descriptionEST0316 Mature leaf blade cDNA subtraction library Citrus sinensis cDNA clone 24LB275 similar to putative thiamine biosynthesis protein (acc# AAK26130), mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CB322194 ID=CB322194; Name=CB322194; organism=Citrus sinensis; type=EST; length=182bp
CATGGTTGCCCATTGAACCTTGTAAACTTCTTCCTCAATAGAGCTAGCTA
CAGCAGAATTACCAATATTTGCATTAACTTTGACCAAAAAATTTCTTCCG
ACTATCATTGGTTCTAGTTCCAAGTGCTTCTTGTTGGAAGGAATGATTGC
TCGGCCACGAGCAACCTCTGCCCTCACAAATT
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