CB417297

Overview
NameCB417297
Unique NameCB417297
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length590
Libraries
Library NameType
Mature peel (flavedo) cDNA subtraction librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP70_TRYCR (Heat shock 70 kDa protein OS=Trypanosoma cruzi GN=HSP70 PE=3 SV=1)

HSP 1 Score: 120.939 bits (302), Expect = 1.097e-55
Identity = 65/113 (57.52%), Postives = 74/113 (65.49%), Query Frame = 1 
Query:  250 RNARDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            R  +DAKMDK +VHDVVLVGGSTRIPKV QL+ DFF GKEL KSI PDE           IL+G  +++ +              ETAGGVMT LI RNTTIPTKK Q+FSTY
Sbjct:  322 RVLQDAKMDKRAVHDVVLVGGSTRIPKVMQLVSDFFRGKELKKSIQPDEAVAYGAAVQAFILTGGKSKQTEGLLLLDVTPLTLGIETAGGVMTSLIKRNTTIPTKKSQIFSTY 434

HSP 2 Score: 117.087 bits (292), Expect = 1.097e-55
Identity = 60/85 (70.59%), Postives = 70/85 (82.35%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNK-KDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            R+V HF  EFKRKNK KD+S N RALRRLRTACERAKRTLSS AQ TIEID+L++ +DF  TITRARFEEL  +LFR  ++PVE+
Sbjct:  238 RLVAHFTDEFKRKNKGKDLSTNLRALRRLRTACERAKRTLSSAAQATIEIDALFDNVDFQATITRARFEELCGELFRGTLQPVER 322
[Close]
BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP68_DROME (Heat shock protein 68 OS=Drosophila melanogaster GN=Hsp68 PE=1 SV=1)

HSP 1 Score: 123.635 bits (309), Expect = 1.098e-55
Identity = 59/84 (70.24%), Postives = 71/84 (84.52%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            R+VNHF +EFKRK KKD+  NPRALRRLRTA ERAKRTLSS+ + ++EID+LYEG DFY+ ++RARFEEL  DLFR  +EPVEK
Sbjct:  234 RLVNHFAEEFKRKYKKDLRSNPRALRRLRTAAERAKRTLSSSTEASLEIDALYEGHDFYSKVSRARFEELCGDLFRNTLEPVEK 317

HSP 2 Score: 114.39 bits (285), Expect = 1.098e-55
Identity = 59/110 (53.64%), Postives = 72/110 (65.45%), Query Frame = 1 
Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            +DAKMDKS +HD+VLVGGSTRIPKVQ LLQ+FF GK L  SINPDE           ILSG+ + +++D             ETAGGVMT LI RN+ IP K+ + F+TY
Sbjct:  320 KDAKMDKSQIHDIVLVGGSTRIPKVQNLLQNFFGGKTLNLSINPDEAVAYGAAIQAAILSGDKSSEIKDVLLVDVAPLSLGIETAGGVMTKLIERNSRIPCKQSKTFTTY 429
[Close]
BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP71_DROME (Major heat shock 70 kDa protein Ab OS=Drosophila melanogaster GN=Hsp70Ab PE=1 SV=3)

HSP 1 Score: 120.168 bits (300), Expect = 1.865e-55
Identity = 58/84 (69.05%), Postives = 68/84 (80.95%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            R+V H   EFKRK KKD+  NPRALRRLRTA ERAKRTLSS+ + TIEID+L+EG DFYT ++RARFEEL  DLFR  ++PVEK
Sbjct:  234 RLVTHLADEFKRKYKKDLRSNPRALRRLRTAAERAKRTLSSSTEATIEIDALFEGQDFYTKVSRARFEELCADLFRNTLQPVEK 317

HSP 2 Score: 117.087 bits (292), Expect = 1.865e-55
Identity = 62/109 (56.88%), Postives = 70/109 (64.22%), Query Frame = 1 
Query:  262 DAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            DAKMDK  +HD+VLVGGSTRIPKVQ LLQDFF+GK L  SINPDE           ILSG+ + K+QD             ETAGGVMT LI RN  IP K+ + FSTY
Sbjct:  321 DAKMDKGQIHDIVLVGGSTRIPKVQSLLQDFFHGKNLNLSINPDEAVAYGAAVQAAILSGDQSGKIQDVLLVDVAPLSLGIETAGGVMTKLIERNCRIPCKQTKTFSTY 429
[Close]
BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP70_DROME (Major heat shock 70 kDa protein Aa OS=Drosophila melanogaster GN=Hsp70Aa PE=2 SV=2)

HSP 1 Score: 120.168 bits (300), Expect = 1.865e-55
Identity = 58/84 (69.05%), Postives = 68/84 (80.95%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            R+V H   EFKRK KKD+  NPRALRRLRTA ERAKRTLSS+ + TIEID+L+EG DFYT ++RARFEEL  DLFR  ++PVEK
Sbjct:  234 RLVTHLADEFKRKYKKDLRSNPRALRRLRTAAERAKRTLSSSTEATIEIDALFEGQDFYTKVSRARFEELCADLFRNTLQPVEK 317

HSP 2 Score: 117.087 bits (292), Expect = 1.865e-55
Identity = 62/109 (56.88%), Postives = 70/109 (64.22%), Query Frame = 1 
Query:  262 DAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            DAKMDK  +HD+VLVGGSTRIPKVQ LLQDFF+GK L  SINPDE           ILSG+ + K+QD             ETAGGVMT LI RN  IP K+ + FSTY
Sbjct:  321 DAKMDKGQIHDIVLVGGSTRIPKVQSLLQDFFHGKNLNLSINPDEAVAYGAAVQAAILSGDQSGKIQDVLLVDVAPLSLGIETAGGVMTKLIERNCRIPCKQTKTFSTY 429
[Close]
BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP72_DROSI (Major heat shock 70 kDa protein Ba OS=Drosophila simulans GN=Hsp70Ba PE=2 SV=2)

HSP 1 Score: 120.939 bits (302), Expect = 3.170e-55
Identity = 58/84 (69.05%), Postives = 69/84 (82.14%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            R+V H  +EFKRK KKD+  NPRALRRLRTA ERAKRTLSS+ + TIEID+L+EG DFYT ++RARFEEL  DLFR  ++PVEK
Sbjct:  234 RLVTHLAEEFKRKYKKDLRSNPRALRRLRTAAERAKRTLSSSTEATIEIDALFEGQDFYTKVSRARFEELCADLFRNTLQPVEK 317

HSP 2 Score: 115.546 bits (288), Expect = 3.170e-55
Identity = 61/109 (55.96%), Postives = 70/109 (64.22%), Query Frame = 1 
Query:  262 DAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            DAKMDK  +HD+VLVGGSTRIPKVQ LLQ+FF+GK L  SINPDE           ILSG+ + K+QD             ETAGGVMT LI RN  IP K+ + FSTY
Sbjct:  321 DAKMDKGQIHDIVLVGGSTRIPKVQSLLQEFFHGKNLNLSINPDEAVAYGAAVQAAILSGDQSGKIQDVLLVDVAPLSLGIETAGGVMTKLIERNCRIPCKQTKTFSTY 429
[Close]
BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP75_DROME (Major heat shock 70 kDa protein Bc OS=Drosophila melanogaster GN=Hsp70Bc PE=2 SV=2)

HSP 1 Score: 120.939 bits (302), Expect = 3.170e-55
Identity = 58/84 (69.05%), Postives = 69/84 (82.14%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            R+V H  +EFKRK KKD+  NPRALRRLRTA ERAKRTLSS+ + TIEID+L+EG DFYT ++RARFEEL  DLFR  ++PVEK
Sbjct:  234 RLVTHLAEEFKRKYKKDLRSNPRALRRLRTAAERAKRTLSSSTEATIEIDALFEGQDFYTKVSRARFEELCADLFRNTLQPVEK 317

HSP 2 Score: 115.546 bits (288), Expect = 3.170e-55
Identity = 61/109 (55.96%), Postives = 70/109 (64.22%), Query Frame = 1 
Query:  262 DAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            DAKMDK  +HD+VLVGGSTRIPKVQ LLQ+FF+GK L  SINPDE           ILSG+ + K+QD             ETAGGVMT LI RN  IP K+ + FSTY
Sbjct:  321 DAKMDKGQIHDIVLVGGSTRIPKVQSLLQEFFHGKNLNLSINPDEAVAYGAAVQAAILSGDQSGKIQDVLLVDVAPLSLGIETAGGVMTKLIERNCRIPCKQTKTFSTY 429
[Close]
BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP74_DROME (Major heat shock 70 kDa protein Bbb OS=Drosophila melanogaster GN=Hsp70Bbb PE=2 SV=2)

HSP 1 Score: 120.939 bits (302), Expect = 3.170e-55
Identity = 58/84 (69.05%), Postives = 69/84 (82.14%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            R+V H  +EFKRK KKD+  NPRALRRLRTA ERAKRTLSS+ + TIEID+L+EG DFYT ++RARFEEL  DLFR  ++PVEK
Sbjct:  234 RLVTHLAEEFKRKYKKDLRSNPRALRRLRTAAERAKRTLSSSTEATIEIDALFEGQDFYTKVSRARFEELCADLFRNTLQPVEK 317

HSP 2 Score: 115.546 bits (288), Expect = 3.170e-55
Identity = 61/109 (55.96%), Postives = 70/109 (64.22%), Query Frame = 1 
Query:  262 DAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            DAKMDK  +HD+VLVGGSTRIPKVQ LLQ+FF+GK L  SINPDE           ILSG+ + K+QD             ETAGGVMT LI RN  IP K+ + FSTY
Sbjct:  321 DAKMDKGQIHDIVLVGGSTRIPKVQSLLQEFFHGKNLNLSINPDEAVAYGAAVQAAILSGDQSGKIQDVLLVDVAPLSLGIETAGGVMTKLIERNCRIPCKQTKTFSTY 429
[Close]
BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP73_DROME (Major heat shock 70 kDa protein Bb OS=Drosophila melanogaster GN=Hsp70Bb PE=2 SV=2)

HSP 1 Score: 120.939 bits (302), Expect = 3.170e-55
Identity = 58/84 (69.05%), Postives = 69/84 (82.14%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            R+V H  +EFKRK KKD+  NPRALRRLRTA ERAKRTLSS+ + TIEID+L+EG DFYT ++RARFEEL  DLFR  ++PVEK
Sbjct:  234 RLVTHLAEEFKRKYKKDLRSNPRALRRLRTAAERAKRTLSSSTEATIEIDALFEGQDFYTKVSRARFEELCADLFRNTLQPVEK 317

HSP 2 Score: 115.546 bits (288), Expect = 3.170e-55
Identity = 61/109 (55.96%), Postives = 70/109 (64.22%), Query Frame = 1 
Query:  262 DAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            DAKMDK  +HD+VLVGGSTRIPKVQ LLQ+FF+GK L  SINPDE           ILSG+ + K+QD             ETAGGVMT LI RN  IP K+ + FSTY
Sbjct:  321 DAKMDKGQIHDIVLVGGSTRIPKVQSLLQEFFHGKNLNLSINPDEAVAYGAAVQAAILSGDQSGKIQDVLLVDVAPLSLGIETAGGVMTKLIERNCRIPCKQTKTFSTY 429
[Close]
BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP72_DROME (Major heat shock 70 kDa protein Ba OS=Drosophila melanogaster GN=Hsp70Ba PE=2 SV=2)

HSP 1 Score: 120.939 bits (302), Expect = 3.170e-55
Identity = 58/84 (69.05%), Postives = 69/84 (82.14%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            R+V H  +EFKRK KKD+  NPRALRRLRTA ERAKRTLSS+ + TIEID+L+EG DFYT ++RARFEEL  DLFR  ++PVEK
Sbjct:  234 RLVTHLAEEFKRKYKKDLRSNPRALRRLRTAAERAKRTLSSSTEATIEIDALFEGQDFYTKVSRARFEELCADLFRNTLQPVEK 317

HSP 2 Score: 115.546 bits (288), Expect = 3.170e-55
Identity = 61/109 (55.96%), Postives = 70/109 (64.22%), Query Frame = 1 
Query:  262 DAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            DAKMDK  +HD+VLVGGSTRIPKVQ LLQ+FF+GK L  SINPDE           ILSG+ + K+QD             ETAGGVMT LI RN  IP K+ + FSTY
Sbjct:  321 DAKMDKGQIHDIVLVGGSTRIPKVQSLLQEFFHGKNLNLSINPDEAVAYGAAVQAAILSGDQSGKIQDVLLVDVAPLSLGIETAGGVMTKLIERNCRIPCKQTKTFSTY 429
[Close]
BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP71_DROSI (Major heat shock 70 kDa protein Ab OS=Drosophila simulans GN=Hsp70Ab PE=2 SV=2)

HSP 1 Score: 120.939 bits (302), Expect = 4.133e-55
Identity = 58/84 (69.05%), Postives = 69/84 (82.14%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            R+V H  +EFKRK KKD+  NPRALRRLRTA ERAKRTLSS+ + TIEID+L+EG DFYT ++RARFEEL  DLFR  ++PVEK
Sbjct:  234 RLVTHLAEEFKRKYKKDLRSNPRALRRLRTAAERAKRTLSSSTEATIEIDALFEGQDFYTKVSRARFEELCADLFRNTLQPVEK 317

HSP 2 Score: 115.161 bits (287), Expect = 4.133e-55
Identity = 61/109 (55.96%), Postives = 69/109 (63.30%), Query Frame = 1 
Query:  262 DAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            DAKMDK  +HD+VLVGGSTRIPKVQ LLQ+FF+GK L  SINPDE           ILSG+   K+QD             ETAGGVMT LI RN  IP K+ + FSTY
Sbjct:  321 DAKMDKGQIHDIVLVGGSTRIPKVQSLLQEFFHGKNLNLSINPDEAVAYGAAVQAAILSGDQTGKIQDVLLVDVAPLSLGIETAGGVMTKLIERNCRIPCKQTKTFSTY 429
The following BLAST results are available for this feature:
BLAST of CB417297 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
HSP70_TRYCR1.097e-5557.52Heat shock 70 kDa protein OS=Trypanosoma cruzi GN=... [more]
HSP68_DROME1.098e-5570.24Heat shock protein 68 OS=Drosophila melanogaster G... [more]
HSP71_DROME1.865e-5569.05Major heat shock 70 kDa protein Ab OS=Drosophila m... [more]
HSP70_DROME1.865e-5569.05Major heat shock 70 kDa protein Aa OS=Drosophila m... [more]
HSP72_DROSI3.170e-5569.05Major heat shock 70 kDa protein Ba OS=Drosophila s... [more]
HSP75_DROME3.170e-5569.05Major heat shock 70 kDa protein Bc OS=Drosophila m... [more]
HSP74_DROME3.170e-5569.05Major heat shock 70 kDa protein Bbb OS=Drosophila ... [more]
HSP73_DROME3.170e-5569.05Major heat shock 70 kDa protein Bb OS=Drosophila m... [more]
HSP72_DROME3.170e-5569.05Major heat shock 70 kDa protein Ba OS=Drosophila m... [more]
HSP71_DROSI4.133e-5569.05Major heat shock 70 kDa protein Ab OS=Drosophila s... [more]

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Properties
Property NameValue
Genbank descriptionEST0370 Mature peel (flavedo) cDNA subtraction library Citrus sinensis cDNA clone 6P015 similar to 70 kDa heat shock protein (acc# AAL24367), mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CB417297 ID=CB417297; Name=CB417297; organism=Citrus sinensis; type=EST; length=590bp
ACAGAATGGTGAACCACTTTGTTCAAGAGTTCAAGAGGAAAAACAAGAAG
GATATTAGTGGAAACCCCAGAGCGTTGAGGAGGTTGAGAACTGCCTGTGA
AAGGGCTAAGAGGACCCTGTCATCAACCGCACAGACTACAATTGAGATTG
ATTCTTTATATGAGGGTATTGATTTCTACACAACCATTACAAGGGCTAGA
TTCGAAGAGTTGAACATGGATTTGTTCAGGAAGTGTATGGAGCCAGTGGA
GAAATGCGAGAGATGCTAAGATGGACAAGAGCAGTGTCCATGATGTGGTT
CTTGTTGGTGGGTCTACTAGGATCCCAAAAGTCCAGCAACTGTTGCAGGA
CTTCTTCAATGGAAAAGAGCTATGCAAGAGCATCAACCCTGATGAAGCGG
TTGCCTACGGTGCTGCTGTGCAGGCTGCAATCTTGAGTGGTGAGGGTAAT
GAGAAGGTTCAGGACCTCTTATTGCTTGACGTCACCCCTCTATCGCTGGG
TTTGGAGACTGCTGGTGGAGTCATGACTGTGTTGATTCCAAGGAACACCA
CTATTCCAACCAAGAAGGAGCAGGTGTTCTCCACATACTC
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