CB417297 | Citrus Genome Database

Overview


Name	CB417297
Unique Name	CB417297
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	590

Libraries


Library Name	Type
Mature peel (flavedo) cDNA subtraction library	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig13846	contig	Csv1_Contig13846:800..1391.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP70_LEIDO (Heat shock 70 kDa protein OS=Leishmania donovani GN=HSP70 PE=3 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 7.022e-55
Identity = 67/113 (59.29%), Postives = 75/113 (66.37%), Query Frame = 1

Query:  250 RNARDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            R  +DAKMDK SVHDVVLVGGSTRIPKVQ L+ DFF GKEL KSINPDE           IL+G  +++ +              ETAGGVMT LI RNTTIPTKK Q+FSTY
Sbjct:  321 RVLQDAKMDKRSVHDVVLVGGSTRIPKVQSLVSDFFGGKELNKSINPDEAVAYGAAVQAFILTGGKSKQTEGLLLLDVTPLTLGIETAGGVMTALIKRNTTIPTKKSQIFSTY 433

HSP 2 Score: 110.153 bits (274), Expect = 7.022e-55
Identity = 56/85 (65.88%), Postives = 68/85 (80.00%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNK-KDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            R+V  F +EFKRKNK K+++ + RALR LRTACERAKRTLSS  Q TIEID+L+E +DF  TITRARFEEL  DLFR  ++PVE+
Sbjct:  237 RLVTFFTEEFKRKNKGKNLASSHRALRGLRTACERAKRTLSSATQATIEIDALFENVDFQATITRARFEELCGDLFRSTIQPVER 321

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP70_LEIAM (Heat shock 70 kDa protein OS=Leishmania amazonensis GN=HSP70 PE=2 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 2.028e-54
Identity = 67/113 (59.29%), Postives = 75/113 (66.37%), Query Frame = 1

Query:  250 RNARDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            R  +DAKMDK SVHDVVLVGGSTRIPKVQ L+ DFF GKEL KSINPDE           IL+G  +++ +              ETAGGVMT LI RNTTIPTKK Q+FSTY
Sbjct:  322 RVLQDAKMDKRSVHDVVLVGGSTRIPKVQSLVSDFFGGKELNKSINPDEAVAYGAAVQAFILTGGKSKQTEGLLLLDVTPLTLGIETAGGVMTALIKRNTTIPTKKSQIFSTY 434

HSP 2 Score: 108.612 bits (270), Expect = 2.028e-54
Identity = 54/85 (63.53%), Postives = 68/85 (80.00%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNK-KDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            R+V  F +EFKRKNK K+++ + R+LRRLRTACERAKRTLSS  Q TIEID+L++ +DF  TI RARFEEL  DLFR  ++PVE+
Sbjct:  238 RLVTFFTEEFKRKNKGKNLASSHRSLRRLRTACERAKRTLSSATQATIEIDALFDNVDFQATINRARFEELCGDLFRSTIQPVER 322

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP70_CERCA (Heat shock protein 70 OS=Ceratitis capitata PE=2 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 2.875e-53
Identity = 59/84 (70.24%), Postives = 71/84 (84.52%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            R+V+H  +EFKRK KKD+  NPRALRRLRTA ERAKRTLSS+ + TIEID+L+EGID YT ++RARFEEL  DLFR+ +EPVEK
Sbjct:  233 RLVSHLAEEFKRKYKKDLRSNPRALRRLRTAAERAKRTLSSSTEATIEIDALFEGIDLYTKVSRARFEELCADLFRQTLEPVEK 316

HSP 2 Score: 106.686 bits (265), Expect = 2.875e-53
Identity = 58/109 (53.21%), Postives = 67/109 (61.47%), Query Frame = 1

Query:  262 DAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            DAKMDK+ +H  VLVGGSTRIPKVQ+LLQ FF GK L  SINPDE           ILSG+ + ++QD             ETAGGVM  +I RN  IP K+ Q FSTY
Sbjct:  320 DAKMDKNQIHVYVLVGGSTRIPKVQRLLQSFFCGKSLNLSINPDEAVAYGAAVQAAILSGDKSTEIQDVLLVDVAPLSLGIETAGGVMAKIIERNCRIPCKQTQTFSTY 428

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP70_THEPA (Heat shock 70 kDa protein OS=Theileria parva GN=TP02_0148 PE=2 SV=2)

HSP 1 Score: 122.865 bits (307), Expect = 1.410e-52
Identity = 61/105 (58.10%), Postives = 74/105 (70.48%), Query Frame = 1

Query:  271 MDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DK S+H+VVLVGGSTRIPK+Q L+++FFNGKE C+SINPDE           ILSG  +EK+Q+             ETAGGVMTVLI RNTTIPTKK Q+F+T
Sbjct:  327 LDKRSIHEVVLVGGSTRIPKIQTLIKNFFNGKEPCRSINPDEAVAYGAAVQAAILSGNQSEKIQELLLLDVAPLSLGLETAGGVMTVLIKRNTTIPTKKNQIFTT 431

HSP 2 Score: 104.76 bits (260), Expect = 1.410e-52
Identity = 55/84 (65.48%), Postives = 65/84 (77.38%), Query Frame = 3

Query:    6 MVNHFVQEFKRKNK-KDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            +V H V++F R N  K+IS N RALRRLRT CERAKR LSS+ Q TIE+DSLYEGID+ TTI+RARFEEL  + FR  + PVEK
Sbjct:  237 LVEHCVRDFMRLNNGKNISSNKRALRRLRTHCERAKRVLSSSTQATIELDSLYEGIDYNTTISRARFEELCNEKFRSTLVPVEK 320

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP70_THEAN (Heat shock 70 kDa protein OS=Theileria annulata GN=TA11610 PE=3 SV=2)

HSP 1 Score: 122.865 bits (307), Expect = 1.410e-52
Identity = 61/105 (58.10%), Postives = 74/105 (70.48%), Query Frame = 1

Query:  271 MDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DK S+H+VVLVGGSTRIPK+Q L+++FFNGKE C+SINPDE           ILSG  +EK+Q+             ETAGGVMTVLI RNTTIPTKK Q+F+T
Sbjct:  327 LDKRSIHEVVLVGGSTRIPKIQTLIKNFFNGKEPCRSINPDEAVAYGAAVQAAILSGNQSEKIQELLLLDVAPLSLGLETAGGVMTVLIKRNTTIPTKKNQIFTT 431

HSP 2 Score: 104.76 bits (260), Expect = 1.410e-52
Identity = 55/84 (65.48%), Postives = 65/84 (77.38%), Query Frame = 3

Query:    6 MVNHFVQEFKRKNK-KDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            +V H V++F R N  K+IS N RALRRLRT CERAKR LSS+ Q TIE+DSLYEGID+ TTI+RARFEEL  + FR  + PVEK
Sbjct:  237 LVEHCVRDFMRLNNGKNISSNKRALRRLRTHCERAKRVLSSSTQATIELDSLYEGIDYNTTISRARFEELCNEKFRSTLVPVEK 320

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP70_MIMIV (Heat shock 70 kDa protein homolog OS=Acanthamoeba polyphaga mimivirus GN=HSP70 PE=3 SV=1)

HSP 1 Score: 115.161 bits (287), Expect = 1.839e-52
Identity = 57/109 (52.29%), Postives = 76/109 (69.72%), Query Frame = 1

Query:  262 DAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            D+K+DKS VH++VLVGGS+RIPKV+QLL +FFNGK+L +++NPDE           IL G+ +EK+Q+             ETAGG+MT +I RNTTIP KK +VF+TY
Sbjct:  323 DSKLDKSQVHEIVLVGGSSRIPKVRQLLSNFFNGKKLNETVNPDEAVAYGAAIQAAILVGQTDEKLQNIVLVDVTPLSLGLETAGGIMTNIIDRNTTIPCKKSRVFTTY 431

HSP 2 Score: 112.079 bits (279), Expect = 1.839e-52
Identity = 55/84 (65.48%), Postives = 69/84 (82.14%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            R+V   V++FKRK K D++ + +ALRRLRTACERAKR LSS+ QTTIE+DSL+EGID+  T+TRA+FEEL  DLFR  +EPVEK
Sbjct:  236 RLVTWCVEDFKRKYKTDLNQSAKALRRLRTACERAKRALSSSTQTTIEVDSLFEGIDYNVTLTRAKFEELCSDLFRAGLEPVEK 319

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP7X_PIG (Heat shock 70 kDa protein (Fragment) OS=Sus scrofa PE=2 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 7.555e-51
Identity = 63/107 (58.88%), Postives = 73/107 (68.22%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVF 579
            RDAK+DK+ +HD+VLVGGSTRIPK+Q+LLQDFFNG+EL KSINPDE           +L G+  EKVQD             ETAGGVMT LI RN TIPTK+ Q F
Sbjct:   62 RDAKLDKAQIHDIVLVGGSTRIPKIQKLLQDFFNGRELNKSINPDEAVAYGAAVQAAVLMGDKCEKVQDLLLLDVAPLSLGLETAGGVMTTLIQRNATIPTKQTQNF 168

HSP 2 Score: 98.9821 bits (245), Expect = 7.555e-51
Identity = 48/62 (77.42%), Postives = 54/62 (87.10%), Query Frame = 3

Query:   78 RRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCER 263
            RRLRTACERAKRTLSS+ Q T+EIDSL+EG+DFYT+ITRARFEEL  DLFR  +EPVEK  R
Sbjct:    1 RRLRTACERAKRTLSSSTQATLEIDSLFEGVDFYTSITRARFEELCSDLFRSTLEPVEKALR 62

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: BIP_SPIOL (Luminal-binding protein OS=Spinacia oleracea GN=HSC70 PE=2 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 6.261e-50
Identity = 60/109 (55.05%), Postives = 73/109 (66.97%), Query Frame = 1

Query:  262 DAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            DA ++K+ + ++VLVGGSTRIPKVQQLL++FFNGKE  K +NPDE           ILSGEG E+ ++             ET GGVMT LIPRNT IPTKK QVF+TY
Sbjct:  355 DAGLEKNQIDEIVLVGGSTRIPKVQQLLKEFFNGKEPSKGVNPDEAVAFGAAVQGSILSGEGGEETKEILLLDVAPLTLGIETVGGVMTKLIPRNTVIPTKKSQVFTTY 463

HSP 2 Score: 98.9821 bits (245), Expect = 6.261e-50
Identity = 48/84 (57.14%), Postives = 64/84 (76.19%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            R++ +F++  K+K+ KDIS + RAL +LR  CERAKR LSS  Q  +EI+SL++G+DF   +TRARFEELN DLFRK M PV+K
Sbjct:  268 RLMEYFIKLIKKKHTKDISKDNRALGKLRRECERAKRALSSQHQVRVEIESLFDGVDFSEPLTRARFEELNNDLFRKTMGPVKK 351

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: BIP1_ARATH (Luminal-binding protein 1 OS=Arabidopsis thaliana GN=At5g28540 PE=1 SV=1)

HSP 1 Score: 119.013 bits (297), Expect = 1.807e-49
Identity = 61/109 (55.96%), Postives = 71/109 (65.14%), Query Frame = 1

Query:  262 DAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            DA + KS + ++VLVGGSTRIPKVQQLL+DFF GKE  K +NPDE           ILSGEG ++ +D             ET GGVMT LIPRNT IPTKK QVF+TY
Sbjct:  354 DAGLQKSQIDEIVLVGGSTRIPKVQQLLKDFFEGKEPNKGVNPDEAVAYGAAVQGGILSGEGGDETKDILLLDVAPLTLGIETVGGVMTKLIPRNTVIPTKKSQVFTTY 462

HSP 2 Score: 98.2117 bits (243), Expect = 1.807e-49
Identity = 47/84 (55.95%), Postives = 65/84 (77.38%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            R++ +F++  K+K++KDIS + +AL +LR  CERAKR LSS  Q  +EI+SL++G+DF   +TRARFEELN DLFRK M PV+K
Sbjct:  267 RVMEYFIKLIKKKHQKDISKDNKALGKLRRECERAKRALSSQHQVRVEIESLFDGVDFSEPLTRARFEELNNDLFRKTMGPVKK 350

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: BIP3_MAIZE (Luminal-binding protein 3 OS=Zea mays GN=BIPE3 PE=2 SV=1)

HSP 1 Score: 124.405 bits (311), Expect = 4.002e-49
Identity = 62/109 (56.88%), Postives = 74/109 (67.89%), Query Frame = 1

Query:  262 DAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            DA ++KS +H++VLVGGSTRIPKVQQLL+D+FNGKE  K +NPDE           ILSGEG ++ +D             ET GGVMT LIPRNT IPTKK QVF+TY
Sbjct:  352 DAGLEKSQIHEIVLVGGSTRIPKVQQLLKDYFNGKEPNKGVNPDEAVAFGAAVQGSILSGEGGDETKDILLLDVAPLTLGIETVGGVMTKLIPRNTVIPTKKSQVFTTY 460

HSP 2 Score: 91.6633 bits (226), Expect = 4.002e-49
Identity = 46/84 (54.76%), Postives = 61/84 (72.62%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            R++ +F++  K+K  KDIS + RAL +LR   ERAKR LS+  Q  +EI+SL++G DF   +TRARFEELN DLFRK M PV+K
Sbjct:  265 RIMEYFIKLIKKKYSKDISKDNRALGKLRREAERAKRALSNQHQVRVEIESLFDGTDFSEPLTRARFEELNNDLFRKTMGPVKK 348

The following BLAST results are available for this feature:

BLAST of CB417297 vs. ExPASy Swiss-Prot

BLAST of CB417297 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
HSP70_LEIDO	7.022e-55	59.29	Heat shock 70 kDa protein OS=Leishmania donovani G...	[more]
HSP70_LEIAM	2.028e-54	59.29	Heat shock 70 kDa protein OS=Leishmania amazonensi...	[more]
HSP70_CERCA	2.875e-53	70.24	Heat shock protein 70 OS=Ceratitis capitata PE=2 S...	[more]
HSP70_THEPA	1.410e-52	58.10	Heat shock 70 kDa protein OS=Theileria parva GN=TP...	[more]
HSP70_THEAN	1.410e-52	58.10	Heat shock 70 kDa protein OS=Theileria annulata GN...	[more]
HSP70_MIMIV	1.839e-52	52.29	Heat shock 70 kDa protein homolog OS=Acanthamoeba ...	[more]
HSP7X_PIG	7.555e-51	58.88	Heat shock 70 kDa protein (Fragment) OS=Sus scrofa...	[more]
BIP_SPIOL	6.261e-50	55.05	Luminal-binding protein OS=Spinacia oleracea GN=HS...	[more]
BIP1_ARATH	1.807e-49	55.96	Luminal-binding protein 1 OS=Arabidopsis thaliana ...	[more]
BIP3_MAIZE	4.002e-49	56.88	Luminal-binding protein 3 OS=Zea mays GN=BIPE3 PE=...	[more]

Pages

Properties


Property Name	Value
Genbank description	EST0370 Mature peel (flavedo) cDNA subtraction library Citrus sinensis cDNA clone 6P015 similar to 70 kDa heat shock protein (acc# AAL24367), mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CB417297 ID=CB417297; Name=CB417297; organism=Citrus sinensis; type=EST; length=590bp
ACAGAATGGTGAACCACTTTGTTCAAGAGTTCAAGAGGAAAAACAAGAAG
GATATTAGTGGAAACCCCAGAGCGTTGAGGAGGTTGAGAACTGCCTGTGA
AAGGGCTAAGAGGACCCTGTCATCAACCGCACAGACTACAATTGAGATTG
ATTCTTTATATGAGGGTATTGATTTCTACACAACCATTACAAGGGCTAGA
TTCGAAGAGTTGAACATGGATTTGTTCAGGAAGTGTATGGAGCCAGTGGA
GAAATGCGAGAGATGCTAAGATGGACAAGAGCAGTGTCCATGATGTGGTT
CTTGTTGGTGGGTCTACTAGGATCCCAAAAGTCCAGCAACTGTTGCAGGA
CTTCTTCAATGGAAAAGAGCTATGCAAGAGCATCAACCCTGATGAAGCGG
TTGCCTACGGTGCTGCTGTGCAGGCTGCAATCTTGAGTGGTGAGGGTAAT
GAGAAGGTTCAGGACCTCTTATTGCTTGACGTCACCCCTCTATCGCTGGG
TTTGGAGACTGCTGGTGGAGTCATGACTGTGTTGATTCCAAGGAACACCA
CTATTCCAACCAAGAAGGAGCAGGTGTTCTCCACATACTC