CB417297 | Citrus Genome Database

Overview


Name	CB417297
Unique Name	CB417297
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	590

Libraries


Library Name	Type
Mature peel (flavedo) cDNA subtraction library	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig13846	contig	Csv1_Contig13846:800..1391.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP7C_DANRE (Heat shock cognate 71 kDa protein OS=Danio rerio GN=hspa8 PE=2 SV=1)

HSP 1 Score: 140.584 bits (353), Expect = 4.292e-67
Identity = 69/87 (79.31%), Postives = 77/87 (88.51%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCER 263
            RMVNHF+ EFKRK+KKDIS N RA+RRLRTACERAKRTLSS+ Q +IEIDSLYEGIDFYT+ITRARFEELN DLFR  ++PVEK  R
Sbjct:  236 RMVNHFITEFKRKHKKDISDNKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALR 322

HSP 2 Score: 135.576 bits (340), Expect = 4.292e-67
Identity = 69/110 (62.73%), Postives = 79/110 (71.82%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            RDAKMDK+ +HD+VLVGGSTRIPK+Q+LLQD+FNGKEL KSINPDE           ILSG+ +E VQD             ETAGGVMTVLI RNTTIPTK+ Q F+TY
Sbjct:  322 RDAKMDKAQIHDIVLVGGSTRIPKIQKLLQDYFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTY 431

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP72_SCHPO (Probable heat shock protein ssa2 OS=Schizosaccharomyces pombe GN=ssa2 PE=1 SV=3)

HSP 1 Score: 145.206 bits (365), Expect = 9.517e-67
Identity = 71/87 (81.61%), Postives = 80/87 (91.95%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCER 263
            R+VNHF+QEFKRKNKKDI+GN RA+RRLRTACERAKRTLSS+AQ +IEIDSL+EGIDFYT+ITRARFEEL  DLFRK MEPVE+  R
Sbjct:  234 RLVNHFIQEFKRKNKKDITGNARAVRRLRTACERAKRTLSSSAQASIEIDSLFEGIDFYTSITRARFEELCADLFRKTMEPVERVLR 320

HSP 2 Score: 129.798 bits (325), Expect = 9.517e-67
Identity = 65/113 (57.52%), Postives = 80/113 (70.80%), Query Frame = 1

Query:  250 RNARDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            R  RD+K+DK+SV+++VLVGGSTRIP+VQ+L+ DFFNGKE CKSINPDE           +L+G+ +EK QD             ETAGGVMT LI RNTTIPTKK ++FSTY
Sbjct:  317 RVLRDSKVDKASVNEIVLVGGSTRIPRVQKLVSDFFNGKEPCKSINPDEAVAYGAAVQAAVLTGDTSEKTQDLLLLDVAPLSMGIETAGGVMTPLIKRNTTIPTKKSEIFSTY 429

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP7C_CRIGR (Heat shock cognate 71 kDa protein OS=Cricetulus griseus GN=HSPA8 PE=2 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 9.517e-67
Identity = 68/87 (78.16%), Postives = 76/87 (87.36%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCER 263
            RMVNHF+ EFKR +KKDIS N RA+RRLRTACERAKRTLSS+ Q +IEIDSLYEGIDFYT+ITRARFEELN DLFR  ++PVEK  R
Sbjct:  236 RMVNHFIAEFKRNDKKDISENKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALR 322

HSP 2 Score: 136.732 bits (343), Expect = 9.517e-67
Identity = 70/110 (63.64%), Postives = 79/110 (71.82%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            RDAK+DKS +HD+VLVGGSTRIPK+Q+LLQDFFNGKEL KSINPDE           ILSG+ +E VQD             ETAGGVMTVLI RNTTIPTK+ Q F+TY
Sbjct:  322 RDAKLDKSQIHDIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTY 431

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP71_SCHPO (Probable heat shock protein ssa1 OS=Schizosaccharomyces pombe GN=ssa1 PE=1 SV=2)

HSP 1 Score: 144.821 bits (364), Expect = 1.241e-66
Identity = 72/87 (82.76%), Postives = 78/87 (89.66%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCER 263
            R+VNHF QEFKRKNKKDI+GN RA+RRLRTACERAKRTLSS+AQ +IEIDSLYEGIDFYT+ITRARFEEL  DLFR  MEPVEK  R
Sbjct:  234 RLVNHFAQEFKRKNKKDITGNARAVRRLRTACERAKRTLSSSAQASIEIDSLYEGIDFYTSITRARFEELCADLFRNTMEPVEKVLR 320

HSP 2 Score: 129.798 bits (325), Expect = 1.241e-66
Identity = 66/110 (60.00%), Postives = 78/110 (70.91%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            RD+K+DKSSV+++VLVGGSTRIP++Q+L+ DFFNGKE CKSINPDE           IL G+ +EK QD             ETAGGVMT LI RNTTIPTKK +VFSTY
Sbjct:  320 RDSKIDKSSVNEIVLVGGSTRIPRIQKLVSDFFNGKEPCKSINPDEAVAYGAAVQAAILVGDTSEKTQDLLLLDVAPLSLGIETAGGVMTPLIKRNTTIPTKKSEVFSTY 429

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP7C_ORYLA (Heat shock cognate 71 kDa protein OS=Oryzias latipes GN=hsc70 PE=2 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 1.618e-66
Identity = 67/87 (77.01%), Postives = 76/87 (87.36%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCER 263
            RMVNHF+ EFKRK KKDIS N RA+RRLR+ACERAKRTLSS+ Q +IEIDSLYEG+DFYT+ITRARFEELN DLFR  ++PVEK  R
Sbjct:  236 RMVNHFIAEFKRKYKKDISDNKRAVRRLRSACERAKRTLSSSTQASIEIDSLYEGVDFYTSITRARFEELNADLFRGTLDPVEKSLR 322

HSP 2 Score: 137.117 bits (344), Expect = 1.618e-66
Identity = 70/113 (61.95%), Postives = 80/113 (70.80%), Query Frame = 1

Query:  250 RNARDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            ++ RDAKMDK  +HD+VLVGGSTRIPK+Q+LLQDFFNGKEL KSINPDE           ILSG+ +E VQD             ETAGGVMTVLI RNTTIPTK+ Q F+TY
Sbjct:  319 KSLRDAKMDKGQIHDIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTY 431

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP7C_CHICK (Heat shock cognate 71 kDa protein OS=Gallus gallus GN=HSPA8 PE=1 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 1.618e-66
Identity = 67/87 (77.01%), Postives = 77/87 (88.51%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCER 263
            R+VNHF+ EFKRK+KKDIS N RA+RRLRTACERAKRTLSS+ Q +IEIDSLYEGIDFYT+ITRARFE+LN DLFR  ++PVEK  R
Sbjct:  236 RLVNHFIAEFKRKHKKDISENKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEKLNADLFRGTLDPVEKALR 322

HSP 2 Score: 136.732 bits (343), Expect = 1.618e-66
Identity = 70/110 (63.64%), Postives = 79/110 (71.82%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            RDAK+DKS +HD+VLVGGSTRIPK+Q+LLQDFFNGKEL KSINPDE           ILSG+ +E VQD             ETAGGVMTVLI RNTTIPTK+ Q F+TY
Sbjct:  322 RDAKLDKSQIHDIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTY 431

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP71_ORYLA (Heat shock 70 kDa protein 1 OS=Oryzias latipes PE=3 SV=1)

HSP 1 Score: 139.043 bits (349), Expect = 3.589e-66
Identity = 69/87 (79.31%), Postives = 78/87 (89.66%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCER 263
            RMVNHFV+EFKRK+KKDIS N RALRRLRTACERAKRTLSS++Q +IEIDSL+EGIDFYT++TRARFEEL  DLFR  +EPVEK  R
Sbjct:  238 RMVNHFVEEFKRKHKKDISQNKRALRRLRTACERAKRTLSSSSQASIEIDSLFEGIDFYTSVTRARFEELCSDLFRGTLEPVEKALR 324

HSP 2 Score: 134.035 bits (336), Expect = 3.589e-66
Identity = 70/110 (63.64%), Postives = 77/110 (70.00%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            RDAKMDK  +HDVVLVGGSTRIPK+Q+LLQDFFNG+EL KSINPDE           ILSG+ +  VQD             ETAGGVMT LI RNTTIPTK+ QVFSTY
Sbjct:  324 RDAKMDKGQIHDVVLVGGSTRIPKIQKLLQDFFNGRELNKSINPDEAVAYGAAVQAAILSGDTSGNVQDLLLLDVAPLSLGIETAGGVMTALIKRNTTIPTKQTQVFSTY 433

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HS7C1_DICDI (Heat shock cognate 70 kDa protein 1 OS=Dictyostelium discoideum GN=hspB PE=1 SV=2)

HSP 1 Score: 138.658 bits (348), Expect = 4.679e-66
Identity = 67/84 (79.76%), Postives = 77/84 (91.67%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            R+V+HFV EFKRK+KKDI GN RA+RRLRTACERAKRTLSS+AQ +IEIDSL+EGIDFYT+ITRARFEEL  DLFR C++PVEK
Sbjct:  234 RLVSHFVDEFKRKHKKDIMGNQRAVRRLRTACERAKRTLSSSAQASIEIDSLFEGIDFYTSITRARFEELCADLFRGCLDPVEK 317

HSP 2 Score: 134.035 bits (336), Expect = 4.679e-66
Identity = 69/110 (62.73%), Postives = 76/110 (69.09%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            +D+K+DK S+H++VLVGGSTRIPKVQQLLQ+FFNGKEL KSINPDE           ILS EG  KV D             ETAGGVMT LIPRNTTIP KK Q FSTY
Sbjct:  320 KDSKLDKKSIHEIVLVGGSTRIPKVQQLLQEFFNGKELNKSINPDEAVAYGAAVQAAILSNEGGAKVADLLLLDVAPLSMGLETAGGVMTTLIPRNTTIPCKKTQTFSTY 429

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP71_PUCGR (Heat shock protein HSS1 OS=Puccinia graminis GN=HSS1 PE=3 SV=1)

HSP 1 Score: 146.362 bits (368), Expect = 1.764e-65
Identity = 71/87 (81.61%), Postives = 79/87 (90.80%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCER 263
            R+VNHFVQEFKRK+KKD+S NPRALRRLRTACERAKRTLSS AQTTIEIDSL+EG+DFYT++TRARFEEL  DLFR  +EPVEK  R
Sbjct:  234 RLVNHFVQEFKRKHKKDLSSNPRALRRLRTACERAKRTLSSAAQTTIEIDSLFEGVDFYTSLTRARFEELCQDLFRSTLEPVEKVLR 320

HSP 2 Score: 124.405 bits (311), Expect = 1.764e-65
Identity = 62/110 (56.36%), Postives = 77/110 (70.00%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            RDAK+DK++VH++VLVGGSTRIP++ +L+ DFFNGKE  KSINPDE           IL+G+ +EK QD             ETAGGVMT LI RNTT+PTKK ++FSTY
Sbjct:  320 RDAKIDKAAVHEIVLVGGSTRIPRIVKLVSDFFNGKEPNKSINPDEAVAYGAAVQAAILTGDTSEKTQDLLLLDVSPLSLGIETAGGVMTPLIKRNTTVPTKKSEIFSTY 429

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP72_USTMA (Heat shock 70 kDa protein 2 OS=Ustilago maydis GN=UMS2 PE=3 SV=2)

HSP 1 Score: 140.198 bits (352), Expect = 3.000e-65
Identity = 71/87 (81.61%), Postives = 77/87 (88.51%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCER 263
            R+VNHFVQEFKRKNKKD++ N RALRRLRTACERAKRTLSS AQTTIEIDSL+EGIDFYT+ITRARFEEL  DLF   +EPVEK  R
Sbjct:  234 RLVNHFVQEFKRKNKKDLTTNARALRRLRTACERAKRTLSSAAQTTIEIDSLFEGIDFYTSITRARFEELCGDLFSHTIEPVEKVLR 320

HSP 2 Score: 129.798 bits (325), Expect = 3.000e-65
Identity = 66/110 (60.00%), Postives = 77/110 (70.00%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            RD+K+DK SVH++VLVGGSTRIPKVQ+LL DFFNG+EL KSINPDE           ILSG+ +EK QD             ETAGGV T LI RNTT+PTKK ++FSTY
Sbjct:  320 RDSKIDKGSVHEIVLVGGSTRIPKVQKLLTDFFNGRELNKSINPDEAVAYGAAVQAAILSGDTSEKTQDLLLLDVAPLSMGIETAGGVFTPLIKRNTTVPTKKSEIFSTY 429

The following BLAST results are available for this feature:

BLAST of CB417297 vs. ExPASy Swiss-Prot

BLAST of CB417297 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
HSP7C_DANRE	4.292e-67	79.31	Heat shock cognate 71 kDa protein OS=Danio rerio G...	[more]
HSP72_SCHPO	9.517e-67	81.61	Probable heat shock protein ssa2 OS=Schizosaccharo...	[more]
HSP7C_CRIGR	9.517e-67	78.16	Heat shock cognate 71 kDa protein OS=Cricetulus gr...	[more]
HSP71_SCHPO	1.241e-66	82.76	Probable heat shock protein ssa1 OS=Schizosaccharo...	[more]
HSP7C_ORYLA	1.618e-66	77.01	Heat shock cognate 71 kDa protein OS=Oryzias latip...	[more]
HSP7C_CHICK	1.618e-66	77.01	Heat shock cognate 71 kDa protein OS=Gallus gallus...	[more]
HSP71_ORYLA	3.589e-66	79.31	Heat shock 70 kDa protein 1 OS=Oryzias latipes PE=...	[more]
HS7C1_DICDI	4.679e-66	79.76	Heat shock cognate 70 kDa protein 1 OS=Dictyosteli...	[more]
HSP71_PUCGR	1.764e-65	81.61	Heat shock protein HSS1 OS=Puccinia graminis GN=HS...	[more]
HSP72_USTMA	3.000e-65	81.61	Heat shock 70 kDa protein 2 OS=Ustilago maydis GN=...	[more]

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Properties


Property Name	Value
Genbank description	EST0370 Mature peel (flavedo) cDNA subtraction library Citrus sinensis cDNA clone 6P015 similar to 70 kDa heat shock protein (acc# AAL24367), mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CB417297 ID=CB417297; Name=CB417297; organism=Citrus sinensis; type=EST; length=590bp
ACAGAATGGTGAACCACTTTGTTCAAGAGTTCAAGAGGAAAAACAAGAAG
GATATTAGTGGAAACCCCAGAGCGTTGAGGAGGTTGAGAACTGCCTGTGA
AAGGGCTAAGAGGACCCTGTCATCAACCGCACAGACTACAATTGAGATTG
ATTCTTTATATGAGGGTATTGATTTCTACACAACCATTACAAGGGCTAGA
TTCGAAGAGTTGAACATGGATTTGTTCAGGAAGTGTATGGAGCCAGTGGA
GAAATGCGAGAGATGCTAAGATGGACAAGAGCAGTGTCCATGATGTGGTT
CTTGTTGGTGGGTCTACTAGGATCCCAAAAGTCCAGCAACTGTTGCAGGA
CTTCTTCAATGGAAAAGAGCTATGCAAGAGCATCAACCCTGATGAAGCGG
TTGCCTACGGTGCTGCTGTGCAGGCTGCAATCTTGAGTGGTGAGGGTAAT
GAGAAGGTTCAGGACCTCTTATTGCTTGACGTCACCCCTCTATCGCTGGG
TTTGGAGACTGCTGGTGGAGTCATGACTGTGTTGATTCCAAGGAACACCA
CTATTCCAACCAAGAAGGAGCAGGTGTTCTCCACATACTC