CB417297 | Citrus Genome Database

Overview


Name	CB417297
Unique Name	CB417297
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	590

Libraries


Library Name	Type
Mature peel (flavedo) cDNA subtraction library	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig13846	contig	Csv1_Contig13846:800..1391.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP7A_DROME (Heat shock 70 kDa protein cognate 1 OS=Drosophila melanogaster GN=Hsc70-1 PE=1 SV=1)

HSP 1 Score: 135.961 bits (341), Expect = 3.000e-65
Identity = 66/87 (75.86%), Postives = 76/87 (87.36%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCER 263
            R+VNHFVQEF+RK+KKD+  N RALRRLRTACERAKRTLSS+ Q +IEIDSL+EG+DFYT++TRARFEELN DLFR  MEPV K  R
Sbjct:  236 RLVNHFVQEFQRKHKKDLGQNKRALRRLRTACERAKRTLSSSTQASIEIDSLFEGVDFYTSVTRARFEELNGDLFRGTMEPVAKALR 322

HSP 2 Score: 134.035 bits (336), Expect = 3.000e-65
Identity = 69/110 (62.73%), Postives = 77/110 (70.00%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            RDAKMDK  +HD+VLVGGSTRIPKVQ+LLQDFFNGKEL KSINPDE           IL G+ +E VQD             ETAGGVMT LI RNTTIPTK+ Q+F+TY
Sbjct:  322 RDAKMDKGQIHDIVLVGGSTRIPKVQRLLQDFFNGKELNKSINPDEAVAYGAAVQAAILHGDKSEAVQDLLLLDVTPLSLGIETAGGVMTTLIKRNTTIPTKQTQIFTTY 431

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP70_BRUMA (Heat shock 70 kDa protein OS=Brugia malayi GN=HSP70 PE=3 SV=1)

HSP 1 Score: 138.658 bits (348), Expect = 6.651e-65
Identity = 68/87 (78.16%), Postives = 76/87 (87.36%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCER 263
            RMVNHFV EFKR +KKD++ NPRALRRLRTACERAKRTLSS++Q +IEIDSL+EGIDFYT ITRARFEEL  DLFR  M+PVEK  R
Sbjct:  235 RMVNHFVAEFKRNDKKDLASNPRALRRLRTACERAKRTLSSSSQASIEIDSLFEGIDFYTNITRARFEELCADLFRSTMDPVEKALR 321

HSP 2 Score: 130.183 bits (326), Expect = 6.651e-65
Identity = 68/110 (61.82%), Postives = 76/110 (69.09%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            RDAKMDK+ VHD+VLVGGSTRIPKVQ+LL DFF+GKEL KSINPDE           ILSG+ +E VQD             ETAGGVMT LI RNTTIPTK  + F+TY
Sbjct:  321 RDAKMDKAQVHDIVLVGGSTRIPKVQKLLSDFFSGKELNKSINPDEAVAYGAAVQAAILSGDKSEAVQDLLLLDVAPLSLGIETAGGVMTALIKRNTTIPTKTSETFTTY 430

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP70_ONCTS (Heat shock 70 kDa protein OS=Oncorhynchus tschawytscha GN=hsp70 PE=2 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 6.651e-65
Identity = 67/87 (77.01%), Postives = 78/87 (89.66%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCER 263
            R+V+HFV+EFKRK+KKDIS N RALRRLRTACERAKRTLSS++Q +IEIDSL+EGIDFYT+ITRARFEE+  DLFR  +EPVEK  R
Sbjct:  238 RLVSHFVEEFKRKHKKDISQNKRALRRLRTACERAKRTLSSSSQASIEIDSLFEGIDFYTSITRARFEEMCSDLFRGTLEPVEKALR 324

HSP 2 Score: 133.265 bits (334), Expect = 6.651e-65
Identity = 69/110 (62.73%), Postives = 78/110 (70.91%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            RDAKMDK+ +HDVVLVGGSTRIPKVQ+LLQDFFNG+EL KSINPDE           ILSG+ +E VQD             ETAGGVMT LI RNTTIP+K+ Q F+TY
Sbjct:  324 RDAKMDKAQIHDVVLVGGSTRIPKVQKLLQDFFNGRELNKSINPDEAVAYGAAIQAAILSGDKSENVQDLLLLDVAPLSLGIETAGGVMTALIKRNTTIPSKQTQTFTTY 433

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP7A_CAEEL (Heat shock 70 kDa protein A OS=Caenorhabditis elegans GN=hsp-1 PE=1 SV=2)

HSP 1 Score: 138.272 bits (347), Expect = 6.651e-65
Identity = 68/87 (78.16%), Postives = 76/87 (87.36%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCER 263
            RMVNHF  EFKRK+KKD++ NPRALRRLRTACERAKRTLSS++Q +IEIDSL+EGIDFYT ITRARFEEL  DLFR  M+PVEK  R
Sbjct:  237 RMVNHFCAEFKRKHKKDLASNPRALRRLRTACERAKRTLSSSSQASIEIDSLFEGIDFYTNITRARFEELCADLFRSTMDPVEKSLR 323

HSP 2 Score: 130.568 bits (327), Expect = 6.651e-65
Identity = 69/113 (61.06%), Postives = 77/113 (68.14%), Query Frame = 1

Query:  250 RNARDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            ++ RDAKMDKS VHD+VLVGGSTRIPKVQ+LL D F+GKEL KSINPDE           ILSG+ +E VQD             ETAGGVMT LI RNTTIPTK  Q F+TY
Sbjct:  320 KSLRDAKMDKSQVHDIVLVGGSTRIPKVQKLLSDLFSGKELNKSINPDEAVAYGAAVQAAILSGDKSEAVQDLLLLDVAPLSLGIETAGGVMTALIKRNTTIPTKTAQTFTTY 432

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HS71L_RAT (Heat shock 70 kDa protein 1-like OS=Rattus norvegicus GN=Hspa1l PE=2 SV=2)

HSP 1 Score: 134.806 bits (338), Expect = 6.651e-65
Identity = 67/87 (77.01%), Postives = 76/87 (87.36%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCER 263
            R+V+HFV+EFKRK+KKDIS N RA+RRLRTACERAKRTLSS+ Q  +EIDSLYEGIDFYT+ITRARFEEL  DLFR  +EPVEK  R
Sbjct:  238 RLVSHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQANLEIDSLYEGIDFYTSITRARFEELCADLFRGTLEPVEKSLR 324

HSP 2 Score: 134.035 bits (336), Expect = 6.651e-65
Identity = 67/113 (59.29%), Postives = 82/113 (72.57%), Query Frame = 1

Query:  250 RNARDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            ++ RDAKMDK+ +HD+VLVGGSTRIPKVQ+LLQD+FNG++L KSINPDE           IL G+ +EKVQD             ETAGGVMTVLI RN+TIPTK+ Q+F+TY
Sbjct:  321 KSLRDAKMDKAKIHDIVLVGGSTRIPKVQKLLQDYFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSEKVQDLLLLDVAPLSLGLETAGGVMTVLIKRNSTIPTKQTQIFTTY 433

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HS71L_MOUSE (Heat shock 70 kDa protein 1-like OS=Mus musculus GN=Hspa1l PE=2 SV=4)

HSP 1 Score: 134.806 bits (338), Expect = 6.651e-65
Identity = 67/87 (77.01%), Postives = 76/87 (87.36%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCER 263
            R+V+HFV+EFKRK+KKDIS N RA+RRLRTACERAKRTLSS+ Q  +EIDSLYEGIDFYT+ITRARFEEL  DLFR  +EPVEK  R
Sbjct:  238 RLVSHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQANLEIDSLYEGIDFYTSITRARFEELCADLFRGTLEPVEKSLR 324

HSP 2 Score: 134.035 bits (336), Expect = 6.651e-65
Identity = 67/113 (59.29%), Postives = 82/113 (72.57%), Query Frame = 1

Query:  250 RNARDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            ++ RDAKMDK+ +HD+VLVGGSTRIPKVQ+LLQD+FNG++L KSINPDE           IL G+ +EKVQD             ETAGGVMTVLI RN+TIPTK+ Q+F+TY
Sbjct:  321 KSLRDAKMDKAKIHDIVLVGGSTRIPKVQKLLQDYFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSEKVQDLLLLDVAPLSLGLETAGGVMTVLIKRNSTIPTKQTQIFTTY 433

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HS7C2_DICDI (Heat shock cognate 70 kDa protein 2 OS=Dictyostelium discoideum GN=hspE-1 PE=1 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 6.652e-65
Identity = 66/84 (78.57%), Postives = 76/84 (90.48%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            R+VNHFV EFKRK+KKD+  N RALRRLRTACERAKRTLSS+AQ +IEIDSL+EGIDFYT+ITRARFEEL  DLFR C++PV+K
Sbjct:  235 RLVNHFVDEFKRKHKKDLMTNQRALRRLRTACERAKRTLSSSAQASIEIDSLFEGIDFYTSITRARFEELCADLFRGCLDPVDK 318

HSP 2 Score: 132.109 bits (331), Expect = 6.652e-65
Identity = 68/110 (61.82%), Postives = 75/110 (68.18%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            +D+K+DK ++H++VLVGGSTRIPKVQQLLQD FNGKEL KSINPDE           ILS EG  KV D             ETAGGVMT LIPRNTTIP KK Q FSTY
Sbjct:  321 KDSKLDKKTIHEIVLVGGSTRIPKVQQLLQDHFNGKELNKSINPDEAVAYGAAVQAAILSNEGGAKVADILLLDVAPLSMGLETAGGVMTTLIPRNTTIPCKKNQTFSTY 430

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP72_PICAN (Heat shock protein 70 2 OS=Pichia angusta GN=HSA2 PE=3 SV=2)

HSP 1 Score: 140.969 bits (354), Expect = 8.673e-65
Identity = 68/84 (80.95%), Postives = 76/84 (90.48%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            R+VNHF+ EFKRKNKKDI GN RALRRLRTACERAK TLSS+AQT++EIDSLYEGIDFYT+ITRARFEEL  DLFR  ++PVEK
Sbjct:  234 RLVNHFINEFKRKNKKDICGNQRALRRLRTACERAKTTLSSSAQTSLEIDSLYEGIDFYTSITRARFEELCQDLFRSTLDPVEK 317

HSP 2 Score: 127.487 bits (319), Expect = 8.673e-65
Identity = 65/110 (59.09%), Postives = 77/110 (70.00%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            +DAK+DKS V+++VLVGGSTRIPKVQ+L+ DFFNGKE  KSINPDE           IL+G+ + K QD             ETAGGVMT LIPRNTTIPTKK ++FSTY
Sbjct:  320 KDAKLDKSQVNEIVLVGGSTRIPKVQKLVSDFFNGKEPNKSINPDEAVAYGAAVQAAILTGDTSSKTQDLLLLDVAPLSLGIETAGGVMTKLIPRNTTIPTKKSEIFSTY 429

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HS71L_PIG (Heat shock 70 kDa protein 1-like OS=Sus scrofa GN=HSPA1L PE=2 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 8.673e-65
Identity = 67/87 (77.01%), Postives = 76/87 (87.36%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCER 263
            R+V+HFV+EFKRK+KKDIS N RA+RRLRTACERAKRTLSS+ Q  +EIDSLYEGIDFYT+ITRARFEEL  DLFR  +EPVEK  R
Sbjct:  238 RLVSHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQANLEIDSLYEGIDFYTSITRARFEELCADLFRGTLEPVEKALR 324

HSP 2 Score: 133.265 bits (334), Expect = 8.673e-65
Identity = 67/110 (60.91%), Postives = 80/110 (72.73%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            RDAKMDK+ +HD+VLVGGSTRIPKVQ+LLQD+FNG++L KSINPDE           IL G+ +EKVQD             ETAGGVMTVLI RN+TIPTK+ Q+F+TY
Sbjct:  324 RDAKMDKAKIHDIVLVGGSTRIPKVQRLLQDYFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSEKVQDLLLLDVAPLSLGLETAGGVMTVLIKRNSTIPTKQTQIFTTY 433

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HS71L_BOVIN (Heat shock 70 kDa protein 1-like OS=Bos taurus GN=HSPA1L PE=3 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 8.673e-65
Identity = 67/87 (77.01%), Postives = 76/87 (87.36%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCER 263
            R+V+HFV+EFKRK+KKDIS N RA+RRLRTACERAKRTLSS+ Q  +EIDSLYEGIDFYT+ITRARFEEL  DLFR  +EPVEK  R
Sbjct:  238 RLVSHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQANLEIDSLYEGIDFYTSITRARFEELCADLFRGTLEPVEKALR 324

HSP 2 Score: 133.265 bits (334), Expect = 8.673e-65
Identity = 67/110 (60.91%), Postives = 80/110 (72.73%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            RDAKMDK+ +HD+VLVGGSTRIPKVQ+LLQD+FNG++L KSINPDE           IL G+ +EKVQD             ETAGGVMTVLI RN+TIPTK+ Q+F+TY
Sbjct:  324 RDAKMDKAKIHDIVLVGGSTRIPKVQRLLQDYFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSEKVQDLLLLDVAPLSLGLETAGGVMTVLIKRNSTIPTKQTQIFTTY 433

The following BLAST results are available for this feature:

BLAST of CB417297 vs. ExPASy Swiss-Prot

BLAST of CB417297 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
HSP7A_DROME	3.000e-65	75.86	Heat shock 70 kDa protein cognate 1 OS=Drosophila ...	[more]
HSP70_BRUMA	6.651e-65	78.16	Heat shock 70 kDa protein OS=Brugia malayi GN=HSP7...	[more]
HSP70_ONCTS	6.651e-65	77.01	Heat shock 70 kDa protein OS=Oncorhynchus tschawyt...	[more]
HSP7A_CAEEL	6.651e-65	78.16	Heat shock 70 kDa protein A OS=Caenorhabditis eleg...	[more]
HS71L_RAT	6.651e-65	77.01	Heat shock 70 kDa protein 1-like OS=Rattus norvegi...	[more]
HS71L_MOUSE	6.651e-65	77.01	Heat shock 70 kDa protein 1-like OS=Mus musculus G...	[more]
HS7C2_DICDI	6.652e-65	78.57	Heat shock cognate 70 kDa protein 2 OS=Dictyosteli...	[more]
HSP72_PICAN	8.673e-65	80.95	Heat shock protein 70 2 OS=Pichia angusta GN=HSA2 ...	[more]
HS71L_PIG	8.673e-65	77.01	Heat shock 70 kDa protein 1-like OS=Sus scrofa GN=...	[more]
HS71L_BOVIN	8.673e-65	77.01	Heat shock 70 kDa protein 1-like OS=Bos taurus GN=...	[more]

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Properties


Property Name	Value
Genbank description	EST0370 Mature peel (flavedo) cDNA subtraction library Citrus sinensis cDNA clone 6P015 similar to 70 kDa heat shock protein (acc# AAL24367), mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CB417297 ID=CB417297; Name=CB417297; organism=Citrus sinensis; type=EST; length=590bp
ACAGAATGGTGAACCACTTTGTTCAAGAGTTCAAGAGGAAAAACAAGAAG
GATATTAGTGGAAACCCCAGAGCGTTGAGGAGGTTGAGAACTGCCTGTGA
AAGGGCTAAGAGGACCCTGTCATCAACCGCACAGACTACAATTGAGATTG
ATTCTTTATATGAGGGTATTGATTTCTACACAACCATTACAAGGGCTAGA
TTCGAAGAGTTGAACATGGATTTGTTCAGGAAGTGTATGGAGCCAGTGGA
GAAATGCGAGAGATGCTAAGATGGACAAGAGCAGTGTCCATGATGTGGTT
CTTGTTGGTGGGTCTACTAGGATCCCAAAAGTCCAGCAACTGTTGCAGGA
CTTCTTCAATGGAAAAGAGCTATGCAAGAGCATCAACCCTGATGAAGCGG
TTGCCTACGGTGCTGCTGTGCAGGCTGCAATCTTGAGTGGTGAGGGTAAT
GAGAAGGTTCAGGACCTCTTATTGCTTGACGTCACCCCTCTATCGCTGGG
TTTGGAGACTGCTGGTGGAGTCATGACTGTGTTGATTCCAAGGAACACCA
CTATTCCAACCAAGAAGGAGCAGGTGTTCTCCACATACTC