CB417297 | Citrus Genome Database

Overview


Name	CB417297
Unique Name	CB417297
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	590

Libraries


Library Name	Type
Mature peel (flavedo) cDNA subtraction library	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_SALEP (Chaperone protein dnaK OS=Salmonella enteritidis PT4 (strain P125109) GN=dnaK PE=2 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 4.808e-26
Identity = 49/109 (44.95%), Postives = 64/109 (58.72%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +  S ++DV+LVGG TR+P VQ+ + +FF GKE  K +NPDE           +L+G+    V+D             ET GGVMT LI +NTTIPTK  QVFST
Sbjct:  325 QDAGLSVSDINDVILVGGQTRMPMVQKKVAEFF-GKEPRKDVNPDEAVAIGAAVQGGVLTGD----VKDVLLLDVTPLSLGIETMGGVMTPLITKNTTIPTKHSQVFST 428

HSP 2 Score: 53.5286 bits (127), Expect = 4.808e-26
Identity = 29/87 (33.33%), Postives = 48/87 (55.17%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGI----DFYTTITRARFEELNMDLFRKCMEPVE 251
            R++N+ V EFK+    D+  +P A++RL+ A E+AK  LSS  QT + +  +             +TRA+ E L  DL  + +EP++
Sbjct:  235 RLINYLVDEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSAQQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLK 321

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_SALDC (Chaperone protein dnaK OS=Salmonella dublin (strain CT_02021853) GN=dnaK PE=2 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 4.808e-26
Identity = 49/109 (44.95%), Postives = 64/109 (58.72%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +  S ++DV+LVGG TR+P VQ+ + +FF GKE  K +NPDE           +L+G+    V+D             ET GGVMT LI +NTTIPTK  QVFST
Sbjct:  325 QDAGLSVSDINDVILVGGQTRMPMVQKKVAEFF-GKEPRKDVNPDEAVAIGAAVQGGVLTGD----VKDVLLLDVTPLSLGIETMGGVMTPLITKNTTIPTKHSQVFST 428

HSP 2 Score: 53.5286 bits (127), Expect = 4.808e-26
Identity = 29/87 (33.33%), Postives = 48/87 (55.17%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGI----DFYTTITRARFEELNMDLFRKCMEPVE 251
            R++N+ V EFK+    D+  +P A++RL+ A E+AK  LSS  QT + +  +             +TRA+ E L  DL  + +EP++
Sbjct:  235 RLINYLVDEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSAQQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLK 321

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_SALCH (Chaperone protein dnaK OS=Salmonella choleraesuis GN=dnaK PE=2 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 4.808e-26
Identity = 49/109 (44.95%), Postives = 64/109 (58.72%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +  S ++DV+LVGG TR+P VQ+ + +FF GKE  K +NPDE           +L+G+    V+D             ET GGVMT LI +NTTIPTK  QVFST
Sbjct:  325 QDAGLSVSDINDVILVGGQTRMPMVQKKVAEFF-GKEPRKDVNPDEAVAIGAAVQGGVLTGD----VKDVLLLDVTPLSLGIETMGGVMTPLITKNTTIPTKHSQVFST 428

HSP 2 Score: 53.5286 bits (127), Expect = 4.808e-26
Identity = 29/87 (33.33%), Postives = 48/87 (55.17%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGI----DFYTTITRARFEELNMDLFRKCMEPVE 251
            R++N+ V EFK+    D+  +P A++RL+ A E+AK  LSS  QT + +  +             +TRA+ E L  DL  + +EP++
Sbjct:  235 RLINYLVDEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSAQQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLK 321

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_SALAR (Chaperone protein dnaK OS=Salmonella arizonae (strain ATCC BAA-731 / CDC346-86 / RSK2980) GN=dnaK PE=2 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 4.808e-26
Identity = 49/109 (44.95%), Postives = 64/109 (58.72%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +  S ++DV+LVGG TR+P VQ+ + +FF GKE  K +NPDE           +L+G+    V+D             ET GGVMT LI +NTTIPTK  QVFST
Sbjct:  325 QDAGLSVSDINDVILVGGQTRMPMVQKKVAEFF-GKEPRKDVNPDEAVAIGAAVQGGVLTGD----VKDVLLLDVTPLSLGIETMGGVMTPLISKNTTIPTKHSQVFST 428

HSP 2 Score: 53.5286 bits (127), Expect = 4.808e-26
Identity = 29/87 (33.33%), Postives = 48/87 (55.17%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGI----DFYTTITRARFEELNMDLFRKCMEPVE 251
            R++N+ V EFK+    D+  +P A++RL+ A E+AK  LSS  QT + +  +             +TRA+ E L  DL  + +EP++
Sbjct:  235 RLINYLVDEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSAQQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLK 321

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_SALA4 (Chaperone protein dnaK OS=Salmonella agona (strain SL483) GN=dnaK PE=2 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 4.808e-26
Identity = 49/109 (44.95%), Postives = 64/109 (58.72%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +  S ++DV+LVGG TR+P VQ+ + +FF GKE  K +NPDE           +L+G+    V+D             ET GGVMT LI +NTTIPTK  QVFST
Sbjct:  325 QDAGLSVSDINDVILVGGQTRMPMVQKKVAEFF-GKEPRKDVNPDEAVAIGAAVQGGVLTGD----VKDVLLLDVTPLSLGIETMGGVMTPLITKNTTIPTKHSQVFST 428

HSP 2 Score: 53.5286 bits (127), Expect = 4.808e-26
Identity = 29/87 (33.33%), Postives = 48/87 (55.17%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGI----DFYTTITRARFEELNMDLFRKCMEPVE 251
            R++N+ V EFK+    D+  +P A++RL+ A E+AK  LSS  QT + +  +             +TRA+ E L  DL  + +EP++
Sbjct:  235 RLINYLVDEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSAQQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLK 321

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_PARDP (Chaperone protein dnaK OS=Paracoccus denitrificans (strain Pd 1222) GN=dnaK PE=2 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 4.808e-26
Identity = 49/109 (44.95%), Postives = 61/109 (55.96%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA M K  + ++VLVGG TR+PKV + +  FF GKE  K +NPDE           +L G+    V+D             ET GGV T LI RNTTIPTKK Q+FST
Sbjct:  321 KDAGMSKGDIDEIVLVGGMTRMPKVVEEVTAFF-GKEPHKGVNPDEVVALGAAIQAGVLQGD----VKDVVLLDVTPLSLGIETLGGVFTRLIDRNTTIPTKKSQIFST 424

HSP 2 Score: 55.0694 bits (131), Expect = 4.808e-26
Identity = 33/91 (36.26%), Postives = 55/91 (60.44%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGID--------FYTTITRARFEELNMDLFRKCMEPVE 251
            R+VN+   EFK+++  D++ +  AL+RL+ A E+AK  LSS++QT  EI+  +  +D            +TRA+ E L  DL ++ M+PV+
Sbjct:  229 RIVNYLADEFKKEHGVDLTKDKMALQRLKEAAEKAKIELSSSSQT--EINQPFISMDKDSGTPLHMVMKLTRAKLESLVGDLIKRTMKPVQ 317

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_SHEPA (Chaperone protein dnaK OS=Shewanella pealeana (strain ATCC 700345 / ANG-SQ1) GN=dnaK PE=2 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 4.808e-26
Identity = 47/108 (43.52%), Postives = 62/108 (57.41%), Query Frame = 1

Query:  262 DAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            DA +  S +++V+LVGG TR+PKV++ +  FF GKEL + +NPDE           +LSG+    V+D             ET G VMT LI +NTTIPTK  Q FST
Sbjct:  325 DADLSVSDINEVILVGGQTRMPKVREEVSAFF-GKELRQDVNPDEAVAIGAAVQAGVLSGD----VKDVLLLDVTPLSLGIETMGSVMTKLIEKNTTIPTKASQTFST 427

HSP 2 Score: 56.9954 bits (136), Expect = 4.808e-26
Identity = 30/87 (34.48%), Postives = 49/87 (56.32%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGI----DFYTTITRARFEELNMDLFRKCMEPVE 251
            RM+ +   EFK++   D+  +P A++RL+ A E+AK  LSST QT + +  +             ITRA+ E L  DL ++ +EP++
Sbjct:  234 RMIKYLADEFKKEQGLDLRNDPLAMQRLKEAAEKAKIELSSTTQTEVNLPYITADATGPKHLVVKITRAKLESLVEDLIKRSLEPLK 320

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_SHEHH (Chaperone protein dnaK OS=Shewanella halifaxensis (strain HAW-EB4) GN=dnaK PE=2 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 4.808e-26
Identity = 47/108 (43.52%), Postives = 62/108 (57.41%), Query Frame = 1

Query:  262 DAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            DA +  S +++V+LVGG TR+PKV++ +  FF GKEL + +NPDE           +LSG+    V+D             ET G VMT LI +NTTIPTK  Q FST
Sbjct:  325 DADLSVSDINEVILVGGQTRMPKVREEVSAFF-GKELRQDVNPDEAVAIGAAVQAGVLSGD----VKDVLLLDVTPLSLGIETMGSVMTKLIEKNTTIPTKASQTFST 427

HSP 2 Score: 56.9954 bits (136), Expect = 4.808e-26
Identity = 30/87 (34.48%), Postives = 49/87 (56.32%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGI----DFYTTITRARFEELNMDLFRKCMEPVE 251
            RM+ +   EFK++   D+  +P A++RL+ A E+AK  LSST QT + +  +             ITRA+ E L  DL ++ +EP++
Sbjct:  234 RMIKYLADEFKKEQGLDLRNDPLAMQRLKEAAEKAKIELSSTTQTEVNLPYITADATGPKHLVVKITRAKLESLVEDLIKRSLEPLK 320

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_BACTN (Chaperone protein dnaK OS=Bacteroides thetaiotaomicron GN=dnaK PE=2 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 4.808e-26
Identity = 45/108 (41.67%), Postives = 60/108 (55.56%), Query Frame = 1

Query:  262 DAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            DA +  S + +V+LVGGS+RIP VQ+L++DFF GK   K +NPDE           +L+ E    ++              ET GGVMT LI  NTTIP +K + FST
Sbjct:  320 DAGLSNSDIDEVILVGGSSRIPAVQKLVEDFF-GKTPSKGVNPDEVVAVGAAVQGAVLTDE----IKGVVLLDVTPLSMGIETLGGVMTKLIDANTTIPARKSETFST 422

HSP 2 Score: 60.077 bits (144), Expect = 4.808e-26
Identity = 34/87 (39.08%), Postives = 51/87 (58.62%), Query Frame = 3

Query:    6 MVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL--YEGI--DFYTTITRARFEELNMDLFRKCMEPVEK 254
            ++N  VQEFK     D++ +P AL+RL+ A E+AK  LSS+  T I +  +    G+      T+TRA+FE L   L + C+EP +K
Sbjct:  230 IINWLVQEFKNDEGADLTQDPMALQRLKEAAEKAKIELSSSTSTEINLPYIMPVNGVPKHLVKTLTRAKFESLAHGLIQACLEPCKK 316

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_HAEDU (Chaperone protein dnaK OS=Haemophilus ducreyi GN=dnaK PE=2 SV=2)

HSP 1 Score: 87.4261 bits (215), Expect = 4.808e-26
Identity = 51/108 (47.22%), Postives = 63/108 (58.33%), Query Frame = 1

Query:  262 DAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            DA +  S ++DV+LVGG TR+P VQ+ + DFF GKE  K +NPDE           +L+G+    V D             ET GGVMT LI +NTTIPTKK QVFST
Sbjct:  325 DAGLSVSEINDVILVGGQTRMPLVQKKVADFF-GKEPRKDVNPDEAVAIGAAVQGGVLAGD----VTDVLLLDVTPLSLGIETMGGVMTSLIEKNTTIPTKKSQVFST 427

HSP 2 Score: 51.2174 bits (121), Expect = 4.808e-26
Identity = 26/87 (29.89%), Postives = 50/87 (57.47%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGI----DFYTTITRARFEELNMDLFRKCMEPVE 251
            R++N+ V+EF+++   D+  +P A++R++ A E+AK  LSS  +T + +  +             +TRA+ E L  DL  + +EP++
Sbjct:  234 RVINYLVEEFQKEQGVDLRNDPMAMQRVKEAAEKAKIELSSAQETEVNLPYITADATGPKHLNIKVTRAKLEALVEDLVSRSLEPLK 320

The following BLAST results are available for this feature:

BLAST of CB417297 vs. ExPASy Swiss-Prot

BLAST of CB417297 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
DNAK_SALEP	4.808e-26	44.95	Chaperone protein dnaK OS=Salmonella enteritidis P...	[more]
DNAK_SALDC	4.808e-26	44.95	Chaperone protein dnaK OS=Salmonella dublin (strai...	[more]
DNAK_SALCH	4.808e-26	44.95	Chaperone protein dnaK OS=Salmonella choleraesuis ...	[more]
DNAK_SALAR	4.808e-26	44.95	Chaperone protein dnaK OS=Salmonella arizonae (str...	[more]
DNAK_SALA4	4.808e-26	44.95	Chaperone protein dnaK OS=Salmonella agona (strain...	[more]
DNAK_PARDP	4.808e-26	44.95	Chaperone protein dnaK OS=Paracoccus denitrificans...	[more]
DNAK_SHEPA	4.808e-26	43.52	Chaperone protein dnaK OS=Shewanella pealeana (str...	[more]
DNAK_SHEHH	4.808e-26	43.52	Chaperone protein dnaK OS=Shewanella halifaxensis ...	[more]
DNAK_BACTN	4.808e-26	41.67	Chaperone protein dnaK OS=Bacteroides thetaiotaomi...	[more]
DNAK_HAEDU	4.808e-26	47.22	Chaperone protein dnaK OS=Haemophilus ducreyi GN=d...	[more]

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Properties


Property Name	Value
Genbank description	EST0370 Mature peel (flavedo) cDNA subtraction library Citrus sinensis cDNA clone 6P015 similar to 70 kDa heat shock protein (acc# AAL24367), mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CB417297 ID=CB417297; Name=CB417297; organism=Citrus sinensis; type=EST; length=590bp
ACAGAATGGTGAACCACTTTGTTCAAGAGTTCAAGAGGAAAAACAAGAAG
GATATTAGTGGAAACCCCAGAGCGTTGAGGAGGTTGAGAACTGCCTGTGA
AAGGGCTAAGAGGACCCTGTCATCAACCGCACAGACTACAATTGAGATTG
ATTCTTTATATGAGGGTATTGATTTCTACACAACCATTACAAGGGCTAGA
TTCGAAGAGTTGAACATGGATTTGTTCAGGAAGTGTATGGAGCCAGTGGA
GAAATGCGAGAGATGCTAAGATGGACAAGAGCAGTGTCCATGATGTGGTT
CTTGTTGGTGGGTCTACTAGGATCCCAAAAGTCCAGCAACTGTTGCAGGA
CTTCTTCAATGGAAAAGAGCTATGCAAGAGCATCAACCCTGATGAAGCGG
TTGCCTACGGTGCTGCTGTGCAGGCTGCAATCTTGAGTGGTGAGGGTAAT
GAGAAGGTTCAGGACCTCTTATTGCTTGACGTCACCCCTCTATCGCTGGG
TTTGGAGACTGCTGGTGGAGTCATGACTGTGTTGATTCCAAGGAACACCA
CTATTCCAACCAAGAAGGAGCAGGTGTTCTCCACATACTC