CB417297

Overview
NameCB417297
Unique NameCB417297
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length590
Libraries
Library NameType
Mature peel (flavedo) cDNA subtraction librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_RHOS7 (Chaperone protein dnaK OS=Rhodopseudomonas sp. (strain No.7) GN=dnaK PE=2 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 4.808e-26
Identity = 48/109 (44.04%), Postives = 62/109 (56.88%), Query Frame = 1 
Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +    + +VVLVGG TR+PKVQ++++  F GKE  K +NPDE           +L G+    V+D             ET GGV T +I RNTTIPTKK QVFST
Sbjct:  320 KDAGLTAGEISEVVLVGGMTRMPKVQEVVKQLF-GKEPHKGVNPDEVVAIGAAIQAGVLQGD----VKDVLLLDVTPLSLGIETLGGVFTRIIDRNTTIPTKKSQVFST 423

HSP 2 Score: 55.4546 bits (132), Expect = 4.808e-26
Identity = 35/88 (39.77%), Postives = 51/88 (57.95%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL---YEGIDFYT-TITRARFEELNMDLFRKCMEPVEK 254
            R+VN+   EF+++   D+  +  AL+RL+ A E+AK  LSST QT I +  +     G    T  +TRA+FE L  DL +K +EP  K
Sbjct:  230 RLVNYLADEFQKEQGIDLRKDKLALQRLKEAAEKAKIELSSTTQTEINLPFITADQSGPKHLTMKLTRAKFEALVDDLVQKTIEPCRK 317
[Close]
BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_RHOPT (Chaperone protein dnaK OS=Rhodopseudomonas palustris (strain TIE-1) GN=dnaK PE=2 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 4.808e-26
Identity = 48/109 (44.04%), Postives = 62/109 (56.88%), Query Frame = 1 
Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +    + +VVLVGG TR+PKVQ++++  F GKE  K +NPDE           +L G+    V+D             ET GGV T +I RNTTIPTKK QVFST
Sbjct:  320 KDAGLTAGEISEVVLVGGMTRMPKVQEVVKQLF-GKEPHKGVNPDEVVAIGAAIQAGVLQGD----VKDVLLLDVTPLSLGIETLGGVFTRIIDRNTTIPTKKSQVFST 423

HSP 2 Score: 55.4546 bits (132), Expect = 4.808e-26
Identity = 35/88 (39.77%), Postives = 51/88 (57.95%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL---YEGIDFYT-TITRARFEELNMDLFRKCMEPVEK 254
            R+VN+   EF+++   D+  +  AL+RL+ A E+AK  LSST QT I +  +     G    T  +TRA+FE L  DL +K +EP  K
Sbjct:  230 RLVNYLADEFQKEQGIDLRKDKLALQRLKEAAEKAKIELSSTTQTEINLPFITADQSGPKHLTMKLTRAKFEALVDDLVQKTIEPCRK 317
[Close]
BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_RHOPA (Chaperone protein dnaK OS=Rhodopseudomonas palustris GN=dnaK PE=2 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 4.808e-26
Identity = 48/109 (44.04%), Postives = 62/109 (56.88%), Query Frame = 1 
Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +    + +VVLVGG TR+PKVQ++++  F GKE  K +NPDE           +L G+    V+D             ET GGV T +I RNTTIPTKK QVFST
Sbjct:  320 KDAGLTAGEISEVVLVGGMTRMPKVQEVVKQLF-GKEPHKGVNPDEVVAIGAAIQAGVLQGD----VKDVLLLDVTPLSLGIETLGGVFTRIIDRNTTIPTKKSQVFST 423

HSP 2 Score: 55.4546 bits (132), Expect = 4.808e-26
Identity = 35/88 (39.77%), Postives = 51/88 (57.95%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL---YEGIDFYT-TITRARFEELNMDLFRKCMEPVEK 254
            R+VN+   EF+++   D+  +  AL+RL+ A E+AK  LSST QT I +  +     G    T  +TRA+FE L  DL +K +EP  K
Sbjct:  230 RLVNYLADEFQKEQGIDLRKDKLALQRLKEAAEKAKIELSSTTQTEINLPFITADQSGPKHLTMKLTRAKFEALVDDLVQKTIEPCRK 317
[Close]
BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_CLOBM (Chaperone protein dnaK OS=Clostridium botulinum (strain Loch Maree / Type A3) GN=dnaK PE=2 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 4.809e-26
Identity = 47/102 (46.08%), Postives = 59/102 (57.84%), Query Frame = 1 
Query:  280 SSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            S +  +++VGGSTRIP VQ  ++DF  GKEL K +NPDE           +L+GE    V+D             ET GGV T LI +NTTIPT+K QVFST
Sbjct:  304 SDIDKIIMVGGSTRIPAVQDAVKDF-TGKELSKGVNPDEVVAMGAAIQAGVLTGE----VKDVLLLDVTPLTLGIETFGGVSTTLIEKNTTIPTRKSQVFST 400

HSP 2 Score: 55.0694 bits (131), Expect = 4.809e-26
Identity = 30/90 (33.33%), Postives = 53/90 (58.89%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLY------EGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            +++++  + FK +N  D+  +  A++RL+ A E+AK  LSS  QT I +  +       + ID    +TRA+F EL  DL ++ +EP++K
Sbjct:  207 KLIDYIAETFKAENGIDLRNDKMAIQRLKEAAEKAKIELSSATQTNINLPFITADATGPKHIDM--NLTRAKFNELTHDLVQRTLEPIKK 294
[Close]
BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_CLOBL (Chaperone protein dnaK OS=Clostridium botulinum (strain Langeland / NCTC 10281 / Type F) GN=dnaK PE=2 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 4.809e-26
Identity = 47/102 (46.08%), Postives = 59/102 (57.84%), Query Frame = 1 
Query:  280 SSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            S +  +++VGGSTRIP VQ  ++DF  GKEL K +NPDE           +L+GE    V+D             ET GGV T LI +NTTIPT+K QVFST
Sbjct:  304 SDIDKIIMVGGSTRIPAVQDAVKDF-TGKELSKGVNPDEVVAMGAAIQAGVLTGE----VKDVLLLDVTPLTLGIETFGGVSTTLIEKNTTIPTRKSQVFST 400

HSP 2 Score: 55.0694 bits (131), Expect = 4.809e-26
Identity = 30/90 (33.33%), Postives = 53/90 (58.89%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLY------EGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            +++++  + FK +N  D+  +  A++RL+ A E+AK  LSS  QT I +  +       + ID    +TRA+F EL  DL ++ +EP++K
Sbjct:  207 KLIDYIAETFKAENGIDLRNDKMAIQRLKEAAEKAKIELSSATQTNINLPFITADATGPKHIDM--NLTRAKFNELTHDLVQRTLEPIKK 294
[Close]
BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_CLOBK (Chaperone protein dnaK OS=Clostridium botulinum (strain Okra / Type B1) GN=dnaK PE=2 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 4.809e-26
Identity = 47/102 (46.08%), Postives = 59/102 (57.84%), Query Frame = 1 
Query:  280 SSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            S +  +++VGGSTRIP VQ  ++DF  GKEL K +NPDE           +L+GE    V+D             ET GGV T LI +NTTIPT+K QVFST
Sbjct:  304 SDIDKIIMVGGSTRIPAVQDAVKDF-TGKELSKGVNPDEVVAMGAAIQAGVLTGE----VKDVLLLDVTPLTLGIETFGGVSTTLIEKNTTIPTRKSQVFST 400

HSP 2 Score: 55.0694 bits (131), Expect = 4.809e-26
Identity = 30/90 (33.33%), Postives = 53/90 (58.89%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLY------EGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            +++++  + FK +N  D+  +  A++RL+ A E+AK  LSS  QT I +  +       + ID    +TRA+F EL  DL ++ +EP++K
Sbjct:  207 KLIDYIAETFKAENGIDLRNDKMAIQRLKEAAEKAKIELSSATQTNINLPFITADATGPKHIDM--NLTRAKFNELTHDLVQRTLEPIKK 294
[Close]
BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_CLOBJ (Chaperone protein dnaK OS=Clostridium botulinum (strain Kyoto / Type A2) GN=dnaK PE=2 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 4.809e-26
Identity = 47/102 (46.08%), Postives = 59/102 (57.84%), Query Frame = 1 
Query:  280 SSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            S +  +++VGGSTRIP VQ  ++DF  GKEL K +NPDE           +L+GE    V+D             ET GGV T LI +NTTIPT+K QVFST
Sbjct:  304 SDIDKIIMVGGSTRIPAVQDAVKDF-TGKELSKGVNPDEVVAMGAAIQAGVLTGE----VKDVLLLDVTPLTLGIETFGGVSTTLIEKNTTIPTRKSQVFST 400

HSP 2 Score: 55.0694 bits (131), Expect = 4.809e-26
Identity = 30/90 (33.33%), Postives = 53/90 (58.89%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLY------EGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            +++++  + FK +N  D+  +  A++RL+ A E+AK  LSS  QT I +  +       + ID    +TRA+F EL  DL ++ +EP++K
Sbjct:  207 KLIDYIAETFKAENGIDLRNDKMAIQRLKEAAEKAKIELSSATQTNINLPFITADATGPKHIDM--NLTRAKFNELTHDLVQRTLEPIKK 294
[Close]
BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_CLOBH (Chaperone protein dnaK OS=Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A) GN=dnaK PE=2 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 4.809e-26
Identity = 47/102 (46.08%), Postives = 59/102 (57.84%), Query Frame = 1 
Query:  280 SSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            S +  +++VGGSTRIP VQ  ++DF  GKEL K +NPDE           +L+GE    V+D             ET GGV T LI +NTTIPT+K QVFST
Sbjct:  304 SDIDKIIMVGGSTRIPAVQDAVKDF-TGKELSKGVNPDEVVAMGAAIQAGVLTGE----VKDVLLLDVTPLTLGIETFGGVSTTLIEKNTTIPTRKSQVFST 400

HSP 2 Score: 55.0694 bits (131), Expect = 4.809e-26
Identity = 30/90 (33.33%), Postives = 53/90 (58.89%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLY------EGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            +++++  + FK +N  D+  +  A++RL+ A E+AK  LSS  QT I +  +       + ID    +TRA+F EL  DL ++ +EP++K
Sbjct:  207 KLIDYIAETFKAENGIDLRNDKMAIQRLKEAAEKAKIELSSATQTNINLPFITADATGPKHIDM--NLTRAKFNELTHDLVQRTLEPIKK 294
[Close]
BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_CLOB6 (Chaperone protein dnaK OS=Clostridium botulinum (strain 657 / Type Ba4) GN=dnaK PE=2 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 4.809e-26
Identity = 47/102 (46.08%), Postives = 59/102 (57.84%), Query Frame = 1 
Query:  280 SSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            S +  +++VGGSTRIP VQ  ++DF  GKEL K +NPDE           +L+GE    V+D             ET GGV T LI +NTTIPT+K QVFST
Sbjct:  304 SDIDKIIMVGGSTRIPAVQDAVKDF-TGKELSKGVNPDEVVAMGAAIQAGVLTGE----VKDVLLLDVTPLTLGIETFGGVSTTLIEKNTTIPTRKSQVFST 400

HSP 2 Score: 55.0694 bits (131), Expect = 4.809e-26
Identity = 30/90 (33.33%), Postives = 53/90 (58.89%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLY------EGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            +++++  + FK +N  D+  +  A++RL+ A E+AK  LSS  QT I +  +       + ID    +TRA+F EL  DL ++ +EP++K
Sbjct:  207 KLIDYIAETFKAENGIDLRNDKMAIQRLKEAAEKAKIELSSATQTNINLPFITADATGPKHIDM--NLTRAKFNELTHDLVQRTLEPIKK 294
[Close]
BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_CLOB1 (Chaperone protein dnaK OS=Clostridium botulinum (strain ATCC 19397 / Type A) GN=dnaK PE=2 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 4.809e-26
Identity = 47/102 (46.08%), Postives = 59/102 (57.84%), Query Frame = 1 
Query:  280 SSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            S +  +++VGGSTRIP VQ  ++DF  GKEL K +NPDE           +L+GE    V+D             ET GGV T LI +NTTIPT+K QVFST
Sbjct:  304 SDIDKIIMVGGSTRIPAVQDAVKDF-TGKELSKGVNPDEVVAMGAAIQAGVLTGE----VKDVLLLDVTPLTLGIETFGGVSTTLIEKNTTIPTRKSQVFST 400

HSP 2 Score: 55.0694 bits (131), Expect = 4.809e-26
Identity = 30/90 (33.33%), Postives = 53/90 (58.89%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLY------EGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            +++++  + FK +N  D+  +  A++RL+ A E+AK  LSS  QT I +  +       + ID    +TRA+F EL  DL ++ +EP++K
Sbjct:  207 KLIDYIAETFKAENGIDLRNDKMAIQRLKEAAEKAKIELSSATQTNINLPFITADATGPKHIDM--NLTRAKFNELTHDLVQRTLEPIKK 294
The following BLAST results are available for this feature:
BLAST of CB417297 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
DNAK_RHOS74.808e-2644.04Chaperone protein dnaK OS=Rhodopseudomonas sp. (st... [more]
DNAK_RHOPT4.808e-2644.04Chaperone protein dnaK OS=Rhodopseudomonas palustr... [more]
DNAK_RHOPA4.808e-2644.04Chaperone protein dnaK OS=Rhodopseudomonas palustr... [more]
DNAK_CLOBM4.809e-2646.08Chaperone protein dnaK OS=Clostridium botulinum (s... [more]
DNAK_CLOBL4.809e-2646.08Chaperone protein dnaK OS=Clostridium botulinum (s... [more]
DNAK_CLOBK4.809e-2646.08Chaperone protein dnaK OS=Clostridium botulinum (s... [more]
DNAK_CLOBJ4.809e-2646.08Chaperone protein dnaK OS=Clostridium botulinum (s... [more]
DNAK_CLOBH4.809e-2646.08Chaperone protein dnaK OS=Clostridium botulinum (s... [more]
DNAK_CLOB64.809e-2646.08Chaperone protein dnaK OS=Clostridium botulinum (s... [more]
DNAK_CLOB14.809e-2646.08Chaperone protein dnaK OS=Clostridium botulinum (s... [more]

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Properties
Property NameValue
Genbank descriptionEST0370 Mature peel (flavedo) cDNA subtraction library Citrus sinensis cDNA clone 6P015 similar to 70 kDa heat shock protein (acc# AAL24367), mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CB417297 ID=CB417297; Name=CB417297; organism=Citrus sinensis; type=EST; length=590bp
ACAGAATGGTGAACCACTTTGTTCAAGAGTTCAAGAGGAAAAACAAGAAG
GATATTAGTGGAAACCCCAGAGCGTTGAGGAGGTTGAGAACTGCCTGTGA
AAGGGCTAAGAGGACCCTGTCATCAACCGCACAGACTACAATTGAGATTG
ATTCTTTATATGAGGGTATTGATTTCTACACAACCATTACAAGGGCTAGA
TTCGAAGAGTTGAACATGGATTTGTTCAGGAAGTGTATGGAGCCAGTGGA
GAAATGCGAGAGATGCTAAGATGGACAAGAGCAGTGTCCATGATGTGGTT
CTTGTTGGTGGGTCTACTAGGATCCCAAAAGTCCAGCAACTGTTGCAGGA
CTTCTTCAATGGAAAAGAGCTATGCAAGAGCATCAACCCTGATGAAGCGG
TTGCCTACGGTGCTGCTGTGCAGGCTGCAATCTTGAGTGGTGAGGGTAAT
GAGAAGGTTCAGGACCTCTTATTGCTTGACGTCACCCCTCTATCGCTGGG
TTTGGAGACTGCTGGTGGAGTCATGACTGTGTTGATTCCAAGGAACACCA
CTATTCCAACCAAGAAGGAGCAGGTGTTCTCCACATACTC
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