CB417297 | Citrus Genome Database

Overview


Name	CB417297
Unique Name	CB417297
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	590

Libraries


Library Name	Type
Mature peel (flavedo) cDNA subtraction library	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_STRCO (Chaperone protein dnaK OS=Streptomyces coelicolor GN=dnaK PE=2 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 4.810e-26
Identity = 45/109 (41.28%), Postives = 62/109 (56.88%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +  S +  VVLVGGSTR+P V +L+++   GK+  K +NPDE           +L GE    V+D             ET GG+MT LI RNTTIPTK+ ++F+T
Sbjct:  295 KDAGIQLSEIDHVVLVGGSTRMPAVAELVKELTGGKDANKGVNPDEVVAIGAALQAGVLKGE----VKDVLLLDVTPLSLGIETKGGIMTKLIERNTTIPTKRSEIFTT 399

HSP 2 Score: 55.0694 bits (131), Expect = 4.810e-26
Identity = 32/85 (37.65%), Postives = 53/85 (62.35%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIE---IDSLYEG-IDFYTTITRARFEELNMDLFRKCMEP 245
            R+V++ V++F+  +  D++ +  AL+RLR A E+AK  LSS+ +TTI    I +  EG +     +TRA+F++L  DL  +C  P
Sbjct:  205 RVVDYLVKQFQSGHGVDLAKDKMALQRLREAAEKAKIELSSSTETTINLPYITASAEGPLHLDEKLTRAQFQQLTSDLLERCKTP 289

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_PHATR (Chaperone protein dnaK OS=Phaeodactylum tricornutum GN=dnaK PE=3 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 4.810e-26
Identity = 48/112 (42.86%), Postives = 68/112 (60.71%), Query Frame = 1

Query:  250 RNARDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            ++  DA++DK+ ++++VLVGGSTRIP VQ L++    GK+  +S+NPDE           IL+GE    ++D             ET GGVMT +I RNTTIP KK ++FST
Sbjct:  317 KSLNDARLDKADINEIVLVGGSTRIPAVQNLVES-LTGKKPNQSVNPDEVVAIGAAIQAGILAGE----IKDILLLDVTPLSLGVETLGGVMTKIIARNTTIPVKKSEMFST 423

HSP 2 Score: 50.8322 bits (120), Expect = 4.810e-26
Identity = 31/87 (35.63%), Postives = 45/87 (51.72%), Query Frame = 3

Query:    6 MVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEG----IDFYTTITRARFEELNMDLFRKCMEPVEK 254
            +VN  + +FK KN  D+  + +AL+RL  A E+AK  LS+  +TTI +  +             +TR  FE L   L  +C  PVEK
Sbjct:  231 LVNWLINDFKEKNNIDLGNDVQALQRLTEAAEKAKVELSTVEKTTIHLPFITADKTGPKHIEQELTRDVFENLCQALINRCRIPVEK 317

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DHE2_ACHKL (NAD-specific glutamate dehydrogenase OS=Achlya klebsiana PE=3 SV=1)

HSP 1 Score: 95.9005 bits (237), Expect = 6.209e-26
Identity = 54/111 (48.65%), Postives = 71/111 (63.96%), Query Frame = -3

Query:  259 VCGEHLLLLGWNSGVPWNQHSHDSTSSLQTQR*RGDVKQ*EVLNLLI-TLTTQDCSLHSSTVGNRFIRVDALA*LFSIEEVLQQLLDFWDPSRPTNKNHIMDTALVHLSIS 588
            V GE L LLGW+S V  ++ SHD+TSS QT+R R D+K+ +VL L    +T Q+ SL   T  N FIRVD LA   ++EEV +QLLD WD    T+K+  MD AL  L ++
Sbjct:  610 VGGESLRLLGWHSSVTLDEGSHDTTSSFQTKRERSDIKKQQVLELFRRVVTAQNGSLDCGTESNSFIRVDRLAWFLAVEEVRKQLLDLWDTGGTTDKDDFMDLALGELRVT 720

HSP 2 Score: 42.3578 bits (98), Expect = 6.209e-26
Identity = 27/76 (35.53%), Postives = 41/76 (53.95%), Query Frame = -1

Query:   12 HTLPEQIHVQLFESSPCNGCVEINTLI*RINLNCSLCG**QGPLSPFTGSSQPPQRSGVSTNILLVFPLELLNKVV 239
            H+L E +   +FE+   +G VEINT+  R++ N SL    +  L  FT  ++  + + V  +IL V  LE   KVV
Sbjct:  728 HSLAEVVTAHVFETGTGDGGVEINTIEERVDFNVSLGRRRKSTLGTFTSGTKTAKGTLVLGHILAVLALEFSGKVV 803

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_METFK (Chaperone protein dnaK OS=Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875) GN=dnaK PE=2 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 6.257e-26
Identity = 50/109 (45.87%), Postives = 64/109 (58.72%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +  S + DV+LVGG TR+PKVQ+ +++FF GKE  K +NPDE           +L G+    V+D             ET GGVMT LI +NTTIPTK  QVFST
Sbjct:  325 KDAGVKVSDIQDVILVGGQTRMPKVQEKVKEFF-GKEPRKDVNPDEAVAVGAAIQGGVLQGD----VKDVLLLDVTPLSLGIETLGGVMTKLIQKNTTIPTKASQVFST 428

HSP 2 Score: 50.8322 bits (120), Expect = 6.257e-26
Identity = 29/87 (33.33%), Postives = 47/87 (54.02%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGID----FYTTITRARFEELNMDLFRKCMEPVE 251
            R+++    EFK++N  D+  +  A +RL+ A E+AK  LSS+ QT + +  +             ITRA+FE L  DL  + ++P E
Sbjct:  235 RLIDFLADEFKKENGIDLRNDLLAKQRLKEAAEKAKIELSSSTQTEVNLPYITADASGPKHLVVKITRAKFESLVEDLIERSIKPCE 321

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_SHESH (Chaperone protein dnaK OS=Shewanella sediminis (strain HAW-EB3) GN=dnaK PE=2 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 6.257e-26
Identity = 47/108 (43.52%), Postives = 62/108 (57.41%), Query Frame = 1

Query:  262 DAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            DA +  S +++V+LVGG TR+PKV+  + +FF GKEL + +NPDE           +LSG+    V+D             ET G VMT LI +NTTIPTK  Q FST
Sbjct:  325 DADLSVSDINEVILVGGQTRMPKVRTEVSEFF-GKELRQDVNPDEAVAIGAAVQAGVLSGD----VKDVLLLDVTPLSLGIETMGSVMTKLIEKNTTIPTKASQTFST 427

HSP 2 Score: 56.225 bits (134), Expect = 6.257e-26
Identity = 29/87 (33.33%), Postives = 49/87 (56.32%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGI----DFYTTITRARFEELNMDLFRKCMEPVE 251
            R++N+   EFK++   D+  +P A++RL+ A E+AK  LSS +QT + +  +             ITRA+ E L  DL  + +EP++
Sbjct:  234 RLINYLADEFKKEQSLDLRNDPLAMQRLKEAAEKAKIELSSASQTEVNLPYITADATGPKHLVVKITRAKLESLVEDLITRTLEPLK 320

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_XYLFT (Chaperone protein dnaK OS=Xylella fastidiosa (strain Temecula1 / ATCC 700964) GN=dnaK PE=2 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 6.257e-26
Identity = 50/108 (46.30%), Postives = 62/108 (57.41%), Query Frame = 1

Query:  262 DAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            DA +  S V +V+LVGG TR+PKVQQ + DFF GKE  K +NPDE           +L+G+    V+D             ET GGV T +I +NTTIPTK  QVFST
Sbjct:  327 DAGLRTSDVQEVILVGGQTRMPKVQQAVADFF-GKEPRKDVNPDEAVALGAAIQGGVLAGD----VKDVLLLDVTPLSLGIETMGGVFTKIIEKNTTIPTKASQVFST 429

HSP 2 Score: 52.373 bits (124), Expect = 6.257e-26
Identity = 31/85 (36.47%), Postives = 47/85 (55.29%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGID----FYTTITRARFEELNMDLFRKCMEP 245
            R++++ V EF +    D+  +P AL+RL+ A ERAK  LSS+ QT + +  +             +TRA+ E L  DL RK +EP
Sbjct:  236 RVIDYLVDEFNKDQGIDLRKDPLALQRLKDAAERAKIELSSSQQTEVNLPYITADASGPKHLNIKLTRAKLEALVDDLVRKSIEP 320

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_XYLFM (Chaperone protein dnaK OS=Xylella fastidiosa (strain M12) GN=dnaK PE=2 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 6.257e-26
Identity = 50/108 (46.30%), Postives = 62/108 (57.41%), Query Frame = 1

Query:  262 DAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            DA +  S V +V+LVGG TR+PKVQQ + DFF GKE  K +NPDE           +L+G+    V+D             ET GGV T +I +NTTIPTK  QVFST
Sbjct:  327 DAGLRTSDVQEVILVGGQTRMPKVQQAVADFF-GKEPRKDVNPDEAVALGAAIQGGVLAGD----VKDVLLLDVTPLSLGIETMGGVFTKIIEKNTTIPTKASQVFST 429

HSP 2 Score: 52.373 bits (124), Expect = 6.257e-26
Identity = 31/85 (36.47%), Postives = 47/85 (55.29%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGID----FYTTITRARFEELNMDLFRKCMEP 245
            R++++ V EF +    D+  +P AL+RL+ A ERAK  LSS+ QT + +  +             +TRA+ E L  DL RK +EP
Sbjct:  236 RVIDYLVDEFNKDQGIDLRKDPLALQRLKDAAERAKIELSSSQQTEVNLPYITADASGPKHLNIKLTRAKLEALVDDLVRKSIEP 320

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_XYLFA (Chaperone protein dnaK OS=Xylella fastidiosa GN=dnaK PE=2 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 6.257e-26
Identity = 50/108 (46.30%), Postives = 62/108 (57.41%), Query Frame = 1

Query:  262 DAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            DA +  S V +V+LVGG TR+PKVQQ + DFF GKE  K +NPDE           +L+G+    V+D             ET GGV T +I +NTTIPTK  QVFST
Sbjct:  327 DAGLRTSDVQEVILVGGQTRMPKVQQAVADFF-GKEPRKDVNPDEAVALGAAIQGGVLAGD----VKDVLLLDVTPLSLGIETMGGVFTKIIEKNTTIPTKASQVFST 429

HSP 2 Score: 52.373 bits (124), Expect = 6.257e-26
Identity = 31/85 (36.47%), Postives = 47/85 (55.29%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGID----FYTTITRARFEELNMDLFRKCMEP 245
            R++++ V EF +    D+  +P AL+RL+ A ERAK  LSS+ QT + +  +             +TRA+ E L  DL RK +EP
Sbjct:  236 RVIDYLVDEFNKDQGIDLRKDPLALQRLKDAAERAKIELSSSQQTEVNLPYITADASGPKHLNIKLTRAKLEALVDDLVRKSIEP 320

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_XYLF2 (Chaperone protein dnaK OS=Xylella fastidiosa (strain M23) GN=dnaK PE=2 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 6.257e-26
Identity = 50/108 (46.30%), Postives = 62/108 (57.41%), Query Frame = 1

Query:  262 DAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            DA +  S V +V+LVGG TR+PKVQQ + DFF GKE  K +NPDE           +L+G+    V+D             ET GGV T +I +NTTIPTK  QVFST
Sbjct:  327 DAGLRTSDVQEVILVGGQTRMPKVQQAVADFF-GKEPRKDVNPDEAVALGAAIQGGVLAGD----VKDVLLLDVTPLSLGIETMGGVFTKIIEKNTTIPTKASQVFST 429

HSP 2 Score: 52.373 bits (124), Expect = 6.257e-26
Identity = 31/85 (36.47%), Postives = 47/85 (55.29%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGID----FYTTITRARFEELNMDLFRKCMEP 245
            R++++ V EF +    D+  +P AL+RL+ A ERAK  LSS+ QT + +  +             +TRA+ E L  DL RK +EP
Sbjct:  236 RVIDYLVDEFNKDQGIDLRKDPLALQRLKDAAERAKIELSSSQQTEVNLPYITADASGPKHLNIKLTRAKLEALVDDLVRKSIEP 320

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_ERWT9 (Chaperone protein dnaK OS=Erwinia tasmaniensis (strain DSM 17950 / Et1/99) GN=dnaK PE=2 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 6.257e-26
Identity = 50/109 (45.87%), Postives = 63/109 (57.80%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +  S ++DV+LVGG TR+P VQ  + +FF GKE  K +NPDE           +L+GE    V+D             ET GGVMT LI +NTTIPTK  QVFST
Sbjct:  325 QDAGLSVSDINDVILVGGQTRMPMVQAKVAEFF-GKEPRKDVNPDEAVAVGAAVQGGVLAGE----VKDVLLLDVTPLSLGIETMGGVMTSLITKNTTIPTKHSQVFST 428

HSP 2 Score: 52.7582 bits (125), Expect = 6.257e-26
Identity = 29/87 (33.33%), Postives = 48/87 (55.17%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGI----DFYTTITRARFEELNMDLFRKCMEPVE 251
            RM+N+ V EFK+    D+  +P A++RL+ A E+AK  LSS  QT + +  +             +TRA+ E L  DL  + ++P++
Sbjct:  235 RMINYLVAEFKKDQGIDLHNDPLAMQRLKEAAEKAKIELSSAQQTDVNLPYITADATGPKHLNIKVTRAKLESLVEDLVTRSIDPLK 321

The following BLAST results are available for this feature:

BLAST of CB417297 vs. ExPASy Swiss-Prot

BLAST of CB417297 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
DNAK_STRCO	4.810e-26	41.28	Chaperone protein dnaK OS=Streptomyces coelicolor ...	[more]
DNAK_PHATR	4.810e-26	42.86	Chaperone protein dnaK OS=Phaeodactylum tricornutu...	[more]
DHE2_ACHKL	6.209e-26	48.65	NAD-specific glutamate dehydrogenase OS=Achlya kle...	[more]
DNAK_METFK	6.257e-26	45.87	Chaperone protein dnaK OS=Methylobacillus flagella...	[more]
DNAK_SHESH	6.257e-26	43.52	Chaperone protein dnaK OS=Shewanella sediminis (st...	[more]
DNAK_XYLFT	6.257e-26	46.30	Chaperone protein dnaK OS=Xylella fastidiosa (stra...	[more]
DNAK_XYLFM	6.257e-26	46.30	Chaperone protein dnaK OS=Xylella fastidiosa (stra...	[more]
DNAK_XYLFA	6.257e-26	46.30	Chaperone protein dnaK OS=Xylella fastidiosa GN=dn...	[more]
DNAK_XYLF2	6.257e-26	46.30	Chaperone protein dnaK OS=Xylella fastidiosa (stra...	[more]
DNAK_ERWT9	6.257e-26	45.87	Chaperone protein dnaK OS=Erwinia tasmaniensis (st...	[more]

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Properties


Property Name	Value
Genbank description	EST0370 Mature peel (flavedo) cDNA subtraction library Citrus sinensis cDNA clone 6P015 similar to 70 kDa heat shock protein (acc# AAL24367), mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CB417297 ID=CB417297; Name=CB417297; organism=Citrus sinensis; type=EST; length=590bp
ACAGAATGGTGAACCACTTTGTTCAAGAGTTCAAGAGGAAAAACAAGAAG
GATATTAGTGGAAACCCCAGAGCGTTGAGGAGGTTGAGAACTGCCTGTGA
AAGGGCTAAGAGGACCCTGTCATCAACCGCACAGACTACAATTGAGATTG
ATTCTTTATATGAGGGTATTGATTTCTACACAACCATTACAAGGGCTAGA
TTCGAAGAGTTGAACATGGATTTGTTCAGGAAGTGTATGGAGCCAGTGGA
GAAATGCGAGAGATGCTAAGATGGACAAGAGCAGTGTCCATGATGTGGTT
CTTGTTGGTGGGTCTACTAGGATCCCAAAAGTCCAGCAACTGTTGCAGGA
CTTCTTCAATGGAAAAGAGCTATGCAAGAGCATCAACCCTGATGAAGCGG
TTGCCTACGGTGCTGCTGTGCAGGCTGCAATCTTGAGTGGTGAGGGTAAT
GAGAAGGTTCAGGACCTCTTATTGCTTGACGTCACCCCTCTATCGCTGGG
TTTGGAGACTGCTGGTGGAGTCATGACTGTGTTGATTCCAAGGAACACCA
CTATTCCAACCAAGAAGGAGCAGGTGTTCTCCACATACTC