CB417297 | Citrus Genome Database

Overview


Name	CB417297
Unique Name	CB417297
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	590

Libraries


Library Name	Type
Mature peel (flavedo) cDNA subtraction library	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_PELLD (Chaperone protein dnaK OS=Pelodictyon luteolum (strain DSM 273) GN=dnaK PE=2 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 1.060e-25
Identity = 50/112 (44.64%), Postives = 68/112 (60.71%), Query Frame = 1

Query:  250 RNARDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            R  +++K++   + ++VLVGGSTRIPKVQ L+++FF GKE  +S+NPDE           +L G+    V D             ET GGVMT LI  NTTIPT+K++VFST
Sbjct:  315 RAVKNSKVEMKDIDEIVLVGGSTRIPKVQALVKEFF-GKEPNRSVNPDEVVAIGAAIQGGVLKGD----VTDVLLLDVTPLSLGIETLGGVMTKLIDANTTIPTRKQEVFST 421

HSP 2 Score: 48.1358 bits (113), Expect = 1.060e-25
Identity = 31/88 (35.23%), Postives = 48/88 (54.55%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIE---IDSLYEG-IDFYTTITRARFEELNMDLFRKCMEPVEK 254
            ++++    EFK++   D+  +  AL+RL+ A E+AK  LSS   T I    I +  EG       +TRA+FE +  DLF K +EP  +
Sbjct:  228 KIIDFLADEFKKQEGVDLRNDAIALQRLKEAGEKAKVELSSRTDTEINLPFITATPEGPKHLVINLTRAKFEGMCSDLFDKILEPCRR 315

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_BDEBA (Chaperone protein dnaK OS=Bdellovibrio bacteriovorus GN=dnaK PE=2 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 1.060e-25
Identity = 50/108 (46.30%), Postives = 65/108 (60.19%), Query Frame = 1

Query:  262 DAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            D+ +  S + +VVLVGGSTRIP +Q+ ++DFF GKE  +S+NPDE           +L+G+    V+D             ET GGVMT LI RNT IP+KK QVFST
Sbjct:  318 DSGLKASEIDEVVLVGGSTRIPAIQKAVKDFF-GKEPNRSVNPDEVVAIGAAVQGGVLAGD----VKDVLLLDVTPLSLGIETLGGVMTTLIERNTAIPSKKSQVFST 420

HSP 2 Score: 49.6766 bits (117), Expect = 1.060e-25
Identity = 27/82 (32.93%), Postives = 45/82 (54.88%), Query Frame = 3

Query:   21 VQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGID----FYTTITRARFEELNMDLFRKCMEPVEK 254
            + EFK+    D+  +  AL+RL+ A E+AK  LSS  +T I +  +           T ++RA+F+++  DL ++ MEP  K
Sbjct:  233 ITEFKKDQAIDLKNDKMALQRLKEAAEKAKIELSSAQETEINLPFITADQSGPKHLQTRLSRAKFDQMTEDLVKRSMEPCRK 314

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_METPB (Chaperone protein dnaK OS=Methylobacterium populi (strain ATCC BAA-705 / NCIMB 13946 / BJ001) GN=dnaK PE=2 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 1.060e-25
Identity = 51/109 (46.79%), Postives = 64/109 (58.72%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +  S + +VVLVGG TR+PKVQ++++ FF GKE  K +NPDE           +L G+    V+D             ET GGV T LI RNTTIPTKK QVFST
Sbjct:  320 KDAGVSASEIDEVVLVGGQTRMPKVQEVVKAFF-GKEPHKGVNPDEVVAIGAAVQAGVLQGD----VKDVLLLDVTPLSLGIETLGGVFTRLIDRNTTIPTKKSQVFST 423

HSP 2 Score: 50.0618 bits (118), Expect = 1.060e-25
Identity = 30/88 (34.09%), Postives = 48/88 (54.55%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGID----FYTTITRARFEELNMDLFRKCMEPVEK 254
            R+V +   EFK++   D++ +  AL+RL+ A E+AK  LSS  QT I +  +             ++RA+FE L  DL ++ +EP  K
Sbjct:  230 RVVEYLTAEFKKEQGIDLTKDKLALQRLKEAAEKAKIELSSATQTEINLPYITADASGPKHLALKLSRAKFESLVDDLVQRTIEPCRK 317

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_METEP (Chaperone protein dnaK OS=Methylobacterium extorquens (strain PA1) GN=dnaK PE=2 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 1.060e-25
Identity = 51/109 (46.79%), Postives = 64/109 (58.72%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +  S + +VVLVGG TR+PKVQ++++ FF GKE  K +NPDE           +L G+    V+D             ET GGV T LI RNTTIPTKK QVFST
Sbjct:  320 KDAGVSASEIDEVVLVGGQTRMPKVQEVVKAFF-GKEPHKGVNPDEVVAIGAAVQAGVLQGD----VKDVLLLDVTPLSLGIETLGGVFTRLIDRNTTIPTKKSQVFST 423

HSP 2 Score: 50.0618 bits (118), Expect = 1.060e-25
Identity = 30/88 (34.09%), Postives = 48/88 (54.55%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGID----FYTTITRARFEELNMDLFRKCMEPVEK 254
            R+V +   EFK++   D++ +  AL+RL+ A E+AK  LSS  QT I +  +             ++RA+FE L  DL ++ +EP  K
Sbjct:  230 RVVEYLTAEFKKEQGIDLTKDKLALQRLKEAAEKAKIELSSATQTEINLPYITADASGPKHLALKLSRAKFESLVDDLVQRTIEPCRK 317

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_METC4 (Chaperone protein dnaK OS=Methylobacterium chloromethanicum (strain CM4 / NCIMB 13688) GN=dnaK PE=2 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 1.060e-25
Identity = 51/109 (46.79%), Postives = 64/109 (58.72%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +  S + +VVLVGG TR+PKVQ++++ FF GKE  K +NPDE           +L G+    V+D             ET GGV T LI RNTTIPTKK QVFST
Sbjct:  320 KDAGVSASEIDEVVLVGGQTRMPKVQEVVKAFF-GKEPHKGVNPDEVVAIGAAVQAGVLQGD----VKDVLLLDVTPLSLGIETLGGVFTRLIDRNTTIPTKKSQVFST 423

HSP 2 Score: 50.0618 bits (118), Expect = 1.060e-25
Identity = 30/88 (34.09%), Postives = 48/88 (54.55%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGID----FYTTITRARFEELNMDLFRKCMEPVEK 254
            R+V +   EFK++   D++ +  AL+RL+ A E+AK  LSS  QT I +  +             ++RA+FE L  DL ++ +EP  K
Sbjct:  230 RVVEYLTAEFKKEQGIDLTKDKLALQRLKEAAEKAKIELSSATQTEINLPYITADASGPKHLALKLSRAKFESLVDDLVQRTIEPCRK 317

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_BUCBP (Chaperone protein dnaK OS=Buchnera aphidicola subsp. Baizongia pistaciae GN=dnaK PE=2 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 1.060e-25
Identity = 50/109 (45.87%), Postives = 63/109 (57.80%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DAK+  S + DV+LVGG TR+P VQ+ + +FF  KE  K +NPDE           +LSGE    V+D             ET GGVMT LI +NTTIPTK  Q+FST
Sbjct:  325 KDAKLSISDIDDVILVGGQTRMPMVQKKVAEFFK-KEPRKDVNPDEAVAVGAAVQGGVLSGE----VKDVLLLDVTPLSLGIETMGGVMTTLISKNTTIPTKHSQIFST 428

HSP 2 Score: 50.0618 bits (118), Expect = 1.060e-25
Identity = 26/87 (29.89%), Postives = 49/87 (56.32%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGI----DFYTTITRARFEELNMDLFRKCMEPVE 251
            R++N+ V++FK+    D+  +  A++RL+ + E+AK  LSST QT + +  +             +TR++ E L  DL  + +EP++
Sbjct:  235 RLINYLVEDFKKDQGTDLRNDSLAMQRLKESAEKAKIELSSTQQTDVNLPYITADSTGPKHLNIKVTRSKLESLVEDLITRSIEPLK 321

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_BUCAP (Chaperone protein dnaK OS=Buchnera aphidicola subsp. Schizaphis graminum GN=dnaK PE=2 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 1.060e-25
Identity = 49/109 (44.95%), Postives = 62/109 (56.88%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +    ++DV+LVGG TR+P VQ  + DFF GKE  K +NPDE           +LSG+    V+D             ET GG+MT LI +NTTIPTK  QVFST
Sbjct:  325 KDAGLSVGDINDVILVGGQTRMPMVQSKVADFF-GKEPRKDVNPDEAVAVGAAVQGGVLSGD----VKDVLLLDVTPLSLGIETMGGIMTSLINKNTTIPTKHSQVFST 428

HSP 2 Score: 51.9878 bits (123), Expect = 1.060e-25
Identity = 28/87 (32.18%), Postives = 50/87 (57.47%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGID----FYTTITRARFEELNMDLFRKCMEPVE 251
            R++N+ VQEFK++   D+  +  A++RL+ A E+AK  LSS  QT + +  +    +        +TR++ E L  DL  + +EP++
Sbjct:  235 RLINYLVQEFKKEQGIDLRNDSLAMQRLKEAAEKAKIELSSAQQTDVNLPYITADSNGPKHLNIKVTRSKLESLVEDLVMRSIEPLK 321

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_SHEB8 (Chaperone protein dnaK OS=Shewanella baltica (strain OS185) GN=dnaK PE=2 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 1.060e-25
Identity = 48/108 (44.44%), Postives = 64/108 (59.26%), Query Frame = 1

Query:  262 DAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            DA +  + +++V+LVGG TR+PKVQ+ + +FF GKE  K +NPDE           +LSG+    V+D             ET G VMT LI +NTTIPTK +QVFST
Sbjct:  325 DADLSVTDINEVILVGGQTRMPKVQEAVTNFF-GKEPRKDVNPDEAVAVGAAIQAGVLSGD----VKDVLLLDVTPLSLGIETMGSVMTKLIEKNTTIPTKAQQVFST 427

HSP 2 Score: 52.7582 bits (125), Expect = 1.060e-25
Identity = 29/87 (33.33%), Postives = 48/87 (55.17%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGI----DFYTTITRARFEELNMDLFRKCMEPVE 251
            R++N+   EFK++   D+  +P A++RL+ A E+AK  LSST  T + +  +             ITRA+ E L  DL  + +EP++
Sbjct:  234 RLINYLADEFKKEQGLDLRKDPLAMQRLKEAAEKAKIELSSTNHTEVNLPYITADATGPKHLVIKITRAKLESLVEDLILRTLEPLK 320

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_SHEB5 (Chaperone protein dnaK OS=Shewanella baltica (strain OS155 / ATCC BAA-1091) GN=dnaK PE=2 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 1.060e-25
Identity = 48/108 (44.44%), Postives = 64/108 (59.26%), Query Frame = 1

Query:  262 DAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            DA +  + +++V+LVGG TR+PKVQ+ + +FF GKE  K +NPDE           +LSG+    V+D             ET G VMT LI +NTTIPTK +QVFST
Sbjct:  325 DADLSVTDINEVILVGGQTRMPKVQEAVTNFF-GKEPRKDVNPDEAVAVGAAIQAGVLSGD----VKDVLLLDVTPLSLGIETMGSVMTKLIEKNTTIPTKAQQVFST 427

HSP 2 Score: 52.7582 bits (125), Expect = 1.060e-25
Identity = 29/87 (33.33%), Postives = 48/87 (55.17%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGI----DFYTTITRARFEELNMDLFRKCMEPVE 251
            R++N+   EFK++   D+  +P A++RL+ A E+AK  LSST  T + +  +             ITRA+ E L  DL  + +EP++
Sbjct:  234 RLINYLADEFKKEQGLDLRKDPLAMQRLKEAAEKAKIELSSTNHTEVNLPYITADATGPKHLVIKITRAKLESLVEDLILRTLEPLK 320

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_SHEB2 (Chaperone protein dnaK OS=Shewanella baltica (strain OS223) GN=dnaK PE=2 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 1.060e-25
Identity = 48/108 (44.44%), Postives = 64/108 (59.26%), Query Frame = 1

Query:  262 DAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            DA +  + +++V+LVGG TR+PKVQ+ + +FF GKE  K +NPDE           +LSG+    V+D             ET G VMT LI +NTTIPTK +QVFST
Sbjct:  325 DADLSVTDINEVILVGGQTRMPKVQEAVTNFF-GKEPRKDVNPDEAVAVGAAIQAGVLSGD----VKDVLLLDVTPLSLGIETMGSVMTKLIEKNTTIPTKAQQVFST 427

HSP 2 Score: 52.7582 bits (125), Expect = 1.060e-25
Identity = 29/87 (33.33%), Postives = 48/87 (55.17%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGI----DFYTTITRARFEELNMDLFRKCMEPVE 251
            R++N+   EFK++   D+  +P A++RL+ A E+AK  LSST  T + +  +             ITRA+ E L  DL  + +EP++
Sbjct:  234 RLINYLADEFKKEQGLDLRKDPLAMQRLKEAAEKAKIELSSTNHTEVNLPYITADATGPKHLVIKITRAKLESLVEDLILRTLEPLK 320

The following BLAST results are available for this feature:

BLAST of CB417297 vs. ExPASy Swiss-Prot

BLAST of CB417297 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
DNAK_PELLD	1.060e-25	44.64	Chaperone protein dnaK OS=Pelodictyon luteolum (st...	[more]
DNAK_BDEBA	1.060e-25	46.30	Chaperone protein dnaK OS=Bdellovibrio bacteriovor...	[more]
DNAK_METPB	1.060e-25	46.79	Chaperone protein dnaK OS=Methylobacterium populi ...	[more]
DNAK_METEP	1.060e-25	46.79	Chaperone protein dnaK OS=Methylobacterium extorqu...	[more]
DNAK_METC4	1.060e-25	46.79	Chaperone protein dnaK OS=Methylobacterium chlorom...	[more]
DNAK_BUCBP	1.060e-25	45.87	Chaperone protein dnaK OS=Buchnera aphidicola subs...	[more]
DNAK_BUCAP	1.060e-25	44.95	Chaperone protein dnaK OS=Buchnera aphidicola subs...	[more]
DNAK_SHEB8	1.060e-25	44.44	Chaperone protein dnaK OS=Shewanella baltica (stra...	[more]
DNAK_SHEB5	1.060e-25	44.44	Chaperone protein dnaK OS=Shewanella baltica (stra...	[more]
DNAK_SHEB2	1.060e-25	44.44	Chaperone protein dnaK OS=Shewanella baltica (stra...	[more]

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Properties


Property Name	Value
Genbank description	EST0370 Mature peel (flavedo) cDNA subtraction library Citrus sinensis cDNA clone 6P015 similar to 70 kDa heat shock protein (acc# AAL24367), mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CB417297 ID=CB417297; Name=CB417297; organism=Citrus sinensis; type=EST; length=590bp
ACAGAATGGTGAACCACTTTGTTCAAGAGTTCAAGAGGAAAAACAAGAAG
GATATTAGTGGAAACCCCAGAGCGTTGAGGAGGTTGAGAACTGCCTGTGA
AAGGGCTAAGAGGACCCTGTCATCAACCGCACAGACTACAATTGAGATTG
ATTCTTTATATGAGGGTATTGATTTCTACACAACCATTACAAGGGCTAGA
TTCGAAGAGTTGAACATGGATTTGTTCAGGAAGTGTATGGAGCCAGTGGA
GAAATGCGAGAGATGCTAAGATGGACAAGAGCAGTGTCCATGATGTGGTT
CTTGTTGGTGGGTCTACTAGGATCCCAAAAGTCCAGCAACTGTTGCAGGA
CTTCTTCAATGGAAAAGAGCTATGCAAGAGCATCAACCCTGATGAAGCGG
TTGCCTACGGTGCTGCTGTGCAGGCTGCAATCTTGAGTGGTGAGGGTAAT
GAGAAGGTTCAGGACCTCTTATTGCTTGACGTCACCCCTCTATCGCTGGG
TTTGGAGACTGCTGGTGGAGTCATGACTGTGTTGATTCCAAGGAACACCA
CTATTCCAACCAAGAAGGAGCAGGTGTTCTCCACATACTC