CB417297

Overview
NameCB417297
Unique NameCB417297
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length590
Libraries
Library NameType
Mature peel (flavedo) cDNA subtraction librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig13846 contig Csv1_Contig13846:800..1391. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_SODGM (Chaperone protein dnaK OS=Sodalis glossinidius (strain morsitans) GN=dnaK PE=2 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 1.060e-25
Identity = 50/109 (45.87%), Postives = 63/109 (57.80%), Query Frame = 1 
Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +  S + DV+LVGG TR+P VQ+ + DFF GKE  K +NPDE           +L+G+    V+D             ET GGVMT LI +NTTIPTK  QVFST
Sbjct:  325 KDAGLSVSDIKDVILVGGQTRMPLVQKKVTDFF-GKEPRKDVNPDEAVAIGAAVQGGVLAGD----VKDVLLLDVTPLSLGIETMGGVMTPLIAKNTTIPTKHSQVFST 428

HSP 2 Score: 51.6026 bits (122), Expect = 1.060e-25
Identity = 28/87 (32.18%), Postives = 48/87 (55.17%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEG----IDFYTTITRARFEELNMDLFRKCMEPVE 251
            R++N+ V EFK+    D+  +P A++RL+ A E+AK  LSS  QT + +  +             +TRA+ E L  +L  + +EP++
Sbjct:  235 RLINYLVDEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSAQQTDVNLPYITADGSGPKHMNLKVTRAKLESLVEELVNRTLEPLK 321
[Close]
BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_AMOA5 (Chaperone protein dnaK OS=Amoebophilus asiaticus (strain 5a2) GN=dnaK PE=2 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 1.060e-25
Identity = 48/109 (44.04%), Postives = 64/109 (58.72%), Query Frame = 1 
Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +  + + +V+LVGGSTRIPK+Q+ ++ FFN K+  K +NPDE           +L+GE    V+D             ET GGV T LI  NTTIPTKK +VFST
Sbjct:  321 QDAGLTVADIDEVILVGGSTRIPKIQEEVEKFFN-KKPSKGVNPDEVVAVGAAIQGGVLTGE----VKDVLLLDVIPLSFGIETLGGVYTKLIEANTTIPTKKSEVFST 424

HSP 2 Score: 53.5286 bits (127), Expect = 1.060e-25
Identity = 29/88 (32.95%), Postives = 54/88 (61.36%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL--YEGIDFYTT--ITRARFEELNMDLFRKCMEPVEK 254
            ++++   +EFK++   D+  +P AL+RL+ A E+AK  LS ++ T + +  +   +G+  +    ITRA+FE+L   L R+ +EP +K
Sbjct:  231 KLIDWLAEEFKKEESVDLRKDPTALQRLKEAAEKAKIELSGSSTTEVNLPYITAIDGVPKHLVKKITRAQFEQLVDGLVRRTLEPCKK 318
[Close]
BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_BACSK (Chaperone protein dnaK OS=Bacillus clausii (strain KSM-K16) GN=dnaK PE=2 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 1.061e-25
Identity = 49/112 (43.75%), Postives = 60/112 (53.57%), Query Frame = 1 
Query:  250 RNARDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            R   DA +  S +  VVLVGGSTRIP VQ+ ++    GK+  K +NPDE           +L+G+    V+D             ET GGV T LI RNTTIPT K QVFST
Sbjct:  291 RALSDAGLSASDIDKVVLVGGSTRIPAVQEAIKKL-TGKDPHKGVNPDEVVALGAAVQAGVLTGD----VKDVVLLDVTPLSLGIETMGGVFTKLIERNTTIPTSKSQVFST 397

HSP 2 Score: 57.7658 bits (138), Expect = 1.061e-25
Identity = 30/87 (34.48%), Postives = 51/87 (58.62%), Query Frame = 3 
Query:    6 MVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGI----DFYTTITRARFEELNMDLFRKCMEPVEK 254
            +++H V EFK++N  D+S +  A++RL+ A E+AK+ LS   QT I +  +            T+TRA+F+EL+ DL  + + P  +
Sbjct:  205 IMDHLVAEFKKENGIDLSQDKMAMQRLKDAAEKAKKDLSGVTQTQISLPFITADATGPKHLELTLTRAKFDELSADLVERTLGPTRR 291
[Close]
BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_GEOTN (Chaperone protein dnaK OS=Geobacillus thermodenitrificans (strain NG80-2) GN=dnaK PE=2 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 1.061e-25
Identity = 46/109 (42.20%), Postives = 60/109 (55.05%), Query Frame = 1 
Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +  + +  V+LVGGSTRIP VQ+ ++    GKE  K +NPDE           +++GE    V+D             ET GGV T LI RNTTIPT K QVF+T
Sbjct:  294 QDAGLTSADIDKVILVGGSTRIPAVQEAIKREL-GKEPHKGVNPDEVVAIGAAIQGGVIAGE----VKDIVLLDVTPLSLGIETMGGVFTKLIERNTTIPTSKSQVFTT 397

HSP 2 Score: 60.4622 bits (145), Expect = 1.061e-25
Identity = 32/87 (36.78%), Postives = 53/87 (60.92%), Query Frame = 3 
Query:    6 MVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEG----IDFYTTITRARFEELNMDLFRKCMEPVEK 254
            ++++ V +FK++N  D+S +  AL+RL+ A E+AK+ LS   QT I +  +       +   TT+TRA+FEEL+  L  + M PV +
Sbjct:  205 IIDYLVSQFKQENGIDLSKDKMALQRLKDAAEKAKKELSGVTQTQISLPFISANENGPLHLETTLTRAKFEELSAHLVERTMGPVRQ 291
[Close]
BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_RALSO (Chaperone protein dnaK OS=Ralstonia solanacearum GN=dnaK PE=2 SV=2)

HSP 1 Score: 85.1149 bits (209), Expect = 1.379e-25
Identity = 48/109 (44.04%), Postives = 63/109 (57.80%), Query Frame = 1 
Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +  S +HDV+LVGG TR+PKVQ+ +++FF GKE  K +NPDE           +L G+     +D             ET GGVMT +I +NTTIPTK  Q FST
Sbjct:  326 KDAGVKVSDIHDVILVGGMTRMPKVQEKVKEFF-GKEARKDVNPDEAVAVGAAIQGQVLGGDR----KDVLLLDVTPLSLGIETLGGVMTKMIGKNTTIPTKFSQTFST 429

HSP 2 Score: 51.9878 bits (123), Expect = 1.379e-25
Identity = 31/85 (36.47%), Postives = 49/85 (57.65%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGID----FYTTITRARFEELNMDLFRKCMEP 245
            R++++ + EFK+++  D+S +  AL+RL+ A E+AK  LSST QT I +  +             ITRA+ E L  DL  + +EP
Sbjct:  236 RIIDYIIGEFKKESGVDLSKDVLALQRLKDAAEKAKIELSSTQQTEINLPYITADASGPKHLNLKITRAKLEALVEDLIARTIEP 320
[Close]
BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_TOLAT (Chaperone protein dnaK OS=Tolumonas auensis (strain DSM 9187 / TA4) GN=dnaK PE=2 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 1.379e-25
Identity = 49/109 (44.95%), Postives = 62/109 (56.88%), Query Frame = 1 
Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +  S + D++LVGG TR+P VQ+ + DFF GKE  K +NPDE           +L+GE +    D             ET G VMT LI +NTTIPTKK QVFST
Sbjct:  325 KDAGLSVSQLDDIILVGGQTRMPMVQKAVADFF-GKEPRKDVNPDEAVAMGAAIQGAVLAGEKH----DVLLLDVTPLSLGIETMGSVMTTLIEKNTTIPTKKSQVFST 428

HSP 2 Score: 51.9878 bits (123), Expect = 1.379e-25
Identity = 29/87 (33.33%), Postives = 49/87 (56.32%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGI----DFYTTITRARFEELNMDLFRKCMEPVE 251
            R++N+ V EFKR+   D+  +  AL+RL+ + E+AK  LSS  QT + +  +             +TR++ E L  DL +K +EP++
Sbjct:  235 RLINYLVDEFKREQGIDLRKDQLALQRLKDSAEKAKIELSSAQQTEVNLPYITADATGPKHMNIKVTRSKLESLVEDLVKKTIEPLK 321
[Close]
BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_PORGI (Chaperone protein dnaK OS=Porphyromonas gingivalis GN=dnaK PE=2 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 1.379e-25
Identity = 46/109 (42.20%), Postives = 63/109 (57.80%), Query Frame = 1 
Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA M +  + +V+LVGGSTRIP +Q++++  F GK   K +NPDE           +L+GE    V+D             ET GGVMT LI  NTTIPTKK ++F+T
Sbjct:  319 KDAGMSRGDIDEVILVGGSTRIPAIQEIVEKIF-GKAPSKGVNPDEVVAVGAAIQGGVLTGE----VKDVLLLDVTPLSLGIETMGGVMTRLIDANTTIPTKKSEIFTT 422

HSP 2 Score: 53.1434 bits (126), Expect = 1.379e-25
Identity = 31/86 (36.05%), Postives = 50/86 (58.14%), Query Frame = 3 
Query:    6 MVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL--YEGI--DFYTTITRARFEELNMDLFRKCMEPVE 251
            +++   +EFK +   D+  +P A++RL+ A E+AK  LSST+ T I +  +    GI      T+TRA+FE+L   L + C+ P E
Sbjct:  230 IIDWLAEEFKSQEGVDLRQDPMAMQRLKEAAEKAKIELSSTSSTEINLPYIMPVNGIPKHLVMTLTRAKFEQLADRLIQACVAPCE 315
[Close]
BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_PORG3 (Chaperone protein dnaK OS=Porphyromonas gingivalis (strain ATCC 33277 / DSM 20709 / JCM 12257) GN=dnaK PE=2 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 1.379e-25
Identity = 46/109 (42.20%), Postives = 63/109 (57.80%), Query Frame = 1 
Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA M +  + +V+LVGGSTRIP +Q++++  F GK   K +NPDE           +L+GE    V+D             ET GGVMT LI  NTTIPTKK ++F+T
Sbjct:  319 KDAGMSRGDIDEVILVGGSTRIPAIQEIVEKIF-GKAPSKGVNPDEVVAVGAAIQGGVLTGE----VKDVLLLDVTPLSLGIETMGGVMTRLIDANTTIPTKKSEIFTT 422

HSP 2 Score: 53.1434 bits (126), Expect = 1.379e-25
Identity = 31/86 (36.05%), Postives = 50/86 (58.14%), Query Frame = 3 
Query:    6 MVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL--YEGI--DFYTTITRARFEELNMDLFRKCMEPVE 251
            +++   +EFK +   D+  +P A++RL+ A E+AK  LSST+ T I +  +    GI      T+TRA+FE+L   L + C+ P E
Sbjct:  230 IIDWLAEEFKSQEGVDLRQDPMAMQRLKEAAEKAKIELSSTSSTEINLPYIMPVNGIPKHLVMTLTRAKFEQLADRLIQACVAPCE 315
[Close]
BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_OLICO (Chaperone protein dnaK OS=Oligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / OM5) GN=dnaK PE=2 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 1.380e-25
Identity = 48/109 (44.04%), Postives = 63/109 (57.80%), Query Frame = 1 
Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +    + +VVLVGG TR+PK+Q++++ FF GKE  K +NPDE           +L G+    V+D             ET GGV T +I RNTTIPTKK QVFST
Sbjct:  320 KDAGLSAGEISEVVLVGGMTRMPKIQEVVKQFF-GKEPHKGVNPDEVVAIGAAIQAGVLQGD----VKDVLLLDVTPLSLGIETLGGVFTRIIERNTTIPTKKSQVFST 423

HSP 2 Score: 51.6026 bits (122), Expect = 1.380e-25
Identity = 31/88 (35.23%), Postives = 48/88 (54.55%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGID----FYTTITRARFEELNMDLFRKCMEPVEK 254
            R+V +   EF+++   ++  +  AL+RL+ A E+AK  LSST QT I +  +             +TRA+FE L  DL +K +EP  K
Sbjct:  230 RLVGYLADEFQKEQGINLRNDKLALQRLKEAAEKAKIELSSTTQTEINLPFITADASGPKHLTMKLTRAKFEALVDDLVQKTIEPCRK 317
[Close]
BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_METS4 (Chaperone protein dnaK OS=Methylobacterium sp. (strain 4-46) GN=dnaK PE=2 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 1.380e-25
Identity = 50/109 (45.87%), Postives = 63/109 (57.80%), Query Frame = 1 
Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +  S + +VVLVGG TR+PK+Q +++ FF GKE  K +NPDE           +L G+    V+D             ET GGV T LI RNTTIPTKK QVFST
Sbjct:  320 KDAGVAASEIDEVVLVGGMTRMPKIQDVVKTFF-GKEPHKGVNPDEVVAIGAAVQAGVLQGD----VKDVLLLDVTPLSLGIETLGGVFTRLIDRNTTIPTKKSQVFST 423

HSP 2 Score: 51.6026 bits (122), Expect = 1.380e-25
Identity = 31/88 (35.23%), Postives = 48/88 (54.55%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGID----FYTTITRARFEELNMDLFRKCMEPVEK 254
            R+V +   EFKR+   D++ +  AL+RL+ A E+AK  LSS  QT I +  +             ++RA+FE L  DL ++ +EP  K
Sbjct:  230 RIVEYLAAEFKREQGIDLTRDKLALQRLKEAAEKAKIELSSATQTEINLPYITADASGPKHLALKLSRAKFESLVDDLIQRTIEPCRK 317
The following BLAST results are available for this feature:
BLAST of CB417297 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
DNAK_SODGM1.060e-2545.87Chaperone protein dnaK OS=Sodalis glossinidius (st... [more]
DNAK_AMOA51.060e-2544.04Chaperone protein dnaK OS=Amoebophilus asiaticus (... [more]
DNAK_BACSK1.061e-2543.75Chaperone protein dnaK OS=Bacillus clausii (strain... [more]
DNAK_GEOTN1.061e-2542.20Chaperone protein dnaK OS=Geobacillus thermodenitr... [more]
DNAK_RALSO1.379e-2544.04Chaperone protein dnaK OS=Ralstonia solanacearum G... [more]
DNAK_TOLAT1.379e-2544.95Chaperone protein dnaK OS=Tolumonas auensis (strai... [more]
DNAK_PORGI1.379e-2542.20Chaperone protein dnaK OS=Porphyromonas gingivalis... [more]
DNAK_PORG31.379e-2542.20Chaperone protein dnaK OS=Porphyromonas gingivalis... [more]
DNAK_OLICO1.380e-2544.04Chaperone protein dnaK OS=Oligotropha carboxidovor... [more]
DNAK_METS41.380e-2545.87Chaperone protein dnaK OS=Methylobacterium sp. (st... [more]

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Properties
Property NameValue
Genbank descriptionEST0370 Mature peel (flavedo) cDNA subtraction library Citrus sinensis cDNA clone 6P015 similar to 70 kDa heat shock protein (acc# AAL24367), mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CB417297 ID=CB417297; Name=CB417297; organism=Citrus sinensis; type=EST; length=590bp
ACAGAATGGTGAACCACTTTGTTCAAGAGTTCAAGAGGAAAAACAAGAAG
GATATTAGTGGAAACCCCAGAGCGTTGAGGAGGTTGAGAACTGCCTGTGA
AAGGGCTAAGAGGACCCTGTCATCAACCGCACAGACTACAATTGAGATTG
ATTCTTTATATGAGGGTATTGATTTCTACACAACCATTACAAGGGCTAGA
TTCGAAGAGTTGAACATGGATTTGTTCAGGAAGTGTATGGAGCCAGTGGA
GAAATGCGAGAGATGCTAAGATGGACAAGAGCAGTGTCCATGATGTGGTT
CTTGTTGGTGGGTCTACTAGGATCCCAAAAGTCCAGCAACTGTTGCAGGA
CTTCTTCAATGGAAAAGAGCTATGCAAGAGCATCAACCCTGATGAAGCGG
TTGCCTACGGTGCTGCTGTGCAGGCTGCAATCTTGAGTGGTGAGGGTAAT
GAGAAGGTTCAGGACCTCTTATTGCTTGACGTCACCCCTCTATCGCTGGG
TTTGGAGACTGCTGGTGGAGTCATGACTGTGTTGATTCCAAGGAACACCA
CTATTCCAACCAAGAAGGAGCAGGTGTTCTCCACATACTC
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