CB417297 | Citrus Genome Database

Overview


Name	CB417297
Unique Name	CB417297
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	590

Libraries


Library Name	Type
Mature peel (flavedo) cDNA subtraction library	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig13846	contig	Csv1_Contig13846:800..1391.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_EHRSE (Chaperone protein dnaK OS=Ehrlichia sennetsu GN=dnaK PE=2 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 1.380e-25
Identity = 46/109 (42.20%), Postives = 61/109 (55.96%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +D+ + +  +++VVLVGG TR+P V + + +FF GKE  K +NPDE           IL+G  +   QD             ET GGV T LI RNTTIPTK+ Q FST
Sbjct:  319 KDSGLKREEINEVVLVGGMTRMPAVVKKVTEFF-GKEPHKGVNPDEVVAIGAAIQANILAGGSD--AQDIVLLDVTPLSLGIETLGGVFTKLIDRNTTIPTKRSQTFST 424

HSP 2 Score: 58.5362 bits (140), Expect = 1.380e-25
Identity = 32/87 (36.78%), Postives = 50/87 (57.47%), Query Frame = 3

Query:    6 MVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGI----DFYTTITRARFEELNMDLFRKCMEPVEK 254
            ++N  V+EFK++N  D+  +P AL+RL+ A E+AK+ LSST +T I +  +              TRA+ E L  DL  + +EP +K
Sbjct:  230 ILNFLVEEFKKENGMDLKNDPLALQRLKEAAEKAKKELSSTQETDINLPYITADAAGPKHLNVKFTRAKLESLVSDLIDRTIEPCKK 316

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_BORHD (Chaperone protein dnaK OS=Borrelia hermsii (strain DAH) GN=dnaK PE=2 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 1.380e-25
Identity = 49/109 (44.95%), Postives = 65/109 (59.63%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +  S +++V+LVGGSTRIP +Q+++++ F  +E  K +NPDE           IL+GE     +D             ET GGVMT LI RNTTIPTKK QVFST
Sbjct:  320 KDAGLKASDINEVILVGGSTRIPAIQKIVKEIF-AQEPNKGVNPDEAVAIGAAIQGGILTGE----TKDMVLLDVTPLSLGIETLGGVMTKLIERNTTIPTKKSQVFST 423

HSP 2 Score: 51.2174 bits (121), Expect = 1.380e-25
Identity = 28/84 (33.33%), Postives = 48/84 (57.14%), Query Frame = 3

Query:    6 MVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGID----FYTTITRARFEELNMDLFRKCMEP 245
            ++ H + EFK+ +  D+S +  AL+RL+ A E+AK  LSS  + +I +  +    +       T+TRA+FE++   L +K  EP
Sbjct:  231 IIKHLITEFKKDSAIDLSNDKMALQRLKEAAEKAKIELSSAQEASINLPFITADANGPKHLQYTLTRAKFEQMVDHLVQKTKEP 314

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_RHOPS (Chaperone protein dnaK OS=Rhodopseudomonas palustris (strain BisB5) GN=dnaK PE=2 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 1.380e-25
Identity = 48/109 (44.04%), Postives = 62/109 (56.88%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +    + +VVLVGG TR+PKVQ++++  F GKE  K +NPDE           +L G+    V+D             ET GGV T +I RNTTIPTKK QVFST
Sbjct:  320 KDAGLTAGEIGEVVLVGGMTRMPKVQEVVKQLF-GKEPHKGVNPDEVVAIGAAIQAGVLQGD----VKDVLLLDVTPLSLGIETLGGVFTRIIERNTTIPTKKSQVFST 423

HSP 2 Score: 54.299 bits (129), Expect = 1.380e-25
Identity = 34/88 (38.64%), Postives = 51/88 (57.95%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL---YEGIDFYT-TITRARFEELNMDLFRKCMEPVEK 254
            R+VN+   EF+++   ++  +  AL+RL+ A E+AK  LSST QT I +  +     G    T  +TRA+FE L  DL +K +EP  K
Sbjct:  230 RLVNYLADEFQKEQGINLRNDKLALQRLKEAAEKAKIELSSTTQTEINLPFITADQSGPKHLTMKLTRAKFEALVDDLVQKTIEPCRK 317

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_NITWN (Chaperone protein dnaK OS=Nitrobacter winogradskyi (strain Nb-255 / ATCC 25391) GN=dnaK PE=2 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 1.380e-25
Identity = 48/109 (44.04%), Postives = 62/109 (56.88%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +    + +VVLVGG TR+PKVQ++++  F GKE  K +NPDE           +L G+    V+D             ET GGV T +I RNTTIPTKK QVFST
Sbjct:  320 KDAGLTAGEIGEVVLVGGMTRMPKVQEVVKQLF-GKEPHKGVNPDEVVAIGAAIQAGVLQGD----VKDVLLLDVTPLSLGIETLGGVFTRIIDRNTTIPTKKSQVFST 423

HSP 2 Score: 54.299 bits (129), Expect = 1.380e-25
Identity = 34/88 (38.64%), Postives = 51/88 (57.95%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL---YEGIDFYT-TITRARFEELNMDLFRKCMEPVEK 254
            R+VN+   EF+++   ++  +  AL+RL+ A E+AK  LSST QT I +  +     G    T  +TRA+FE L  DL +K +EP  K
Sbjct:  230 RLVNYLADEFQKEQGINLRNDKLALQRLKEAAEKAKIELSSTTQTEINLPFITADQSGPKHLTMKLTRAKFEALVDDLVQKTIEPCRK 317

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_PEDPA (Chaperone protein dnaK OS=Pediococcus pentosaceus (strain ATCC 25745 / 183-1w) GN=dnaK PE=2 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 1.380e-25
Identity = 48/109 (44.04%), Postives = 62/109 (56.88%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +  S + +V+L GGSTRIP VQ+ ++++  GKE   SINPDE           +L+G+    V+D             ET GGV T LI RNTTIPT K QVFST
Sbjct:  298 KDAGLSASEIDEVILNGGSTRIPAVQEAVKNW-TGKEPNHSINPDEAVALGAAVQGGVLTGD----VKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSKSQVFST 401

HSP 2 Score: 57.3806 bits (137), Expect = 1.380e-25
Identity = 30/87 (34.48%), Postives = 51/87 (58.62%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEG----IDFYTTITRARFEELNMDLFRKCMEPVE 251
            ++++  V EFK+ N  D+S +  AL+RL+ A E+AK+ LS  ++  I +  +  G    +    T+TRA+F++L  DL  +   PVE
Sbjct:  208 KIIDWLVDEFKKDNGVDLSQDKMALQRLKDAAEKAKKDLSGVSEAQISLPFISAGEAGPLHLEKTLTRAQFDQLTADLVERTKAPVE 294

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_STRGR (Chaperone protein dnaK OS=Streptomyces griseus GN=dnaK PE=2 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 1.380e-25
Identity = 45/109 (41.28%), Postives = 62/109 (56.88%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +  S +  VVLVGGSTR+P V +L+++   G+E  K +NPDE           +L GE    V+D             ET GG+MT LI RNTTIPTK+ ++F+T
Sbjct:  295 KDAGIQLSEIDHVVLVGGSTRMPAVAELVKELTGGQEANKGVNPDEVVAIGASLQAGVLKGE----VKDVLLLDVTPLSLGIETKGGIMTKLIERNTTIPTKRSEIFTT 399

HSP 2 Score: 53.9138 bits (128), Expect = 1.380e-25
Identity = 32/85 (37.65%), Postives = 52/85 (61.18%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIE---IDSLYEG-IDFYTTITRARFEELNMDLFRKCMEP 245
            R+V++ V++F   +  D+S +  AL+RLR A E+AK  LSS+ +TTI    I +  EG +     +TR++F++L  DL  +C  P
Sbjct:  205 RVVDYLVKQFANGHGVDLSKDKMALQRLREAAEKAKIELSSSTETTINLPYITASAEGPLHLDEKLTRSQFQQLTADLLDRCKTP 289

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_STRGG (Chaperone protein dnaK OS=Streptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350) GN=dnaK PE=2 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 1.380e-25
Identity = 45/109 (41.28%), Postives = 62/109 (56.88%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +  S +  VVLVGGSTR+P V +L+++   G+E  K +NPDE           +L GE    V+D             ET GG+MT LI RNTTIPTK+ ++F+T
Sbjct:  295 KDAGIQLSEIDHVVLVGGSTRMPAVAELVKELTGGQEANKGVNPDEVVAIGASLQAGVLKGE----VKDVLLLDVTPLSLGIETKGGIMTKLIERNTTIPTKRSEIFTT 399

HSP 2 Score: 53.9138 bits (128), Expect = 1.380e-25
Identity = 32/85 (37.65%), Postives = 52/85 (61.18%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIE---IDSLYEG-IDFYTTITRARFEELNMDLFRKCMEP 245
            R+V++ V++F   +  D+S +  AL+RLR A E+AK  LSS+ +TTI    I +  EG +     +TR++F++L  DL  +C  P
Sbjct:  205 RVVDYLVKQFANGHGVDLSKDKMALQRLREAAEKAKIELSSSTETTINLPYITASAEGPLHLDEKLTRSQFQQLTADLLDRCKTP 289

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_RHOBA (Chaperone protein dnaK OS=Rhodopirellula baltica GN=dnaK PE=2 SV=2)

HSP 1 Score: 83.9593 bits (206), Expect = 1.795e-25
Identity = 46/109 (42.20%), Postives = 64/109 (58.72%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA MD   + +VVLVGGSTR+PKV+++++  F GK+  + +NPDE           +L+G+ N    D             ET GGVMT L+ RNTTIP +K+ VFST
Sbjct:  329 KDAGMDPKDIDEVVLVGGSTRVPKVREVVKSIF-GKDPHQGVNPDEVVAVGAAIQGSVLAGDRN----DVLLLDVTPLTLGIETEGGVMTALVERNTTIPAEKKNVFST 432

HSP 2 Score: 52.7582 bits (125), Expect = 1.795e-25
Identity = 31/85 (36.47%), Postives = 49/85 (57.65%), Query Frame = 3

Query:    6 MVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL---YEGIDFYT-TITRARFEELNMDLFRKCMEPV 248
            ++N+   EF++ N  D+  +  AL+RL+ ACE+AK+ LS+  +T I +  +     G    T  ITR++FEEL   L  +C  PV
Sbjct:  240 LINYVASEFQKDNGIDLRNDAMALQRLQEACEKAKKELSTLPETDINLPFITMDASGPKHLTMKITRSKFEELIDALVERCRGPV 324

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_METNO (Chaperone protein dnaK OS=Methylobacterium nodulans (strain ORS2060 / LMG 21967) GN=dnaK PE=2 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 1.796e-25
Identity = 49/109 (44.95%), Postives = 63/109 (57.80%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +  + + +VVLVGG TR+PK+Q +++ FF GKE  K +NPDE           +L G+    V+D             ET GGV T LI RNTTIPTKK QVFST
Sbjct:  320 KDAGVSANEIDEVVLVGGMTRMPKIQDVVKTFF-GKEPHKGVNPDEVVAIGAAVQAGVLQGD----VKDVLLLDVTPLSLGIETLGGVFTRLIDRNTTIPTKKSQVFST 423

HSP 2 Score: 51.6026 bits (122), Expect = 1.796e-25
Identity = 31/88 (35.23%), Postives = 48/88 (54.55%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGID----FYTTITRARFEELNMDLFRKCMEPVEK 254
            R+V +   EFKR+   D++ +  AL+RL+ A E+AK  LSS  QT I +  +             ++RA+FE L  DL ++ +EP  K
Sbjct:  230 RIVEYLAAEFKREQGIDLTKDKLALQRLKEAAEKAKIELSSATQTEINLPYITADASGPKHLALKLSRAKFESLVDDLIQRTIEPCRK 317

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_JANSC (Chaperone protein dnaK OS=Jannaschia sp. (strain CCS1) GN=dnaK PE=2 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 1.796e-25
Identity = 49/109 (44.95%), Postives = 60/109 (55.05%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA + K  + +VVLVGG TR+PKV   +  FF GKE  K +NPDE           +L G+    V+D             ET GGV T LI RNTTIPTKK Q+FST
Sbjct:  320 KDAGLSKGDIDEVVLVGGMTRMPKVIDEVTKFF-GKEPHKGVNPDEVVAMGAAIQAGVLQGD----VKDVVLLDVTPLSMGIETLGGVFTRLIDRNTTIPTKKSQIFST 423

HSP 2 Score: 54.6842 bits (130), Expect = 1.796e-25
Identity = 31/86 (36.05%), Postives = 51/86 (59.30%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTT-----IEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEP 245
            R+VN+  +EFK++N  D++ +  AL+RL+ A E+AK  LSS +QT      I + S  + +     +TRA+ E L  DL +  ++P
Sbjct:  229 RIVNYLAEEFKKENSVDLTQDKMALQRLKEAAEKAKIELSSASQTEINQPFISMGSNGQPLHMVMKLTRAKLESLVGDLIKASIKP 314

The following BLAST results are available for this feature:

BLAST of CB417297 vs. ExPASy Swiss-Prot

BLAST of CB417297 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
DNAK_EHRSE	1.380e-25	42.20	Chaperone protein dnaK OS=Ehrlichia sennetsu GN=dn...	[more]
DNAK_BORHD	1.380e-25	44.95	Chaperone protein dnaK OS=Borrelia hermsii (strain...	[more]
DNAK_RHOPS	1.380e-25	44.04	Chaperone protein dnaK OS=Rhodopseudomonas palustr...	[more]
DNAK_NITWN	1.380e-25	44.04	Chaperone protein dnaK OS=Nitrobacter winogradskyi...	[more]
DNAK_PEDPA	1.380e-25	44.04	Chaperone protein dnaK OS=Pediococcus pentosaceus ...	[more]
DNAK_STRGR	1.380e-25	41.28	Chaperone protein dnaK OS=Streptomyces griseus GN=...	[more]
DNAK_STRGG	1.380e-25	41.28	Chaperone protein dnaK OS=Streptomyces griseus sub...	[more]
DNAK_RHOBA	1.795e-25	42.20	Chaperone protein dnaK OS=Rhodopirellula baltica G...	[more]
DNAK_METNO	1.796e-25	44.95	Chaperone protein dnaK OS=Methylobacterium nodulan...	[more]
DNAK_JANSC	1.796e-25	44.95	Chaperone protein dnaK OS=Jannaschia sp. (strain C...	[more]

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Properties


Property Name	Value
Genbank description	EST0370 Mature peel (flavedo) cDNA subtraction library Citrus sinensis cDNA clone 6P015 similar to 70 kDa heat shock protein (acc# AAL24367), mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CB417297 ID=CB417297; Name=CB417297; organism=Citrus sinensis; type=EST; length=590bp
ACAGAATGGTGAACCACTTTGTTCAAGAGTTCAAGAGGAAAAACAAGAAG
GATATTAGTGGAAACCCCAGAGCGTTGAGGAGGTTGAGAACTGCCTGTGA
AAGGGCTAAGAGGACCCTGTCATCAACCGCACAGACTACAATTGAGATTG
ATTCTTTATATGAGGGTATTGATTTCTACACAACCATTACAAGGGCTAGA
TTCGAAGAGTTGAACATGGATTTGTTCAGGAAGTGTATGGAGCCAGTGGA
GAAATGCGAGAGATGCTAAGATGGACAAGAGCAGTGTCCATGATGTGGTT
CTTGTTGGTGGGTCTACTAGGATCCCAAAAGTCCAGCAACTGTTGCAGGA
CTTCTTCAATGGAAAAGAGCTATGCAAGAGCATCAACCCTGATGAAGCGG
TTGCCTACGGTGCTGCTGTGCAGGCTGCAATCTTGAGTGGTGAGGGTAAT
GAGAAGGTTCAGGACCTCTTATTGCTTGACGTCACCCCTCTATCGCTGGG
TTTGGAGACTGCTGGTGGAGTCATGACTGTGTTGATTCCAAGGAACACCA
CTATTCCAACCAAGAAGGAGCAGGTGTTCTCCACATACTC