CB417297

Overview
NameCB417297
Unique NameCB417297
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length590
Libraries
Library NameType
Mature peel (flavedo) cDNA subtraction librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_BACFR (Chaperone protein dnaK OS=Bacteroides fragilis GN=dnaK PE=2 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 1.796e-25
Identity = 45/109 (41.28%), Postives = 61/109 (55.96%), Query Frame = 1 
Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +  S + +V+LVGGS+RIP VQ+L++DFF GK   K +NPDE           +L+ E    ++              ET GGVMT LI  NTTIP +K + FST
Sbjct:  319 QDAGLSNSDIDEVILVGGSSRIPAVQKLVEDFF-GKTPSKGVNPDEVVAVGAAVQGAVLTDE----IKGVVLLDVTPLSMGIETLGGVMTKLIDANTTIPARKSETFST 422

HSP 2 Score: 57.7658 bits (138), Expect = 1.796e-25
Identity = 32/87 (36.78%), Postives = 52/87 (59.77%), Query Frame = 3 
Query:    6 MVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYE--GI--DFYTTITRARFEELNMDLFRKCMEPVEK 254
            +++  VQEFK     D++ +P A++RL+ A E+AK  LSS+  T I +  +    G+      T+TRA+FE L  +L + C+EP +K
Sbjct:  230 IIDWLVQEFKNDEGADLTQDPMAMQRLKEAAEKAKIELSSSTSTEINLPYIMPVGGVPKHLVKTLTRAKFESLAHNLIQACLEPCKK 316
[Close]
BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_BACFN (Chaperone protein dnaK OS=Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) GN=dnaK PE=2 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 1.796e-25
Identity = 45/109 (41.28%), Postives = 61/109 (55.96%), Query Frame = 1 
Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +  S + +V+LVGGS+RIP VQ+L++DFF GK   K +NPDE           +L+ E    ++              ET GGVMT LI  NTTIP +K + FST
Sbjct:  319 QDAGLSNSDIDEVILVGGSSRIPAVQKLVEDFF-GKTPSKGVNPDEVVAVGAAVQGAVLTDE----IKGVVLLDVTPLSMGIETLGGVMTKLIDANTTIPARKSETFST 422

HSP 2 Score: 57.7658 bits (138), Expect = 1.796e-25
Identity = 32/87 (36.78%), Postives = 52/87 (59.77%), Query Frame = 3 
Query:    6 MVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYE--GI--DFYTTITRARFEELNMDLFRKCMEPVEK 254
            +++  VQEFK     D++ +P A++RL+ A E+AK  LSS+  T I +  +    G+      T+TRA+FE L  +L + C+EP +K
Sbjct:  230 IIDWLVQEFKNDEGADLTQDPMAMQRLKEAAEKAKIELSSSTSTEINLPYIMPVGGVPKHLVKTLTRAKFESLAHNLIQACLEPCKK 316
[Close]
BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_WOLSU (Chaperone protein dnaK OS=Wolinella succinogenes GN=dnaK PE=2 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.796e-25
Identity = 54/109 (49.54%), Postives = 66/109 (60.55%), Query Frame = 1 
Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA + KS + +VV+VGGSTRIPKVQQ ++DF  GKEL KS+NPDE           +L G+    V+D             ET GGVMT +I R TTIP KK QVFST
Sbjct:  319 KDAGLAKSDIAEVVMVGGSTRIPKVQQRVKDFI-GKELNKSVNPDEVVALGAAIQGGVLKGD----VKDVLLLDVTPLSLGIETLGGVMTKIIDRGTTIPVKKSQVFST 422

HSP 2 Score: 43.5134 bits (101), Expect = 1.796e-25
Identity = 26/87 (29.89%), Postives = 47/87 (54.02%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGI----DFYTTITRARFEELNMDLFRKCMEPVE 251
            R+++   +EF+ +N  D+  +  AL+RL+ A E AK+ LSS  +T I +  +             I+RA+FE L  DL  + ++ ++
Sbjct:  229 RIIDWAAKEFEAENGIDLKKDVMALQRLKDAAENAKKELSSANETEINLPFITADATGPKHLVKKISRAKFESLIDDLIEQTIQKID 315
[Close]
BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_BARBK (Chaperone protein dnaK OS=Bartonella bacilliformis (strain ATCC 35685 / KC583) GN=dnaK PE=2 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 1.796e-25
Identity = 48/109 (44.04%), Postives = 63/109 (57.80%), Query Frame = 1 
Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +    + +VVLVGG TR+PK+Q+++Q+FF GK+  K +NPDE           +L G+    V+D             ET GGV T LI RNTTIPTKK Q FST
Sbjct:  320 KDAGLRAGEIDEVVLVGGMTRMPKIQEVVQNFF-GKDPHKGVNPDEVVAMGAAIQGGVLQGD----VKDVLLLDVTPLSLGIETLGGVFTRLIERNTTIPTKKSQTFST 423

HSP 2 Score: 51.9878 bits (123), Expect = 1.796e-25
Identity = 31/84 (36.90%), Postives = 50/84 (59.52%), Query Frame = 3 
Query:    6 MVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL---YEGIDFYT-TITRARFEELNMDLFRKCMEP 245
            +V++F  EFK++   D+  +  AL+RL+ A E+AK  LSS+ QT + +  +     G    T  +TRA+FE L  DL ++ +EP
Sbjct:  231 LVSYFADEFKKEQGIDLKNDKLALQRLKEAAEKAKIELSSSQQTEVNLPFITADQSGPKHLTMKLTRAKFESLVDDLVQRTIEP 314
[Close]
BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_RICTY (Chaperone protein dnaK OS=Rickettsia typhi GN=dnaK PE=2 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 1.796e-25
Identity = 48/109 (44.04%), Postives = 62/109 (56.88%), Query Frame = 1 
Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA    + + +VVLVGG TR+PKVQ+ ++ FF G+E  K +NPDE           +L    N++V D             ET GGV T LI RNTTIP+KK QVFST
Sbjct:  319 KDAGFKPTDIQEVVLVGGMTRMPKVQEAVKKFF-GREPHKGVNPDEVVALGAAIQGGVL----NKEVTDILLLDVTPLSLGIETLGGVFTRLIDRNTTIPSKKSQVFST 422

HSP 2 Score: 55.0694 bits (131), Expect = 1.796e-25
Identity = 32/85 (37.65%), Postives = 48/85 (56.47%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGI----DFYTTITRARFEELNMDLFRKCMEP 245
            R++NH +  FK+ +  D+S +P AL+RL+ A E+AK+ LSST+ T I +  +              TRA  E+L  DL  K +EP
Sbjct:  229 RILNHLIDVFKKDSGIDLSKDPLALQRLKEAAEKAKKELSSTSATDINLPYITADSTGPKHLNIKFTRAELEKLVDDLIEKTIEP 313
[Close]
BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_RICFE (Chaperone protein dnaK OS=Rickettsia felis GN=dnaK PE=2 SV=2)

HSP 1 Score: 81.6481 bits (200), Expect = 1.796e-25
Identity = 48/109 (44.04%), Postives = 62/109 (56.88%), Query Frame = 1 
Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA    + + +VVLVGG TR+PKVQ+ ++ FF G+E  K +NPDE           +L    N++V D             ET GGV T LI RNTTIP+KK QVFST
Sbjct:  319 KDAGFKPTDIQEVVLVGGMTRMPKVQEAVKKFF-GREPHKGVNPDEVVALGAAIQGGVL----NKEVTDILLLDVTPLSLGIETLGGVFTRLIDRNTTIPSKKSQVFST 422

HSP 2 Score: 55.0694 bits (131), Expect = 1.796e-25
Identity = 32/85 (37.65%), Postives = 48/85 (56.47%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGI----DFYTTITRARFEELNMDLFRKCMEP 245
            R++NH +  FK+ +  D+S +P AL+RL+ A E+AK+ LSST+ T I +  +              TRA  E+L  DL  K +EP
Sbjct:  229 RILNHLIDVFKKDSGIDLSKDPLALQRLKEAAEKAKKELSSTSATDINLPYITADSTGPKHLNIKFTRAELEKLVDDLIEKTIEP 313
[Close]
BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_LACCB (Chaperone protein dnaK OS=Lactobacillus casei (strain BL23) GN=dnaK PE=2 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 1.796e-25
Identity = 46/109 (42.20%), Postives = 60/109 (55.05%), Query Frame = 1 
Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DAK+  + +  V+L GGSTR P VQ+ ++ +  GK+   SINPDE           ++SG+    V+D             ET GGV T LI RNTTIPT K QVFST
Sbjct:  296 KDAKLTNADIDKVILNGGSTRTPAVQEAVKQW-TGKDPDHSINPDEAVALGAAIQGGVISGD----VKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSKSQVFST 399

HSP 2 Score: 59.6918 bits (143), Expect = 1.796e-25
Identity = 32/87 (36.78%), Postives = 51/87 (58.62%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEG----IDFYTTITRARFEELNMDLFRKCMEPVE 251
            ++++  V EFK+ N  D+S +  A++RL+ A E+AK+ LS   QT I +  +  G    +    T+TRA+F+E+  DL  K   PVE
Sbjct:  206 KIIDWLVAEFKKDNNIDLSKDKMAMQRLKDAAEKAKKDLSGVTQTQISLPFISAGPNGPLHLERTLTRAQFDEMTADLVAKTKIPVE 292
[Close]
BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_LACC3 (Chaperone protein dnaK OS=Lactobacillus casei (strain ATCC 334) GN=dnaK PE=2 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 1.796e-25
Identity = 46/109 (42.20%), Postives = 60/109 (55.05%), Query Frame = 1 
Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DAK+  + +  V+L GGSTR P VQ+ ++ +  GK+   SINPDE           ++SG+    V+D             ET GGV T LI RNTTIPT K QVFST
Sbjct:  296 KDAKLTNADIDKVILNGGSTRTPAVQEAVKQW-TGKDPDHSINPDEAVALGAAIQGGVISGD----VKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSKSQVFST 399

HSP 2 Score: 59.6918 bits (143), Expect = 1.796e-25
Identity = 32/87 (36.78%), Postives = 51/87 (58.62%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEG----IDFYTTITRARFEELNMDLFRKCMEPVE 251
            ++++  V EFK+ N  D+S +  A++RL+ A E+AK+ LS   QT I +  +  G    +    T+TRA+F+E+  DL  K   PVE
Sbjct:  206 KIIDWLVAEFKKDNNIDLSKDKMAMQRLKDAAEKAKKDLSGVTQTQISLPFISAGPNGPLHLERTLTRAQFDEMTADLVAKTKIPVE 292
[Close]
BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_CLOK5 (Chaperone protein dnaK OS=Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680) GN=dnaK PE=2 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 1.796e-25
Identity = 49/108 (45.37%), Postives = 62/108 (57.41%), Query Frame = 1 
Query:  262 DAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            DA +  + ++ V+LVGGSTRIP VQ+ ++ F  GKE  K +NPDE           +L+G+    V+D             ET GGV T LI RNTTIPTKK QVFST
Sbjct:  298 DAGLTINDINKVILVGGSTRIPAVQEAVKSF-TGKEPSKGVNPDECVAMGAAIQAGVLTGD----VKDVLLLDVTPLTLGIETLGGVATPLIERNTTIPTKKSQVFST 400

HSP 2 Score: 53.5286 bits (127), Expect = 1.796e-25
Identity = 31/90 (34.44%), Postives = 52/90 (57.78%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLY------EGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            +++++    FK  N  D+  +  AL+RL+ A E+AK  LSS+ QT I +  +       + ID   ++TRA+F EL  DL  + +EP++K
Sbjct:  207 KIIDYIADTFKADNGIDLKNDKMALQRLKEAAEKAKIELSSSTQTNINLPFITADATGPKHIDM--SLTRAKFNELTQDLVDRTIEPMKK 294
[Close]
BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_CLOK1 (Chaperone protein dnaK OS=Clostridium kluyveri (strain NBRC 12016) GN=dnaK PE=2 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 1.796e-25
Identity = 49/108 (45.37%), Postives = 62/108 (57.41%), Query Frame = 1 
Query:  262 DAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            DA +  + ++ V+LVGGSTRIP VQ+ ++ F  GKE  K +NPDE           +L+G+    V+D             ET GGV T LI RNTTIPTKK QVFST
Sbjct:  298 DAGLTINDINKVILVGGSTRIPAVQEAVKSF-TGKEPSKGVNPDECVAMGAAIQAGVLTGD----VKDVLLLDVTPLTLGIETLGGVATPLIERNTTIPTKKSQVFST 400

HSP 2 Score: 53.5286 bits (127), Expect = 1.796e-25
Identity = 31/90 (34.44%), Postives = 52/90 (57.78%), Query Frame = 3 
Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLY------EGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            +++++    FK  N  D+  +  AL+RL+ A E+AK  LSS+ QT I +  +       + ID   ++TRA+F EL  DL  + +EP++K
Sbjct:  207 KIIDYIADTFKADNGIDLKNDKMALQRLKEAAEKAKIELSSSTQTNINLPFITADATGPKHIDM--SLTRAKFNELTQDLVDRTIEPMKK 294
The following BLAST results are available for this feature:
BLAST of CB417297 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
DNAK_BACFR1.796e-2541.28Chaperone protein dnaK OS=Bacteroides fragilis GN=... [more]
DNAK_BACFN1.796e-2541.28Chaperone protein dnaK OS=Bacteroides fragilis (st... [more]
DNAK_WOLSU1.796e-2549.54Chaperone protein dnaK OS=Wolinella succinogenes G... [more]
DNAK_BARBK1.796e-2544.04Chaperone protein dnaK OS=Bartonella bacilliformis... [more]
DNAK_RICTY1.796e-2544.04Chaperone protein dnaK OS=Rickettsia typhi GN=dnaK... [more]
DNAK_RICFE1.796e-2544.04Chaperone protein dnaK OS=Rickettsia felis GN=dnaK... [more]
DNAK_LACCB1.796e-2542.20Chaperone protein dnaK OS=Lactobacillus casei (str... [more]
DNAK_LACC31.796e-2542.20Chaperone protein dnaK OS=Lactobacillus casei (str... [more]
DNAK_CLOK51.796e-2545.37Chaperone protein dnaK OS=Clostridium kluyveri (st... [more]
DNAK_CLOK11.796e-2545.37Chaperone protein dnaK OS=Clostridium kluyveri (st... [more]

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Properties
Property NameValue
Genbank descriptionEST0370 Mature peel (flavedo) cDNA subtraction library Citrus sinensis cDNA clone 6P015 similar to 70 kDa heat shock protein (acc# AAL24367), mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CB417297 ID=CB417297; Name=CB417297; organism=Citrus sinensis; type=EST; length=590bp
ACAGAATGGTGAACCACTTTGTTCAAGAGTTCAAGAGGAAAAACAAGAAG
GATATTAGTGGAAACCCCAGAGCGTTGAGGAGGTTGAGAACTGCCTGTGA
AAGGGCTAAGAGGACCCTGTCATCAACCGCACAGACTACAATTGAGATTG
ATTCTTTATATGAGGGTATTGATTTCTACACAACCATTACAAGGGCTAGA
TTCGAAGAGTTGAACATGGATTTGTTCAGGAAGTGTATGGAGCCAGTGGA
GAAATGCGAGAGATGCTAAGATGGACAAGAGCAGTGTCCATGATGTGGTT
CTTGTTGGTGGGTCTACTAGGATCCCAAAAGTCCAGCAACTGTTGCAGGA
CTTCTTCAATGGAAAAGAGCTATGCAAGAGCATCAACCCTGATGAAGCGG
TTGCCTACGGTGCTGCTGTGCAGGCTGCAATCTTGAGTGGTGAGGGTAAT
GAGAAGGTTCAGGACCTCTTATTGCTTGACGTCACCCCTCTATCGCTGGG
TTTGGAGACTGCTGGTGGAGTCATGACTGTGTTGATTCCAAGGAACACCA
CTATTCCAACCAAGAAGGAGCAGGTGTTCTCCACATACTC
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