CB417297 | Citrus Genome Database

Overview


Name	CB417297
Unique Name	CB417297
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	590

Libraries


Library Name	Type
Mature peel (flavedo) cDNA subtraction library	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig13846	contig	Csv1_Contig13846:800..1391.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_STAAW (Chaperone protein dnaK OS=Staphylococcus aureus (strain MW2) GN=dnaK PE=2 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 1.797e-25
Identity = 43/109 (39.45%), Postives = 59/109 (54.13%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +  S + +V+LVGGSTRIP VQ+ ++    GKE  K +NPDE           +++G+    V+D             E  GG M  LI RNTTIPT K Q++ST
Sbjct:  295 KDAGLTNSDIDEVILVGGSTRIPAVQEAVKKEI-GKEPNKGVNPDEVVAMGAAIQGGVITGD----VKDVVLLDVTPLSLGIEILGGRMNTLIERNTTIPTSKSQIYST 398

HSP 2 Score: 62.7734 bits (151), Expect = 1.797e-25
Identity = 32/87 (36.78%), Postives = 54/87 (62.07%), Query Frame = 3

Query:    6 MVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEG----IDFYTTITRARFEELNMDLFRKCMEPVEK 254
            ++++ V EFK++N  D+S +  AL+RL+ A E+AK+ LS  +QT I +  +  G    +     +TR++FEEL+  L R+ MEP  +
Sbjct:  206 IIDYLVAEFKKENGVDLSQDKMALQRLKDAAEKAKKDLSGVSQTQISLPFISAGENGPLHLEVNLTRSKFEELSDSLIRRTMEPTRQ 292

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_STAAU (Chaperone protein dnaK OS=Staphylococcus aureus GN=dnaK PE=2 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 1.797e-25
Identity = 43/109 (39.45%), Postives = 59/109 (54.13%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +  S + +V+LVGGSTRIP VQ+ ++    GKE  K +NPDE           +++G+    V+D             E  GG M  LI RNTTIPT K Q++ST
Sbjct:  295 KDAGLTNSDIDEVILVGGSTRIPAVQEAVKKEI-GKEPNKGVNPDEVVAMGAAIQGGVITGD----VKDVVLLDVTPLSLGIEILGGRMNTLIERNTTIPTSKSQIYST 398

HSP 2 Score: 62.7734 bits (151), Expect = 1.797e-25
Identity = 32/87 (36.78%), Postives = 54/87 (62.07%), Query Frame = 3

Query:    6 MVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEG----IDFYTTITRARFEELNMDLFRKCMEPVEK 254
            ++++ V EFK++N  D+S +  AL+RL+ A E+AK+ LS  +QT I +  +  G    +     +TR++FEEL+  L R+ MEP  +
Sbjct:  206 IIDYLVAEFKKENGVDLSQDKMALQRLKDAAEKAKKDLSGVSQTQISLPFISAGENGPLHLEVNLTRSKFEELSDSLIRRTMEPTRQ 292

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_STAAT (Chaperone protein dnaK OS=Staphylococcus aureus (strain USA300 / TCH1516) GN=dnaK PE=2 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 1.797e-25
Identity = 43/109 (39.45%), Postives = 59/109 (54.13%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +  S + +V+LVGGSTRIP VQ+ ++    GKE  K +NPDE           +++G+    V+D             E  GG M  LI RNTTIPT K Q++ST
Sbjct:  295 KDAGLTNSDIDEVILVGGSTRIPAVQEAVKKEI-GKEPNKGVNPDEVVAMGAAIQGGVITGD----VKDVVLLDVTPLSLGIEILGGRMNTLIERNTTIPTSKSQIYST 398

HSP 2 Score: 62.7734 bits (151), Expect = 1.797e-25
Identity = 32/87 (36.78%), Postives = 54/87 (62.07%), Query Frame = 3

Query:    6 MVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEG----IDFYTTITRARFEELNMDLFRKCMEPVEK 254
            ++++ V EFK++N  D+S +  AL+RL+ A E+AK+ LS  +QT I +  +  G    +     +TR++FEEL+  L R+ MEP  +
Sbjct:  206 IIDYLVAEFKKENGVDLSQDKMALQRLKDAAEKAKKDLSGVSQTQISLPFISAGENGPLHLEVNLTRSKFEELSDSLIRRTMEPTRQ 292

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_STAAS (Chaperone protein dnaK OS=Staphylococcus aureus (strain MSSA476) GN=dnaK PE=2 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 1.797e-25
Identity = 43/109 (39.45%), Postives = 59/109 (54.13%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +  S + +V+LVGGSTRIP VQ+ ++    GKE  K +NPDE           +++G+    V+D             E  GG M  LI RNTTIPT K Q++ST
Sbjct:  295 KDAGLTNSDIDEVILVGGSTRIPAVQEAVKKEI-GKEPNKGVNPDEVVAMGAAIQGGVITGD----VKDVVLLDVTPLSLGIEILGGRMNTLIERNTTIPTSKSQIYST 398

HSP 2 Score: 62.7734 bits (151), Expect = 1.797e-25
Identity = 32/87 (36.78%), Postives = 54/87 (62.07%), Query Frame = 3

Query:    6 MVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEG----IDFYTTITRARFEELNMDLFRKCMEPVEK 254
            ++++ V EFK++N  D+S +  AL+RL+ A E+AK+ LS  +QT I +  +  G    +     +TR++FEEL+  L R+ MEP  +
Sbjct:  206 IIDYLVAEFKKENGVDLSQDKMALQRLKDAAEKAKKDLSGVSQTQISLPFISAGENGPLHLEVNLTRSKFEELSDSLIRRTMEPTRQ 292

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_STAAR (Chaperone protein dnaK OS=Staphylococcus aureus (strain MRSA252) GN=dnaK PE=2 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 1.797e-25
Identity = 43/109 (39.45%), Postives = 59/109 (54.13%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +  S + +V+LVGGSTRIP VQ+ ++    GKE  K +NPDE           +++G+    V+D             E  GG M  LI RNTTIPT K Q++ST
Sbjct:  295 KDAGLTNSDIDEVILVGGSTRIPAVQEAVKKEI-GKEPNKGVNPDEVVAMGAAIQGGVITGD----VKDVVLLDVTPLSLGIEILGGRMNTLIERNTTIPTSKSQIYST 398

HSP 2 Score: 62.7734 bits (151), Expect = 1.797e-25
Identity = 32/87 (36.78%), Postives = 54/87 (62.07%), Query Frame = 3

Query:    6 MVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEG----IDFYTTITRARFEELNMDLFRKCMEPVEK 254
            ++++ V EFK++N  D+S +  AL+RL+ A E+AK+ LS  +QT I +  +  G    +     +TR++FEEL+  L R+ MEP  +
Sbjct:  206 IIDYLVAEFKKENGVDLSQDKMALQRLKDAAEKAKKDLSGVSQTQISLPFISAGENGPLHLEVNLTRSKFEELSDSLIRRTMEPTRQ 292

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_STAAN (Chaperone protein dnaK OS=Staphylococcus aureus (strain N315) GN=dnaK PE=1 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 1.797e-25
Identity = 43/109 (39.45%), Postives = 59/109 (54.13%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +  S + +V+LVGGSTRIP VQ+ ++    GKE  K +NPDE           +++G+    V+D             E  GG M  LI RNTTIPT K Q++ST
Sbjct:  295 KDAGLTNSDIDEVILVGGSTRIPAVQEAVKKEI-GKEPNKGVNPDEVVAMGAAIQGGVITGD----VKDVVLLDVTPLSLGIEILGGRMNTLIERNTTIPTSKSQIYST 398

HSP 2 Score: 62.7734 bits (151), Expect = 1.797e-25
Identity = 32/87 (36.78%), Postives = 54/87 (62.07%), Query Frame = 3

Query:    6 MVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEG----IDFYTTITRARFEELNMDLFRKCMEPVEK 254
            ++++ V EFK++N  D+S +  AL+RL+ A E+AK+ LS  +QT I +  +  G    +     +TR++FEEL+  L R+ MEP  +
Sbjct:  206 IIDYLVAEFKKENGVDLSQDKMALQRLKDAAEKAKKDLSGVSQTQISLPFISAGENGPLHLEVNLTRSKFEELSDSLIRRTMEPTRQ 292

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_STAAM (Chaperone protein dnaK OS=Staphylococcus aureus (strain Mu50 / ATCC 700699) GN=dnaK PE=1 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 1.797e-25
Identity = 43/109 (39.45%), Postives = 59/109 (54.13%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +  S + +V+LVGGSTRIP VQ+ ++    GKE  K +NPDE           +++G+    V+D             E  GG M  LI RNTTIPT K Q++ST
Sbjct:  295 KDAGLTNSDIDEVILVGGSTRIPAVQEAVKKEI-GKEPNKGVNPDEVVAMGAAIQGGVITGD----VKDVVLLDVTPLSLGIEILGGRMNTLIERNTTIPTSKSQIYST 398

HSP 2 Score: 62.7734 bits (151), Expect = 1.797e-25
Identity = 32/87 (36.78%), Postives = 54/87 (62.07%), Query Frame = 3

Query:    6 MVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEG----IDFYTTITRARFEELNMDLFRKCMEPVEK 254
            ++++ V EFK++N  D+S +  AL+RL+ A E+AK+ LS  +QT I +  +  G    +     +TR++FEEL+  L R+ MEP  +
Sbjct:  206 IIDYLVAEFKKENGVDLSQDKMALQRLKDAAEKAKKDLSGVSQTQISLPFISAGENGPLHLEVNLTRSKFEELSDSLIRRTMEPTRQ 292

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_STAAE (Chaperone protein dnaK OS=Staphylococcus aureus (strain Newman) GN=dnaK PE=2 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 1.797e-25
Identity = 43/109 (39.45%), Postives = 59/109 (54.13%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +  S + +V+LVGGSTRIP VQ+ ++    GKE  K +NPDE           +++G+    V+D             E  GG M  LI RNTTIPT K Q++ST
Sbjct:  295 KDAGLTNSDIDEVILVGGSTRIPAVQEAVKKEI-GKEPNKGVNPDEVVAMGAAIQGGVITGD----VKDVVLLDVTPLSLGIEILGGRMNTLIERNTTIPTSKSQIYST 398

HSP 2 Score: 62.7734 bits (151), Expect = 1.797e-25
Identity = 32/87 (36.78%), Postives = 54/87 (62.07%), Query Frame = 3

Query:    6 MVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEG----IDFYTTITRARFEELNMDLFRKCMEPVEK 254
            ++++ V EFK++N  D+S +  AL+RL+ A E+AK+ LS  +QT I +  +  G    +     +TR++FEEL+  L R+ MEP  +
Sbjct:  206 IIDYLVAEFKKENGVDLSQDKMALQRLKDAAEKAKKDLSGVSQTQISLPFISAGENGPLHLEVNLTRSKFEELSDSLIRRTMEPTRQ 292

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_STAAC (Chaperone protein dnaK OS=Staphylococcus aureus (strain COL) GN=dnaK PE=2 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 1.797e-25
Identity = 43/109 (39.45%), Postives = 59/109 (54.13%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +  S + +V+LVGGSTRIP VQ+ ++    GKE  K +NPDE           +++G+    V+D             E  GG M  LI RNTTIPT K Q++ST
Sbjct:  295 KDAGLTNSDIDEVILVGGSTRIPAVQEAVKKEI-GKEPNKGVNPDEVVAMGAAIQGGVITGD----VKDVVLLDVTPLSLGIEILGGRMNTLIERNTTIPTSKSQIYST 398

HSP 2 Score: 62.7734 bits (151), Expect = 1.797e-25
Identity = 32/87 (36.78%), Postives = 54/87 (62.07%), Query Frame = 3

Query:    6 MVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEG----IDFYTTITRARFEELNMDLFRKCMEPVEK 254
            ++++ V EFK++N  D+S +  AL+RL+ A E+AK+ LS  +QT I +  +  G    +     +TR++FEEL+  L R+ MEP  +
Sbjct:  206 IIDYLVAEFKKENGVDLSQDKMALQRLKDAAEKAKKDLSGVSQTQISLPFISAGENGPLHLEVNLTRSKFEELSDSLIRRTMEPTRQ 292

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: DNAK_STAA9 (Chaperone protein dnaK OS=Staphylococcus aureus (strain JH9) GN=dnaK PE=2 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 1.797e-25
Identity = 43/109 (39.45%), Postives = 59/109 (54.13%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFST 585
            +DA +  S + +V+LVGGSTRIP VQ+ ++    GKE  K +NPDE           +++G+    V+D             E  GG M  LI RNTTIPT K Q++ST
Sbjct:  295 KDAGLTNSDIDEVILVGGSTRIPAVQEAVKKEI-GKEPNKGVNPDEVVAMGAAIQGGVITGD----VKDVVLLDVTPLSLGIEILGGRMNTLIERNTTIPTSKSQIYST 398

HSP 2 Score: 62.7734 bits (151), Expect = 1.797e-25
Identity = 32/87 (36.78%), Postives = 54/87 (62.07%), Query Frame = 3

Query:    6 MVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEG----IDFYTTITRARFEELNMDLFRKCMEPVEK 254
            ++++ V EFK++N  D+S +  AL+RL+ A E+AK+ LS  +QT I +  +  G    +     +TR++FEEL+  L R+ MEP  +
Sbjct:  206 IIDYLVAEFKKENGVDLSQDKMALQRLKDAAEKAKKDLSGVSQTQISLPFISAGENGPLHLEVNLTRSKFEELSDSLIRRTMEPTRQ 292

The following BLAST results are available for this feature:

BLAST of CB417297 vs. ExPASy Swiss-Prot

BLAST of CB417297 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
DNAK_STAAW	1.797e-25	39.45	Chaperone protein dnaK OS=Staphylococcus aureus (s...	[more]
DNAK_STAAU	1.797e-25	39.45	Chaperone protein dnaK OS=Staphylococcus aureus GN...	[more]
DNAK_STAAT	1.797e-25	39.45	Chaperone protein dnaK OS=Staphylococcus aureus (s...	[more]
DNAK_STAAS	1.797e-25	39.45	Chaperone protein dnaK OS=Staphylococcus aureus (s...	[more]
DNAK_STAAR	1.797e-25	39.45	Chaperone protein dnaK OS=Staphylococcus aureus (s...	[more]
DNAK_STAAN	1.797e-25	39.45	Chaperone protein dnaK OS=Staphylococcus aureus (s...	[more]
DNAK_STAAM	1.797e-25	39.45	Chaperone protein dnaK OS=Staphylococcus aureus (s...	[more]
DNAK_STAAE	1.797e-25	39.45	Chaperone protein dnaK OS=Staphylococcus aureus (s...	[more]
DNAK_STAAC	1.797e-25	39.45	Chaperone protein dnaK OS=Staphylococcus aureus (s...	[more]
DNAK_STAA9	1.797e-25	39.45	Chaperone protein dnaK OS=Staphylococcus aureus (s...	[more]

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Properties


Property Name	Value
Genbank description	EST0370 Mature peel (flavedo) cDNA subtraction library Citrus sinensis cDNA clone 6P015 similar to 70 kDa heat shock protein (acc# AAL24367), mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CB417297 ID=CB417297; Name=CB417297; organism=Citrus sinensis; type=EST; length=590bp
ACAGAATGGTGAACCACTTTGTTCAAGAGTTCAAGAGGAAAAACAAGAAG
GATATTAGTGGAAACCCCAGAGCGTTGAGGAGGTTGAGAACTGCCTGTGA
AAGGGCTAAGAGGACCCTGTCATCAACCGCACAGACTACAATTGAGATTG
ATTCTTTATATGAGGGTATTGATTTCTACACAACCATTACAAGGGCTAGA
TTCGAAGAGTTGAACATGGATTTGTTCAGGAAGTGTATGGAGCCAGTGGA
GAAATGCGAGAGATGCTAAGATGGACAAGAGCAGTGTCCATGATGTGGTT
CTTGTTGGTGGGTCTACTAGGATCCCAAAAGTCCAGCAACTGTTGCAGGA
CTTCTTCAATGGAAAAGAGCTATGCAAGAGCATCAACCCTGATGAAGCGG
TTGCCTACGGTGCTGCTGTGCAGGCTGCAATCTTGAGTGGTGAGGGTAAT
GAGAAGGTTCAGGACCTCTTATTGCTTGACGTCACCCCTCTATCGCTGGG
TTTGGAGACTGCTGGTGGAGTCATGACTGTGTTGATTCCAAGGAACACCA
CTATTCCAACCAAGAAGGAGCAGGTGTTCTCCACATACTC