CF417146 | Citrus Genome Database

Overview


Name	CF417146
Unique Name	CF417146
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	679

Libraries


Library Name	Type
Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

BLAST of CF417146 vs. ExPASy Swiss-Prot
Match: RAB2A_MOUSE (Ras-related protein Rab-2A OS=Mus musculus GN=Rab2a PE=1 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 6.109e-40
Identity = 62/118 (52.54%), Postives = 88/118 (74.58%), Query Frame = 1

Query:  157 YDYLFKLLLIGDSGVGKSCLLLRFADDSYIESYISTIGVDFKIRTVEQDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYASDNVNKLLVGKQ 510
            Y YLFK ++IGD+GVGKSCLLL+F D  +   +  TIGV+F  R +  DGK IKLQIWDTAGQE FR+IT SYYRGA G ++VYD+T +++FN++  WL +  ++++ N+  +L+G +
Sbjct:    3 YAYLFKYIIIGDTGVGKSCLLLQFTDKRFQPVHDLTIGVEFGARMITIDGKQIKLQIWDTAGQESFRSITRSYYRGAAGALLVYDITRRDTFNHLTTWLEDARQHSNSNMVIMLIGNK 120

HSP 2 Score: 50.0618 bits (118), Expect = 6.109e-40
Identity = 25/55 (45.45%), Postives = 37/55 (67.27%), Query Frame = 2

Query:  500 LXNKCDLTANKXVSYETAKAFADEIGIPFMETSAKDSTNVEQAFMAMAASIKDRM 664
            + NK DL + + V  E  +AFA E G+ FMETSAK ++NVE+AF+  A  I +++
Sbjct:  117 IGNKSDLESRREVKKEEGEAFAREHGLIFMETSAKTASNVEEAFINTAKEIYEKI 171

BLAST of CF417146 vs. ExPASy Swiss-Prot
Match: RAB2A_MACFA (Ras-related protein Rab-2A OS=Macaca fascicularis GN=RAB2A PE=2 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 6.109e-40
Identity = 62/118 (52.54%), Postives = 88/118 (74.58%), Query Frame = 1

Query:  157 YDYLFKLLLIGDSGVGKSCLLLRFADDSYIESYISTIGVDFKIRTVEQDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYASDNVNKLLVGKQ 510
            Y YLFK ++IGD+GVGKSCLLL+F D  +   +  TIGV+F  R +  DGK IKLQIWDTAGQE FR+IT SYYRGA G ++VYD+T +++FN++  WL +  ++++ N+  +L+G +
Sbjct:    3 YAYLFKYIIIGDTGVGKSCLLLQFTDKRFQPVHDLTIGVEFGARMITIDGKQIKLQIWDTAGQESFRSITRSYYRGAAGALLVYDITRRDTFNHLTTWLEDARQHSNSNMVIMLIGNK 120

HSP 2 Score: 50.0618 bits (118), Expect = 6.109e-40
Identity = 25/55 (45.45%), Postives = 37/55 (67.27%), Query Frame = 2

Query:  500 LXNKCDLTANKXVSYETAKAFADEIGIPFMETSAKDSTNVEQAFMAMAASIKDRM 664
            + NK DL + + V  E  +AFA E G+ FMETSAK ++NVE+AF+  A  I +++
Sbjct:  117 IGNKSDLESRREVKKEEGEAFAREHGLIFMETSAKTASNVEEAFINTAKEIYEKI 171

BLAST of CF417146 vs. ExPASy Swiss-Prot
Match: RAB2A_HUMAN (Ras-related protein Rab-2A OS=Homo sapiens GN=RAB2A PE=1 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 6.109e-40
Identity = 62/118 (52.54%), Postives = 88/118 (74.58%), Query Frame = 1

Query:  157 YDYLFKLLLIGDSGVGKSCLLLRFADDSYIESYISTIGVDFKIRTVEQDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYASDNVNKLLVGKQ 510
            Y YLFK ++IGD+GVGKSCLLL+F D  +   +  TIGV+F  R +  DGK IKLQIWDTAGQE FR+IT SYYRGA G ++VYD+T +++FN++  WL +  ++++ N+  +L+G +
Sbjct:    3 YAYLFKYIIIGDTGVGKSCLLLQFTDKRFQPVHDLTIGVEFGARMITIDGKQIKLQIWDTAGQESFRSITRSYYRGAAGALLVYDITRRDTFNHLTTWLEDARQHSNSNMVIMLIGNK 120

HSP 2 Score: 50.0618 bits (118), Expect = 6.109e-40
Identity = 25/55 (45.45%), Postives = 37/55 (67.27%), Query Frame = 2

Query:  500 LXNKCDLTANKXVSYETAKAFADEIGIPFMETSAKDSTNVEQAFMAMAASIKDRM 664
            + NK DL + + V  E  +AFA E G+ FMETSAK ++NVE+AF+  A  I +++
Sbjct:  117 IGNKSDLESRREVKKEEGEAFAREHGLIFMETSAKTASNVEEAFINTAKEIYEKI 171

BLAST of CF417146 vs. ExPASy Swiss-Prot
Match: RAB2A_CHICK (Ras-related protein Rab-2A OS=Gallus gallus GN=RAB2A PE=2 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 6.109e-40
Identity = 62/118 (52.54%), Postives = 88/118 (74.58%), Query Frame = 1

Query:  157 YDYLFKLLLIGDSGVGKSCLLLRFADDSYIESYISTIGVDFKIRTVEQDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYASDNVNKLLVGKQ 510
            Y YLFK ++IGD+GVGKSCLLL+F D  +   +  TIGV+F  R +  DGK IKLQIWDTAGQE FR+IT SYYRGA G ++VYD+T +++FN++  WL +  ++++ N+  +L+G +
Sbjct:    3 YAYLFKYIIIGDTGVGKSCLLLQFTDKRFQPVHDLTIGVEFGARMITIDGKQIKLQIWDTAGQESFRSITRSYYRGAAGALLVYDITRRDTFNHLTTWLEDARQHSNSNMVIMLIGNK 120

HSP 2 Score: 50.0618 bits (118), Expect = 6.109e-40
Identity = 25/55 (45.45%), Postives = 37/55 (67.27%), Query Frame = 2

Query:  500 LXNKCDLTANKXVSYETAKAFADEIGIPFMETSAKDSTNVEQAFMAMAASIKDRM 664
            + NK DL + + V  E  +AFA E G+ FMETSAK ++NVE+AF+  A  I +++
Sbjct:  117 IGNKSDLESRREVKKEEGEAFAREHGLIFMETSAKTASNVEEAFINTAKEIYEKI 171

BLAST of CF417146 vs. ExPASy Swiss-Prot
Match: RAB2A_CANFA (Ras-related protein Rab-2A OS=Canis familiaris GN=RAB2A PE=1 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 6.109e-40
Identity = 62/118 (52.54%), Postives = 88/118 (74.58%), Query Frame = 1

Query:  157 YDYLFKLLLIGDSGVGKSCLLLRFADDSYIESYISTIGVDFKIRTVEQDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYASDNVNKLLVGKQ 510
            Y YLFK ++IGD+GVGKSCLLL+F D  +   +  TIGV+F  R +  DGK IKLQIWDTAGQE FR+IT SYYRGA G ++VYD+T +++FN++  WL +  ++++ N+  +L+G +
Sbjct:    3 YAYLFKYIIIGDTGVGKSCLLLQFTDKRFQPVHDLTIGVEFGARMITIDGKQIKLQIWDTAGQESFRSITRSYYRGAAGALLVYDITRRDTFNHLTTWLEDARQHSNSNMVIMLIGNK 120

HSP 2 Score: 50.0618 bits (118), Expect = 6.109e-40
Identity = 25/55 (45.45%), Postives = 37/55 (67.27%), Query Frame = 2

Query:  500 LXNKCDLTANKXVSYETAKAFADEIGIPFMETSAKDSTNVEQAFMAMAASIKDRM 664
            + NK DL + + V  E  +AFA E G+ FMETSAK ++NVE+AF+  A  I +++
Sbjct:  117 IGNKSDLESRREVKKEEGEAFAREHGLIFMETSAKTASNVEEAFINTAKEIYEKI 171

BLAST of CF417146 vs. ExPASy Swiss-Prot
Match: RAA2C_ARATH (Ras-related protein RABA2c OS=Arabidopsis thaliana GN=RABA2C PE=2 SV=4)

HSP 1 Score: 142.51 bits (358), Expect = 7.955e-40
Identity = 66/122 (54.10%), Postives = 92/122 (75.41%), Query Frame = 1

Query:  145 MNPEYDYLFKLLLIGDSGVGKSCLLLRFADDSYIESYISTIGVDFKIRTVEQDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYASDNVNKLLVGKQ 510
            ++ EYDYLFK++LIGDSGVGKS +L RF  + +     STIGV+F  RT + +GKTIK QIWDTAGQER+R ITS+YYRGA G ++VYD+T +++F+NV +WL E+  +A  N+  ++ G +
Sbjct:    5 VDQEYDYLFKIVLIGDSGVGKSNILSRFTRNEFCLESKSTIGVEFATRTTQVEGKTIKAQIWDTAGQERYRAITSAYYRGAVGALLVYDITKRQTFDNVLRWLRELRDHADSNIVIMMAGNK 126

HSP 2 Score: 42.3578 bits (98), Expect = 7.955e-40
Identity = 20/49 (40.82%), Postives = 32/49 (65.31%), Query Frame = 2

Query:  506 NKCDLTANKXVSYETAKAFADEIGIPFMETSAKDSTNVEQAFMAMAASI 652
            NK DL   + V+ E  ++ A++ G+ F+ETSA ++TNVE+AF  +   I
Sbjct:  125 NKSDLNHLRSVAEEDGQSLAEKEGLSFLETSALEATNVEKAFQTILGEI 173

BLAST of CF417146 vs. ExPASy Swiss-Prot
Match: RAB43_HUMAN (Ras-related protein Rab-43 OS=Homo sapiens GN=RAB43 PE=1 SV=1)

HSP 1 Score: 145.206 bits (365), Expect = 7.959e-40
Identity = 64/119 (53.78%), Postives = 91/119 (76.47%), Query Frame = 1

Query:  154 EYDYLFKLLLIGDSGVGKSCLLLRFADDSYIESYISTIGVDFKIRTVEQDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYASDNVNKLLVGKQ 510
            +YD+LFKL+L+GD+ VGK+C++ RF   ++ E   STIGVDF ++T+E  GK +KLQIWDTAGQERFRTIT SYYR A+G I+ YD+T + SF +V  W+ ++ +YA  N+ +LL+G +
Sbjct:   14 QYDFLFKLVLVGDASVGKTCVVQRFKTGAFSERQGSTIGVDFTMKTLEIQGKRVKLQIWDTAGQERFRTITQSYYRSANGAILAYDITKRSSFLSVPHWIEDVRKYAGSNIVQLLIGNK 132

HSP 2 Score: 39.6614 bits (91), Expect = 7.959e-40
Identity = 22/52 (42.31%), Postives = 34/52 (65.38%), Query Frame = 2

Query:  500 LXNKCDLTANKXVSYETAKAFADEIGIPF-METSAKDSTNVEQAFMAMAASI 652
            + NK DL+  + VS   A++ A+   I   +ETSAKDS+NVE+AF+ +A  +
Sbjct:  129 IGNKSDLSELREVSLAEAQSLAEHYDILCAIETSAKDSSNVEEAFLRVATEL 180

BLAST of CF417146 vs. ExPASy Swiss-Prot
Match: RAB19_RAT (Ras-related protein Rab-19 OS=Rattus norvegicus GN=Rab19 PE=2 SV=1)

HSP 1 Score: 145.976 bits (367), Expect = 1.036e-39
Identity = 65/123 (52.85%), Postives = 92/123 (74.80%), Query Frame = 1

Query:  142 TMNPEYDYLFKLLLIGDSGVGKSCLLLRFADDSYIESYISTIGVDFKIRTVEQDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYASDNVNKLLVGKQ 510
            T++   DYLFK++LIGDS VGK+C++  F    Y ES  +TIGVDF +R++E DGK +K+Q+WDTAGQERFRTIT SYYR AH  II YD+T + +F +V  W++EI++Y + N+  +L+G +
Sbjct:    9 TVDENVDYLFKVILIGDSNVGKTCVVQHFKSGVYSESQQNTIGVDFTVRSLEIDGKKVKMQVWDTAGQERFRTITQSYYRSAHAAIIAYDLTRRSTFESVPHWIHEIEKYGAANLVIMLIGNK 131

HSP 2 Score: 38.5058 bits (88), Expect = 1.036e-39
Identity = 19/52 (36.54%), Postives = 32/52 (61.54%), Query Frame = 2

Query:  500 LXNKCDLTANKXVSYETAKAFADEIGI-PFMETSAKDSTNVEQAFMAMAASI 652
            + NK DL   + V +E A   A++ G+   +ETSAK+S N+++ F+ MA  +
Sbjct:  128 IGNKSDLWEKRHVLFEDACTLAEKYGLLAVLETSAKESRNIDEVFVLMAKEL 179

BLAST of CF417146 vs. ExPASy Swiss-Prot
Match: RAA2A_ARATH (Ras-related protein RABA2a OS=Arabidopsis thaliana GN=RABA2A PE=2 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 1.036e-39
Identity = 67/119 (56.30%), Postives = 89/119 (74.79%), Query Frame = 1

Query:  154 EYDYLFKLLLIGDSGVGKSCLLLRFADDSYIESYISTIGVDFKIRTVEQDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYASDNVNKLLVGKQ 510
            EYDYLFK++LIGDSGVGKS LL RF  + +     STIGV+F  RT++ +G+T+K QIWDTAGQER+R ITS+YYRGA G ++VYDVT   +F NV +WL E+  +A  N+  +L+G +
Sbjct:    8 EYDYLFKVVLIGDSGVGKSNLLSRFTRNEFCLESKSTIGVEFATRTLQVEGRTVKAQIWDTAGQERYRAITSAYYRGALGALLVYDVTKPTTFENVSRWLKELRDHADSNIVIMLIGNK 126

HSP 2 Score: 42.3578 bits (98), Expect = 1.036e-39
Identity = 19/51 (37.25%), Postives = 35/51 (68.63%), Query Frame = 2

Query:  500 LXNKCDLTANKXVSYETAKAFADEIGIPFMETSAKDSTNVEQAFMAMAASI 652
            + NK DL   + V+ E A+++A++ G+ F+ETSA ++ NVE+AF  + + +
Sbjct:  123 IGNKTDLKHLRAVATEDAQSYAEKEGLSFIETSALEALNVEKAFQTILSEV 173

BLAST of CF417146 vs. ExPASy Swiss-Prot
Match: RAB19_MOUSE (Ras-related protein Rab-19 OS=Mus musculus GN=Rab19 PE=2 SV=3)

HSP 1 Score: 146.362 bits (368), Expect = 1.350e-39
Identity = 66/125 (52.80%), Postives = 92/125 (73.60%), Query Frame = 1

Query:  136 STTMNPEYDYLFKLLLIGDSGVGKSCLLLRFADDSYIESYISTIGVDFKIRTVEQDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYASDNVNKLLVGKQ 510
            S T +   DYLFK++LIGDS VGK+C++  F    Y ES  +TIGVDF +R++E DGK +K+Q+WDTAGQERFRTIT SYYR AH  II YD+T + +F +V  W++EI++Y + N+  +L+G +
Sbjct:    7 SRTSDENVDYLFKVILIGDSNVGKTCVVQHFKSGVYSESQQNTIGVDFTVRSLEIDGKKVKMQVWDTAGQERFRTITQSYYRSAHAAIIAYDLTRRSTFESVPHWIHEIEKYGAANLVIMLIGNK 131

HSP 2 Score: 37.7354 bits (86), Expect = 1.350e-39
Identity = 19/52 (36.54%), Postives = 32/52 (61.54%), Query Frame = 2

Query:  500 LXNKCDLTANKXVSYETAKAFADEIGI-PFMETSAKDSTNVEQAFMAMAASI 652
            + NK DL   + V +E A   A++ G+   +ETSAK+S N+++ F+ MA  +
Sbjct:  128 IGNKSDLWEKRHVLFEDACTLAEKHGLLAVLETSAKESRNIDEVFVLMAKEL 179

The following BLAST results are available for this feature:

BLAST of CF417146 vs. ExPASy Swiss-Prot

BLAST of CF417146 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
RAB2A_MOUSE	6.109e-40	52.54	Ras-related protein Rab-2A OS=Mus musculus GN=Rab2...	[more]
RAB2A_MACFA	6.109e-40	52.54	Ras-related protein Rab-2A OS=Macaca fascicularis ...	[more]
RAB2A_HUMAN	6.109e-40	52.54	Ras-related protein Rab-2A OS=Homo sapiens GN=RAB2...	[more]
RAB2A_CHICK	6.109e-40	52.54	Ras-related protein Rab-2A OS=Gallus gallus GN=RAB...	[more]
RAB2A_CANFA	6.109e-40	52.54	Ras-related protein Rab-2A OS=Canis familiaris GN=...	[more]
RAA2C_ARATH	7.955e-40	54.10	Ras-related protein RABA2c OS=Arabidopsis thaliana...	[more]
RAB43_HUMAN	7.959e-40	53.78	Ras-related protein Rab-43 OS=Homo sapiens GN=RAB4...	[more]
RAB19_RAT	1.036e-39	52.85	Ras-related protein Rab-19 OS=Rattus norvegicus GN...	[more]
RAA2A_ARATH	1.036e-39	56.30	Ras-related protein RABA2a OS=Arabidopsis thaliana...	[more]
RAB19_MOUSE	1.350e-39	52.80	Ras-related protein Rab-19 OS=Mus musculus GN=Rab1...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_115000-076 Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange Citrus sinensis cDNA clone IDFL-001_H8 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF417146 ID=CF417146; Name=CF417146; organism=Citrus sinensis; type=EST; length=679bp
TTTTTTTTCTTTTTTGTCTTGTCTTCTGTTTTTGTTGACATTTAATTTCC
CGGCGAAACTCATATCACGAGATCTCGCCGAAGATAATAATCTGTTTTGG
TTAACCGAACCCGAAGCTTTTTTCTTTTACACAAATCTACGACGATGAAT
CCTGAATATGACTATCTGTTTAAGCTTCTGCTTATTGGAGATTCCGGTGT
TGGCAAATCTTGTCTTCTCTTGAGATTTGCTGATGATTCATATATTGAGA
GTTACATCAGCACCATAGGAGTTGATTTTAAAATACGCACTGTTGAGCAA
GATGGGAAGACCATTAAACTTCAAATTTGGGATACTGCTGGTCAAGAAAG
ATTTAGGACAATCACCAGCAGCTACTACCGTGGGGCACATGGTATCATAA
TTGTTTATGATGTGACAGACCAAGAGAGCTTCAATAATGTCAAGCAGTGG
TTGAATGAAATTGACCGTTATGCTAGTGATAATGTCAACAAACTTCTGGT
TGGNAAACAAGTGTGATCTCACTGCAAATAAGGNTGTCTCGTACGAAACA
GCCAAGGCATTTGCAGATGAAATTGGAATACCTTTCATGGAAACTAGTGC
AAAAGATTCTACCAACGTGGAACAGGCATTCATGGCCATGGCTGCTTCAA
TCAAGGATAGAATGGCAAGTCAACCATCC