CF417146

Overview
NameCF417146
Unique NameCF417146
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length679
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CF417146 vs. ExPASy Swiss-Prot
Match: RAS_LACBI (Ras-like protein OS=Laccaria bicolor PE=2 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 3.593e-18
Identity = 32/111 (28.83%), Postives = 59/111 (53.15%), Query Frame = 1 
Query:  169 FKLLLIGDSGVGKSCLLLRFADDSYIESYISTIGVDFKIRTVEQDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYASDNVNKLLV 501
            +KL+++G  G GKSCL ++     +++ Y  TI   ++ + V  D   + L + DTAGQE +  +   Y R   G ++VY +T ++SF  +  +  +I R    +   ++V
Sbjct:    9 YKLVVVGGGGEGKSCLTIQLIQSHFVDEYDPTIEESYRKQCVIDDEVAL-LDVLDTAGQEEYSAMREQYMRTGEGFLLVYSITSRQSFEEIMTFQQQILRVKDKDYFPIIV 118

HSP 2 Score: 45.0542 bits (105), Expect = 3.593e-18
Identity = 24/60 (40.00%), Postives = 34/60 (56.67%), Query Frame = 2 
Query:  506 NKCDLTANKXVSYETAKAFADEIGIPFMETSAKDSTNVEQAFMAMAASIK--DRMASQPS 679
            NKCDL   + VS +  ++ A + G  F+ETSAK   NVE AF  +   I+  ++  S PS
Sbjct:  121 NKCDLDKERVVSKQEGESLARQFGCKFIETSAKSRINVENAFYDLVREIRRYNKEMSNPS 180
[Close]
BLAST of CF417146 vs. ExPASy Swiss-Prot
Match: RASB_DICDI (Ras-like protein rasB OS=Dictyostelium discoideum GN=rasB PE=1 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 3.601e-18
Identity = 42/115 (36.52%), Postives = 63/115 (54.78%), Query Frame = 1 
Query:  169 FKLLLIGDSGVGKSCLLLRFADDSYIESYISTIGVDFKIRTVEQDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYA-SDNVNKLLVGKQ 510
            +KL+++G  GVGKS L ++F  + +IE Y  TI   ++ R  + D  T  L I DTAGQ+ +  +   Y R   G + VYDVT + SF  +     +I R   +D V  +LVG +
Sbjct:    7 YKLVVMGGGGVGKSALTIQFIQNHFIEEYDPTIEDSYR-RQCQVDEDTCLLDILDTAGQDDYSAMRDQYMRTGQGFLCVYDVTSRTSFEEINVVREQIIRVKDNDKVPIVLVGNK 120

HSP 2 Score: 40.4318 bits (93), Expect = 3.601e-18
Identity = 19/50 (38.00%), Postives = 26/50 (52.00%), Query Frame = 2 
Query:  506 NKCDLTANKXVSYETAKAFADEIGIPFMETSAKDSTNVEQAFMAMAASIK 655
            NKCDL   + V+       A    +PF+ETSAK   NV++ F  +   IK
Sbjct:  119 NKCDLENLREVTEGEGSELAKSFSVPFLETSAKKRLNVDECFFEVVREIK 168
[Close]
BLAST of CF417146 vs. ExPASy Swiss-Prot
Match: RASN_XENLA (GTPase NRas OS=Xenopus laevis GN=nras PE=2 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 3.608e-18
Identity = 38/115 (33.04%), Postives = 66/115 (57.39%), Query Frame = 1 
Query:  169 FKLLLIGDSGVGKSCLLLRFADDSYIESYISTIGVDFKIRTVEQDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYA-SDNVNKLLVGKQ 510
            +KL+++G  GVGKS L ++   + +++ Y  TI   ++ + V  DG+T  L I DTAGQE +  +   Y R   G + V+ + + +SF ++  +  +I R   SD+V  +LVG +
Sbjct:    4 YKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYR-KQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNSKSFADINAYREQIKRVKDSDDVPMVLVGNK 117

HSP 2 Score: 40.4318 bits (93), Expect = 3.608e-18
Identity = 22/49 (44.90%), Postives = 28/49 (57.14%), Query Frame = 2 
Query:  506 NKCDLTANKXVSYETAKAFADEIGIPFMETSAKDSTNVEQAFMAMAASI 652
            NKCDL + + V  + A+  A   GIPF+ETSAK    VE AF  +   I
Sbjct:  116 NKCDLPS-RTVDTKQAQELARSYGIPFIETSAKTRQGVEDAFYTLVREI 163
[Close]
BLAST of CF417146 vs. ExPASy Swiss-Prot
Match: RAB6C_HUMAN (Ras-related protein Rab-6C OS=Homo sapiens GN=RAB6C PE=2 SV=2)

HSP 1 Score: 91.6633 bits (226), Expect = 3.938e-18
Identity = 42/114 (36.84%), Postives = 70/114 (61.40%), Query Frame = 1 
Query:  169 FKLLLIGDSGVGKSCLLLRFADDSYIESYISTIGVDFKIRTVEQDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYASDNVNKLLVGKQ 510
            FKL+ +G+  V K+ L+ RF  DS+  +Y + IG+DF  +T+  +  TI L++WDTAGQER R++   Y R +   ++VYD+T+  SF    +W++++      +V   LVG +
Sbjct:   14 FKLVFLGEQSVAKTSLITRFRYDSFDNTYQAIIGIDFLSKTMYLEDGTIGLRLWDTAGQERLRSLIPRYIRDSAAAVVVYDITNVNSFQQTTKWIDDVRTERGSDVIITLVGNR 127
[Close]
BLAST of CF417146 vs. ExPASy Swiss-Prot
Match: RASN_RAT (GTPase NRas OS=Rattus norvegicus GN=Nras PE=2 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 4.692e-18
Identity = 38/115 (33.04%), Postives = 66/115 (57.39%), Query Frame = 1 
Query:  169 FKLLLIGDSGVGKSCLLLRFADDSYIESYISTIGVDFKIRTVEQDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYA-SDNVNKLLVGKQ 510
            +KL+++G  GVGKS L ++   + +++ Y  TI   ++ + V  DG+T  L I DTAGQE +  +   Y R   G + V+ + + +SF ++  +  +I R   SD+V  +LVG +
Sbjct:    4 YKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYR-KQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNSKSFADINLYREQIKRVKDSDDVPMVLVGNK 117

HSP 2 Score: 40.4318 bits (93), Expect = 4.692e-18
Identity = 22/50 (44.00%), Postives = 27/50 (54.00%), Query Frame = 2 
Query:  506 NKCDLTANKXVSYETAKAFADEIGIPFMETSAKDSTNVEQAFMAMAASIK 655
            NKCDL   + V  + A   A   GIPF+ETSAK    VE AF  +   I+
Sbjct:  116 NKCDLPT-RTVDTKQAHELAKSYGIPFIETSAKTRQGVEDAFYTLVREIR 164
[Close]
BLAST of CF417146 vs. ExPASy Swiss-Prot
Match: RASN_PIG (GTPase NRas OS=Sus scrofa GN=NRAS PE=2 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 4.692e-18
Identity = 38/115 (33.04%), Postives = 66/115 (57.39%), Query Frame = 1 
Query:  169 FKLLLIGDSGVGKSCLLLRFADDSYIESYISTIGVDFKIRTVEQDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYA-SDNVNKLLVGKQ 510
            +KL+++G  GVGKS L ++   + +++ Y  TI   ++ + V  DG+T  L I DTAGQE +  +   Y R   G + V+ + + +SF ++  +  +I R   SD+V  +LVG +
Sbjct:    4 YKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYR-KQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNSKSFADINLYREQIKRVKDSDDVPMVLVGNK 117

HSP 2 Score: 40.4318 bits (93), Expect = 4.692e-18
Identity = 22/50 (44.00%), Postives = 27/50 (54.00%), Query Frame = 2 
Query:  506 NKCDLTANKXVSYETAKAFADEIGIPFMETSAKDSTNVEQAFMAMAASIK 655
            NKCDL   + V  + A   A   GIPF+ETSAK    VE AF  +   I+
Sbjct:  116 NKCDLPT-RTVDTKQAHELAKSYGIPFIETSAKTRQGVEDAFYTLVREIR 164
[Close]
BLAST of CF417146 vs. ExPASy Swiss-Prot
Match: RASN_MOUSE (GTPase NRas OS=Mus musculus GN=Nras PE=2 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 4.692e-18
Identity = 38/115 (33.04%), Postives = 66/115 (57.39%), Query Frame = 1 
Query:  169 FKLLLIGDSGVGKSCLLLRFADDSYIESYISTIGVDFKIRTVEQDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYA-SDNVNKLLVGKQ 510
            +KL+++G  GVGKS L ++   + +++ Y  TI   ++ + V  DG+T  L I DTAGQE +  +   Y R   G + V+ + + +SF ++  +  +I R   SD+V  +LVG +
Sbjct:    4 YKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYR-KQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNSKSFADINLYREQIKRVKDSDDVPMVLVGNK 117

HSP 2 Score: 40.4318 bits (93), Expect = 4.692e-18
Identity = 22/50 (44.00%), Postives = 27/50 (54.00%), Query Frame = 2 
Query:  506 NKCDLTANKXVSYETAKAFADEIGIPFMETSAKDSTNVEQAFMAMAASIK 655
            NKCDL   + V  + A   A   GIPF+ETSAK    VE AF  +   I+
Sbjct:  116 NKCDLPT-RTVDTKQAHELAKSYGIPFIETSAKTRQGVEDAFYTLVREIR 164
[Close]
BLAST of CF417146 vs. ExPASy Swiss-Prot
Match: RASN_MONDO (GTPase NRas OS=Monodelphis domestica GN=NRAS PE=2 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 4.692e-18
Identity = 38/115 (33.04%), Postives = 66/115 (57.39%), Query Frame = 1 
Query:  169 FKLLLIGDSGVGKSCLLLRFADDSYIESYISTIGVDFKIRTVEQDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYA-SDNVNKLLVGKQ 510
            +KL+++G  GVGKS L ++   + +++ Y  TI   ++ + V  DG+T  L I DTAGQE +  +   Y R   G + V+ + + +SF ++  +  +I R   SD+V  +LVG +
Sbjct:    4 YKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYR-KQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNSKSFADINLYREQIKRVKDSDDVPMVLVGNK 117

HSP 2 Score: 40.4318 bits (93), Expect = 4.692e-18
Identity = 22/50 (44.00%), Postives = 27/50 (54.00%), Query Frame = 2 
Query:  506 NKCDLTANKXVSYETAKAFADEIGIPFMETSAKDSTNVEQAFMAMAASIK 655
            NKCDL   + V  + A   A   GIPF+ETSAK    VE AF  +   I+
Sbjct:  116 NKCDLPT-RTVDTKQAHELAKSYGIPFIETSAKTRQGVEDAFYTLVREIR 164
[Close]
BLAST of CF417146 vs. ExPASy Swiss-Prot
Match: RASN_HUMAN (GTPase NRas OS=Homo sapiens GN=NRAS PE=1 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 4.692e-18
Identity = 38/115 (33.04%), Postives = 66/115 (57.39%), Query Frame = 1 
Query:  169 FKLLLIGDSGVGKSCLLLRFADDSYIESYISTIGVDFKIRTVEQDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYA-SDNVNKLLVGKQ 510
            +KL+++G  GVGKS L ++   + +++ Y  TI   ++ + V  DG+T  L I DTAGQE +  +   Y R   G + V+ + + +SF ++  +  +I R   SD+V  +LVG +
Sbjct:    4 YKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYR-KQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNSKSFADINLYREQIKRVKDSDDVPMVLVGNK 117

HSP 2 Score: 40.4318 bits (93), Expect = 4.692e-18
Identity = 22/50 (44.00%), Postives = 27/50 (54.00%), Query Frame = 2 
Query:  506 NKCDLTANKXVSYETAKAFADEIGIPFMETSAKDSTNVEQAFMAMAASIK 655
            NKCDL   + V  + A   A   GIPF+ETSAK    VE AF  +   I+
Sbjct:  116 NKCDLPT-RTVDTKQAHELAKSYGIPFIETSAKTRQGVEDAFYTLVREIR 164
[Close]
BLAST of CF417146 vs. ExPASy Swiss-Prot
Match: RASN_CAVPO (GTPase NRas OS=Cavia porcellus GN=NRAS PE=2 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 4.692e-18
Identity = 38/115 (33.04%), Postives = 66/115 (57.39%), Query Frame = 1 
Query:  169 FKLLLIGDSGVGKSCLLLRFADDSYIESYISTIGVDFKIRTVEQDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYA-SDNVNKLLVGKQ 510
            +KL+++G  GVGKS L ++   + +++ Y  TI   ++ + V  DG+T  L I DTAGQE +  +   Y R   G + V+ + + +SF ++  +  +I R   SD+V  +LVG +
Sbjct:    4 YKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYR-KQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNSKSFADINLYREQIKRVKDSDDVPMVLVGNK 117

HSP 2 Score: 40.4318 bits (93), Expect = 4.692e-18
Identity = 22/50 (44.00%), Postives = 27/50 (54.00%), Query Frame = 2 
Query:  506 NKCDLTANKXVSYETAKAFADEIGIPFMETSAKDSTNVEQAFMAMAASIK 655
            NKCDL   + V  + A   A   GIPF+ETSAK    VE AF  +   I+
Sbjct:  116 NKCDLPT-RTVDTKQAHELAKSYGIPFIETSAKTRQGVEDAFYTLVREIR 164
The following BLAST results are available for this feature:
BLAST of CF417146 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
RAS_LACBI3.593e-1828.83Ras-like protein OS=Laccaria bicolor PE=2 SV=1[more]
RASB_DICDI3.601e-1836.52Ras-like protein rasB OS=Dictyostelium discoideum ... [more]
RASN_XENLA3.608e-1833.04GTPase NRas OS=Xenopus laevis GN=nras PE=2 SV=1[more]
RAB6C_HUMAN3.938e-1836.84Ras-related protein Rab-6C OS=Homo sapiens GN=RAB6... [more]
RASN_RAT4.692e-1833.04GTPase NRas OS=Rattus norvegicus GN=Nras PE=2 SV=1[more]
RASN_PIG4.692e-1833.04GTPase NRas OS=Sus scrofa GN=NRAS PE=2 SV=1[more]
RASN_MOUSE4.692e-1833.04GTPase NRas OS=Mus musculus GN=Nras PE=2 SV=1[more]
RASN_MONDO4.692e-1833.04GTPase NRas OS=Monodelphis domestica GN=NRAS PE=2 ... [more]
RASN_HUMAN4.692e-1833.04GTPase NRas OS=Homo sapiens GN=NRAS PE=1 SV=1[more]
RASN_CAVPO4.692e-1833.04GTPase NRas OS=Cavia porcellus GN=NRAS PE=2 SV=1[more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_115000-076 Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange Citrus sinensis cDNA clone IDFL-001_H8 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF417146 ID=CF417146; Name=CF417146; organism=Citrus sinensis; type=EST; length=679bp
TTTTTTTTCTTTTTTGTCTTGTCTTCTGTTTTTGTTGACATTTAATTTCC
CGGCGAAACTCATATCACGAGATCTCGCCGAAGATAATAATCTGTTTTGG
TTAACCGAACCCGAAGCTTTTTTCTTTTACACAAATCTACGACGATGAAT
CCTGAATATGACTATCTGTTTAAGCTTCTGCTTATTGGAGATTCCGGTGT
TGGCAAATCTTGTCTTCTCTTGAGATTTGCTGATGATTCATATATTGAGA
GTTACATCAGCACCATAGGAGTTGATTTTAAAATACGCACTGTTGAGCAA
GATGGGAAGACCATTAAACTTCAAATTTGGGATACTGCTGGTCAAGAAAG
ATTTAGGACAATCACCAGCAGCTACTACCGTGGGGCACATGGTATCATAA
TTGTTTATGATGTGACAGACCAAGAGAGCTTCAATAATGTCAAGCAGTGG
TTGAATGAAATTGACCGTTATGCTAGTGATAATGTCAACAAACTTCTGGT
TGGNAAACAAGTGTGATCTCACTGCAAATAAGGNTGTCTCGTACGAAACA
GCCAAGGCATTTGCAGATGAAATTGGAATACCTTTCATGGAAACTAGTGC
AAAAGATTCTACCAACGTGGAACAGGCATTCATGGCCATGGCTGCTTCAA
TCAAGGATAGAATGGCAAGTCAACCATCC
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