CF417146 | Citrus Genome Database

Overview


Name	CF417146
Unique Name	CF417146
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	679

Libraries


Library Name	Type
Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

BLAST of CF417146 vs. ExPASy Swiss-Prot
Match: RAB3A_PIG (Ras-related protein Rab-3A OS=Sus scrofa GN=RAB3A PE=2 SV=1)

HSP 1 Score: 154.451 bits (389), Expect = 5.332e-45
Identity = 66/118 (55.93%), Postives = 96/118 (81.36%), Query Frame = 1

Query:  157 YDYLFKLLLIGDSGVGKSCLLLRFADDSYIESYISTIGVDFKIRTVEQDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYASDNVNKLLVGKQ 510
            +DY+FK+L+IG+S VGK+  L R+ADDS+  +++ST+G+DFK++T+ ++ K IKLQIWDTAGQER+RTIT++YYRGA G I++YD+T++ESFN V+ W  +I  Y+ DN   LLVG +
Sbjct:   19 FDYMFKILIIGNSSVGKTSFLFRYADDSFTPAFVSTVGIDFKVKTIYRNDKRIKLQIWDTAGQERYRTITTAYYRGAMGFILMYDITNEESFNAVQDWSTQIKTYSWDNAQVLLVGNK 136

HSP 2 Score: 47.7506 bits (112), Expect = 5.332e-45
Identity = 22/54 (40.74%), Postives = 32/54 (59.26%), Query Frame = 2

Query:  506 NKCDLTANKXVSYETAKAFADEIGIPFMETSAKDSTNVEQAFMAMAASIKDRMA 667
            NKCD+   + VS E  +  AD +G  F E SAKD+ NV+Q F  +   I ++M+
Sbjct:  135 NKCDMEDERVVSSERGRQLADHLGFEFFEASAKDNINVKQTFERLVDVICEKMS 188

BLAST of CF417146 vs. ExPASy Swiss-Prot
Match: RAB3A_MOUSE (Ras-related protein Rab-3A OS=Mus musculus GN=Rab3a PE=1 SV=1)

HSP 1 Score: 154.451 bits (389), Expect = 5.332e-45
Identity = 66/118 (55.93%), Postives = 96/118 (81.36%), Query Frame = 1

Query:  157 YDYLFKLLLIGDSGVGKSCLLLRFADDSYIESYISTIGVDFKIRTVEQDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYASDNVNKLLVGKQ 510
            +DY+FK+L+IG+S VGK+  L R+ADDS+  +++ST+G+DFK++T+ ++ K IKLQIWDTAGQER+RTIT++YYRGA G I++YD+T++ESFN V+ W  +I  Y+ DN   LLVG +
Sbjct:   19 FDYMFKILIIGNSSVGKTSFLFRYADDSFTPAFVSTVGIDFKVKTIYRNDKRIKLQIWDTAGQERYRTITTAYYRGAMGFILMYDITNEESFNAVQDWSTQIKTYSWDNAQVLLVGNK 136

HSP 2 Score: 47.7506 bits (112), Expect = 5.332e-45
Identity = 22/54 (40.74%), Postives = 32/54 (59.26%), Query Frame = 2

Query:  506 NKCDLTANKXVSYETAKAFADEIGIPFMETSAKDSTNVEQAFMAMAASIKDRMA 667
            NKCD+   + VS E  +  AD +G  F E SAKD+ NV+Q F  +   I ++M+
Sbjct:  135 NKCDMEDERVVSSERGRQLADHLGFEFFEASAKDNINVKQTFERLVDVICEKMS 188

BLAST of CF417146 vs. ExPASy Swiss-Prot
Match: RAB3A_MACFA (Ras-related protein Rab-3A OS=Macaca fascicularis GN=RAB3A PE=2 SV=1)

HSP 1 Score: 154.451 bits (389), Expect = 5.332e-45
Identity = 66/118 (55.93%), Postives = 96/118 (81.36%), Query Frame = 1

Query:  157 YDYLFKLLLIGDSGVGKSCLLLRFADDSYIESYISTIGVDFKIRTVEQDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYASDNVNKLLVGKQ 510
            +DY+FK+L+IG+S VGK+  L R+ADDS+  +++ST+G+DFK++T+ ++ K IKLQIWDTAGQER+RTIT++YYRGA G I++YD+T++ESFN V+ W  +I  Y+ DN   LLVG +
Sbjct:   19 FDYMFKILIIGNSSVGKTSFLFRYADDSFTPAFVSTVGIDFKVKTIYRNDKRIKLQIWDTAGQERYRTITTAYYRGAMGFILMYDITNEESFNAVQDWSTQIKTYSWDNAQVLLVGNK 136

HSP 2 Score: 47.7506 bits (112), Expect = 5.332e-45
Identity = 22/54 (40.74%), Postives = 32/54 (59.26%), Query Frame = 2

Query:  506 NKCDLTANKXVSYETAKAFADEIGIPFMETSAKDSTNVEQAFMAMAASIKDRMA 667
            NKCD+   + VS E  +  AD +G  F E SAKD+ NV+Q F  +   I ++M+
Sbjct:  135 NKCDMEDERVVSSERGRQLADHLGFEFFEASAKDNINVKQTFERLVDVICEKMS 188

BLAST of CF417146 vs. ExPASy Swiss-Prot
Match: RAB3A_HUMAN (Ras-related protein Rab-3A OS=Homo sapiens GN=RAB3A PE=1 SV=1)

HSP 1 Score: 154.451 bits (389), Expect = 5.332e-45
Identity = 66/118 (55.93%), Postives = 96/118 (81.36%), Query Frame = 1

Query:  157 YDYLFKLLLIGDSGVGKSCLLLRFADDSYIESYISTIGVDFKIRTVEQDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYASDNVNKLLVGKQ 510
            +DY+FK+L+IG+S VGK+  L R+ADDS+  +++ST+G+DFK++T+ ++ K IKLQIWDTAGQER+RTIT++YYRGA G I++YD+T++ESFN V+ W  +I  Y+ DN   LLVG +
Sbjct:   19 FDYMFKILIIGNSSVGKTSFLFRYADDSFTPAFVSTVGIDFKVKTIYRNDKRIKLQIWDTAGQERYRTITTAYYRGAMGFILMYDITNEESFNAVQDWSTQIKTYSWDNAQVLLVGNK 136

HSP 2 Score: 47.7506 bits (112), Expect = 5.332e-45
Identity = 22/54 (40.74%), Postives = 32/54 (59.26%), Query Frame = 2

Query:  506 NKCDLTANKXVSYETAKAFADEIGIPFMETSAKDSTNVEQAFMAMAASIKDRMA 667
            NKCD+   + VS E  +  AD +G  F E SAKD+ NV+Q F  +   I ++M+
Sbjct:  135 NKCDMEDERVVSSERGRQLADHLGFEFFEASAKDNINVKQTFERLVDVICEKMS 188

BLAST of CF417146 vs. ExPASy Swiss-Prot
Match: RAB3A_BOVIN (Ras-related protein Rab-3A OS=Bos taurus GN=RAB3A PE=1 SV=3)

HSP 1 Score: 154.451 bits (389), Expect = 5.332e-45
Identity = 66/118 (55.93%), Postives = 96/118 (81.36%), Query Frame = 1

Query:  157 YDYLFKLLLIGDSGVGKSCLLLRFADDSYIESYISTIGVDFKIRTVEQDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYASDNVNKLLVGKQ 510
            +DY+FK+L+IG+S VGK+  L R+ADDS+  +++ST+G+DFK++T+ ++ K IKLQIWDTAGQER+RTIT++YYRGA G I++YD+T++ESFN V+ W  +I  Y+ DN   LLVG +
Sbjct:   19 FDYMFKILIIGNSSVGKTSFLFRYADDSFTPAFVSTVGIDFKVKTIYRNDKRIKLQIWDTAGQERYRTITTAYYRGAMGFILMYDITNEESFNAVQDWSTQIKTYSWDNAQVLLVGNK 136

HSP 2 Score: 47.7506 bits (112), Expect = 5.332e-45
Identity = 22/54 (40.74%), Postives = 32/54 (59.26%), Query Frame = 2

Query:  506 NKCDLTANKXVSYETAKAFADEIGIPFMETSAKDSTNVEQAFMAMAASIKDRMA 667
            NKCD+   + VS E  +  AD +G  F E SAKD+ NV+Q F  +   I ++M+
Sbjct:  135 NKCDMEDERVVSSERGRQLADHLGFEFFEASAKDNINVKQTFERLVDVICEKMS 188

BLAST of CF417146 vs. ExPASy Swiss-Prot
Match: RAB3C_RAT (Ras-related protein Rab-3C OS=Rattus norvegicus GN=Rab3c PE=1 SV=1)

HSP 1 Score: 152.91 bits (385), Expect = 9.052e-45
Identity = 66/118 (55.93%), Postives = 95/118 (80.51%), Query Frame = 1

Query:  157 YDYLFKLLLIGDSGVGKSCLLLRFADDSYIESYISTIGVDFKIRTVEQDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYASDNVNKLLVGKQ 510
            +DY+FKLL+IG+S VGK+  L R+ADDS+  +++ST+G+DFK++TV ++ K IKLQIWDTAGQER+RTIT++YYRGA G I++YD+T++ESFN V+ W  +I  Y+ DN   +L G +
Sbjct:   27 FDYMFKLLIIGNSSVGKTSFLFRYADDSFTSAFVSTVGIDFKVKTVFKNEKRIKLQIWDTAGQERYRTITTAYYRGAMGFILMYDITNEESFNAVQDWSTQIKTYSWDNAQVILAGNK 144

HSP 2 Score: 48.521 bits (114), Expect = 9.052e-45
Identity = 22/54 (40.74%), Postives = 33/54 (61.11%), Query Frame = 2

Query:  506 NKCDLTANKXVSYETAKAFADEIGIPFMETSAKDSTNVEQAFMAMAASIKDRMA 667
            NKCD+   + VS E  +   +++G  F ETSAKD+ NV+Q F  +   I D+M+
Sbjct:  143 NKCDMEDERVVSTERGQRLGEQLGFEFFETSAKDNINVKQTFERLVDIICDKMS 196

BLAST of CF417146 vs. ExPASy Swiss-Prot
Match: RAB3C_MOUSE (Ras-related protein Rab-3C OS=Mus musculus GN=Rab3c PE=1 SV=1)

HSP 1 Score: 152.91 bits (385), Expect = 9.052e-45
Identity = 66/118 (55.93%), Postives = 95/118 (80.51%), Query Frame = 1

Query:  157 YDYLFKLLLIGDSGVGKSCLLLRFADDSYIESYISTIGVDFKIRTVEQDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYASDNVNKLLVGKQ 510
            +DY+FKLL+IG+S VGK+  L R+ADDS+  +++ST+G+DFK++TV ++ K IKLQIWDTAGQER+RTIT++YYRGA G I++YD+T++ESFN V+ W  +I  Y+ DN   +L G +
Sbjct:   27 FDYMFKLLIIGNSSVGKTSFLFRYADDSFTSAFVSTVGIDFKVKTVFKNEKRIKLQIWDTAGQERYRTITTAYYRGAMGFILMYDITNEESFNAVQDWSTQIKTYSWDNAQVILAGNK 144

HSP 2 Score: 48.521 bits (114), Expect = 9.052e-45
Identity = 22/54 (40.74%), Postives = 33/54 (61.11%), Query Frame = 2

Query:  506 NKCDLTANKXVSYETAKAFADEIGIPFMETSAKDSTNVEQAFMAMAASIKDRMA 667
            NKCD+   + VS E  +   +++G  F ETSAKD+ NV+Q F  +   I D+M+
Sbjct:  143 NKCDMEDERVVSTERGQRLGEQLGFEFFETSAKDNINVKQTFERLVDIICDKMS 196

BLAST of CF417146 vs. ExPASy Swiss-Prot
Match: RAB26_BOVIN (Ras-related protein Rab-26 OS=Bos taurus GN=RAB26 PE=2 SV=1)

HSP 1 Score: 146.747 bits (369), Expect = 3.396e-44
Identity = 63/120 (52.50%), Postives = 97/120 (80.83%), Query Frame = 1

Query:  157 YDYLFKLLLIGDSGVGKSCLLLRFADDSYIE-SYISTIGVDFKIRTVEQDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYASDNVNKLLVGKQV 513
            YD  FK++L+GDSGVGK+CLL+RF D +++  ++IST+G+DF+ + V+ DG  +KLQIWDTAGQERFR++T +YYR AH ++++YDVT++ SF++++ WL EI  +A D+V  +L+G +V
Sbjct:   60 YDVAFKVMLVGDSGVGKTCLLVRFKDGAFLAGTFISTVGIDFRNKVVDVDGMKVKLQIWDTAGQERFRSVTHAYYRDAHALLLLYDVTNKASFDSIQAWLTEIQEHAQDDVVLMLLGNKV 179

HSP 2 Score: 52.7582 bits (125), Expect = 3.396e-44
Identity = 27/60 (45.00%), Postives = 34/60 (56.67%), Query Frame = 2

Query:  500 LXNKCDLTANKXVSYETAKAFADEIGIPFMETSAKDSTNVEQAFMAMAASIKDRMASQPS 679
            L NK D    + V  E A+  A + G+PFMETSAK   NV+ AF A+A  +K R    PS
Sbjct:  175 LGNKVDSAQERAVKREDAEKLAKDYGLPFMETSAKTGLNVDLAFTAIAKELKQRHTKAPS 234

BLAST of CF417146 vs. ExPASy Swiss-Prot
Match: RABF1_DICDI (Ras-related protein RabF1 OS=Dictyostelium discoideum GN=rabF1-1 PE=3 SV=1)

HSP 1 Score: 153.295 bits (386), Expect = 3.413e-44
Identity = 71/122 (58.20%), Postives = 99/122 (81.15%), Query Frame = 1

Query:  145 MNPEYDYLFKLLLIGDSGVGKSCLLLRFADDSYIESYISTIGVDFKIRTVEQDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYASDNVNKLLVGKQ 510
            M+ EY++LFK +L GDSGVGK+ +L R  DD++ E++I TIG++FKI+TV  +GK IKLQIWDTAG++RFR + +S+YRG HG+IIVYDVTDQ SF NV  W+ +I RYA++NV K+++G +
Sbjct:    1 MSKEYEHLFKFVLAGDSGVGKTSILFRITDDTFTETHI-TIGIEFKIKTVYIEGKPIKLQIWDTAGEKRFR-VHNSHYRGCHGVIIVYDVTDQRSFENVPSWIEDIRRYANENVIKIIIGNK 120

HSP 2 Score: 46.2098 bits (108), Expect = 3.413e-44
Identity = 22/47 (46.81%), Postives = 31/47 (65.96%), Query Frame = 2

Query:  500 LXNKCDLTANKXVSYETAKAFADEIGIPFMETSAKDSTNVEQAFMAM 640
            + NK DL + K V    A+ FAD + IPF E SAK S N+E+AF+++
Sbjct:  117 IGNKNDLVSQKVVDPFLAQEFADSLDIPFKEISAKQSINIEEAFISL 163

BLAST of CF417146 vs. ExPASy Swiss-Prot
Match: RAB3B_MOUSE (Ras-related protein Rab-3B OS=Mus musculus GN=Rab3b PE=1 SV=1)

HSP 1 Score: 152.525 bits (384), Expect = 7.535e-44
Identity = 65/118 (55.08%), Postives = 95/118 (80.51%), Query Frame = 1

Query:  157 YDYLFKLLLIGDSGVGKSCLLLRFADDSYIESYISTIGVDFKIRTVEQDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYASDNVNKLLVGKQ 510
            +DY+FKLL+IG+S VGK+  L R+ADD++  +++ST+G+DFK++TV +  K +KLQIWDTAGQER+RTIT++YYRGA G I++YD+T++ESFN V+ W  +I  Y+ DN   +LVG +
Sbjct:   19 FDYMFKLLIIGNSSVGKTSFLFRYADDTFTPAFVSTVGIDFKVKTVYRHEKRVKLQIWDTAGQERYRTITTAYYRGAMGFILMYDITNEESFNAVQDWATQIKTYSWDNAQVILVGNK 136

HSP 2 Score: 45.8246 bits (107), Expect = 7.535e-44
Identity = 20/54 (37.04%), Postives = 33/54 (61.11%), Query Frame = 2

Query:  506 NKCDLTANKXVSYETAKAFADEIGIPFMETSAKDSTNVEQAFMAMAASIKDRMA 667
            NKCD+   + V  E  +  A+++G  F E SAK++ +V QAF  +  +I D+M+
Sbjct:  135 NKCDMEEERVVPTEKGRLLAEQLGFDFFEASAKENISVRQAFERLVDAICDKMS 188

The following BLAST results are available for this feature:

BLAST of CF417146 vs. ExPASy Swiss-Prot

BLAST of CF417146 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
RAB3A_PIG	5.332e-45	55.93	Ras-related protein Rab-3A OS=Sus scrofa GN=RAB3A ...	[more]
RAB3A_MOUSE	5.332e-45	55.93	Ras-related protein Rab-3A OS=Mus musculus GN=Rab3...	[more]
RAB3A_MACFA	5.332e-45	55.93	Ras-related protein Rab-3A OS=Macaca fascicularis ...	[more]
RAB3A_HUMAN	5.332e-45	55.93	Ras-related protein Rab-3A OS=Homo sapiens GN=RAB3...	[more]
RAB3A_BOVIN	5.332e-45	55.93	Ras-related protein Rab-3A OS=Bos taurus GN=RAB3A ...	[more]
RAB3C_RAT	9.052e-45	55.93	Ras-related protein Rab-3C OS=Rattus norvegicus GN...	[more]
RAB3C_MOUSE	9.052e-45	55.93	Ras-related protein Rab-3C OS=Mus musculus GN=Rab3...	[more]
RAB26_BOVIN	3.396e-44	52.50	Ras-related protein Rab-26 OS=Bos taurus GN=RAB26 ...	[more]
RABF1_DICDI	3.413e-44	58.20	Ras-related protein RabF1 OS=Dictyostelium discoid...	[more]
RAB3B_MOUSE	7.535e-44	55.08	Ras-related protein Rab-3B OS=Mus musculus GN=Rab3...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_115000-076 Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange Citrus sinensis cDNA clone IDFL-001_H8 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF417146 ID=CF417146; Name=CF417146; organism=Citrus sinensis; type=EST; length=679bp
TTTTTTTTCTTTTTTGTCTTGTCTTCTGTTTTTGTTGACATTTAATTTCC
CGGCGAAACTCATATCACGAGATCTCGCCGAAGATAATAATCTGTTTTGG
TTAACCGAACCCGAAGCTTTTTTCTTTTACACAAATCTACGACGATGAAT
CCTGAATATGACTATCTGTTTAAGCTTCTGCTTATTGGAGATTCCGGTGT
TGGCAAATCTTGTCTTCTCTTGAGATTTGCTGATGATTCATATATTGAGA
GTTACATCAGCACCATAGGAGTTGATTTTAAAATACGCACTGTTGAGCAA
GATGGGAAGACCATTAAACTTCAAATTTGGGATACTGCTGGTCAAGAAAG
ATTTAGGACAATCACCAGCAGCTACTACCGTGGGGCACATGGTATCATAA
TTGTTTATGATGTGACAGACCAAGAGAGCTTCAATAATGTCAAGCAGTGG
TTGAATGAAATTGACCGTTATGCTAGTGATAATGTCAACAAACTTCTGGT
TGGNAAACAAGTGTGATCTCACTGCAAATAAGGNTGTCTCGTACGAAACA
GCCAAGGCATTTGCAGATGAAATTGGAATACCTTTCATGGAAACTAGTGC
AAAAGATTCTACCAACGTGGAACAGGCATTCATGGCCATGGCTGCTTCAA
TCAAGGATAGAATGGCAAGTCAACCATCC