CF417147 | Citrus Genome Database

Overview


Name	CF417147
Unique Name	CF417147
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	694

Libraries


Library Name	Type
Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CF417147 vs. ExPASy Swiss-Prot
Match: UBIQ_LEIMA (Ubiquitin OS=Leishmania major GN=UB-EP52 PE=3 SV=1)

HSP 1 Score: 140.584 bits (353), Expect = 7.720e-33
Identity = 70/75 (93.33%), Postives = 73/75 (97.33%), Query Frame = 1

Query:    1 QIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 225
            QIFVKTLTGKTI LEVE SDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLE+GRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    2 QIFVKTLTGKTIALEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEEGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 2 Score: 78.9518 bits (193), Expect = 6.432e-22
Identity = 38/44 (86.36%), Postives = 41/44 (93.18%), Query Frame = 2

Query:  257 KQLLLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGR 388
            K + LEVE SDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLE+GR
Sbjct:   11 KTIALEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEEGR 54

HSP 3 Score: 45.8246 bits (107), Expect = 6.432e-22
Identity = 21/22 (95.45%), Postives = 22/22 (100.00%), Query Frame = 3

Query:  390 TLADYNIQKESTLHLVLRLRGG 455
            TL+DYNIQKESTLHLVLRLRGG
Sbjct:   55 TLSDYNIQKESTLHLVLRLRGG 76

HSP 4 Score: 76.6406 bits (187), Expect = 1.369e-13
Identity = 44/65 (67.69%), Postives = 48/65 (73.85%), Query Frame = 1

Query:  484 GKPSTLEVESSDTIDNVKAKIQX*XRGIPPDQPEG*SLLENNLEDGRTLADYNIQKESTLHLVLR 678
            GK   LEVE SDTI+NVKAKIQ    GIPPDQ +        LE+GRTL+DYNIQKESTLHLVLR
Sbjct:   10 GKTIALEVEPSDTIENVKAKIQD-KEGIPPDQ-QRLIFAGKQLEEGRTLSDYNIQKESTLHLVLR 72

[Close]

BLAST of CF417147 vs. ExPASy Swiss-Prot
Match: UBIQ_LEITA (Ubiquitin OS=Leishmania tarentolae GN=UB-EP52 PE=3 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 2.483e-31
Identity = 68/75 (90.67%), Postives = 71/75 (94.67%), Query Frame = 1

Query:    1 QIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 225
            QIFVKTLTG TI LEVE SDTI+NVKAKIQDKEGIPPDQQRLIFA KQLE+GRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    2 QIFVKTLTGTTIALEVEPSDTIENVKAKIQDKEGIPPDQQRLIFADKQLEEGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 2 Score: 74.7146 bits (182), Expect = 8.951e-21
Identity = 36/42 (85.71%), Postives = 39/42 (92.86%), Query Frame = 2

Query:  263 LLLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGR 388
            + LEVE SDTI+NVKAKIQDKEGIPPDQQRLIFA KQLE+GR
Sbjct:   13 IALEVEPSDTIENVKAKIQDKEGIPPDQQRLIFADKQLEEGR 54

HSP 3 Score: 46.2098 bits (108), Expect = 8.951e-21
Identity = 22/30 (73.33%), Postives = 25/30 (83.33%), Query Frame = 3

Query:  366 ESSLKMEGTLADYNIQKESTLHLVLRLRGG 455
            +  L+   TL+DYNIQKESTLHLVLRLRGG
Sbjct:   47 DKQLEEGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 4 Score: 75.8702 bits (185), Expect = 2.335e-13
Identity = 43/65 (66.15%), Postives = 48/65 (73.85%), Query Frame = 1

Query:  484 GKPSTLEVESSDTIDNVKAKIQX*XRGIPPDQPEG*SLLENNLEDGRTLADYNIQKESTLHLVLR 678
            G    LEVE SDTI+NVKAKIQ    GIPPDQ +     +  LE+GRTL+DYNIQKESTLHLVLR
Sbjct:   10 GTTIALEVEPSDTIENVKAKIQD-KEGIPPDQ-QRLIFADKQLEEGRTLSDYNIQKESTLHLVLR 72

[Close]

BLAST of CF417147 vs. ExPASy Swiss-Prot
Match: UBIL_NPVOP (Ubiquitin-like protein OS=Orgyia pseudotsugata multicapsid polyhedrosis virus GN=V-UBI PE=3 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 1.275e-27
Identity = 60/75 (80.00%), Postives = 66/75 (88.00%), Query Frame = 1

Query:    1 QIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 225
            QIFVKTLTGKTIT+E E  DT+  VK KI DKEG+P DQQRLI+AGKQLED +TLADYNIQKESTLH+VLRLRGG
Sbjct:    2 QIFVKTLTGKTITVETEPGDTVGQVKQKIADKEGVPVDQQRLIYAGKQLEDAQTLADYNIQKESTLHMVLRLRGG 76

HSP 2 Score: 59.3066 bits (142), Expect = 3.304e-16
Identity = 27/44 (61.36%), Postives = 33/44 (75.00%), Query Frame = 2

Query:  257 KQLLLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGR 388
            K + +E E  DT+  VK KI DKEG+P DQQRLI+AGKQLED +
Sbjct:   11 KTITVETEPGDTVGQVKQKIADKEGVPVDQQRLIYAGKQLEDAQ 54

HSP 3 Score: 46.2098 bits (108), Expect = 3.304e-16
Identity = 21/22 (95.45%), Postives = 22/22 (100.00%), Query Frame = 3

Query:  390 TLADYNIQKESTLHLVLRLRGG 455
            TLADYNIQKESTLH+VLRLRGG
Sbjct:   55 TLADYNIQKESTLHMVLRLRGG 76

[Close]

BLAST of CF417147 vs. ExPASy Swiss-Prot
Match: UBIQ_LUMTE (Ubiquitin (Fragment) OS=Lumbricus terrestris PE=1 SV=2)

HSP 1 Score: 120.553 bits (301), Expect = 8.267e-27
Identity = 60/63 (95.24%), Postives = 62/63 (98.41%), Query Frame = 1

Query:   31 TITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLR 219
            TITLEVE SDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLRLR
Sbjct:    1 TITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLR 63

HSP 2 Score: 78.9518 bits (193), Expect = 1.515e-20
Identity = 38/42 (90.48%), Postives = 40/42 (95.24%), Query Frame = 2

Query:  263 LLLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGR 388
            + LEVE SDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLEDGR
Sbjct:    2 ITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGR 43

HSP 3 Score: 41.2022 bits (95), Expect = 1.515e-20
Identity = 19/20 (95.00%), Postives = 20/20 (100.00%), Query Frame = 3

Query:  390 TLADYNIQKESTLHLVLRLR 449
            TL+DYNIQKESTLHLVLRLR
Sbjct:   44 TLSDYNIQKESTLHLVLRLR 63

HSP 4 Score: 77.0258 bits (188), Expect = 1.048e-13
Identity = 44/61 (72.13%), Postives = 47/61 (77.05%), Query Frame = 1

Query:  496 TLEVESSDTIDNVKAKIQX*XRGIPPDQPEG*SLLENNLEDGRTLADYNIQKESTLHLVLR 678
            TLEVE SDTI+NVKAKIQ    GIPPDQ +        LEDGRTL+DYNIQKESTLHLVLR
Sbjct:    3 TLEVEPSDTIENVKAKIQD-KEGIPPDQ-QRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR 61

[Close]

BLAST of CF417147 vs. ExPASy Swiss-Prot
Match: UBIL_NPVAC (Ubiquitin-like protein OS=Autographa californica nuclear polyhedrosis virus GN=V-UBI PE=3 SV=2)

HSP 1 Score: 120.553 bits (301), Expect = 8.267e-27
Identity = 57/75 (76.00%), Postives = 67/75 (89.33%), Query Frame = 1

Query:    1 QIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 225
            QIF+KTLTGKTIT E E ++T+ ++K KI DKEG+P DQQRLIFAGKQLED +T+ADYNIQKESTLH+VLRLRGG
Sbjct:    2 QIFIKTLTGKTITAETEPAETVADLKQKIADKEGVPVDQQRLIFAGKQLEDSKTMADYNIQKESTLHMVLRLRGG 76

HSP 2 Score: 57.7658 bits (138), Expect = 1.596e-15
Identity = 26/44 (59.09%), Postives = 34/44 (77.27%), Query Frame = 2

Query:  257 KQLLLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGR 388
            K +  E E ++T+ ++K KI DKEG+P DQQRLIFAGKQLED +
Sbjct:   11 KTITAETEPAETVADLKQKIADKEGVPVDQQRLIFAGKQLEDSK 54

HSP 3 Score: 45.4394 bits (106), Expect = 1.596e-15
Identity = 20/22 (90.91%), Postives = 22/22 (100.00%), Query Frame = 3

Query:  390 TLADYNIQKESTLHLVLRLRGG 455
            T+ADYNIQKESTLH+VLRLRGG
Sbjct:   55 TMADYNIQKESTLHMVLRLRGG 76

[Close]

BLAST of CF417147 vs. ExPASy Swiss-Prot
Match: RUB1_DESAN (NEDD8-like protein RUB1 OS=Deschampsia antarctica GN=RUB1 PE=2 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 1.972e-20
Identity = 48/74 (64.86%), Postives = 59/74 (79.73%), Query Frame = 1

Query:    4 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 225
            I VKTLTGK I +++E +DTID VK ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRVKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

HSP 2 Score: 59.6918 bits (143), Expect = 3.193e-12
Identity = 25/44 (56.82%), Postives = 37/44 (84.09%), Query Frame = 2

Query:  257 KQLLLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGR 388
            K++ +++E +DTID VK ++++KEGIPP QQRLI+AGKQL D +
Sbjct:   11 KEIEIDIEPTDTIDRVKERVEEKEGIPPVQQRLIYAGKQLADDK 54

HSP 3 Score: 32.3426 bits (72), Expect = 3.193e-12
Identity = 15/22 (68.18%), Postives = 16/22 (72.73%), Query Frame = 3

Query:  390 TLADYNIQKESTLHLVLRLRGG 455
            T  DYNI+  S LHLVL LRGG
Sbjct:   55 TAKDYNIEGGSVLHLVLALRGG 76

[Close]

BLAST of CF417147 vs. ExPASy Swiss-Prot
Match: RUB2_ORYSJ (NEDD8-like protein RUB2 OS=Oryza sativa subsp. japonica GN=RUB2 PE=3 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 2.576e-20
Identity = 47/74 (63.51%), Postives = 59/74 (79.73%), Query Frame = 1

Query:    4 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 225
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

HSP 2 Score: 59.3066 bits (142), Expect = 4.148e-12
Identity = 24/44 (54.55%), Postives = 37/44 (84.09%), Query Frame = 2

Query:  257 KQLLLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGR 388
            K++ +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +
Sbjct:   11 KEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDK 54

HSP 3 Score: 32.3426 bits (72), Expect = 4.148e-12
Identity = 15/22 (68.18%), Postives = 16/22 (72.73%), Query Frame = 3

Query:  390 TLADYNIQKESTLHLVLRLRGG 455
            T  DYNI+  S LHLVL LRGG
Sbjct:   55 TAKDYNIEGGSVLHLVLALRGG 76

[Close]

BLAST of CF417147 vs. ExPASy Swiss-Prot
Match: RUB1_ORYSJ (NEDD8-like protein RUB1 OS=Oryza sativa subsp. japonica GN=RUB1 PE=3 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 2.576e-20
Identity = 47/74 (63.51%), Postives = 59/74 (79.73%), Query Frame = 1

Query:    4 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 225
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

HSP 2 Score: 59.3066 bits (142), Expect = 4.148e-12
Identity = 24/44 (54.55%), Postives = 37/44 (84.09%), Query Frame = 2

Query:  257 KQLLLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGR 388
            K++ +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +
Sbjct:   11 KEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDK 54

HSP 3 Score: 32.3426 bits (72), Expect = 4.148e-12
Identity = 15/22 (68.18%), Postives = 16/22 (72.73%), Query Frame = 3

Query:  390 TLADYNIQKESTLHLVLRLRGG 455
            T  DYNI+  S LHLVL LRGG
Sbjct:   55 TAKDYNIEGGSVLHLVLALRGG 76

[Close]

BLAST of CF417147 vs. ExPASy Swiss-Prot
Match: RUB1_ARATH (NEDD8-like protein RUB1 OS=Arabidopsis thaliana GN=RUB1 PE=1 SV=2)

HSP 1 Score: 98.9821 bits (245), Expect = 2.576e-20
Identity = 47/74 (63.51%), Postives = 59/74 (79.73%), Query Frame = 1

Query:    4 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 225
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

HSP 2 Score: 59.3066 bits (142), Expect = 4.148e-12
Identity = 24/44 (54.55%), Postives = 37/44 (84.09%), Query Frame = 2

Query:  257 KQLLLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGR 388
            K++ +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +
Sbjct:   11 KEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDK 54

HSP 3 Score: 32.3426 bits (72), Expect = 4.148e-12
Identity = 15/22 (68.18%), Postives = 16/22 (72.73%), Query Frame = 3

Query:  390 TLADYNIQKESTLHLVLRLRGG 455
            T  DYNI+  S LHLVL LRGG
Sbjct:   55 TAKDYNIEGGSVLHLVLALRGG 76

[Close]

BLAST of CF417147 vs. ExPASy Swiss-Prot
Match: NEDD8_DROME (NEDD8 OS=Drosophila melanogaster GN=Nedd8 PE=1 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 5.738e-20
Identity = 45/77 (58.44%), Postives = 59/77 (76.62%), Query Frame = 1

Query:    4 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQI 234
            I VKTLTGK I +++E +D +D +K ++++KEGIPP QQRLIF+GKQ+ D +T ADY +Q  S LHLVL LRGG  I
Sbjct:    3 IKVKTLTGKEIEIDIEPTDKVDRIKERVEEKEGIPPQQQRLIFSGKQMNDDKTAADYKVQGGSVLHLVLALRGGDSI 79

HSP 2 Score: 57.3806 bits (137), Expect = 9.094e-12
Identity = 21/44 (47.73%), Postives = 36/44 (81.82%), Query Frame = 2

Query:  257 KQLLLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGR 388
            K++ +++E +D +D +K ++++KEGIPP QQRLIF+GKQ+ D +
Sbjct:   11 KEIEIDIEPTDKVDRIKERVEEKEGIPPQQQRLIFSGKQMNDDK 54

HSP 3 Score: 33.113 bits (74), Expect = 9.094e-12
Identity = 16/25 (64.00%), Postives = 17/25 (68.00%), Query Frame = 3

Query:  390 TLADYNIQKESTLHLVLRLRGGMQI 464
            T ADY +Q  S LHLVL LRGG  I
Sbjct:   55 TAADYKVQGGSVLHLVLALRGGDSI 79

The following BLAST results are available for this feature:

BLAST of CF417147 vs. ExPASy Swiss-Prot

BLAST of CF417147 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 92


Match Name	E-value	Identity	Description
UBIQ_LEIMA	7.720e-33	93.33	Ubiquitin OS=Leishmania major GN=UB-EP52 PE=3 SV=1	[more]
UBIQ_LEITA	2.483e-31	90.67	Ubiquitin OS=Leishmania tarentolae GN=UB-EP52 PE=3...	[more]
UBIL_NPVOP	1.275e-27	80.00	Ubiquitin-like protein OS=Orgyia pseudotsugata mul...	[more]
UBIQ_LUMTE	8.267e-27	95.24	Ubiquitin (Fragment) OS=Lumbricus terrestris PE=1 ...	[more]
UBIL_NPVAC	8.267e-27	76.00	Ubiquitin-like protein OS=Autographa californica n...	[more]
RUB1_DESAN	1.972e-20	64.86	NEDD8-like protein RUB1 OS=Deschampsia antarctica ...	[more]
RUB2_ORYSJ	2.576e-20	63.51	NEDD8-like protein RUB2 OS=Oryza sativa subsp. jap...	[more]
RUB1_ORYSJ	2.576e-20	63.51	NEDD8-like protein RUB1 OS=Oryza sativa subsp. jap...	[more]
RUB1_ARATH	2.576e-20	63.51	NEDD8-like protein RUB1 OS=Arabidopsis thaliana GN...	[more]
NEDD8_DROME	5.738e-20	58.44	NEDD8 OS=Drosophila melanogaster GN=Nedd8 PE=1 SV=...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_115000-077 Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange Citrus sinensis cDNA clone IDFL-001_H10 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF417147 ID=CF417147; Name=CF417147; organism=Citrus sinensis; type=EST; length=694bp
CAGATTTTTGTGAAAACCCTGACTGGTAAGACTATAACTTTGGAGGTTGA
AAGCTCTGACACGATCGACAATGTCAAGGCCAAGATCCAGGACAAGGAGG
GCATCCCACCTGATCAACAGAGGTTAATCTTTGCCGGCAAACAACTTGAA
GATGGTCGTACTCTTGCTGACTACAACATCCAGAAGGAGTCAACCCTCCA
TTTGGTGCTTCGTCTCCGTGGTGGCATGCAGATCTTTGTGAAAACCCTCA
CCGGAAAAACAATTACTCTTGGAGGTTGAAAGTTCTGACACCATCGACAA
CGTGAAAGCAAAAATTCAAGACAAGGAGGGTATCCCACCAGATCAGCAGA
GGCTCATTTTTGCTGGAAAGCAGCTTGAAGATGGAAGGGACTCTTGCAGA
CTATAACATCCAGAAAGAATCCACTCTTCACTTGGTTCTTCGCCTCCGTG
GTGGTATGCAGATTTTTGTGAAGACACTCACTGGGAAAACCATCCACATT
GGAGGTTGAGAGTTCGGACACAATTGACAATGTGAAGGCTAAGATCCAGN
GATAANGGAGGGGGATTCCCCCAGACCAGCCAGAAGGTTGATCTTTGCTG
GAAAACAACTTGGAGGATGGAAGGACTCTTGCTGATTACAACATCCAGAA
GGAGTCCACTCTGCATCTTGTGCTCCGTCCTCCCGTGGGAGGTT