CF417147 | Citrus Genome Database

Overview


Name	CF417147
Unique Name	CF417147
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	694

Libraries


Library Name	Type
Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CF417147 vs. ExPASy Swiss-Prot
Match: RUB2_ARATH (NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN=RUB2 PE=2 SV=2)

HSP 1 Score: 96.6709 bits (239), Expect = 1.278e-19
Identity = 46/75 (61.33%), Postives = 59/75 (78.67%), Query Frame = 1

Query:    4 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 228
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DY I+  S LHLVL LRGG+
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYAIEGGSVLHLVLALRGGL 77

HSP 2 Score: 59.3066 bits (142), Expect = 1.993e-11
Identity = 24/44 (54.55%), Postives = 37/44 (84.09%), Query Frame = 2

Query:  257 KQLLLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGR 388
            K++ +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +
Sbjct:   11 KEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDK 54

HSP 3 Score: 30.0314 bits (66), Expect = 1.993e-11
Identity = 14/23 (60.87%), Postives = 16/23 (69.57%), Query Frame = 3

Query:  390 TLADYNIQKESTLHLVLRLRGGM 458
            T  DY I+  S LHLVL LRGG+
Sbjct:   55 TAKDYAIEGGSVLHLVLALRGGL 77

[Close]

BLAST of CF417147 vs. ExPASy Swiss-Prot
Match: NEDD8_CAEEL (NEDD8 OS=Caenorhabditis elegans GN=ned-8 PE=1 SV=1)

HSP 1 Score: 95.9005 bits (237), Expect = 2.181e-19
Identity = 45/74 (60.81%), Postives = 57/74 (77.03%), Query Frame = 1

Query:    4 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 225
            I VKTLTGK I L++E +D ++ +K K+++KEGIPP QQRLIFAGKQ+ D +T ADY +   S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIELDIEPNDRVERIKEKVEEKEGIPPPQQRLIFAGKQMNDDKTAADYKVLGGSVLHLVLALRGG 76

HSP 2 Score: 58.5362 bits (140), Expect = 3.363e-11
Identity = 23/44 (52.27%), Postives = 36/44 (81.82%), Query Frame = 2

Query:  257 KQLLLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGR 388
            K++ L++E +D ++ +K K+++KEGIPP QQRLIFAGKQ+ D +
Sbjct:   11 KEIELDIEPNDRVERIKEKVEEKEGIPPPQQRLIFAGKQMNDDK 54

HSP 3 Score: 30.0314 bits (66), Expect = 3.363e-11
Identity = 14/22 (63.64%), Postives = 15/22 (68.18%), Query Frame = 3

Query:  390 TLADYNIQKESTLHLVLRLRGG 455
            T ADY +   S LHLVL LRGG
Sbjct:   55 TAADYKVLGGSVLHLVLALRGG 76

[Close]

BLAST of CF417147 vs. ExPASy Swiss-Prot
Match: RUB3_ORYSJ (NEDD8-like protein RUB3 OS=Oryza sativa subsp. japonica GN=RUB3 PE=3 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 3.149e-18
Identity = 41/72 (56.94%), Postives = 56/72 (77.78%), Query Frame = 1

Query:   10 VKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 225
            V+TLTGK I +++E +DT+D +K +++++EGIPP QQRLI+ GKQL D +T  DY I+  S LHLVL LRGG
Sbjct:    5 VRTLTGKEIDIDIEMTDTVDRIKERVEEREGIPPVQQRLIYGGKQLADDKTAHDYKIEAGSVLHLVLALRGG 76

[Close]

BLAST of CF417147 vs. ExPASy Swiss-Prot
Match: NEDD8_RAT (NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 3.149e-18
Identity = 42/74 (56.76%), Postives = 57/74 (77.03%), Query Frame = 1

Query:    4 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 225
            I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

[Close]

BLAST of CF417147 vs. ExPASy Swiss-Prot
Match: NEDD8_RABIT (NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 3.149e-18
Identity = 42/74 (56.76%), Postives = 57/74 (77.03%), Query Frame = 1

Query:    4 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 225
            I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

[Close]

BLAST of CF417147 vs. ExPASy Swiss-Prot
Match: NEDD8_MOUSE (NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2)

HSP 1 Score: 92.0485 bits (227), Expect = 3.149e-18
Identity = 42/74 (56.76%), Postives = 57/74 (77.03%), Query Frame = 1

Query:    4 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 225
            I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

[Close]

BLAST of CF417147 vs. ExPASy Swiss-Prot
Match: NEDD8_HUMAN (NEDD8 OS=Homo sapiens GN=NEDD8 PE=1 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 3.149e-18
Identity = 42/74 (56.76%), Postives = 57/74 (77.03%), Query Frame = 1

Query:    4 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 225
            I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

[Close]

BLAST of CF417147 vs. ExPASy Swiss-Prot
Match: NEDD8_BOVIN (NEDD8 OS=Bos taurus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 3.149e-18
Identity = 42/74 (56.76%), Postives = 57/74 (77.03%), Query Frame = 1

Query:    4 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 225
            I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

[Close]

BLAST of CF417147 vs. ExPASy Swiss-Prot
Match: NEDD8_DICDI (NEDD8 OS=Dictyostelium discoideum GN=nedd8 PE=3 SV=2)

HSP 1 Score: 88.5817 bits (218), Expect = 3.481e-17
Identity = 40/75 (53.33%), Postives = 57/75 (76.00%), Query Frame = 1

Query:    4 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 228
            I VKTLTGK I ++++ +D I  +K ++++KEGIPP QQRLIF GKQ+ D +  ++Y+I+  S LHLVL LRGG+
Sbjct:    3 IKVKTLTGKEIEIDIDPTDKIQRIKERVEEKEGIPPSQQRLIFGGKQMGDDKPASEYSIEGGSVLHLVLALRGGL 77

[Close]

BLAST of CF417147 vs. ExPASy Swiss-Prot
Match: RUB3_ARATH (NEDD8-like protein RUB3 OS=Arabidopsis thaliana GN=RUB3 PE=2 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 4.547e-17
Identity = 41/75 (54.67%), Postives = 56/75 (74.67%), Query Frame = 1

Query:    1 QIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 225
            +I VKTLT K I +E+E +DTI+ +K +I++KEGIPP  QR+++ GKQL D  T   YN+++ S LHLVL LRGG
Sbjct:    2 EIKVKTLTEKQIDIEIELTDTIERIKERIEEKEGIPPVHQRIVYTGKQLADDLTAKHYNLERGSVLHLVLALRGG 76

HSP 2 Score: 56.9954 bits (136), Expect = 7.367e-11
Identity = 24/47 (51.06%), Postives = 36/47 (76.60%), Query Frame = 2

Query:  242 KPSPEKQLLLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLED 382
            K   EKQ+ +E+E +DTI+ +K +I++KEGIPP  QR+++ GKQL D
Sbjct:    6 KTLTEKQIDIEIELTDTIERIKERIEEKEGIPPVHQRIVYTGKQLAD 52

HSP 3 Score: 30.4166 bits (67), Expect = 7.367e-11
Identity = 13/22 (59.09%), Postives = 16/22 (72.73%), Query Frame = 3

Query:  390 TLADYNIQKESTLHLVLRLRGG 455
            T   YN+++ S LHLVL LRGG
Sbjct:   55 TAKHYNLERGSVLHLVLALRGG 76

The following BLAST results are available for this feature:

BLAST of CF417147 vs. ExPASy Swiss-Prot

BLAST of CF417147 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 92


Match Name	E-value	Identity	Description
RUB2_ARATH	1.278e-19	61.33	NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN...	[more]
NEDD8_CAEEL	2.181e-19	60.81	NEDD8 OS=Caenorhabditis elegans GN=ned-8 PE=1 SV=1	[more]
RUB3_ORYSJ	3.149e-18	56.94	NEDD8-like protein RUB3 OS=Oryza sativa subsp. jap...	[more]
NEDD8_RAT	3.149e-18	56.76	NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1	[more]
NEDD8_RABIT	3.149e-18	56.76	NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1	[more]
NEDD8_MOUSE	3.149e-18	56.76	NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2	[more]
NEDD8_HUMAN	3.149e-18	56.76	NEDD8 OS=Homo sapiens GN=NEDD8 PE=1 SV=1	[more]
NEDD8_BOVIN	3.149e-18	56.76	NEDD8 OS=Bos taurus GN=NEDD8 PE=3 SV=1	[more]
NEDD8_DICDI	3.481e-17	53.33	NEDD8 OS=Dictyostelium discoideum GN=nedd8 PE=3 SV...	[more]
RUB3_ARATH	4.547e-17	54.67	NEDD8-like protein RUB3 OS=Arabidopsis thaliana GN...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_115000-077 Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange Citrus sinensis cDNA clone IDFL-001_H10 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF417147 ID=CF417147; Name=CF417147; organism=Citrus sinensis; type=EST; length=694bp
CAGATTTTTGTGAAAACCCTGACTGGTAAGACTATAACTTTGGAGGTTGA
AAGCTCTGACACGATCGACAATGTCAAGGCCAAGATCCAGGACAAGGAGG
GCATCCCACCTGATCAACAGAGGTTAATCTTTGCCGGCAAACAACTTGAA
GATGGTCGTACTCTTGCTGACTACAACATCCAGAAGGAGTCAACCCTCCA
TTTGGTGCTTCGTCTCCGTGGTGGCATGCAGATCTTTGTGAAAACCCTCA
CCGGAAAAACAATTACTCTTGGAGGTTGAAAGTTCTGACACCATCGACAA
CGTGAAAGCAAAAATTCAAGACAAGGAGGGTATCCCACCAGATCAGCAGA
GGCTCATTTTTGCTGGAAAGCAGCTTGAAGATGGAAGGGACTCTTGCAGA
CTATAACATCCAGAAAGAATCCACTCTTCACTTGGTTCTTCGCCTCCGTG
GTGGTATGCAGATTTTTGTGAAGACACTCACTGGGAAAACCATCCACATT
GGAGGTTGAGAGTTCGGACACAATTGACAATGTGAAGGCTAAGATCCAGN
GATAANGGAGGGGGATTCCCCCAGACCAGCCAGAAGGTTGATCTTTGCTG
GAAAACAACTTGGAGGATGGAAGGACTCTTGCTGATTACAACATCCAGAA
GGAGTCCACTCTGCATCTTGTGCTCCGTCCTCCCGTGGGAGGTT