CF417240

Overview
NameCF417240
Unique NameCF417240
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length681
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig4591 contig Csv1_Contig4591:178..859. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF417240 vs. ExPASy Swiss-Prot
Match: UBE2N_MACFA (Ubiquitin-conjugating enzyme E2 N OS=Macaca fascicularis GN=UBE2N PE=2 SV=1)

HSP 1 Score: 159.073 bits (401), Expect = 2.009e-38
Identity = 73/92 (79.35%), Postives = 83/92 (90.22%), Query Frame = -3 
Query:  272 FCLKNXPMAAPKVRFLTKIYHPNIDKLGRICLDIXKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKEWTRLYA 547
            F  +  PMAAPKVRF+TKIYHPN+DK GRICLDI KDKWSPALQIRTVLLSIQALLSAPNPDDPL+ ++A+ WKTNEA+A+ETA+ WTRLYA
Sbjct:   57 FLPEEYPMAAPKVRFMTKIYHPNVDKSGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQAIETARAWTRLYA 148

HSP 2 Score: 80.8777 bits (198), Expect = 6.952e-15
Identity = 37/56 (66.07%), Postives = 41/56 (73.21%), Query Frame = -2 
Query:  513 QRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYXNGCSK 680
            QRLL+EP PGI A P E N RYF+V+I GP  SP+EGG FKLELFLPEEY     K
Sbjct:   13 QRLLAEPVPGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPMAAPK 68
[Close]
BLAST of CF417240 vs. ExPASy Swiss-Prot
Match: UBE2N_DROME (Ubiquitin-conjugating enzyme E2 N OS=Drosophila melanogaster GN=ben PE=2 SV=1)

HSP 1 Score: 151.754 bits (382), Expect = 3.207e-36
Identity = 68/92 (73.91%), Postives = 82/92 (89.13%), Query Frame = -3 
Query:  272 FCLKNXPMAAPKVRFLTKIYHPNIDKLGRICLDIXKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKEWTRLYA 547
            F  ++ PM+APKVRF+TKIYHPNID+LGRICLD+ KDKWSPALQIRT+LLSIQALLSAPNPDDPL+ ++A+ WK NEAEA+  A+EWT+ YA
Sbjct:   57 FLPEDYPMSAPKVRFITKIYHPNIDRLGRICLDVLKDKWSPALQIRTILLSIQALLSAPNPDDPLANDVAELWKVNEAEAIRNAREWTQKYA 148

HSP 2 Score: 80.1073 bits (196), Expect = 1.186e-14
Identity = 35/56 (62.50%), Postives = 43/56 (76.79%), Query Frame = -2 
Query:  513 QRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYXNGCSK 680
            QRL+ EP PGI+A P E+N RYF+V++ GP  SP+EGGVFKLELFLPE+Y     K
Sbjct:   13 QRLMQEPVPGINAIPDENNARYFHVIVTGPNDSPFEGGVFKLELFLPEDYPMSAPK 68
[Close]
BLAST of CF417240 vs. ExPASy Swiss-Prot
Match: UBC13_YEAST (Ubiquitin-conjugating enzyme E2 13 OS=Saccharomyces cerevisiae GN=UBC13 PE=1 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 3.106e-31
Identity = 63/101 (62.38%), Postives = 78/101 (77.23%), Query Frame = -3 
Query:  272 KEGCSNWNYFCLKNXPMAAPKVRFLTKIYHPNIDKLGRICLDIXKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKEWTRLYA 574
            ++G      +   + PM APKVRFLTKIYHPNID+LGRICLD+ K  WSPALQIRTVLLSIQALL++PNP+DPL+ ++A+ W  NE  A   A+EWT+LYA
Sbjct:   48 EDGIFELELYLPDDYPMEAPKVRFLTKIYHPNIDRLGRICLDVLKTNWSPALQIRTVLLSIQALLASPNPNDPLANDVAEDWIKNEQGAKAKAREWTKLYA 148

HSP 2 Score: 74.3294 bits (181), Expect = 6.507e-13
Identity = 30/50 (60.00%), Postives = 42/50 (84.00%), Query Frame = -2 
Query:  531 QRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEY 680
            ++L+S+P PGI+A P +DN+RYF V I GP QSPYE G+F+LEL+LP++Y
Sbjct:   13 EKLVSDPVPGITAEPHDDNLRYFQVTIEGPEQSPYEDGIFELELYLPDDY 62
[Close]
BLAST of CF417240 vs. ExPASy Swiss-Prot
Match: UBC5_YEAST (Ubiquitin-conjugating enzyme E2-16 kDa OS=Saccharomyces cerevisiae GN=UBC5 PE=1 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 7.008e-30
Identity = 52/86 (60.47%), Postives = 63/86 (73.26%), Query Frame = -3 
Query:  272 PMAAPKVRFLTKIYHPNIDKLGRICLDIXKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKEWTRLYA 529
            P   PKV F TKIYHPNI+  G ICLDI KD+WSPAL +  VLLSI +LL+  NPDDPL   IA+ +KT++A+   TAKEWT+ YA
Sbjct:   62 PFKPPKVNFTTKIYHPNINSSGNICLDILKDQWSPALTLSKVLLSICSLLTDANPDDPLVPEIAQIYKTDKAKYEATAKEWTKKYA 147

HSP 2 Score: 43.8986 bits (102), Expect = 7.008e-30
Identity = 18/45 (40.00%), Postives = 27/45 (60.00%), Query Frame = -2 
Query:  531 EPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEY 665
            +P    SA P  D++ ++   I+GP+ SPY GGVF L +  P +Y
Sbjct:   17 DPPASCSAGPVGDDLYHWQASIMGPSDSPYAGGVFFLSIHFPTDY 61
[Close]
BLAST of CF417240 vs. ExPASy Swiss-Prot
Match: UBC4_SOLLC (Ubiquitin-conjugating enzyme E2-17 kDa OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 3.416e-29
Identity = 50/88 (56.82%), Postives = 62/88 (70.45%), Query Frame = -3 
Query:  266 PMAAPKVRFLTKIYHPNIDKLGRICLDIXKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKEWTRLYASG 529
            P   PKV F TK++HPNI+  G ICLDI K++WSPAL I  VLLSI +LL+ PNPDDPL   IA  +KT+ A+   TA+ WT+ YA G
Sbjct:   61 PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYETTARSWTQKYAMG 148

HSP 2 Score: 45.0542 bits (105), Expect = 3.416e-29
Identity = 19/48 (39.58%), Postives = 28/48 (58.33%), Query Frame = -2 
Query:  531 LLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEY 674
            L  +P    SA P  ++M ++   I+GPT SPY GGVF + +  P +Y
Sbjct:   13 LQKDPPTSCSAGPVAEDMFHWQATIMGPTDSPYAGGVFLVSIHFPPDY 60
[Close]
BLAST of CF417240 vs. ExPASy Swiss-Prot
Match: UBC11_ARATH (Ubiquitin-conjugating enzyme E2 11 OS=Arabidopsis thaliana GN=UBC11 PE=2 SV=2)

HSP 1 Score: 104.76 bits (260), Expect = 5.791e-29
Identity = 50/88 (56.82%), Postives = 62/88 (70.45%), Query Frame = -3 
Query:  266 PMAAPKVRFLTKIYHPNIDKLGRICLDIXKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKEWTRLYASG 529
            P   PKV F TK+YHPNI+  G ICLDI K++WSPAL I  VLLSI +LL+ PNPDDPL   IA  +KT+ ++   TA+ WT+ YA G
Sbjct:   61 PFKPPKVSFKTKVYHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRSKYESTARSWTQKYAMG 148

HSP 2 Score: 43.5134 bits (101), Expect = 5.791e-29
Identity = 18/48 (37.50%), Postives = 28/48 (58.33%), Query Frame = -2 
Query:  531 LLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEY 674
            L  +P    SA P  ++M ++   I+GP +SPY GGVF + +  P +Y
Sbjct:   13 LQKDPPSNCSAGPVAEDMFHWQATIMGPPESPYAGGVFLVSIHFPPDY 60
[Close]
BLAST of CF417240 vs. ExPASy Swiss-Prot
Match: UBE2T_HUMAN (Ubiquitin-conjugating enzyme E2 T OS=Homo sapiens GN=UBE2T PE=1 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 7.467e-29
Identity = 46/90 (51.11%), Postives = 60/90 (66.67%), Query Frame = -3 
Query:  272 PMAAPKVRFLTKIYHPNIDKLGRICLDIXK----DKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKEWTRLYA 529
            P   P++RFLT IYHPNID  GRICLD+ K      W P+L I TVL SIQ L+S PNPDDPL  +I+  +K N+   ++ A++WT  +A
Sbjct:   62 PFEPPQIRFLTPIYHPNIDSAGRICLDVLKLPPKGAWRPSLNIATVLTSIQLLMSEPNPDDPLMADISSEFKYNKPAFLKNARQWTEKHA 151

HSP 2 Score: 50.0618 bits (118), Expect = 7.467e-29
Identity = 23/48 (47.92%), Postives = 29/48 (60.42%), Query Frame = -2 
Query:  531 LLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEY 674
            L +EP PGI+    +D M      ILG   +PYE GVFKLE+ +PE Y
Sbjct:   14 LATEPPPGITCWQDKDQMDDLRAQILGGANTPYEKGVFKLEVIIPERY 61
[Close]
BLAST of CF417240 vs. ExPASy Swiss-Prot
Match: UBC8_ARATH (Ubiquitin-conjugating enzyme E2 8 OS=Arabidopsis thaliana GN=UBC8 PE=1 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 7.541e-29
Identity = 50/88 (56.82%), Postives = 62/88 (70.45%), Query Frame = -3 
Query:  266 PMAAPKVRFLTKIYHPNIDKLGRICLDIXKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKEWTRLYASG 529
            P   PKV F TK++HPNI+  G ICLDI K++WSPAL I  VLLSI +LL+ PNPDDPL   IA  +KT+ A+   TA+ WT+ YA G
Sbjct:   61 PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYEATARNWTQKYAMG 148

HSP 2 Score: 43.8986 bits (102), Expect = 7.541e-29
Identity = 18/48 (37.50%), Postives = 28/48 (58.33%), Query Frame = -2 
Query:  531 LLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEY 674
            L  +P    SA P  ++M ++   I+GP +SPY GGVF + +  P +Y
Sbjct:   13 LQKDPPTSCSAGPVAEDMFHWQATIMGPAESPYSGGVFLVTIHFPPDY 60
[Close]
BLAST of CF417240 vs. ExPASy Swiss-Prot
Match: UBC1_MAGGR (Ubiquitin-conjugating enzyme E2-16 kDa OS=Magnaporthe grisea GN=UBC1 PE=3 SV=2)

HSP 1 Score: 105.916 bits (263), Expect = 9.818e-29
Identity = 51/86 (59.30%), Postives = 61/86 (70.93%), Query Frame = -3 
Query:  272 PMAAPKVRFLTKIYHPNIDKLGRICLDIXKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKEWTRLYA 529
            P   PKV F T+IYHPNI+  G ICLDI +D+WSPAL I  VLLSI ++L+ PNPDDPL   IA  +KT  A+   TA+EWTR YA
Sbjct:   61 PFKPPKVNFTTRIYHPNINSNGSICLDILRDQWSPALTISKVLLSICSMLTDPNPDDPLVPEIAHVYKTARAQYESTAREWTRKYA 146

HSP 2 Score: 41.5874 bits (96), Expect = 9.818e-29
Identity = 17/45 (37.78%), Postives = 27/45 (60.00%), Query Frame = -2 
Query:  531 EPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEY 665
            +P    SA P  +++ ++   I+GP+ SPY GGVF L +  P +Y
Sbjct:   16 DPPSSCSAGPVGEDLFHWQATIMGPSDSPYAGGVFFLAIHFPTDY 60
[Close]
BLAST of CF417240 vs. ExPASy Swiss-Prot
Match: UBC28_ARATH (Ubiquitin carrier protein E2 28 OS=Arabidopsis thaliana GN=UBC28 PE=2 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 9.818e-29
Identity = 49/88 (55.68%), Postives = 62/88 (70.45%), Query Frame = -3 
Query:  266 PMAAPKVRFLTKIYHPNIDKLGRICLDIXKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKEWTRLYASG 529
            P   PKV F TK++HPN++  G ICLDI K++WSPAL I  VLLSI +LL+ PNPDDPL   IA  +KT+ A+   TA+ WT+ YA G
Sbjct:   61 PFKPPKVAFRTKVFHPNVNSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYESTARSWTQKYAMG 148

HSP 2 Score: 43.5134 bits (101), Expect = 9.818e-29
Identity = 18/48 (37.50%), Postives = 28/48 (58.33%), Query Frame = -2 
Query:  531 LLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEY 674
            L  +P    SA P  ++M ++   I+GP+ SPY GGVF + +  P +Y
Sbjct:   13 LQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVTIHFPPDY 60
The following BLAST results are available for this feature:
BLAST of CF417240 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 199
Match NameE-valueIdentityDescription
UBE2N_MACFA2.009e-3879.35Ubiquitin-conjugating enzyme E2 N OS=Macaca fascic... [more]
UBE2N_DROME3.207e-3673.91Ubiquitin-conjugating enzyme E2 N OS=Drosophila me... [more]
UBC13_YEAST3.106e-3162.38Ubiquitin-conjugating enzyme E2 13 OS=Saccharomyce... [more]
UBC5_YEAST7.008e-3060.47Ubiquitin-conjugating enzyme E2-16 kDa OS=Saccharo... [more]
UBC4_SOLLC3.416e-2956.82Ubiquitin-conjugating enzyme E2-17 kDa OS=Solanum ... [more]
UBC11_ARATH5.791e-2956.82Ubiquitin-conjugating enzyme E2 11 OS=Arabidopsis ... [more]
UBE2T_HUMAN7.467e-2951.11Ubiquitin-conjugating enzyme E2 T OS=Homo sapiens ... [more]
UBC8_ARATH7.541e-2956.82Ubiquitin-conjugating enzyme E2 8 OS=Arabidopsis t... [more]
UBC1_MAGGR9.818e-2959.30Ubiquitin-conjugating enzyme E2-16 kDa OS=Magnapor... [more]
UBC28_ARATH9.818e-2955.68Ubiquitin carrier protein E2 28 OS=Arabidopsis tha... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_115000-170 Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange Citrus sinensis cDNA clone IDFL-003_A07 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF417240 ID=CF417240; Name=CF417240; organism=Citrus sinensis; type=EST; length=681bp
GTTTTTTTTTTTTTTTTTTTTTGCTTCGAAATTTAAGAGTTGAGAAACAA
TGTATATCATCTAATCATACAAGTTCAAAGACATGGCTTCCACAAGAAGA
AAATAATTGACATGTGCGGTAAGATGGAAGCTTTCAGAATGTCAAACTCC
GAATGTTCAAACATCAATTCAATTCTTCCAAATTTTCCATTTTAAACACA
GTGAATAGCATACATTGCACAATTGTTATTTGAATGAAGTGATATTTTCC
ACCATGTCATCAAGCACCACTGGCATATAAACGTGTCCATTCCTTCGCTG
TTTCAACCGCTTCGGCCTCATTTGTTTTCCAGTGTTTGGCAATGTTCTCA
GAGAGAGGATCATCGGGGTTAGGAGCACTCAGAAGTGCTTGAATGCTCAA
AAGTACAGTCCGAATCTGGAGAGCTGGACTCCACTTGTCTTTCANAATAT
CAAGACAAATCCTTCCAAGCTTATCAATGTTAGGATGATATATCTTGGTA
AGAAATCGGACCTTTGGAGCAGCCATTGGNATATTCTTCAGGCAAAAATA
ATTCCAATTTGAACACCCCTCCTTCATATGGAGATTGTGTTGGACCAAGG
ATCATCACATTAAAGTATCGCATATTATCTTCCGAGGGAGATGCACTTAT
TCCTGGTGCTGGTTCACTGAGGAGACGCTGT
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