CF417240 | Citrus Genome Database

Overview


Name	CF417240
Unique Name	CF417240
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	681

Libraries


Library Name	Type
Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig4591	contig	Csv1_Contig4591:178..859.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CF417240 vs. ExPASy Swiss-Prot
Match: UBE2K_MOUSE (Ubiquitin-conjugating enzyme E2 K OS=Mus musculus GN=Ube2k PE=2 SV=3)

HSP 1 Score: 95.9005 bits (237), Expect = 1.061e-23
Identity = 46/87 (52.87%), Postives = 60/87 (68.97%), Query Frame = -3

Query:  272 PMAAPKVRFLTKIYHPNIDKL-GRICLDIXKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKEWTRLYA 529
            P   PKVRF+TKI+HPNI  + G ICLDI KD+W+ A+ +RTVLLS+QALL+A  PDDP    +A  +K N     +TA+ W  +YA
Sbjct:   67 PFNPPKVRFITKIWHPNISSVTGAICLDILKDQWAAAMTLRTVLLSLQALLAAAEPDDPQDAVVANQYKQNPEMFKQTARLWAHVYA 153

HSP 2 Score: 34.6538 bits (78), Expect = 1.061e-23
Identity = 14/34 (41.18%), Postives = 21/34 (61.76%), Query Frame = -2

Query:  531 EDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEY 632
            ++N       I GP  +PYEGG ++LE+ +PE Y
Sbjct:   33 DENFTELRGEIAGPPDTPYEGGRYQLEIKIPETY 66

[Close]

BLAST of CF417240 vs. ExPASy Swiss-Prot
Match: UBE2K_HUMAN (Ubiquitin-conjugating enzyme E2 K OS=Homo sapiens GN=UBE2K PE=1 SV=3)

HSP 1 Score: 95.9005 bits (237), Expect = 1.061e-23
Identity = 46/87 (52.87%), Postives = 60/87 (68.97%), Query Frame = -3

Query:  272 PMAAPKVRFLTKIYHPNIDKL-GRICLDIXKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKEWTRLYA 529
            P   PKVRF+TKI+HPNI  + G ICLDI KD+W+ A+ +RTVLLS+QALL+A  PDDP    +A  +K N     +TA+ W  +YA
Sbjct:   67 PFNPPKVRFITKIWHPNISSVTGAICLDILKDQWAAAMTLRTVLLSLQALLAAAEPDDPQDAVVANQYKQNPEMFKQTARLWAHVYA 153

HSP 2 Score: 34.6538 bits (78), Expect = 1.061e-23
Identity = 14/34 (41.18%), Postives = 21/34 (61.76%), Query Frame = -2

Query:  531 EDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEY 632
            ++N       I GP  +PYEGG ++LE+ +PE Y
Sbjct:   33 DENFTELRGEIAGPPDTPYEGGRYQLEIKIPETY 66

[Close]

BLAST of CF417240 vs. ExPASy Swiss-Prot
Match: UBE2K_BOVIN (Ubiquitin-conjugating enzyme E2 K OS=Bos taurus GN=UBE2K PE=1 SV=3)

HSP 1 Score: 95.9005 bits (237), Expect = 1.061e-23
Identity = 46/87 (52.87%), Postives = 60/87 (68.97%), Query Frame = -3

Query:  272 PMAAPKVRFLTKIYHPNIDKL-GRICLDIXKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKEWTRLYA 529
            P   PKVRF+TKI+HPNI  + G ICLDI KD+W+ A+ +RTVLLS+QALL+A  PDDP    +A  +K N     +TA+ W  +YA
Sbjct:   67 PFNPPKVRFITKIWHPNISSVTGAICLDILKDQWAAAMTLRTVLLSLQALLAAAEPDDPQDAVVANQYKQNPEMFKQTARLWAHVYA 153

HSP 2 Score: 34.6538 bits (78), Expect = 1.061e-23
Identity = 14/34 (41.18%), Postives = 21/34 (61.76%), Query Frame = -2

Query:  531 EDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEY 632
            ++N       I GP  +PYEGG ++LE+ +PE Y
Sbjct:   33 DENFTELRGEIAGPPDTPYEGGRYQLEIKIPETY 66

[Close]

BLAST of CF417240 vs. ExPASy Swiss-Prot
Match: UBC1_YEAST (Ubiquitin-conjugating enzyme E2-24 kDa OS=Saccharomyces cerevisiae GN=UBC1 PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 3.952e-23
Identity = 42/88 (47.73%), Postives = 60/88 (68.18%), Query Frame = -3

Query:  269 PMAAPKVRFLTKIYHPNIDKL-GRICLDIXKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKEWTRLYAS 529
            P   PK++F TK+YHPNI  + G ICLDI K+ WSP + +++ L+S+QALL +P P+DP    +A+H+  +     +TA  WTRLYAS
Sbjct:   63 PFKPPKMQFDTKVYHPNISSVTGAICLDILKNAWSPVITLKSALISLQALLQSPEPNDPQDAEVAQHYLRDRESFNKTAALWTRLYAS 150

HSP 2 Score: 35.4242 bits (80), Expect = 3.952e-23
Identity = 18/51 (35.29%), Postives = 29/51 (56.86%), Query Frame = -2

Query:  531 QRLLSEPAPGISAS-PSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEY 680
            Q +  +PA  I+    SE ++ +     LGP  +PYEGG F +++ +P EY
Sbjct:   12 QAVKDDPAAHITLEFVSESDIHHLKGTFLGPPGTPYEGGKFVVDIEVPMEY 62

[Close]

BLAST of CF417240 vs. ExPASy Swiss-Prot
Match: UBCD4_DROME (Ubiquitin-conjugating enzyme E2-22 kDa OS=Drosophila melanogaster GN=UbcD4 PE=1 SV=2)

HSP 1 Score: 94.7449 bits (234), Expect = 3.960e-23
Identity = 47/89 (52.81%), Postives = 59/89 (66.29%), Query Frame = -3

Query:  266 PMAAPKVRFLTKIYHPNIDKL-GRICLDIXKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKEWTRLYASG 529
            P   PKVRF+T+I+HPNI  + G ICLDI KD W+ A+ +RTVLLS+QALL+A  PDDP    +A  +K      + TAK WT  YA G
Sbjct:   67 PFNPPKVRFITRIWHPNISSVTGAICLDILKDNWAAAMTLRTVLLSLQALLAAAEPDDPQDAVVAYQFKDKYDLFLLTAKHWTNAYAGG 155

HSP 2 Score: 33.8834 bits (76), Expect = 3.960e-23
Identity = 14/24 (58.33%), Postives = 17/24 (70.83%), Query Frame = -2

Query:  531 ILGPTQSPYEGGVFKLELFLPEEY 602
            I GP  +PYEGG F LE+ +PE Y
Sbjct:   43 IAGPPDTPYEGGKFVLEIKVPETY 66

[Close]

BLAST of CF417240 vs. ExPASy Swiss-Prot
Match: UBC2_YEAST (Ubiquitin-conjugating enzyme E2 2 OS=Saccharomyces cerevisiae GN=RAD6 PE=1 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 6.740e-23
Identity = 30/80 (37.50%), Postives = 52/80 (65.00%), Query Frame = -3

Query:  290 PMAAPKVRFLTKIYHPNIDKLGRICLDIXKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKE 529
            P   P V+FL++++HPN+   G ICLDI +++W+P   + ++L SIQ+L + PNP  P +   A  +K ++++ V+  KE
Sbjct:   64 PNKPPHVKFLSEMFHPNVYANGEICLDILQNRWTPTYDVASILTSIQSLFNDPNPASPANVEAATLFKDHKSQYVKRVKE 143

HSP 2 Score: 56.9954 bits (136), Expect = 6.740e-23
Identity = 25/52 (48.08%), Postives = 34/52 (65.38%), Query Frame = -2

Query:  525 QRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYXN 680
            +R+  +  PG+SASP  DN+  +N MI+GP  +PYE G F+L L   EEY N
Sbjct:   14 KRMKEDAPPGVSASPLPDNVMVWNAMIIGPADTPYEDGTFRLLLEFDEEYPN 65

[Close]

BLAST of CF417240 vs. ExPASy Swiss-Prot
Match: UBC2_ASHGO (Ubiquitin-conjugating enzyme E2 2 OS=Ashbya gossypii GN=UBC2 PE=3 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 6.745e-23
Identity = 30/80 (37.50%), Postives = 52/80 (65.00%), Query Frame = -3

Query:  290 PMAAPKVRFLTKIYHPNIDKLGRICLDIXKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKE 529
            P   P V+FL++++HPN+   G ICLDI +++W+P   + ++L SIQ+L + PNP  P +   A  +K ++++ V+  KE
Sbjct:   64 PNKPPHVKFLSEMFHPNVYANGEICLDILQNRWTPTYDVASILTSIQSLFNDPNPASPANVEAATLFKDHKSQYVKRVKE 143

HSP 2 Score: 56.9954 bits (136), Expect = 6.745e-23
Identity = 25/52 (48.08%), Postives = 34/52 (65.38%), Query Frame = -2

Query:  525 QRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYXN 680
            +R+  +  PG+SASP  DN+  +N MI+GP  +PYE G F+L L   EEY N
Sbjct:   14 KRMKEDAPPGVSASPLPDNVMVWNAMIIGPADTPYEDGTFRLLLEFDEEYPN 65

[Close]

BLAST of CF417240 vs. ExPASy Swiss-Prot
Match: UBE2B_RAT (Ubiquitin-conjugating enzyme E2 B OS=Rattus norvegicus GN=Ube2b PE=2 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 6.780e-23
Identity = 32/73 (43.84%), Postives = 49/73 (67.12%), Query Frame = -3

Query:  311 PMAAPKVRFLTKIYHPNIDKLGRICLDIXKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAE 529
            P   P VRFL+K++HPN+   G ICLDI +++WSP   + ++L SIQ+LL  PNP+ P +   A+ ++ N+ E
Sbjct:   64 PNKPPTVRFLSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKRE 136

HSP 2 Score: 53.1434 bits (126), Expect = 6.780e-23
Identity = 23/52 (44.23%), Postives = 34/52 (65.38%), Query Frame = -2

Query:  525 QRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYXN 680
            +RL  +P  G+S +PSE+N+  +N +I GP  +P+E G FKL +   EEY N
Sbjct:   14 KRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPN 65

[Close]

BLAST of CF417240 vs. ExPASy Swiss-Prot
Match: UBE2B_RABIT (Ubiquitin-conjugating enzyme E2 B OS=Oryctolagus cuniculus GN=UBE2B PE=2 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 6.780e-23
Identity = 32/73 (43.84%), Postives = 49/73 (67.12%), Query Frame = -3

Query:  311 PMAAPKVRFLTKIYHPNIDKLGRICLDIXKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAE 529
            P   P VRFL+K++HPN+   G ICLDI +++WSP   + ++L SIQ+LL  PNP+ P +   A+ ++ N+ E
Sbjct:   64 PNKPPTVRFLSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKRE 136

HSP 2 Score: 53.1434 bits (126), Expect = 6.780e-23
Identity = 23/52 (44.23%), Postives = 34/52 (65.38%), Query Frame = -2

Query:  525 QRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYXN 680
            +RL  +P  G+S +PSE+N+  +N +I GP  +P+E G FKL +   EEY N
Sbjct:   14 KRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPN 65

[Close]

BLAST of CF417240 vs. ExPASy Swiss-Prot
Match: UBE2B_MOUSE (Ubiquitin-conjugating enzyme E2 B OS=Mus musculus GN=Ube2b PE=1 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 6.780e-23
Identity = 32/73 (43.84%), Postives = 49/73 (67.12%), Query Frame = -3

Query:  311 PMAAPKVRFLTKIYHPNIDKLGRICLDIXKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAE 529
            P   P VRFL+K++HPN+   G ICLDI +++WSP   + ++L SIQ+LL  PNP+ P +   A+ ++ N+ E
Sbjct:   64 PNKPPTVRFLSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKRE 136

HSP 2 Score: 53.1434 bits (126), Expect = 6.780e-23
Identity = 23/52 (44.23%), Postives = 34/52 (65.38%), Query Frame = -2

Query:  525 QRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYXN 680
            +RL  +P  G+S +PSE+N+  +N +I GP  +P+E G FKL +   EEY N
Sbjct:   14 KRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPN 65

The following BLAST results are available for this feature:

BLAST of CF417240 vs. ExPASy Swiss-Prot

BLAST of CF417240 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 199


Match Name	E-value	Identity	Description
UBE2K_MOUSE	1.061e-23	52.87	Ubiquitin-conjugating enzyme E2 K OS=Mus musculus ...	[more]
UBE2K_HUMAN	1.061e-23	52.87	Ubiquitin-conjugating enzyme E2 K OS=Homo sapiens ...	[more]
UBE2K_BOVIN	1.061e-23	52.87	Ubiquitin-conjugating enzyme E2 K OS=Bos taurus GN...	[more]
UBC1_YEAST	3.952e-23	47.73	Ubiquitin-conjugating enzyme E2-24 kDa OS=Saccharo...	[more]
UBCD4_DROME	3.960e-23	52.81	Ubiquitin-conjugating enzyme E2-22 kDa OS=Drosophi...	[more]
UBC2_YEAST	6.740e-23	37.50	Ubiquitin-conjugating enzyme E2 2 OS=Saccharomyces...	[more]
UBC2_ASHGO	6.745e-23	37.50	Ubiquitin-conjugating enzyme E2 2 OS=Ashbya gossyp...	[more]
UBE2B_RAT	6.780e-23	43.84	Ubiquitin-conjugating enzyme E2 B OS=Rattus norveg...	[more]
UBE2B_RABIT	6.780e-23	43.84	Ubiquitin-conjugating enzyme E2 B OS=Oryctolagus c...	[more]
UBE2B_MOUSE	6.780e-23	43.84	Ubiquitin-conjugating enzyme E2 B OS=Mus musculus ...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_115000-170 Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange Citrus sinensis cDNA clone IDFL-003_A07 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF417240 ID=CF417240; Name=CF417240; organism=Citrus sinensis; type=EST; length=681bp
GTTTTTTTTTTTTTTTTTTTTTGCTTCGAAATTTAAGAGTTGAGAAACAA
TGTATATCATCTAATCATACAAGTTCAAAGACATGGCTTCCACAAGAAGA
AAATAATTGACATGTGCGGTAAGATGGAAGCTTTCAGAATGTCAAACTCC
GAATGTTCAAACATCAATTCAATTCTTCCAAATTTTCCATTTTAAACACA
GTGAATAGCATACATTGCACAATTGTTATTTGAATGAAGTGATATTTTCC
ACCATGTCATCAAGCACCACTGGCATATAAACGTGTCCATTCCTTCGCTG
TTTCAACCGCTTCGGCCTCATTTGTTTTCCAGTGTTTGGCAATGTTCTCA
GAGAGAGGATCATCGGGGTTAGGAGCACTCAGAAGTGCTTGAATGCTCAA
AAGTACAGTCCGAATCTGGAGAGCTGGACTCCACTTGTCTTTCANAATAT
CAAGACAAATCCTTCCAAGCTTATCAATGTTAGGATGATATATCTTGGTA
AGAAATCGGACCTTTGGAGCAGCCATTGGNATATTCTTCAGGCAAAAATA
ATTCCAATTTGAACACCCCTCCTTCATATGGAGATTGTGTTGGACCAAGG
ATCATCACATTAAAGTATCGCATATTATCTTCCGAGGGAGATGCACTTAT
TCCTGGTGCTGGTTCACTGAGGAGACGCTGT