CF417240 | Citrus Genome Database

Overview


Name	CF417240
Unique Name	CF417240
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	681

Libraries


Library Name	Type
Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig4591	contig	Csv1_Contig4591:178..859.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CF417240 vs. ExPASy Swiss-Prot
Match: UBC1_SCHPO (Ubiquitin-conjugating enzyme E2 1 OS=Schizosaccharomyces pombe GN=ubc1 PE=2 SV=2)

HSP 1 Score: 87.8113 bits (216), Expect = 5.866e-21
Identity = 40/88 (45.45%), Postives = 57/88 (64.77%), Query Frame = -3

Query:  269 PMAAPKVRFLTKIYHPNID-KLGRICLDIXKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKEWTRLYAS 529
            P   PK+ F TKIYHPN+  + G ICLDI KD+WSP   +++ L+S+Q+LL  P P +P    +A+ +  N  + V TA+EWT  YA+
Sbjct:   64 PFRPPKMNFDTKIYHPNVSSQTGAICLDILKDQWSPVYTMKSALISLQSLLCTPEPSNPQDAQVAQVYLQNYQQFVRTAREWTSSYAA 151

HSP 2 Score: 33.4982 bits (75), Expect = 5.866e-21
Identity = 14/41 (34.15%), Postives = 23/41 (56.10%), Query Frame = -2

Query:  531 GISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEY 653
            GI      D++ +   M  GP  +PYEGG F +++ +P +Y
Sbjct:   23 GIQVWTINDDISHLKGMFRGPEGTPYEGGYFVVDIEIPIDY 63

[Close]

BLAST of CF417240 vs. ExPASy Swiss-Prot
Match: UBC2_EMENI (Ubiquitin-conjugating enzyme E2 2 OS=Emericella nidulans GN=uvsJ PE=3 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 5.952e-21
Identity = 27/80 (33.75%), Postives = 49/80 (61.25%), Query Frame = -3

Query:  290 PMAAPKVRFLTKIYHPNIDKLGRICLDIXKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKE 529
            P   P V+F+++++HPN+   G +CLDI +++WSP   +  +L SIQ+LL+ PN   P +   +  ++ N  E ++  +E
Sbjct:   64 PNKPPGVKFISQMFHPNVYGTGELCLDILQNRWSPTYDVAAILTSIQSLLNDPNTSSPANVEASNLYRDNRKEYIKRVRE 143

HSP 2 Score: 53.1434 bits (126), Expect = 5.952e-21
Identity = 21/52 (40.38%), Postives = 35/52 (67.31%), Query Frame = -2

Query:  525 QRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYXN 680
            +R+ ++P  G+SASP  DN+  +N +I+GP  +P+E G F+L +   E+Y N
Sbjct:   14 KRMQTDPPAGVSASPVADNVMTWNAVIIGPADTPFEDGTFRLVMHFEEQYPN 65

[Close]

BLAST of CF417240 vs. ExPASy Swiss-Prot
Match: COP10_ARATH (Constitutive photomorphogenesis protein 10 OS=Arabidopsis thaliana GN=COP10 PE=1 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 7.679e-21
Identity = 35/86 (40.70%), Postives = 51/86 (59.30%), Query Frame = -3

Query:  272 PMAAPKVRFLTKIYHPNIDKLGRICLDIXKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKEWTRLYA 529
            P   PK+ F T+IYH N+D  G + ++I +D WSPAL I  VL +I+++   P P  P    IA+ + T+  +  E AKEWT  +A
Sbjct:   96 PFKPPKLVFKTRIYHCNVDTAGDLSVNILRDSWSPALTITKVLQAIRSIFLKPEPYSPALPVIARLYLTDREKHDEVAKEWTLRFA 181

HSP 2 Score: 49.6766 bits (117), Expect = 7.679e-21
Identity = 19/45 (42.22%), Postives = 30/45 (66.67%), Query Frame = -2

Query:  531 EPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEY 665
            +P P  SA P  DN+ ++   I+GP+ +PYEGG+F L++  P +Y
Sbjct:   51 DPPPDCSAGPKGDNLYHWIATIIGPSGTPYEGGIFFLDIIFPSDY 95

[Close]

BLAST of CF417240 vs. ExPASy Swiss-Prot
Match: UBC2_CANAL (Ubiquitin-conjugating enzyme E2 2 OS=Candida albicans GN=UBC2 PE=2 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 1.300e-20
Identity = 27/80 (33.75%), Postives = 51/80 (63.75%), Query Frame = -3

Query:  290 PMAAPKVRFLTKIYHPNIDKLGRICLDIXKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKE 529
            P   P+V+F+++++HPN+   G +CLDI +++WSP   + ++L S+Q+LL+ PN   P +   A  +K + +  V+  +E
Sbjct:   64 PNKPPQVKFISEMFHPNVYASGELCLDILQNRWSPTYDVSSILTSVQSLLNDPNISSPANVEAANLYKDHRSLYVKRVRE 143

HSP 2 Score: 53.1434 bits (126), Expect = 1.300e-20
Identity = 22/52 (42.31%), Postives = 35/52 (67.31%), Query Frame = -2

Query:  525 QRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYXN 680
            +R+  +P  G+SASP  DN+  +N +I+GP+ +P+E G F+L L   E+Y N
Sbjct:   14 KRMQQDPPSGVSASPLPDNVMKWNAVIIGPSDTPFEDGTFRLLLSFDEQYPN 65

[Close]

BLAST of CF417240 vs. ExPASy Swiss-Prot
Match: UBCB_DICDI (Ubiquitin conjugating enzyme E2 B OS=Dictyostelium discoideum GN=ubcB PE=1 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 1.312e-20
Identity = 41/87 (47.13%), Postives = 57/87 (65.52%), Query Frame = -3

Query:  272 PMAAPKVRFLTKIYHPNIDKL-GRICLDIXKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKEWTRLYA 529
            P   P ++F TKIYHPNI    G IC ++    WSP L+I  VL +I+++L+ PNPD+PL   IA+ +KT+     +TAKEWT+ YA
Sbjct:   62 PFKPPTLKFTTKIYHPNIKTSDGAICAEVFST-WSPQLKILDVLTTIRSILTDPNPDNPLETEIAQQFKTDRNAFNKTAKEWTKKYA 147

HSP 2 Score: 38.5058 bits (88), Expect = 1.312e-20
Identity = 16/48 (33.33%), Postives = 28/48 (58.33%), Query Frame = -2

Query:  531 LLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEY 674
            +L  P    SA   +DN++ +   + GP  SP+E GVF +++ +P +Y
Sbjct:   14 MLKTPPSWCSAHLVDDNLQKWKATVQGPEGSPFEKGVFSMDIDIPADY 61

[Close]

BLAST of CF417240 vs. ExPASy Swiss-Prot
Match: UBC2_SCHPO (Ubiquitin-conjugating enzyme E2 2 OS=Schizosaccharomyces pombe GN=rhp6 PE=1 SV=3)

HSP 1 Score: 64.6994 bits (156), Expect = 1.705e-20
Identity = 28/80 (35.00%), Postives = 48/80 (60.00%), Query Frame = -3

Query:  290 PMAAPKVRFLTKIYHPNIDKLGRICLDIXKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKE 529
            P   P V+F++ ++HPN+   G +CLDI +++WSP   +  +L SIQ+LL+ PN   P +   A+  + N+ E V   ++
Sbjct:   64 PNKPPLVKFVSTMFHPNVYANGELCLDILQNRWSPTYDVAAILTSIQSLLNDPNNASPANAEAAQLHRENKKEYVRRVRK 143

HSP 2 Score: 55.0694 bits (131), Expect = 1.705e-20
Identity = 23/52 (44.23%), Postives = 34/52 (65.38%), Query Frame = -2

Query:  525 QRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYXN 680
            +R+  +P  G+SASP  DN+  +N +I+GP  +P+E G FKL L   E+Y N
Sbjct:   14 KRMQQDPPAGVSASPVSDNVMLWNAVIIGPADTPFEDGTFKLVLSFDEQYPN 65

[Close]

BLAST of CF417240 vs. ExPASy Swiss-Prot
Match: UBC2_DEBHA (Ubiquitin-conjugating enzyme E2 2 OS=Debaryomyces hansenii GN=UBC2 PE=3 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 6.314e-20
Identity = 27/80 (33.75%), Postives = 51/80 (63.75%), Query Frame = -3

Query:  290 PMAAPKVRFLTKIYHPNIDKLGRICLDIXKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKE 529
            P   P V+F+++++HPN+   G +CLDI +++WSP   + ++L SIQ+LL+ PN   P +   A  +K + ++ ++  +E
Sbjct:   64 PNKPPSVKFISEMFHPNVYASGELCLDILQNRWSPTYDVSSILTSIQSLLNDPNISSPANVEAANLYKDHRSQYIKRVRE 143

HSP 2 Score: 49.2914 bits (116), Expect = 6.314e-20
Identity = 21/52 (40.38%), Postives = 33/52 (63.46%), Query Frame = -2

Query:  525 QRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYXN 680
            +R+  +   G+SASP  DN+  +N +I+GP  +P+E G F+L L   E+Y N
Sbjct:   14 KRMQQDAPSGVSASPLPDNVMSWNAVIIGPADTPFEDGTFRLILQFDEQYPN 65

[Close]

BLAST of CF417240 vs. ExPASy Swiss-Prot
Match: UBC11_YEAST (Ubiquitin-conjugating enzyme E2-18 kDa OS=Saccharomyces cerevisiae GN=UBC11 PE=1 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 6.337e-20
Identity = 34/76 (44.74%), Postives = 49/76 (64.47%), Query Frame = -3

Query:  311 KNXPMAAPKVRFLTKIYHPNIDKLGRICLDIXKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAE 538
            +N P   P ++FL+ ++HPN+DK G ICLDI K+KWS    + T+LLS+Q+LL  PN   PL+   A+ W  +  E
Sbjct:   66 QNYPFHPPMIKFLSPMWHPNVDKSGNICLDILKEKWSAVYNVETILLSLQSLLGEPNNRSPLNAVAAELWDADMEE 141

HSP 2 Score: 38.891 bits (89), Expect = 6.337e-20
Identity = 21/50 (42.00%), Postives = 28/50 (56.00%), Query Frame = -2

Query:  531 RLLSEPAPGISASPSEDN-MRYFNVMILGPTQSPYEGGVFKLELFLPEEY 677
            +LLS     ISA P +DN + Y+   I GP  +PY G  FK+ L  P+ Y
Sbjct:   19 QLLSSTTESISAFPVDDNDLTYWVGYITGPKDTPYSGLKFKVSLKFPQNY 68

[Close]

BLAST of CF417240 vs. ExPASy Swiss-Prot
Match: UBC2_NEUCR (Ubiquitin-conjugating enzyme E2 2 OS=Neurospora crassa GN=mus-8 PE=3 SV=3)

HSP 1 Score: 64.6994 bits (156), Expect = 6.351e-20
Identity = 28/80 (35.00%), Postives = 47/80 (58.75%), Query Frame = -3

Query:  290 PMAAPKVRFLTKIYHPNIDKLGRICLDIXKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKE 529
            P   P V+F+++++HPN+   G +CLDI +++WSP   +  VL SIQ+LL+ PN     +   +  +K N  E  +  +E
Sbjct:   64 PNKPPSVKFISEMFHPNVYATGELCLDILQNRWSPTYDVAAVLTSIQSLLNDPNTGSRANVEASNLYKDNRKEYHKRVRE 143

HSP 2 Score: 53.1434 bits (126), Expect = 6.351e-20
Identity = 21/52 (40.38%), Postives = 35/52 (67.31%), Query Frame = -2

Query:  525 QRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYXN 680
            +R+ ++P  G+SASP  DN+  +N +I+GP  +P+E G F+L +   E+Y N
Sbjct:   14 KRMQTDPPAGVSASPVPDNVMTWNAVIIGPADTPFEDGTFRLVMHFEEQYPN 65

[Close]

BLAST of CF417240 vs. ExPASy Swiss-Prot
Match: UBC21_CAEEL (Probable ubiquitin-conjugating enzyme E2 21 OS=Caenorhabditis elegans GN=ubc-21 PE=2 SV=2)

HSP 1 Score: 81.2629 bits (199), Expect = 1.374e-19
Identity = 39/83 (46.99%), Postives = 55/83 (66.27%), Query Frame = -3

Query:  269 KVRFLTKIYHPNID-KLGRICLDIXKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKEWTRLYAS 514
            + +F+T+I+HPNI  + G ICLDI KDKW+ +L +RTVLLS+QA+L +P P DP    +AK +  N      TA  WT  +A+
Sbjct:  101 QAKFVTRIWHPNISSQTGTICLDILKDKWTASLTLRTVLLSLQAMLCSPEPSDPQDAVVAKQFINNYPMFTATAVYWTSYFAN 183

HSP 2 Score: 35.4242 bits (80), Expect = 1.374e-19
Identity = 15/41 (36.59%), Postives = 23/41 (56.10%), Query Frame = -2

Query:  531 GISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEY 653
            GI     E+N+      I GP  +PY GG F++++ +PE Y
Sbjct:   40 GIHVEIKENNLMDIKGFIKGPEGTPYAGGTFEIKVDIPEHY 80

The following BLAST results are available for this feature:

BLAST of CF417240 vs. ExPASy Swiss-Prot

BLAST of CF417240 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 199


Match Name	E-value	Identity	Description
UBC1_SCHPO	5.866e-21	45.45	Ubiquitin-conjugating enzyme E2 1 OS=Schizosacchar...	[more]
UBC2_EMENI	5.952e-21	33.75	Ubiquitin-conjugating enzyme E2 2 OS=Emericella ni...	[more]
COP10_ARATH	7.679e-21	40.70	Constitutive photomorphogenesis protein 10 OS=Arab...	[more]
UBC2_CANAL	1.300e-20	33.75	Ubiquitin-conjugating enzyme E2 2 OS=Candida albic...	[more]
UBCB_DICDI	1.312e-20	47.13	Ubiquitin conjugating enzyme E2 B OS=Dictyostelium...	[more]
UBC2_SCHPO	1.705e-20	35.00	Ubiquitin-conjugating enzyme E2 2 OS=Schizosacchar...	[more]
UBC2_DEBHA	6.314e-20	33.75	Ubiquitin-conjugating enzyme E2 2 OS=Debaryomyces ...	[more]
UBC11_YEAST	6.337e-20	44.74	Ubiquitin-conjugating enzyme E2-18 kDa OS=Saccharo...	[more]
UBC2_NEUCR	6.351e-20	35.00	Ubiquitin-conjugating enzyme E2 2 OS=Neurospora cr...	[more]
UBC21_CAEEL	1.374e-19	46.99	Probable ubiquitin-conjugating enzyme E2 21 OS=Cae...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_115000-170 Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange Citrus sinensis cDNA clone IDFL-003_A07 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF417240 ID=CF417240; Name=CF417240; organism=Citrus sinensis; type=EST; length=681bp
GTTTTTTTTTTTTTTTTTTTTTGCTTCGAAATTTAAGAGTTGAGAAACAA
TGTATATCATCTAATCATACAAGTTCAAAGACATGGCTTCCACAAGAAGA
AAATAATTGACATGTGCGGTAAGATGGAAGCTTTCAGAATGTCAAACTCC
GAATGTTCAAACATCAATTCAATTCTTCCAAATTTTCCATTTTAAACACA
GTGAATAGCATACATTGCACAATTGTTATTTGAATGAAGTGATATTTTCC
ACCATGTCATCAAGCACCACTGGCATATAAACGTGTCCATTCCTTCGCTG
TTTCAACCGCTTCGGCCTCATTTGTTTTCCAGTGTTTGGCAATGTTCTCA
GAGAGAGGATCATCGGGGTTAGGAGCACTCAGAAGTGCTTGAATGCTCAA
AAGTACAGTCCGAATCTGGAGAGCTGGACTCCACTTGTCTTTCANAATAT
CAAGACAAATCCTTCCAAGCTTATCAATGTTAGGATGATATATCTTGGTA
AGAAATCGGACCTTTGGAGCAGCCATTGGNATATTCTTCAGGCAAAAATA
ATTCCAATTTGAACACCCCTCCTTCATATGGAGATTGTGTTGGACCAAGG
ATCATCACATTAAAGTATCGCATATTATCTTCCGAGGGAGATGCACTTAT
TCCTGGTGCTGGTTCACTGAGGAGACGCTGT