CF417270 | Citrus Genome Database

Overview


Name	CF417270
Unique Name	CF417270
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	691

Libraries


Library Name	Type
Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CF417270 vs. ExPASy Swiss-Prot
Match: UBC9_MESAU (SUMO-conjugating enzyme UBC9 OS=Mesocricetus auratus GN=UBE2I PE=2 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 2.818e-42
Identity = 46/75 (61.33%), Postives = 54/75 (72.00%), Query Frame = 2

Query:  215 GIARGRLTEERKAWRKNHPHGFVAKP-ETKDGSVNLMIWECIIPGKTGTDWEGGYFPLTLYFSEDYPSNPQSVSF 436
            GIA  RL +ERKAWRK+HP GFVA P +  DG++NLM WEC IPGK GT WEGG F L + F +DYPS+P    F
Sbjct:    3 GIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKF 77

HSP 2 Score: 84.7297 bits (208), Expect = 2.818e-42
Identity = 36/49 (73.47%), Postives = 40/49 (81.63%), Query Frame = 1

Query:  418 PPKCKFPQGFFHPNVYPSGTVCLSILNEDNGWRPAITVKQILVGIQDLL 564
            PPKCKF    FHPNVYPSGTVCLSIL ED  WRPAIT+ Q+ +GIQ+LL
Sbjct:   72 PPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITINQLFIGIQELL 120

HSP 3 Score: 27.335 bits (59), Expect = 2.818e-42
Identity = 10/24 (41.67%), Postives = 17/24 (70.83%), Query Frame = 3

Query:  606 YQLFIQDPAEYKRRVRQQAKQYPP 677
            Y ++ Q+  EY++R R QAK++ P
Sbjct:  134 YTIYCQNRVEYEKRFRAQAKKFCP 157

[Close]

BLAST of CF417270 vs. ExPASy Swiss-Prot
Match: UBC9_CAEEL (SUMO-conjugating enzyme UBC9 OS=Caenorhabditis elegans GN=ubc-9 PE=1 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 2.439e-40
Identity = 41/75 (54.67%), Postives = 52/75 (69.33%), Query Frame = 2

Query:  215 GIARGRLTEERKAWRKNHPHGFVAKP-ETKDGSVNLMIWECIIPGKTGTDWEGGYFPLTLYFSEDYPSNPQSVSF 436
            GIA GRL EERK WRK+HP GF+AKP +  DG++NL  WEC IPG+  T WEGG + + + F +D+PS P    F
Sbjct:    3 GIAAGRLAEERKHWRKDHPFGFIAKPVKNADGTLNLFNWECAIPGRKDTIWEGGLYRIRMLFKDDFPSTPPKCKF 77

HSP 2 Score: 83.5741 bits (205), Expect = 2.439e-40
Identity = 34/49 (69.39%), Postives = 43/49 (87.76%), Query Frame = 1

Query:  418 PPKCKFPQGFFHPNVYPSGTVCLSILNEDNGWRPAITVKQILVGIQDLL 564
            PPKCKF    FHPNVYPSGTVCLS+L+E+  W+P+I++KQ+L+GIQDLL
Sbjct:   72 PPKCKFEPPLFHPNVYPSGTVCLSLLDENKDWKPSISIKQLLIGIQDLL 120

HSP 3 Score: 28.8758 bits (63), Expect = 2.439e-40
Identity = 10/22 (45.45%), Postives = 19/22 (86.36%), Query Frame = 3

Query:  606 YQLFIQDPAEYKRRVRQQAKQY 671
            YQ++ Q+ AEY++RV+++A +Y
Sbjct:  134 YQIYCQNRAEYEKRVKKEAVKY 155

[Close]

BLAST of CF417270 vs. ExPASy Swiss-Prot
Match: UBC9_DICDI (Sumo-conjugating enzyme ubc9 OS=Dictyostelium discoideum GN=ubc9 PE=3 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 3.263e-24
Identity = 57/129 (44.19%), Postives = 73/129 (56.59%), Query Frame = 2

Query:  215 GIARGRLTEERKAWRKNHPHGFVAKPETK-DGSVNLMIWECIIPGKTGTDWEGGYFPLTLYFSEDYPSNPQSVSFLKVSFILMSTLLEQYACQSSMRTMGGDLPLL*SK-F*WAFRICWDQPNPADPAQ 595
            GI+  RL+EERK WR++HP+GF A+P T  DGS+NL +W C IPGKT T+WEGG +PL + F+EDYPS P    F K  F   +       C S +       P +  K      +   D PNP  PAQ
Sbjct:    3 GISSARLSEERKNWRRDHPYGFFARPSTNTDGSLNLYVWNCGIPGKTKTNWEGGVYPLIMEFTEDYPSKPPKCRFPK-DFFHPNVYPSGTVCLSILNEEADWKPSVTIKTVLLGIQDLLDNPNPKSPAQ 130

HSP 2 Score: 27.7202 bits (60), Expect = 3.263e-24
Identity = 10/22 (45.45%), Postives = 16/22 (72.73%), Query Frame = 3

Query:  612 LFIQDPAEYKRRVRQQAKQYPP 677
            LF+ +  EY ++V+ Q+K YPP
Sbjct:  136 LFLTNKEEYDKKVKAQSKVYPP 157

[Close]

BLAST of CF417270 vs. ExPASy Swiss-Prot
Match: UBC9_SCHPO (SUMO-conjugating enzyme ubc9 OS=Schizosaccharomyces pombe GN=hus5 PE=1 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 2.518e-20
Identity = 39/49 (79.59%), Postives = 44/49 (89.80%), Query Frame = 1

Query:  418 PPKCKFPQGFFHPNVYPSGTVCLSILNEDNGWRPAITVKQILVGIQDLL 564
            PPKC+F    FHPNVYPSGTVCLSILNE+ GW+PAIT+KQIL+GIQDLL
Sbjct:   72 PPKCRFTPPLFHPNVYPSGTVCLSILNEEEGWKPAITIKQILLGIQDLL 120

HSP 2 Score: 28.4906 bits (62), Expect = 2.518e-20
Identity = 11/24 (45.83%), Postives = 17/24 (70.83%), Query Frame = 3

Query:  606 YQLFIQDPAEYKRRVRQQAKQYPP 677
            Y +F +D  EY++RVR QA++  P
Sbjct:  134 YTMFKKDKVEYEKRVRAQARENAP 157

HSP 3 Score: 88.1965 bits (217), Expect = 4.508e-17
Identity = 52/135 (38.52%), Postives = 70/135 (51.85%), Query Frame = 2

Query:  218 IARGRLTEERKAWRKNHPHGFVAKP-ETKDGSVNLMIWECIIPGKTGTDWEGGYFPLTLYFSEDYPSNPQSVSFLKVSFILMSTLLEQYACQSSMRTMGGDLPLL*SK-F*WAFRICWDQPNPADPAQTDGIPAF 616
            + + RL EERK WR++HP GF AKP ++ DG ++LM W+  IPGK  T WEGG + LT+ F E+YP+ P    F    F   +       C S +    G  P +  K      +   D PN A PAQT+    F
Sbjct:    4 LCKTRLQEERKQWRRDHPFGFYAKPCKSSDGGLDLMNWKVGIPGKPKTSWEGGLYKLTMAFPEEYPTRPPKCRFTPPLF-HPNVYPSGTVCLSILNEEEGWKPAITIKQILLGIQDLLDDPNIASPAQTEAYTMF 137

[Close]

BLAST of CF417270 vs. ExPASy Swiss-Prot
Match: UBC9_YEAST (SUMO-conjugating enzyme UBC9 OS=Saccharomyces cerevisiae GN=UBC9 PE=1 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 5.541e-20
Identity = 40/51 (78.43%), Postives = 46/51 (90.20%), Query Frame = 1

Query:  418 PPKCKFPQGFFHPNVYPSGTVCLSILNEDNGWRPAITVKQILVGIQDLLGS 570
            PPK KFP GF+HPNVYPSGT+CLSILNED  WRPAIT+KQI++G+QDLL S
Sbjct:   72 PPKVKFPAGFYHPNVYPSGTICLSILNEDQDWRPAITLKQIVLGVQDLLDS 122

HSP 2 Score: 24.2534 bits (51), Expect = 5.541e-20
Identity = 10/22 (45.45%), Postives = 16/22 (72.73%), Query Frame = 3

Query:  606 YQLFIQDPAEYKRRVRQQAKQY 671
            ++ F ++ AEY ++V  QAKQY
Sbjct:  134 WRSFSRNKAEYDKKVLLQAKQY 155

HSP 3 Score: 89.7373 bits (221), Expect = 1.549e-17
Identity = 54/141 (38.30%), Postives = 69/141 (48.94%), Query Frame = 2

Query:  230 RLTEERKAWRKNHPHGFVAKPETK-DGSVNLMIWECIIPGKTGTDWEGGYFPLTLYFSEDYPSNPQSVSFLKVSFILMSTLLEQYACQSSMRTMGGDLPLL*SK-F*WAFRICWDQPNPADPAQTDGIPAFHPGSCRVQEK 646
            RL EERK WRK+HP GF AKP  K DGS++L  WE  IPGK GT+W GG +P+T+ +  +YPS P  V F    F   +       C S +       P +  K      +   D PNP  PAQ     +F        +K
Sbjct:    8 RLQEERKKWRKDHPFGFYAKPVKKADGSMDLQKWEAGIPGKEGTNWAGGVYPITVEYPNEYPSKPPKVKF-PAGFYHPNVYPSGTICLSILNEDQDWRPAITLKQIVLGVQDLLDSPNPNSPAQEPAWRSFSRNKAEYDKK 147

[Close]

BLAST of CF417270 vs. ExPASy Swiss-Prot
Match: UBC7_YEAST (Ubiquitin-conjugating enzyme E2-18 kDa OS=Saccharomyces cerevisiae GN=UBC7 PE=1 SV=1)

HSP 1 Score: 51.9878 bits (123), Expect = 2.771e-14
Identity = 26/72 (36.11%), Postives = 42/72 (58.33%), Query Frame = 2

Query:  221 ARGRLTEERKAWRKNHPHGFVAKPETKDGSVNLMIWECIIPGKTGTDWEGGYFPLTLYFSEDYPSNPQSVSF 436
            A+ RL +E +   K+ P G VA P++++   N+ IW+C+I G   T +  G F   L F +DYP +P  ++F
Sbjct:    5 AQKRLLKELQQLIKDSPPGIVAGPKSEN---NIFIWDCLIQGPPDTPYADGVFNAKLEFPKDYPLSPPKLTF 73

HSP 2 Score: 46.9802 bits (110), Expect = 2.771e-14
Identity = 21/60 (35.00%), Postives = 32/60 (53.33%), Query Frame = 1

Query:  418 PPKCKFPQGFFHPNVYPSGTVCLSILNE-----------DNGWRPAITVKQILVGIQDLL 564
            PPK  F     HPN+YP+G VC+SIL+            +  W P  +V++IL+ +  +L
Sbjct:   68 PPKLTFTPSILHPNIYPNGEVCISILHSPGDDPNMYELAEERWSPVQSVEKILLSVMSML 127

[Close]

BLAST of CF417270 vs. ExPASy Swiss-Prot
Match: UB2G2_PONAB (Ubiquitin-conjugating enzyme E2 G2 OS=Pongo abelii GN=UBE2G2 PE=2 SV=1)

HSP 1 Score: 48.1358 bits (113), Expect = 4.934e-13
Identity = 29/74 (39.19%), Postives = 35/74 (47.30%), Query Frame = 2

Query:  215 GIARGRLTEERKAWRKNHPHGFVAKPETKDGSVNLMIWECIIPGKTGTDWEGGYFPLTLYFSEDYPSNPQSVSF 436
            G A  RL  E K    N P G VA P  ++   N   WE +I G   T +E G FP  L F  DYP +P  + F
Sbjct:    3 GTALKRLMAEYKQLTLNPPEGIVAGPMNEE---NFFEWEALIMGPEDTCFEFGVFPAILSFPLDYPLSPPKMRF 73

HSP 2 Score: 46.595 bits (109), Expect = 4.934e-13
Identity = 22/60 (36.67%), Postives = 32/60 (53.33%), Query Frame = 1

Query:  418 PPKCKFPQGFFHPNVYPSGTVCLSILNEDNG-----------WRPAITVKQILVGIQDLL 564
            PPK +F    FHPN+YP G VC+SIL+               W P  +V++IL+ +  +L
Sbjct:   68 PPKMRFTCEMFHPNIYPDGRVCISILHAPGDDPMGYESSAERWSPVQSVEKILLSVVSML 127

[Close]

BLAST of CF417270 vs. ExPASy Swiss-Prot
Match: UB2G2_MOUSE (Ubiquitin-conjugating enzyme E2 G2 OS=Mus musculus GN=Ube2g2 PE=1 SV=1)

HSP 1 Score: 48.1358 bits (113), Expect = 4.934e-13
Identity = 29/74 (39.19%), Postives = 35/74 (47.30%), Query Frame = 2

Query:  215 GIARGRLTEERKAWRKNHPHGFVAKPETKDGSVNLMIWECIIPGKTGTDWEGGYFPLTLYFSEDYPSNPQSVSF 436
            G A  RL  E K    N P G VA P  ++   N   WE +I G   T +E G FP  L F  DYP +P  + F
Sbjct:    3 GTALKRLMAEYKQLTLNPPEGIVAGPMNEE---NFFEWEALIMGPEDTCFEFGVFPAILSFPLDYPLSPPKMRF 73

HSP 2 Score: 46.595 bits (109), Expect = 4.934e-13
Identity = 22/60 (36.67%), Postives = 32/60 (53.33%), Query Frame = 1

Query:  418 PPKCKFPQGFFHPNVYPSGTVCLSILNEDNG-----------WRPAITVKQILVGIQDLL 564
            PPK +F    FHPN+YP G VC+SIL+               W P  +V++IL+ +  +L
Sbjct:   68 PPKMRFTCEMFHPNIYPDGRVCISILHAPGDDPMGYESSAERWSPVQSVEKILLSVVSML 127

[Close]

BLAST of CF417270 vs. ExPASy Swiss-Prot
Match: UB2G2_HUMAN (Ubiquitin-conjugating enzyme E2 G2 OS=Homo sapiens GN=UBE2G2 PE=1 SV=1)

HSP 1 Score: 48.1358 bits (113), Expect = 4.934e-13
Identity = 29/74 (39.19%), Postives = 35/74 (47.30%), Query Frame = 2

Query:  215 GIARGRLTEERKAWRKNHPHGFVAKPETKDGSVNLMIWECIIPGKTGTDWEGGYFPLTLYFSEDYPSNPQSVSF 436
            G A  RL  E K    N P G VA P  ++   N   WE +I G   T +E G FP  L F  DYP +P  + F
Sbjct:    3 GTALKRLMAEYKQLTLNPPEGIVAGPMNEE---NFFEWEALIMGPEDTCFEFGVFPAILSFPLDYPLSPPKMRF 73

HSP 2 Score: 46.595 bits (109), Expect = 4.934e-13
Identity = 22/60 (36.67%), Postives = 32/60 (53.33%), Query Frame = 1

Query:  418 PPKCKFPQGFFHPNVYPSGTVCLSILNEDNG-----------WRPAITVKQILVGIQDLL 564
            PPK +F    FHPN+YP G VC+SIL+               W P  +V++IL+ +  +L
Sbjct:   68 PPKMRFTCEMFHPNIYPDGRVCISILHAPGDDPMGYESSAERWSPVQSVEKILLSVVSML 127

[Close]

BLAST of CF417270 vs. ExPASy Swiss-Prot
Match: UB2G2_BOVIN (Ubiquitin-conjugating enzyme E2 G2 OS=Bos taurus GN=UBE2G2 PE=2 SV=1)

HSP 1 Score: 48.1358 bits (113), Expect = 4.934e-13
Identity = 29/74 (39.19%), Postives = 35/74 (47.30%), Query Frame = 2

Query:  215 GIARGRLTEERKAWRKNHPHGFVAKPETKDGSVNLMIWECIIPGKTGTDWEGGYFPLTLYFSEDYPSNPQSVSF 436
            G A  RL  E K    N P G VA P  ++   N   WE +I G   T +E G FP  L F  DYP +P  + F
Sbjct:    3 GTALKRLMAEYKQLTLNPPEGIVAGPMNEE---NFFEWEALIMGPEDTCFEFGVFPAILSFPLDYPLSPPKMRF 73

HSP 2 Score: 46.595 bits (109), Expect = 4.934e-13
Identity = 22/60 (36.67%), Postives = 32/60 (53.33%), Query Frame = 1

Query:  418 PPKCKFPQGFFHPNVYPSGTVCLSILNEDNG-----------WRPAITVKQILVGIQDLL 564
            PPK +F    FHPN+YP G VC+SIL+               W P  +V++IL+ +  +L
Sbjct:   68 PPKMRFTCEMFHPNIYPDGRVCISILHAPGDDPMGYESSAERWSPVQSVEKILLSVVSML 127

The following BLAST results are available for this feature:

BLAST of CF417270 vs. ExPASy Swiss-Prot

BLAST of CF417270 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 29


Match Name	E-value	Identity	Description
UBC9_MESAU	2.818e-42	61.33	SUMO-conjugating enzyme UBC9 OS=Mesocricetus aurat...	[more]
UBC9_CAEEL	2.439e-40	54.67	SUMO-conjugating enzyme UBC9 OS=Caenorhabditis ele...	[more]
UBC9_DICDI	3.263e-24	44.19	Sumo-conjugating enzyme ubc9 OS=Dictyostelium disc...	[more]
UBC9_SCHPO	2.518e-20	79.59	SUMO-conjugating enzyme ubc9 OS=Schizosaccharomyce...	[more]
UBC9_YEAST	5.541e-20	78.43	SUMO-conjugating enzyme UBC9 OS=Saccharomyces cere...	[more]
UBC7_YEAST	2.771e-14	36.11	Ubiquitin-conjugating enzyme E2-18 kDa OS=Saccharo...	[more]
UB2G2_PONAB	4.934e-13	39.19	Ubiquitin-conjugating enzyme E2 G2 OS=Pongo abelii...	[more]
UB2G2_MOUSE	4.934e-13	39.19	Ubiquitin-conjugating enzyme E2 G2 OS=Mus musculus...	[more]
UB2G2_HUMAN	4.934e-13	39.19	Ubiquitin-conjugating enzyme E2 G2 OS=Homo sapiens...	[more]
UB2G2_BOVIN	4.934e-13	39.19	Ubiquitin-conjugating enzyme E2 G2 OS=Bos taurus G...	[more]

Pages

Properties


Property Name	Value
Genbank description	USDA-FP_115000-200 Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange Citrus sinensis cDNA clone IDFL-003_D04 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF417270 ID=CF417270; Name=CF417270; organism=Citrus sinensis; type=EST; length=691bp
GAAATATAGAAGCATGGAAGTTGCGTAAGCTTCAATCTTCCGTTTAAGAA
TATCAGAAGTCAACATCCCGGGCAGCAAGCACGTACACGGTACTCGGTAC
TCGGTACTCGGTACTCGGTACTCATAAGCTTGTTTCTTCTGCAAACAAAC
ATTGATTAATACAGTTAGTTATACAAGCGATCATAATTCAAACGTAAGAG
TCATCATGTCGGGTGGTATTGCTCGCGGCCGTCTCACAGAGGAGCGAAAA
GCCTGGCGCAAGAATCATCCCCACGGTTTTGTGGCCAAGCCTGAAACCAA
AGATGGTTCCGTGAATTTGATGATCTGGGAGTGTATCATTCCTGGCAAGA
CTGGGACTGATTGGGAAGGTGGCTACTTTCCTCTTACTCTCTACTTCAGC
GAAGACTATCCCAGCAACCCCCAAAGTGTAAGTTTCCTCAAGGTTTCTTT
CATCCTAATGTCTACCCTTCTGGAACAGTATGCCTGTCAATCCTCAATGA
GGACAATGGGTGGAGACCTGCCATTACTGTGAAGCAAATTTTAGTGGGCA
TTCAGGATTTGCTGGGATCAACCCAATCCTGCTGATCCAGCACAAACTGA
TGGGATACCAGCTTTTCATCCAGGATCCTGCCGAGTACAAGAGAAGGGTG
CGACAGCAGGCCAAGCAATATCCCCCCTGTTATATAATGCA