CF417408

Overview
NameCF417408
Unique NameCF417408
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length701
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
CF417408_ssr186 microsatellite CF417408_ssr186:186..197. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig10901 contig Csv1_Contig10901:197..896. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF417408 vs. ExPASy Swiss-Prot
Match: G3P_PICCI (Glyceraldehyde-3-phosphate dehydrogenase OS=Pichia ciferrii GN=GPD PE=3 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 4.070e-33
Identity = 53/87 (60.92%), Postives = 63/87 (72.41%), Query Frame = 1 
Query:  439 RSIQVVSNRNPVNLPWGDLGIDLVIEGTGVFVDREGAGKHIQAGAKKVLITAPGKGDIPTYVVGVNADAYKPDEPIISNASCTTNCL 699
            + I+V   R+PVN+PWG  G+D VI+ TGVF + + A KHI AGAKKV+ITAP     P +VVGVN D Y PD  IISNASCTTNCL
Sbjct:   71 KKIRVYQERDPVNIPWGKDGVDYVIDSTGVFKELDSAQKHIDAGAKKVVITAP-SSTAPMFVVGVNEDKYTPDLNIISNASCTTNCL 156

HSP 2 Score: 54.299 bits (129), Expect = 4.070e-33
Identity = 34/72 (47.22%), Postives = 45/72 (62.50%), Query Frame = 3 
Query:  228 LKVAINGFGRIGRNFLRCWHGRKDSPLEVVAINDTG-GVKQASHLLKYDSTLGIFEADVKPVGTDGISVDGK 440
            + V INGFGRIGR  LR    RKD  +++VAIND     + AS++ KYDST G +  +V   G + I +DGK
Sbjct:    3 ITVGINGFGRIGRLVLRIALSRKD--IQIVAINDPFIAPEYASYMFKYDSTHGRYSGEVSHEG-ENIVIDGK 71
[Close]
BLAST of CF417408 vs. ExPASy Swiss-Prot
Match: G3P_CHLTR (Glyceraldehyde-3-phosphate dehydrogenase OS=Chlamydia trachomatis GN=gap PE=3 SV=3)

HSP 1 Score: 109.768 bits (273), Expect = 5.301e-33
Identity = 47/87 (54.02%), Postives = 66/87 (75.86%), Query Frame = 1 
Query:  439 RSIQVVSNRNPVNLPWGDLGIDLVIEGTGVFVDREGAGKHIQAGAKKVLITAPGKGDIPTYVVGVNADAYKPDEPIISNASCTTNCL 699
            R +  ++ ++   LPW DL +D+V+E TG+FV+R+   KH+ +GAK+VLITAP KGD+PT+V+GVN   + P + IISNASCTTNCL
Sbjct:   69 RKVHFLAEKDVQKLPWKDLDVDVVVESTGLFVNRDDVAKHLDSGAKRVLITAPAKGDVPTFVMGVNHQQFDPADVIISNASCTTNCL 155

HSP 2 Score: 51.9878 bits (123), Expect = 5.301e-33
Identity = 35/75 (46.67%), Postives = 46/75 (61.33%), Query Frame = 3 
Query:  228 LKVAINGFGRIGRNFLRCWHGRKDSPLEVVAINDTGGVKQASHLLKYDSTLGIFEADVKPVGTDGISVDG--KVH 446
            +++ INGFGRIGR  LR    +++SP+EVVAIND       ++L KYDST G F    +   +DG  V G  KVH
Sbjct:    1 MRIVINGFGRIGRLVLRQIL-KRNSPIEVVAINDLVAGDLLTYLFKYDSTHGSFAP--QATFSDGCLVMGERKVH 72
[Close]
BLAST of CF417408 vs. ExPASy Swiss-Prot
Match: G3P_PICPA (Glyceraldehyde-3-phosphate dehydrogenase OS=Pichia pastoris GN=GPD PE=3 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 6.904e-33
Identity = 55/87 (63.22%), Postives = 63/87 (72.41%), Query Frame = 1 
Query:  439 RSIQVVSNRNPVNLPWGDLGIDLVIEGTGVFVDREGAGKHIQAGAKKVLITAPGKGDIPTYVVGVNADAYKPDEPIISNASCTTNCL 699
            + I V   R+PVN+PWG  G+D VIE TGVF   EGA KHI AGAKKV+ITAP K D P +VVGVN + Y  D  I+SNASCTTNCL
Sbjct:   71 KEITVFQERDPVNIPWGKAGVDYVIESTGVFTTLEGAQKHIDAGAKKVVITAPSK-DAPMFVVGVNEEKYTSDLNIVSNASCTTNCL 156

HSP 2 Score: 49.2914 bits (116), Expect = 6.904e-33
Identity = 32/72 (44.44%), Postives = 45/72 (62.50%), Query Frame = 3 
Query:  228 LKVAINGFGRIGRNFLRCWHGRKDSPLEVVAINDTG-GVKQASHLLKYDSTLGIFEADVKPVGTDGISVDGK 440
            + V INGFGRIGR  LR    R D  ++VVAIND     + A+++ KYDST   ++ +V   G + I++DGK
Sbjct:    3 ITVGINGFGRIGRLVLRVALSRAD--IKVVAINDPFIAPEYAAYMFKYDSTHKAYKGEVSASG-NKINIDGK 71
[Close]
BLAST of CF417408 vs. ExPASy Swiss-Prot
Match: G3PC_TRYBB (Glyceraldehyde-3-phosphate dehydrogenase, cytosolic OS=Trypanosoma brucei brucei PE=1 SV=3)

HSP 1 Score: 106.301 bits (264), Expect = 2.587e-32
Identity = 50/93 (53.76%), Postives = 67/93 (72.04%), Query Frame = 1 
Query:  421 ASLLMARSIQVVSNRNPVNLPWGDLGIDLVIEGTGVFVDREGAGKHIQAGAKKVLITAPGKGDIPTYVVGVNADAYKPDEPIISNASCTTNCL 699
            A ++  + I+V S R+P NL W ++ +D+V+E TG+F+  + A KHIQAGAKKV+IT P K D P +V+GVN   YK  E I+SNASCTTNCL
Sbjct:   64 ALVVNGKKIRVTSERDPANLKWNEINVDVVVESTGLFLSDDTARKHIQAGAKKVVITGPSKDDTPMFVMGVNHTTYK-GEAIVSNASCTTNCL 155

HSP 2 Score: 53.1434 bits (126), Expect = 2.587e-32
Identity = 32/71 (45.07%), Postives = 42/71 (59.15%), Query Frame = 3 
Query:  228 LKVAINGFGRIGRNFLRCWHGRKDSPLEVVAINDTGGVKQASHLLKYDSTLGIFEADVKPVGTDGISVDGK 440
            ++V INGFGRIGR   R    R D  +E+V IND       +++LKYDST G FE  V+  G   + V+GK
Sbjct:    3 IRVGINGFGRIGRVVFRAAQRRND--IEIVGINDLLDADYMAYMLKYDSTHGRFEGAVEVQG-GALVVNGK 70
[Close]
BLAST of CF417408 vs. ExPASy Swiss-Prot
Match: G3P_CHLMU (Glyceraldehyde-3-phosphate dehydrogenase OS=Chlamydia muridarum GN=gap PE=3 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 7.440e-32
Identity = 48/88 (54.55%), Postives = 69/88 (78.41%), Query Frame = 1 
Query:  439 RSIQVVSNRNPVNLPWGDLGIDLVIEGTGVFVDREGAGKHIQAGAKKVLITAPGKGDIPTYVVGVNADAYKP-DEPIISNASCTTNCL 699
            R I+ ++ ++   LPW DL +D+V+E TG+FV+++ A KH+ +GAK+VLITAP KGD+PT+V+GVN   + P ++ IISNASCTTNCL
Sbjct:   69 RKIRFLAEKDVQKLPWKDLDVDVVVESTGLFVNKDDAAKHLDSGAKRVLITAPAKGDVPTFVMGVNHQKFDPANDVIISNASCTTNCL 156

HSP 2 Score: 50.447 bits (119), Expect = 7.440e-32
Identity = 31/71 (43.66%), Postives = 45/71 (63.38%), Query Frame = 3 
Query:  228 LKVAINGFGRIGRNFLRCWHGRKDSPLEVVAINDTGGVKQASHLLKYDSTLGIFEADVKPVGTDGISVDGK 440
            +++ INGFGRIGR  LR    ++++P+EVVAIND       ++L KYDST G F +  +   +DG  V G+
Sbjct:    1 MRIVINGFGRIGRLVLRQIL-KRNAPIEVVAINDLVSGDLLTYLFKYDSTHGSFAS--QATFSDGCLVVGE 68
[Close]
BLAST of CF417408 vs. ExPASy Swiss-Prot
Match: G3P_PICAN (Glyceraldehyde-3-phosphate dehydrogenase OS=Pichia angusta GN=GPD PE=3 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 2.140e-31
Identity = 53/91 (58.24%), Postives = 66/91 (72.53%), Query Frame = 1 
Query:  427 LLMARSIQVVSNRNPVNLPWGDLGIDLVIEGTGVFVDREGAGKHIQAGAKKVLITAPGKGDIPTYVVGVNADAYKPDEPIISNASCTTNCL 699
            ++  + I+V   R+P N+PWG  G+D V++ TGVF   EGA KHI AGAKKV+ITAP K D P +VVGVN + Y PD  I+SNASCTTNCL
Sbjct:   67 VINGKKIEVFQERDPANIPWGKEGVDYVLDSTGVFTTIEGAQKHIDAGAKKVIITAPSK-DAPMFVVGVNHEEYTPDIKILSNASCTTNCL 156

HSP 2 Score: 44.2838 bits (103), Expect = 2.140e-31
Identity = 29/61 (47.54%), Postives = 35/61 (57.38%), Query Frame = 3 
Query:  234 VAINGFGRIGRNFLRCWHGRKDSPLEVVAINDTG-GVKQASHLLKYDSTLGIFEADVKPVG 413
            V INGFGRIGR  LR    R D  + V+AIND       A+++ KYDST G F+  V   G
Sbjct:    5 VGINGFGRIGRLVLRIALSRDD--INVIAINDPFIAPDYAAYMFKYDSTHGKFKGTVTHEG 63
[Close]
BLAST of CF417408 vs. ExPASy Swiss-Prot
Match: G3P_DEBHA (Glyceraldehyde-3-phosphate dehydrogenase OS=Debaryomyces hansenii GN=GPD PE=3 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 2.786e-31
Identity = 52/87 (59.77%), Postives = 62/87 (71.26%), Query Frame = 1 
Query:  439 RSIQVVSNRNPVNLPWGDLGIDLVIEGTGVFVDREGAGKHIQAGAKKVLITAPGKGDIPTYVVGVNADAYKPDEPIISNASCTTNCL 699
            +SI+V   ++P ++PWG  G+D VIE TGVF   EGA KHI  GAKKV+ITAP   D P +VVGVN   Y PD  I+SNASCTTNCL
Sbjct:   71 QSIKVFGEKDPASIPWGKAGVDYVIESTGVFTTTEGAQKHIDGGAKKVIITAP-SSDAPMFVVGVNEQKYTPDLKIVSNASCTTNCL 156

HSP 2 Score: 46.595 bits (109), Expect = 2.786e-31
Identity = 29/72 (40.28%), Postives = 44/72 (61.11%), Query Frame = 3 
Query:  228 LKVAINGFGRIGRNFLRCWHGRKDSPLEVVAINDTG-GVKQASHLLKYDSTLGIFEADVKPVGTDGISVDGK 440
            + + INGFGRIGR  LR    R D  ++VVA+ND     + A+++ KYDST G ++ +V     D + +DG+
Sbjct:    3 ITIGINGFGRIGRLVLRIALQRSD--IKVVAVNDPFIAPEYAAYMFKYDSTHGRYKGEVTS-KDDSLVIDGQ 71
[Close]
BLAST of CF417408 vs. ExPASy Swiss-Prot
Match: G3P_BUCAI (Glyceraldehyde-3-phosphate dehydrogenase OS=Buchnera aphidicola subsp. Acyrthosiphon pisum GN=gapA PE=3 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 2.787e-31
Identity = 49/91 (53.85%), Postives = 65/91 (71.43%), Query Frame = 1 
Query:  427 LLMARSIQVVSNRNPVNLPWGDLGIDLVIEGTGVFVDREGAGKHIQAGAKKVLITAPGKGDIPTYVVGVNADAYKPDEPIISNASCTTNCL 699
            ++  ++I+V S ++P  L W DL ID+VIE TG+F+ RE A KHI AG+KKV++T P K DIP +V G N   Y+  E I+SNASCTTNCL
Sbjct:   66 IINGKNIRVTSIKDPEKLMWHDLSIDVVIESTGLFLKREQAYKHILAGSKKVVVTGPSKDDIPMFVRGANFHKYQ-GESIVSNASCTTNCL 155

HSP 2 Score: 56.9954 bits (136), Expect = 2.787e-31
Identity = 31/71 (43.66%), Postives = 45/71 (63.38%), Query Frame = 3 
Query:  228 LKVAINGFGRIGRNFLRCWHGRKDSPLEVVAINDTGGVKQASHLLKYDSTLGIFEADVKPVGTDGISVDGK 440
            +KV INGFGRIGR   R    +K S +E++AIND    +  +++LKYDST G+F+  +     D I ++GK
Sbjct:    3 IKVGINGFGRIGRVLFRL--AQKRSDIEIIAINDLLAPEYIAYMLKYDSTHGVFKKHI-TFDKDNIIINGK 70
[Close]
BLAST of CF417408 vs. ExPASy Swiss-Prot
Match: G3P_ZYMMO (Glyceraldehyde-3-phosphate dehydrogenase OS=Zymomonas mobilis GN=gap PE=3 SV=1)

HSP 1 Score: 95.9005 bits (237), Expect = 3.628e-31
Identity = 48/88 (54.55%), Postives = 61/88 (69.32%), Query Frame = 1 
Query:  439 RSIQVVSNRNPVNLPWGDLGIDLVIEGTGVFVDREGAGKHIQAGAKKVLITAPGKGDIP-TYVVGVNADAYKPDEPIISNASCTTNCL 699
            + I V + R+P NLP   LG+D+V+E TG+F + E A  H+ AGAKKVLI+AP KGD+  T V GVN      D+ I+SNASCTTNCL
Sbjct:   72 KKIVVTAERDPANLPHKKLGVDIVMECTGIFTNTEKASAHLTAGAKKVLISAPAKGDVDRTVVYGVNHKDLTADDKIVSNASCTTNCL 159

HSP 2 Score: 59.6918 bits (143), Expect = 3.628e-31
Identity = 35/71 (49.30%), Postives = 42/71 (59.15%), Query Frame = 3 
Query:  228 LKVAINGFGRIGRNFLRCWHGRKDSPLEVVAINDTGGVKQASHLLKYDSTLGIFEADVKPVGTDGISVDGK 440
            +KVAINGFGRIGR   R    R DS LE+V IND G V+  + L K DS  G +   V   G D + +DGK
Sbjct:    3 VKVAINGFGRIGRLAARAILSRPDSGLELVTINDLGSVEGNAFLFKRDSAHGTYPGTVTTEGNDMV-IDGK 72
[Close]
BLAST of CF417408 vs. ExPASy Swiss-Prot
Match: G3P_PSEAE (Glyceraldehyde-3-phosphate dehydrogenase OS=Pseudomonas aeruginosa GN=gap PE=3 SV=3)

HSP 1 Score: 97.8265 bits (242), Expect = 6.152e-31
Identity = 48/90 (53.33%), Postives = 60/90 (66.67%), Query Frame = 1 
Query:  430 LMARSIQVVSNRNPVNLPWGDLGIDLVIEGTGVFVDREGAGKHIQAGAKKVLITAPGKGDIPTYVVGVNADAYKPDEPIISNASCTTNCL 699
            +M   I V + RNP  LPW  LG+D+V+E TG+F  R+ A  H+QAGA KVLI+APGK    T V GVN +  +    I+SNASCTTNCL
Sbjct:   70 VMGDRIAVSAIRNPAELPWKSLGVDIVLECTGLFTSRDKAAAHLQAGAGKVLISAPGKDVEATVVYGVNHEVLRASHRIVSNASCTTNCL 159

HSP 2 Score: 56.9954 bits (136), Expect = 6.152e-31
Identity = 30/60 (50.00%), Postives = 39/60 (65.00%), Query Frame = 3 
Query:  228 LKVAINGFGRIGRNFLRCWH-GRKDSPLEVVAINDTGGVKQASHLLKYDSTLGIFEADVK 404
            +++AINGFGRIGRN LR  + G     L+VVAIND G     +HL +YDS  G F  +V+
Sbjct:    3 IRLAINGFGRIGRNVLRALYTGHYREQLQVVAINDLGDAAVNAHLFQYDSVHGHFPGEVE 62
The following BLAST results are available for this feature:
BLAST of CF417408 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 348
Match NameE-valueIdentityDescription
G3P_PICCI4.070e-3360.92Glyceraldehyde-3-phosphate dehydrogenase OS=Pichia... [more]
G3P_CHLTR5.301e-3354.02Glyceraldehyde-3-phosphate dehydrogenase OS=Chlamy... [more]
G3P_PICPA6.904e-3363.22Glyceraldehyde-3-phosphate dehydrogenase OS=Pichia... [more]
G3PC_TRYBB2.587e-3253.76Glyceraldehyde-3-phosphate dehydrogenase, cytosoli... [more]
G3P_CHLMU7.440e-3254.55Glyceraldehyde-3-phosphate dehydrogenase OS=Chlamy... [more]
G3P_PICAN2.140e-3158.24Glyceraldehyde-3-phosphate dehydrogenase OS=Pichia... [more]
G3P_DEBHA2.786e-3159.77Glyceraldehyde-3-phosphate dehydrogenase OS=Debary... [more]
G3P_BUCAI2.787e-3153.85Glyceraldehyde-3-phosphate dehydrogenase OS=Buchne... [more]
G3P_ZYMMO3.628e-3154.55Glyceraldehyde-3-phosphate dehydrogenase OS=Zymomo... [more]
G3P_PSEAE6.152e-3153.33Glyceraldehyde-3-phosphate dehydrogenase OS=Pseudo... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_115000-338 Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange Citrus sinensis cDNA clone IDFL-004_H05 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF417408 ID=CF417408; Name=CF417408; organism=Citrus sinensis; type=EST; length=701bp
GCATCATCCAGCAAATACCTATCATGGCTTCGGCCACTCTCTCTGTAGCC
AAATCAGCTCTTCAGGGAAATGGTAAGGGATTCTCAGAATTCTCAGGTCT
ACGCAACTCTGCAAGCCTTCCTTTTGGCAGGAAATCTTCTGATGACTTCC
ATTCAGTCATTGCTCTCCAGACCTCTGCTTTAGGAAGCAGCAGCAGCGGG
TACAGGAAGGTTGCTGCTCAGGCAAAGCTGAAGGTTGCAATCAATGGGTT
TGGTAGAATTGGTAGGAACTTCTTGAGGTGCTGGCACGGCCGCAAGGACT
CTCCACTTGAAGTCGTTGCCATCAATGACACCGGAGGTGTCAAGCAGGCC
TCTCACCTCCTCAAGTACGACTCCACCCTTGGCATCTTTGAAGCCGATGT
TAAACCAGTTGGTACTGATGGCATCTCTGTTGATGGCAAGGTCCATCCAG
GTTGTTTCTAACCGCAACCCCGTCAATCTCCCCTGGGGGGACTTGGGCAT
CGACCTTGTGATTGAAGGGACAGGAGTGTTTGTAGACAGAGAGGGTGCGG
GCAAGCACATTCAGGCTGGTGCCAAGAAAGTACTTATTACAGCCCCCGGC
AAGGGTGACATCCCAACCTACGTTGTTGGTGTCAATGCGGATGCTTACAA
GCCAGATGAGCCCATCATCAGCAATGCTTCTTGCACAACCAACTGCCTTG
C
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