CF417557 | Citrus Genome Database

Overview


Name	CF417557
Unique Name	CF417557
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	712

Libraries


Library Name	Type
Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

BLAST of CF417557 vs. ExPASy Swiss-Prot
Match: PTH_SULIN (Peptidyl-tRNA hydrolase OS=Sulfolobus islandicus (strain Y.N.15.51 / Yellowstone #2) GN=pth PE=3 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 2.522e-16
Identity = 28/66 (42.42%), Postives = 42/66 (63.64%), Query Frame = 3

Query:  297 LKLALVVRQDLKMGSGKIASQCAHAATGMYAELMQSD----RHLLRKWEQCGQPKIVVTCKNQQEM 482
            +K+ +VVR D+KMG GK+A+Q AHAA  +   ++ S+    +  L +W Q GQPKIVV   +  E+
Sbjct:    2 VKMVIVVRSDIKMGKGKMAAQVAHAAVTLVISIINSNNSRWKEWLNEWLQQGQPKIVVKANSLDEI 67

HSP 2 Score: 50.447 bits (119), Expect = 2.522e-16
Identity = 22/47 (46.81%), Postives = 32/47 (68.09%), Query Frame = 2

Query:  509 AENTGLPTFVVADAGRTQVSAGSKTVLAIGPGPKSLVDSVTGKQRLL 649
            AE   LP  ++ DAG+TQ+  G+ T L IGP P++L+D +TG  +LL
Sbjct:   74 AETMNLPFSIIEDAGKTQLEPGTITCLGIGPAPENLIDPITGDLKLL 120

BLAST of CF417557 vs. ExPASy Swiss-Prot
Match: PTH_SULIM (Peptidyl-tRNA hydrolase OS=Sulfolobus islandicus (strain M.14.25 / Kamchatka #1) GN=pth PE=3 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 2.522e-16
Identity = 28/66 (42.42%), Postives = 42/66 (63.64%), Query Frame = 3

Query:  297 LKLALVVRQDLKMGSGKIASQCAHAATGMYAELMQSD----RHLLRKWEQCGQPKIVVTCKNQQEM 482
            +K+ +VVR D+KMG GK+A+Q AHAA  +   ++ S+    +  L +W Q GQPKIVV   +  E+
Sbjct:    2 VKMVIVVRSDIKMGKGKMAAQVAHAAVTLVISIINSNNSRWKEWLNEWLQQGQPKIVVKANSLDEI 67

HSP 2 Score: 50.447 bits (119), Expect = 2.522e-16
Identity = 22/47 (46.81%), Postives = 32/47 (68.09%), Query Frame = 2

Query:  509 AENTGLPTFVVADAGRTQVSAGSKTVLAIGPGPKSLVDSVTGKQRLL 649
            AE   LP  ++ DAG+TQ+  G+ T L IGP P++L+D +TG  +LL
Sbjct:   74 AETMNLPFSIIEDAGKTQLEPGTITCLGIGPAPENLIDPITGDLKLL 120

BLAST of CF417557 vs. ExPASy Swiss-Prot
Match: PTH_SULIL (Peptidyl-tRNA hydrolase OS=Sulfolobus islandicus (strain L.S.2.15 / Lassen #1) GN=pth PE=3 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 2.522e-16
Identity = 28/66 (42.42%), Postives = 42/66 (63.64%), Query Frame = 3

Query:  297 LKLALVVRQDLKMGSGKIASQCAHAATGMYAELMQSD----RHLLRKWEQCGQPKIVVTCKNQQEM 482
            +K+ +VVR D+KMG GK+A+Q AHAA  +   ++ S+    +  L +W Q GQPKIVV   +  E+
Sbjct:    2 VKMVIVVRSDIKMGKGKMAAQVAHAAVTLVISIINSNNSRWKEWLNEWLQQGQPKIVVKANSLDEI 67

HSP 2 Score: 50.447 bits (119), Expect = 2.522e-16
Identity = 22/47 (46.81%), Postives = 32/47 (68.09%), Query Frame = 2

Query:  509 AENTGLPTFVVADAGRTQVSAGSKTVLAIGPGPKSLVDSVTGKQRLL 649
            AE   LP  ++ DAG+TQ+  G+ T L IGP P++L+D +TG  +LL
Sbjct:   74 AETMNLPFSIIEDAGKTQLEPGTITCLGIGPAPENLIDPITGDLKLL 120

BLAST of CF417557 vs. ExPASy Swiss-Prot
Match: PTH_SULIK (Peptidyl-tRNA hydrolase OS=Sulfolobus islandicus (strain M.16.4 / Kamchatka #3) GN=pth PE=3 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 2.522e-16
Identity = 28/66 (42.42%), Postives = 42/66 (63.64%), Query Frame = 3

Query:  297 LKLALVVRQDLKMGSGKIASQCAHAATGMYAELMQSD----RHLLRKWEQCGQPKIVVTCKNQQEM 482
            +K+ +VVR D+KMG GK+A+Q AHAA  +   ++ S+    +  L +W Q GQPKIVV   +  E+
Sbjct:    2 VKMVIVVRSDIKMGKGKMAAQVAHAAVTLVISIINSNNSRWKEWLNEWLQQGQPKIVVKANSLDEI 67

HSP 2 Score: 50.447 bits (119), Expect = 2.522e-16
Identity = 22/47 (46.81%), Postives = 32/47 (68.09%), Query Frame = 2

Query:  509 AENTGLPTFVVADAGRTQVSAGSKTVLAIGPGPKSLVDSVTGKQRLL 649
            AE   LP  ++ DAG+TQ+  G+ T L IGP P++L+D +TG  +LL
Sbjct:   74 AETMNLPFSIIEDAGKTQLEPGTITCLGIGPAPENLIDPITGDLKLL 120

BLAST of CF417557 vs. ExPASy Swiss-Prot
Match: PTH_SULIA (Peptidyl-tRNA hydrolase OS=Sulfolobus islandicus (strain M.16.27) GN=pth PE=3 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 2.522e-16
Identity = 28/66 (42.42%), Postives = 42/66 (63.64%), Query Frame = 3

Query:  297 LKLALVVRQDLKMGSGKIASQCAHAATGMYAELMQSD----RHLLRKWEQCGQPKIVVTCKNQQEM 482
            +K+ +VVR D+KMG GK+A+Q AHAA  +   ++ S+    +  L +W Q GQPKIVV   +  E+
Sbjct:    2 VKMVIVVRSDIKMGKGKMAAQVAHAAVTLVISIINSNNSRWKEWLNEWLQQGQPKIVVKANSLDEI 67

HSP 2 Score: 50.447 bits (119), Expect = 2.522e-16
Identity = 22/47 (46.81%), Postives = 32/47 (68.09%), Query Frame = 2

Query:  509 AENTGLPTFVVADAGRTQVSAGSKTVLAIGPGPKSLVDSVTGKQRLL 649
            AE   LP  ++ DAG+TQ+  G+ T L IGP P++L+D +TG  +LL
Sbjct:   74 AETMNLPFSIIEDAGKTQLEPGTITCLGIGPAPENLIDPITGDLKLL 120

BLAST of CF417557 vs. ExPASy Swiss-Prot
Match: PTH2_DICDI (Probable peptidyl-tRNA hydrolase 2 OS=Dictyostelium discoideum GN=pth2 PE=3 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 1.160e-15
Identity = 24/63 (38.10%), Postives = 38/63 (60.32%), Query Frame = 3

Query:  294 DLKLALVVRQDLKMGSGKIASQCAHAATGMYAELMQSDRHLLRKWEQCGQPKIVVTCKNQQEM 482
            D K  LV+R DL M  GKI +QC HA+ G   + ++  + +LR+WE  G  KI +   +++E+
Sbjct:   95 DCKQVLVIRTDLGMTKGKIIAQCCHASLGARQKALKDSKKVLRQWENFGCAKITLKANSKEEL 157

HSP 2 Score: 50.0618 bits (118), Expect = 1.160e-15
Identity = 25/53 (47.17%), Postives = 36/53 (67.92%), Query Frame = 2

Query:  488 RNKLWEVAENTGLPTFVVADAGRTQVSAGSKTVLAIGPGPKSLVDSVTGKQRL 646
            RNK    A+  G+ +++V D+G TQ+ +GS TVLA+GP PK  VD+VT   +L
Sbjct:  161 RNK----AKELGVVSYLVLDSGHTQIPSGSATVLAVGPAPKEAVDAVTKHLKL 209

BLAST of CF417557 vs. ExPASy Swiss-Prot
Match: PTH2_YEAST (Peptidyl-tRNA hydrolase 2 OS=Saccharomyces cerevisiae GN=PTH2 PE=1 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 1.507e-15
Identity = 24/52 (46.15%), Postives = 38/52 (73.08%), Query Frame = 2

Query:  491 NKLWEVAENTGLPTFVVADAGRTQVSAGSKTVLAIGPGPKSLVDSVTGKQRL 646
            ++L+  A + G+   V+ DAGRTQ++AGS TVL +GP PK+++D +TG  +L
Sbjct:  162 DELYAKAISLGVNAAVIHDAGRTQIAAGSATVLGLGPAPKAVLDQITGDLKL 213

HSP 2 Score: 49.6766 bits (117), Expect = 1.507e-15
Identity = 24/69 (34.78%), Postives = 39/69 (56.52%), Query Frame = 3

Query:  294 DLKLALVVRQDLKMGSGKIASQCAHAATGMYAELMQSDRH------LLRKWEQCGQPKIVVTCKNQQEM 482
            ++++ALV+RQDL M  GKIA+QC HAA   +  +  +         + ++W   GQ KI + C ++  M
Sbjct:   93 EVRMALVIRQDLGMTKGKIAAQCCHAALSCFRHIATNPARASYNPIMTQRWLNAGQAKITLKCPDKFTM 161

BLAST of CF417557 vs. ExPASy Swiss-Prot
Match: PTH_PYRKO (Peptidyl-tRNA hydrolase OS=Pyrococcus kodakaraensis GN=pth PE=3 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 4.578e-15
Identity = 29/52 (55.77%), Postives = 37/52 (71.15%), Query Frame = 2

Query:  494 KLWEVAENTGLPTFVVADAGRTQVSAGSKTVLAIGPGPKSLVDSVTGKQRLL 649
            KL   AE  GLPT ++ DAG T++  G+ TVLAIGPGP+ +VD VTG  +LL
Sbjct:   67 KLKAEAEKLGLPTALIKDAGLTEIPPGTVTVLAIGPGPEEVVDKVTGHLKLL 118

HSP 2 Score: 42.743 bits (99), Expect = 4.578e-15
Identity = 21/61 (34.43%), Postives = 31/61 (50.82%), Query Frame = 3

Query:  300 KLALVVRQDLKMGSGKIASQCAHAATGMYAELMQSDRHLLRKWEQCGQPKIVVTCKNQQEM 482
            K  +V R+DLK+  GK A Q AH A     +  +      + W   GQ K+VV  +N +E+
Sbjct:    5 KQVIVARKDLKLSKGKFAVQVAHGAVTAAIKAQKEKPEWFKAWFHEGQKKVVVKAENLEEL 65

BLAST of CF417557 vs. ExPASy Swiss-Prot
Match: PTH_PYRAB (Peptidyl-tRNA hydrolase OS=Pyrococcus abyssi GN=pth PE=3 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 5.879e-15
Identity = 27/52 (51.92%), Postives = 35/52 (67.31%), Query Frame = 2

Query:  494 KLWEVAENTGLPTFVVADAGRTQVSAGSKTVLAIGPGPKSLVDSVTGKQRLL 649
            KL   AE   LPT ++ DAG T++  G+ TVLAIGPGP  ++D VTG  +LL
Sbjct:   70 KLKAEAEKLNLPTALIQDAGLTEIPPGTITVLAIGPGPSEIIDKVTGHLKLL 121

HSP 2 Score: 45.4394 bits (106), Expect = 5.879e-15
Identity = 23/61 (37.70%), Postives = 33/61 (54.10%), Query Frame = 3

Query:  300 KLALVVRQDLKMGSGKIASQCAHAATGMYAELMQSDRHLLRKWEQCGQPKIVVTCKNQQEM 482
            K  +VVR+DLK+  GK+A Q AH A     +  +  R    KW   GQ K+VV  ++  E+
Sbjct:    8 KQVIVVRKDLKLSKGKLAVQVAHGAVTAAFKAYKEKREWFEKWFHEGQKKVVVKVESLDEL 68

BLAST of CF417557 vs. ExPASy Swiss-Prot
Match: PTH_PYRHO (Peptidyl-tRNA hydrolase OS=Pyrococcus horikoshii GN=pth PE=1 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 5.879e-15
Identity = 26/52 (50.00%), Postives = 35/52 (67.31%), Query Frame = 2

Query:  494 KLWEVAENTGLPTFVVADAGRTQVSAGSKTVLAIGPGPKSLVDSVTGKQRLL 649
            KL   AE  GLP  ++ DAG T++  G+ TVLA+GP P+ +VD VTG  +LL
Sbjct:   70 KLKAEAEKLGLPNALIRDAGLTEIPPGTVTVLAVGPAPEEIVDKVTGNLKLL 121

HSP 2 Score: 46.2098 bits (108), Expect = 5.879e-15
Identity = 22/61 (36.07%), Postives = 34/61 (55.74%), Query Frame = 3

Query:  300 KLALVVRQDLKMGSGKIASQCAHAATGMYAELMQSDRHLLRKWEQCGQPKIVVTCKNQQEM 482
            K  +V R DLK+  GK+A+Q AH A     E  +  R     W + GQ K+VV  ++++E+
Sbjct:    8 KQVIVARADLKLSKGKLAAQVAHGAVTAAFEAYKKKREWFEAWFREGQKKVVVKVESEEEL 68

The following BLAST results are available for this feature:

BLAST of CF417557 vs. ExPASy Swiss-Prot

BLAST of CF417557 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 42


Match Name	E-value	Identity	Description
PTH_SULIN	2.522e-16	42.42	Peptidyl-tRNA hydrolase OS=Sulfolobus islandicus (...	[more]
PTH_SULIM	2.522e-16	42.42	Peptidyl-tRNA hydrolase OS=Sulfolobus islandicus (...	[more]
PTH_SULIL	2.522e-16	42.42	Peptidyl-tRNA hydrolase OS=Sulfolobus islandicus (...	[more]
PTH_SULIK	2.522e-16	42.42	Peptidyl-tRNA hydrolase OS=Sulfolobus islandicus (...	[more]
PTH_SULIA	2.522e-16	42.42	Peptidyl-tRNA hydrolase OS=Sulfolobus islandicus (...	[more]
PTH2_DICDI	1.160e-15	38.10	Probable peptidyl-tRNA hydrolase 2 OS=Dictyosteliu...	[more]
PTH2_YEAST	1.507e-15	46.15	Peptidyl-tRNA hydrolase 2 OS=Saccharomyces cerevis...	[more]
PTH_PYRKO	4.578e-15	55.77	Peptidyl-tRNA hydrolase OS=Pyrococcus kodakaraensi...	[more]
PTH_PYRAB	5.879e-15	51.92	Peptidyl-tRNA hydrolase OS=Pyrococcus abyssi GN=pt...	[more]
PTH_PYRHO	5.879e-15	50.00	Peptidyl-tRNA hydrolase OS=Pyrococcus horikoshii G...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_115000-487 Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange Citrus sinensis cDNA clone IDFL-006_F01 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF417557 ID=CF417557; Name=CF417557; organism=Citrus sinensis; type=EST; length=712bp
GTCCGTGCAACTGTTAACCACGCAATCAGTAATCTGTTCATAATCTGTTA
ACGACGATGTGGGCTTCCCAGCAAAACTCTTCTCAGCTTTCTAACAAGAA
GCAGCAGAAGCAGGAAAAAGTATCGTTAGCAGTGAGTTTTAGGCCAGAGA
ATTTCATCCCAGGCCTTGTAATTGGTTTCATTTTTGGGATGTTATTAGAT
TTATCAAAGCCAGGTAAGAATTATGTGAAAAAGAAAAATTTCTTACCGAG
GAAACCACAAGAACAAGTTTCTGTCTCAAGTGACGGTGACCAAGACCTCA
AATTGGCTTTGGTGGTTAGGCAAGACCTGAAGATGGGGAGTGGAAAGATT
GCATCACAATGTGCCCATGCAGCCACTGGGATGTATGCAGAACTAATGCA
AAGTGACCGGCACCTTCTGAGAAAATGGGAGCAATGTGGGCAGCCCAAGA
TAGTTGTTACGTGCAAGAATCAACAAGAAATGTAAGCAGGAATAAGCTGT
GGGAAGTAGCTGAAAACACGGGCCTTCCAACTTTTGTTGTTGCGGATGCT
GGACGGACACAGGTTTCAGCTGGGTCGAAGACGGTTCTTGCCATCGGACC
TGGACCCAAATCTTTGGTGGATTCTGTGACAGGGAAACAGCGCCTGCTCT
AGTTATGCTTAAGAATGGAGGACTTTTTGCTTAAATTTCCCCGGATGACA
ATGTTAAAAGAT