CF417557

Overview
NameCF417557
Unique NameCF417557
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length712
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CF417557 vs. ExPASy Swiss-Prot
Match: PTH_CALMQ (Peptidyl-tRNA hydrolase OS=Caldivirga maquilingensis (strain DSMZ 13496 / IC-167) GN=pth PE=3 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 9.932e-15
Identity = 26/47 (55.32%), Postives = 35/47 (74.47%), Query Frame = 2 
Query:  509 AENTGLPTFVVADAGRTQVSAGSKTVLAIGPGPKSLVDSVTGKQRLL 649
            AE+ GLPT ++ DAG T++  G+ T LAIGPGP+ LV+ VTG  +LL
Sbjct:   72 AESLGLPTALIEDAGLTELPPGTVTALAIGPGPEELVNKVTGSLKLL 118

HSP 2 Score: 45.0542 bits (105), Expect = 9.932e-15
Identity = 22/63 (34.92%), Postives = 36/63 (57.14%), Query Frame = 3 
Query:  294 DLKLALVVRQDLKMGSGKIASQCAHAATGMYAELMQSDRHLLRKWEQCGQPKIVVTCKNQQEM 482
            + K ++VVR DL+M  GK+A Q AHAA     E +++    +  W   GQ KIV+   + +++
Sbjct:    3 EFKQSIVVRGDLRMSRGKLAVQVAHAAVSAVLEALRNRGDWVNAWVSGGQKKIVLVVDSVEDL 65
[Close]
BLAST of CF417557 vs. ExPASy Swiss-Prot
Match: PTH_HALSA (Peptidyl-tRNA hydrolase OS=Halobacterium salinarium GN=pth PE=3 SV=1)

HSP 1 Score: 53.1434 bits (126), Expect = 2.208e-14
Identity = 24/44 (54.55%), Postives = 32/44 (72.73%), Query Frame = 2 
Query:  503 EVAENTGLPTFVVADAGRTQVSAGSKTVLAIGPGPKSLVDSVTG 634
            E A+  GLPT +++DAGRTQ+  G+ T LAIGP P + VD +TG
Sbjct:   64 EEAKRAGLPTGLISDAGRTQLEPGTPTALAIGPAPDADVDQITG 107

HSP 2 Score: 46.595 bits (109), Expect = 2.208e-14
Identity = 22/62 (35.48%), Postives = 37/62 (59.68%), Query Frame = 3 
Query:  297 LKLALVVRQDLKMGSGKIASQCAHAATGMYAELMQSDRHLLRKWEQCGQPKIVVTCKNQQEM 482
            +K  +  R D+ MG GK+A+Q AHA+   Y     +D   +R+W+  GQ K+VV   +++E+
Sbjct:    1 MKQVIAARTDIGMGQGKLAAQVAHASLNAYE---YTDDRAVRRWKDEGQTKVVVKVGSEREL 59
[Close]
BLAST of CF417557 vs. ExPASy Swiss-Prot
Match: PTH_HALS3 (Peptidyl-tRNA hydrolase OS=Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1) GN=pth PE=3 SV=1)

HSP 1 Score: 53.1434 bits (126), Expect = 2.208e-14
Identity = 24/44 (54.55%), Postives = 32/44 (72.73%), Query Frame = 2 
Query:  503 EVAENTGLPTFVVADAGRTQVSAGSKTVLAIGPGPKSLVDSVTG 634
            E A+  GLPT +++DAGRTQ+  G+ T LAIGP P + VD +TG
Sbjct:   64 EEAKRAGLPTGLISDAGRTQLEPGTPTALAIGPAPDADVDQITG 107

HSP 2 Score: 46.595 bits (109), Expect = 2.208e-14
Identity = 22/62 (35.48%), Postives = 37/62 (59.68%), Query Frame = 3 
Query:  297 LKLALVVRQDLKMGSGKIASQCAHAATGMYAELMQSDRHLLRKWEQCGQPKIVVTCKNQQEM 482
            +K  +  R D+ MG GK+A+Q AHA+   Y     +D   +R+W+  GQ K+VV   +++E+
Sbjct:    1 MKQVIAARTDIGMGQGKLAAQVAHASLNAYE---YTDDRAVRRWKDEGQTKVVVKVGSEREL 59
[Close]
BLAST of CF417557 vs. ExPASy Swiss-Prot
Match: PTH_THEON (Peptidyl-tRNA hydrolase OS=Thermococcus onnurineus (strain NA1) GN=pth PE=3 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 4.849e-14
Identity = 23/47 (48.94%), Postives = 33/47 (70.21%), Query Frame = 2 
Query:  509 AENTGLPTFVVADAGRTQVSAGSKTVLAIGPGPKSLVDSVTGKQRLL 649
            AE  G+PT ++ DAG T++  G+ T LA+GP P+ LVD VTG  +L+
Sbjct:   72 AEKLGIPTALIRDAGLTEIPPGTITCLAVGPAPEELVDKVTGHLKLV 118

HSP 2 Score: 46.2098 bits (108), Expect = 4.849e-14
Identity = 21/61 (34.43%), Postives = 35/61 (57.38%), Query Frame = 3 
Query:  300 KLALVVRQDLKMGSGKIASQCAHAATGMYAELMQSDRHLLRKWEQCGQPKIVVTCKNQQEM 482
            K  +V+R+DLK+  GK+A Q AH A     ++ +      + W   GQ K+VV  +N++E+
Sbjct:    5 KQVMVIRKDLKLSKGKMAVQVAHGAVTAALKVQKEKPEWFKAWFHEGQKKVVVKAENEREL 65
[Close]
BLAST of CF417557 vs. ExPASy Swiss-Prot
Match: PTH_METKA (Peptidyl-tRNA hydrolase OS=Methanopyrus kandleri GN=pth PE=3 SV=1)

HSP 1 Score: 50.447 bits (119), Expect = 6.303e-14
Identity = 24/61 (39.34%), Postives = 40/61 (65.57%), Query Frame = 3 
Query:  300 KLALVVRQDLKMGSGKIASQCAHAATGMYAELMQSDRHLLRKWEQCGQPKIVVTCKNQQEM 482
            K  +VVR DLK+  GK+A+Q AHA+ G +    +S    + +W + GQ K+V+ CK+++E+
Sbjct:    3 KQVIVVRGDLKLSRGKLAAQVAHASLGAFLRAKESGAP-VEEWLREGQKKVVLKCKDKEEL 62

HSP 2 Score: 47.7506 bits (112), Expect = 6.303e-14
Identity = 24/52 (46.15%), Postives = 34/52 (65.38%), Query Frame = 2 
Query:  494 KLWEVAENTGLPTFVVADAGRTQVSAGSKTVLAIGPGPKSLVDSVTGKQRLL 649
            +L E+A+  GLP+F+V DAG T++  G+ T L IGP  +  +D VTG   LL
Sbjct:   64 ELHELAKRRGLPSFLVRDAGLTELEPGTVTCLGIGPEREEEIDRVTGDLPLL 115
[Close]
BLAST of CF417557 vs. ExPASy Swiss-Prot
Match: PTH_METTH (Peptidyl-tRNA hydrolase OS=Methanobacterium thermoautotrophicum GN=pth PE=3 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 8.191e-14
Identity = 24/62 (38.71%), Postives = 39/62 (62.90%), Query Frame = 3 
Query:  297 LKLALVVRQDLKMGSGKIASQCAHAATGMYAELMQSDRHLLRKWEQCGQPKIVVTCKNQQEM 482
            +K  ++VR DLKMG GKIA+Q  HA+ G +    +++   +RKWE  G  K++V+  +  E+
Sbjct:    1 MKQVIIVRSDLKMGKGKIAAQACHASIGSF---KRTEEDKIRKWELEGSKKVIVSVNSLDEL 59

HSP 2 Score: 45.4394 bits (106), Expect = 8.191e-14
Identity = 19/52 (36.54%), Postives = 31/52 (59.62%), Query Frame = 2 
Query:  494 KLWEVAENTGLPTFVVADAGRTQVSAGSKTVLAIGPGPKSLVDSVTGKQRLL 649
            +++   +  G+  ++V DAG TQ+ AG+ T L IGP     +D +TG  +LL
Sbjct:   61 EIYRAVKEAGISNYLVRDAGHTQIPAGTITCLGIGPDDDEKIDKITGDLKLL 112
[Close]
BLAST of CF417557 vs. ExPASy Swiss-Prot
Match: PTH2_CAEEL (Probable peptidyl-tRNA hydrolase 2 OS=Caenorhabditis elegans GN=C24G6.8 PE=2 SV=1)

HSP 1 Score: 53.5286 bits (127), Expect = 1.657e-13
Identity = 28/77 (36.36%), Postives = 46/77 (59.74%), Query Frame = 3 
Query:  258 QEQVSVSSDGDQDLKLALVVRQDLKMGSGKIASQCAHAATGMYAELMQSD--RHLLRKWEQCGQPKIVVTCKNQQEM 482
            +E  +V S   +  K+ LV    LKMG+GKIA+Q  HA  G+Y + M S+  ++ +  W + GQ KIVV  ++ +++
Sbjct:  188 EEMGAVQSVAGRTHKMVLVANMSLKMGTGKIAAQVGHATLGVYRQAMNSENGQNAIAAWTRHGQVKIVVKGQSTEQL 264

HSP 2 Score: 43.1282 bits (100), Expect = 1.657e-13
Identity = 26/51 (50.98%), Postives = 34/51 (66.67%), Query Frame = 2 
Query:  497 LWEVAENTGLPTFVVADAGRTQVSAGSKTVLAIGPGPKSLVDSVTGKQRLL 649
            L +VA++ G   ++V DAG TQ+ AGS+TVL I  G    VDSVTG  +LL
Sbjct:  267 LCKVAKDAGCYYYLVQDAGYTQIPAGSRTVLGIF-GTVEQVDSVTGGLKLL 316
[Close]
BLAST of CF417557 vs. ExPASy Swiss-Prot
Match: PTH_ARCFU (Peptidyl-tRNA hydrolase OS=Archaeoglobus fulgidus GN=pth PE=1 SV=1)

HSP 1 Score: 51.9878 bits (123), Expect = 1.125e-12
Identity = 29/62 (46.77%), Postives = 39/62 (62.90%), Query Frame = 3 
Query:  297 LKLALVVRQDLKMGSGKIASQCAHAATGMYAELMQSDRHLLRKWEQCGQPKIVVTCKNQQEM 482
            LK  +VVR DLK+  GK+A Q AHAA   Y   ++SD  L RKW   GQ K+V+  K+ +E+
Sbjct:    3 LKQVIVVRDDLKLSRGKLAVQVAHAAIIGY---LKSDSSLRRKWLDEGQKKVVLKVKSLEEL 61

HSP 2 Score: 41.9726 bits (97), Expect = 1.125e-12
Identity = 22/47 (46.81%), Postives = 29/47 (61.70%), Query Frame = 2 
Query:  509 AENTGLPTFVVADAGRTQVSAGSKTVLAIGPGPKSLVDSVTGKQRLL 649
            AE+ GL T +V DAG T+V  G+ T + IGP  +  +D VTG   LL
Sbjct:   68 AESLGLVTGLVQDAGLTEVPPGTITAVVIGPDEERKIDKVTGNLPLL 114
[Close]
BLAST of CF417557 vs. ExPASy Swiss-Prot
Match: PTH_PYRAE (Peptidyl-tRNA hydrolase OS=Pyrobaculum aerophilum GN=pth PE=3 SV=1)

HSP 1 Score: 52.7582 bits (125), Expect = 1.442e-12
Identity = 25/51 (49.02%), Postives = 34/51 (66.67%), Query Frame = 2 
Query:  494 KLWEVAENTGLPTFVVADAGRTQVSAGSKTVLAIGPGPKSLVDSVTGKQRL 646
            +L E A++ GLPT VV DAG T++  G+ T + +GP P  LVD VTG  +L
Sbjct:   68 QLHERAKSLGLPTAVVIDAGLTELPPGTPTAICVGPAPDELVDKVTGSLKL 118

HSP 2 Score: 40.817 bits (94), Expect = 1.442e-12
Identity = 23/58 (39.66%), Postives = 32/58 (55.17%), Query Frame = 3 
Query:  294 DLKLALVVRQDLKMGSGKIASQCAHAATGMYAELMQSD--RHLLRKWEQCGQPKIVVT 461
            D+K+ + +R DLK+  GK A+Q  HAA       M     R  L +W + GQ KIV+T
Sbjct:    2 DVKMTIAIRGDLKISCGKAAAQAGHAAVECVLAAMGDAKWRKWLDQWLEEGQKKIVLT 59
[Close]
BLAST of CF417557 vs. ExPASy Swiss-Prot
Match: PTH_THESM (Peptidyl-tRNA hydrolase OS=Thermococcus sibiricus (strain MM 739 / DSM 12597) GN=pth PE=3 SV=1)

HSP 1 Score: 51.2174 bits (121), Expect = 1.462e-12
Identity = 24/58 (41.38%), Postives = 39/58 (67.24%), Query Frame = 2 
Query:  485 SRNKLWEV---AENTGLPTFVVADAGRTQVSAGSKTVLAIGPGPKSLVDSVTGKQRLL 649
            S  +L+E+   AE  G+P  ++ DAG T++  G+ T LAIGPGP+ +V+ +TG  +L+
Sbjct:   61 SERELFELKAEAEKLGIPNSLIRDAGLTEIPPGTITCLAIGPGPEEIVNKITGNLKLV 118

HSP 2 Score: 42.3578 bits (98), Expect = 1.462e-12
Identity = 20/61 (32.79%), Postives = 33/61 (54.10%), Query Frame = 3 
Query:  300 KLALVVRQDLKMGSGKIASQCAHAATGMYAELMQSDRHLLRKWEQCGQPKIVVTCKNQQEM 482
            K  ++VR DLK+  GK+A Q AH A     +  +      + W   GQ K+VV  ++++E+
Sbjct:    5 KQVMIVRSDLKLSKGKLAVQVAHGAVTAAFKAYKEKPEWFKGWFNEGQKKVVVKAESEREL 65
The following BLAST results are available for this feature:
BLAST of CF417557 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 42
Match NameE-valueIdentityDescription
PTH_CALMQ9.932e-1555.32Peptidyl-tRNA hydrolase OS=Caldivirga maquilingens... [more]
PTH_HALSA2.208e-1454.55Peptidyl-tRNA hydrolase OS=Halobacterium salinariu... [more]
PTH_HALS32.208e-1454.55Peptidyl-tRNA hydrolase OS=Halobacterium salinarum... [more]
PTH_THEON4.849e-1448.94Peptidyl-tRNA hydrolase OS=Thermococcus onnurineus... [more]
PTH_METKA6.303e-1439.34Peptidyl-tRNA hydrolase OS=Methanopyrus kandleri G... [more]
PTH_METTH8.191e-1438.71Peptidyl-tRNA hydrolase OS=Methanobacterium thermo... [more]
PTH2_CAEEL1.657e-1336.36Probable peptidyl-tRNA hydrolase 2 OS=Caenorhabdit... [more]
PTH_ARCFU1.125e-1246.77Peptidyl-tRNA hydrolase OS=Archaeoglobus fulgidus ... [more]
PTH_PYRAE1.442e-1249.02Peptidyl-tRNA hydrolase OS=Pyrobaculum aerophilum ... [more]
PTH_THESM1.462e-1241.38Peptidyl-tRNA hydrolase OS=Thermococcus sibiricus ... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_115000-487 Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange Citrus sinensis cDNA clone IDFL-006_F01 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF417557 ID=CF417557; Name=CF417557; organism=Citrus sinensis; type=EST; length=712bp
GTCCGTGCAACTGTTAACCACGCAATCAGTAATCTGTTCATAATCTGTTA
ACGACGATGTGGGCTTCCCAGCAAAACTCTTCTCAGCTTTCTAACAAGAA
GCAGCAGAAGCAGGAAAAAGTATCGTTAGCAGTGAGTTTTAGGCCAGAGA
ATTTCATCCCAGGCCTTGTAATTGGTTTCATTTTTGGGATGTTATTAGAT
TTATCAAAGCCAGGTAAGAATTATGTGAAAAAGAAAAATTTCTTACCGAG
GAAACCACAAGAACAAGTTTCTGTCTCAAGTGACGGTGACCAAGACCTCA
AATTGGCTTTGGTGGTTAGGCAAGACCTGAAGATGGGGAGTGGAAAGATT
GCATCACAATGTGCCCATGCAGCCACTGGGATGTATGCAGAACTAATGCA
AAGTGACCGGCACCTTCTGAGAAAATGGGAGCAATGTGGGCAGCCCAAGA
TAGTTGTTACGTGCAAGAATCAACAAGAAATGTAAGCAGGAATAAGCTGT
GGGAAGTAGCTGAAAACACGGGCCTTCCAACTTTTGTTGTTGCGGATGCT
GGACGGACACAGGTTTCAGCTGGGTCGAAGACGGTTCTTGCCATCGGACC
TGGACCCAAATCTTTGGTGGATTCTGTGACAGGGAAACAGCGCCTGCTCT
AGTTATGCTTAAGAATGGAGGACTTTTTGCTTAAATTTCCCCGGATGACA
ATGTTAAAAGAT
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