CF417915 | Citrus Genome Database

Overview


Name	CF417915
Unique Name	CF417915
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	708

Libraries


Library Name	Type
Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CF417915 vs. ExPASy Swiss-Prot
Match: TDX1_CAEEL (Probable peroxiredoxin prdx-3 OS=Caenorhabditis elegans GN=prdx-3 PE=2 SV=1)

HSP 1 Score: 129.798 bits (325), Expect = 1.629e-35
Identity = 68/134 (50.75%), Postives = 86/134 (64.18%), Query Frame = 2

Query:  200 SRKSFVVKASVEIPPL-VGNTAPDFAAEAVFDQEFINVKLSDYIGKKYVILFFYPLDFTFVCPTEITAFSDRYTEFEKLNTEILGVSTDSVFSHLAWVQTDRKSGGLGDLKYPLIADITKSISKSYGVLIPGSG 598
            +R+    +A + + PL   NT P F   AV D +F  +   DY GK ++++FFYPLDFTFVCPTEI A+ DR  EF  L  E++  S DS FSHLAWV T RK GGLGD+  PL+AD  K I+ S+GVL   SG
Sbjct:   18 TRQLSTSRALLSLRPLGPKNTVPAFKGTAVVDGDFKVISDQDYKGK-WLVMFFYPLDFTFVCPTEIIAYGDRANEFRSLGAEVVACSCDSHFSHLAWVNTPRKDGGLGDMDIPLLADFNKKIADSFGVLDKESG 150

HSP 2 Score: 38.5058 bits (88), Expect = 1.629e-35
Identity = 16/29 (55.17%), Postives = 22/29 (75.86%), Query Frame = 1

Query:  622 LLTKKGVIQHSTINNLAIGRSVDETLRTL 708
            L+   G ++H+T N+L +GRSVDETLR L
Sbjct:  158 LIDPSGTVRHTTCNDLPVGRSVDETLRVL 186

HSP 3 Score: 21.1718 bits (43), Expect = 1.629e-35
Identity = 7/13 (53.85%), Postives = 11/13 (84.62%), Query Frame = 3

Query:  591 DQGIALRGLFIID 629
            + G++ RGLF+ID
Sbjct:  148 ESGLSYRGLFLID 160

[Close]

BLAST of CF417915 vs. ExPASy Swiss-Prot
Match: PRDX1_DROME (Peroxiredoxin 1 OS=Drosophila melanogaster GN=Jafrac1 PE=1 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 2.130e-35
Identity = 66/119 (55.46%), Postives = 85/119 (71.43%), Query Frame = 2

Query:  242 PLVGNTAPDFAAEAVFDQEFINVKLSDYIGKKYVILFFYPLDFTFVCPTEITAFSDRYTEFEKLNTEILGVSTDSVFSHLAWVQTDRKSGGLGDLKYPLIADITKSISKSYGVLIPGSG 598
            P +   AP FA  AV +  F ++KLSDY GK Y++LFFYPLDFTFVCPTEI AFS+   EF K+N E++G STDS F+HLAW+ T RK GGLG +  PL+AD +  +++ YGVL   +G
Sbjct:    2 PQLQKPAPAFAGTAVVNGVFKDIKLSDYKGK-YLVLFFYPLDFTFVCPTEIIAFSESAAEFRKINCEVIGCSTDSQFTHLAWINTPRKQGGLGSMDIPLLADKSMKVARDYGVLDEETG 119

HSP 2 Score: 29.261 bits (64), Expect = 2.130e-35
Identity = 12/27 (44.44%), Postives = 20/27 (74.07%), Query Frame = 1

Query:  622 LLTKKGVIQHSTINNLAIGRSVDETLR 702
            ++  K  ++  T+N+L +GRSV+ETLR
Sbjct:  127 IIDDKQNLRQITVNDLPVGRSVEETLR 153

HSP 3 Score: 22.7126 bits (47), Expect = 2.130e-35
Identity = 9/17 (52.94%), Postives = 13/17 (76.47%), Query Frame = 3

Query:  591 DQGIALRGLFIIDKERS 641
            + GI  RGLFIID +++
Sbjct:  117 ETGIPFRGLFIIDDKQN 133

[Close]

BLAST of CF417915 vs. ExPASy Swiss-Prot
Match: TDX_ONCMY (Peroxiredoxin OS=Oncorhynchus mykiss PE=2 SV=1)

HSP 1 Score: 134.806 bits (338), Expect = 4.647e-35
Identity = 67/116 (57.76%), Postives = 81/116 (69.83%), Query Frame = 2

Query:  248 VGNTAPDFAAEAVF-DQEFINVKLSDYIGKKYVILFFYPLDFTFVCPTEITAFSDRYTEFEKLNTEILGVSTDSVFSHLAWVQTDRKSGGLGDLKYPLIADITKSISKSYGVLIPG 592
            +G+ AP F A+AV  D +F ++ +SDY GK YV+ FFYPLDFTFVCPTEI AFSD   EF K+  E++G S DS F HLAW  T RK GGLG +K PL+AD  +SIS  YGV   G
Sbjct:    8 IGHLAPGFTAKAVMPDGQFKDISMSDYRGK-YVVFFFYPLDFTFVCPTEIIAFSDAAEEFRKIGCEVIGASVDSHFCHLAWTNTPRKHGGLGAMKIPLVADTMRSISTDYGVFEGG 122

HSP 2 Score: 32.7278 bits (73), Expect = 4.647e-35
Identity = 14/27 (51.85%), Postives = 20/27 (74.07%), Query Frame = 1

Query:  622 LLTKKGVIQHSTINNLAIGRSVDETLR 702
            ++  KGV++  TIN+L +GR VDE LR
Sbjct:  133 IIDDKGVLRQITINDLPVGRCVDEILR 159

[Close]

BLAST of CF417915 vs. ExPASy Swiss-Prot
Match: TSA1_SCHPO (Peroxiredoxin tpx1 OS=Schizosaccharomyces pombe GN=tpx1 PE=1 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 1.025e-34
Identity = 61/117 (52.14%), Postives = 82/117 (70.09%), Query Frame = 2

Query:  248 VGNTAPDFAAEAVFDQEFINVKLSDYIGKKYVILFFYPLDFTFVCPTEITAFSDRYTEFEKLNTEILGVSTDSVFSHLAWVQTDRKSGGLGDLKYPLIADITKSISKSYGVLIPGSG 598
            +G  APDF   AV +  F  +KL+DY GK +V L FYPLDFTFVCPTEI AFS+  ++F + N +++  STDS +SHLA++ T RK GGLG +  PL+AD +  +S+ YGVLI  +G
Sbjct:    5 IGKPAPDFKGTAVVNGAFEEIKLADYKGK-WVFLGFYPLDFTFVCPTEIVAFSEAASKFAERNAQVILTSTDSEYSHLAFINTPRKEGGLGGINIPLLADPSHKVSRDYGVLIEDAG 120

HSP 2 Score: 36.965 bits (84), Expect = 1.025e-34
Identity = 17/29 (58.62%), Postives = 22/29 (75.86%), Query Frame = 1

Query:  622 LLTKKGVIQHSTINNLAIGRSVDETLRTL 708
            L+  KGV++  TIN+L +GRSVDE LR L
Sbjct:  128 LIDPKGVLRQITINDLPVGRSVDEALRLL 156

HSP 3 Score: 24.2534 bits (51), Expect = 1.025e-34
Identity = 9/13 (69.23%), Postives = 11/13 (84.62%), Query Frame = 3

Query:  591 DQGIALRGLFIID 629
            D G+A RGLF+ID
Sbjct:  118 DAGVAFRGLFLID 130

[Close]

BLAST of CF417915 vs. ExPASy Swiss-Prot
Match: R20K_CLOPA (Putative peroxiredoxin in rubredoxin operon OS=Clostridium pasteurianum PE=3 SV=1)

HSP 1 Score: 120.553 bits (301), Expect = 1.411e-33
Identity = 61/120 (50.83%), Postives = 77/120 (64.17%), Query Frame = 2

Query:  245 LVGNTAPDFAAEAVFD--QEFINVKLSDYIGKKYVILFFYPLDFTFVCPTEITAFSDRYTEFEKLNTEILGVSTDSVFSHLAWVQTDRKSGGLGDLKYPLIADITKSISKSYGVLIPGSG 598
            LVG  AP+F  +AV    + F  VKL DY GK ++++FFYPLDFTFVCPTEIT FS R  EF  L  E+L VS DS +SH  W+  D K GGLG + +P+ +D T  +S  YG+ I   G
Sbjct:    4 LVGKPAPEFEMKAVKGDGRGFTEVKLGDYKGK-WLVMFFYPLDFTFVCPTEITGFSKRAEEFRDLKAELLAVSCDSQYSHETWINQDIKQGGLGKINFPIASDKTTEVSTKYGIQIEEEG 122

HSP 2 Score: 35.4242 bits (80), Expect = 1.411e-33
Identity = 13/29 (44.83%), Postives = 24/29 (82.76%), Query Frame = 1

Query:  622 LLTKKGVIQHSTINNLAIGRSVDETLRTL 708
            ++  +G++++S +++L +GRSVDETLR L
Sbjct:  130 IIDPEGIVRYSVVHDLNVGRSVDETLRVL 158

HSP 3 Score: 26.9498 bits (58), Expect = 1.411e-33
Identity = 11/15 (73.33%), Postives = 14/15 (93.33%), Query Frame = 3

Query:  591 DQGIALRGLFIIDKE 635
            ++GI+LRGLFIID E
Sbjct:  120 EEGISLRGLFIIDPE 134

[Close]

BLAST of CF417915 vs. ExPASy Swiss-Prot
Match: TDX_FASHE (Thioredoxin peroxidase OS=Fasciola hepatica PE=2 SV=1)

HSP 1 Score: 128.257 bits (321), Expect = 5.412e-33
Identity = 62/114 (54.39%), Postives = 82/114 (71.93%), Query Frame = 2

Query:  260 APDFAAEAVFDQEFINVKLSDYIGKKYVILFFYPLDFTFVCPTEITAFSDRYTEFEKLNTEILGVSTDSVFSHLAWVQTDRKSGGLGDLKYPLIADITKSISKSYGVLIPGSGN 601
            AP+F+ +AV  +EF  + LSDY GK +VIL FYPLDFTFVCPTEI A SD+  +F + N  ++  STDSV+SHL W + DRK GG+G L +PL+AD   S+S+++GVL    GN
Sbjct:    8 APNFSGQAVVGKEFETISLSDYKGK-WVILAFYPLDFTFVCPTEIIAISDQMEQFAQRNCAVIFCSTDSVYSHLQWTKMDRKVGGIGQLNFPLLADKNMSVSRAFGVLDEEQGN 120

HSP 2 Score: 32.3426 bits (72), Expect = 5.412e-33
Identity = 14/31 (45.16%), Postives = 22/31 (70.97%), Query Frame = 1

Query:  616 SLLLTKKGVIQHSTINNLAIGRSVDETLRTL 708
            + L+  KGV++  T+N+  +GRSV+E LR L
Sbjct:  125 NFLIDPKGVLRQITVNDDPVGRSVEEALRLL 155

[Close]

BLAST of CF417915 vs. ExPASy Swiss-Prot
Match: TSA1_YEAST (Peroxiredoxin TSA1 OS=Saccharomyces cerevisiae GN=TSA1 PE=1 SV=3)

HSP 1 Score: 110.923 bits (276), Expect = 3.420e-31
Identity = 60/117 (51.28%), Postives = 71/117 (60.68%), Query Frame = 2

Query:  248 VGNTAPDFAAEAVFDQEFINVKLSDYIGKKYVILFFYPLDFTFVCPTEITAFSDRYTEFEKLNTEILGVSTDSVFSHLAWVQTDRKSGGLGDLKYPLIADITKSISKSYGVLIPGSG 598
            V   AP F   AV D  F  V L  Y GK YV+L F PL FTFVCPTEI AFS+   +FE+   ++L  STDS +S LAW    RK GGLG +  PL+AD   S+S+ YGVLI   G
Sbjct:    5 VQKQAPTFKKTAVVDGVFDEVSLDKYKGK-YVVLAFIPLAFTFVCPTEIIAFSEAAKKFEEQGAQVLFASTDSEYSLLAWTNIPRKEGGLGPINIPLLADTNHSLSRDYGVLIEEEG 120

HSP 2 Score: 37.7354 bits (86), Expect = 3.420e-31
Identity = 16/27 (59.26%), Postives = 22/27 (81.48%), Query Frame = 1

Query:  622 LLTKKGVIQHSTINNLAIGRSVDETLR 702
            ++  KGVI+H TIN+L +GR+VDE LR
Sbjct:  128 IIDPKGVIRHITINDLPVGRNVDEALR 154

HSP 3 Score: 26.1794 bits (56), Expect = 3.420e-31
Identity = 10/13 (76.92%), Postives = 13/13 (100.00%), Query Frame = 3

Query:  591 DQGIALRGLFIID 629
            ++G+ALRGLFIID
Sbjct:  118 EEGVALRGLFIID 130

[Close]

BLAST of CF417915 vs. ExPASy Swiss-Prot
Match: PRDX2_PIG (Peroxiredoxin-2 (Fragment) OS=Sus scrofa GN=PRDX2 PE=2 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 4.567e-31
Identity = 54/84 (64.29%), Postives = 63/84 (75.00%), Query Frame = 2

Query:  347 LFFYPLDFTFVCPTEITAFSDRYTEFEKLNTEILGVSTDSVFSHLAWVQTDRKSGGLGDLKYPLIADITKSISKSYGVLIPGSG 598
            LFFYPLDFTFVCPTEI AFSDR  EF +L  E+LGVS D   +HLAW+ T RK GGLG LK PL+AD+T+++S  YGVL    G
Sbjct:    1 LFFYPLDFTFVCPTEIIAFSDRAEEFHQLGCEVLGVSVDXQXTHLAWINTPRKEGGLGPLKIPLLADVTRNLSLDYGVLKEDEG 84

HSP 2 Score: 32.3426 bits (72), Expect = 4.567e-31
Identity = 13/27 (48.15%), Postives = 20/27 (74.07%), Query Frame = 1

Query:  622 LLTKKGVIQHSTINNLAIGRSVDETLR 702
            ++  KGV++  T+N+L +GR VDE LR
Sbjct:   92 IIDGKGVLRQITVNDLPVGRXVDEALR 118

HSP 3 Score: 26.1794 bits (56), Expect = 4.567e-31
Identity = 11/13 (84.62%), Postives = 12/13 (92.31%), Query Frame = 3

Query:  591 DQGIALRGLFIID 629
            D+GIA RGLFIID
Sbjct:   82 DEGIAYRGLFIID 94

[Close]

BLAST of CF417915 vs. ExPASy Swiss-Prot
Match: TDX_TRYBR (Thioredoxin peroxidase OS=Trypanosoma brucei rhodesiense PE=2 SV=1)

HSP 1 Score: 119.398 bits (298), Expect = 9.725e-31
Identity = 61/113 (53.98%), Postives = 76/113 (67.26%), Query Frame = 2

Query:  248 VGNTAPDFAAEAVFDQ-EFINVKLSDYIGKKYVILFFYPLDFTFVCPTEITAFSDRYTEFEKLNTEILGVSTDSVFSHLAWVQTDRKSGGLGDLKYPLIADITKSISKSYGVL 583
            + + AP F   A+     F  V L+ Y GK +V+LFFYPLDFTFVCPTEI  FSDR  EF  ++ E++  S DS FSHLAW   +RK GGLG +  P++AD TKSI K+YGVL
Sbjct:    8 LNHPAPHFNEVALMPNGTFKKVDLASYRGK-WVVLFFYPLDFTFVCPTEICQFSDRVKEFNDVDCEVIACSMDSEFSHLAWTNVERKKGGLGTMNIPILADKTKSIMKAYGVL 119

HSP 2 Score: 29.261 bits (64), Expect = 9.725e-31
Identity = 12/20 (60.00%), Postives = 17/20 (85.00%), Query Frame = 1

Query:  643 IQHSTINNLAIGRSVDETLR 702
            ++  TIN+L +GR+VDETLR
Sbjct:  139 LRQITINDLPVGRNVDETLR 158

HSP 3 Score: 24.6386 bits (52), Expect = 9.725e-31
Identity = 9/17 (52.94%), Postives = 14/17 (82.35%), Query Frame = 3

Query:  591 DQGIALRGLFIIDKERS 641
            + G+A RGLFIID +++
Sbjct:  122 EDGVAYRGLFIIDPQQN 138

[Close]

BLAST of CF417915 vs. ExPASy Swiss-Prot
Match: TSA2_YEAST (Peroxiredoxin TSA2 OS=Saccharomyces cerevisiae GN=TSA2 PE=1 SV=3)

HSP 1 Score: 110.538 bits (275), Expect = 1.641e-30
Identity = 60/117 (51.28%), Postives = 71/117 (60.68%), Query Frame = 2

Query:  248 VGNTAPDFAAEAVFDQEFINVKLSDYIGKKYVILFFYPLDFTFVCPTEITAFSDRYTEFEKLNTEILGVSTDSVFSHLAWVQTDRKSGGLGDLKYPLIADITKSISKSYGVLIPGSG 598
            V   AP F   AV D  F  + L  Y GK YV+L F PL F+FVCPTEI AFSD   +FE    ++L  STDS +S LAW    RK GGLG +K PL+AD   S+S+ YGVLI   G
Sbjct:    5 VQKQAPPFKKTAVVDGIFEEISLEKYKGK-YVVLAFVPLAFSFVCPTEIVAFSDAAKKFEDQGAQVLFASTDSEYSLLAWTNLPRKDGGLGPVKVPLLADKNHSLSRDYGVLIEKEG 120

HSP 2 Score: 36.1946 bits (82), Expect = 1.641e-30
Identity = 14/27 (51.85%), Postives = 23/27 (85.19%), Query Frame = 1

Query:  622 LLTKKGVIQHSTINNLAIGRSVDETLR 702
            ++  KG+I+H TIN+L++GR+V+E LR
Sbjct:  128 IIDPKGIIRHITINDLSVGRNVNEALR 154

HSP 3 Score: 25.7942 bits (55), Expect = 1.641e-30
Identity = 11/12 (91.67%), Postives = 12/12 (100.00%), Query Frame = 3

Query:  594 QGIALRGLFIID 629
            +GIALRGLFIID
Sbjct:  119 EGIALRGLFIID 130

The following BLAST results are available for this feature:

BLAST of CF417915 vs. ExPASy Swiss-Prot

BLAST of CF417915 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 127


Match Name	E-value	Identity	Description
TDX1_CAEEL	1.629e-35	50.75	Probable peroxiredoxin prdx-3 OS=Caenorhabditis el...	[more]
PRDX1_DROME	2.130e-35	55.46	Peroxiredoxin 1 OS=Drosophila melanogaster GN=Jafr...	[more]
TDX_ONCMY	4.647e-35	57.76	Peroxiredoxin OS=Oncorhynchus mykiss PE=2 SV=1	[more]
TSA1_SCHPO	1.025e-34	52.14	Peroxiredoxin tpx1 OS=Schizosaccharomyces pombe GN...	[more]
R20K_CLOPA	1.411e-33	50.83	Putative peroxiredoxin in rubredoxin operon OS=Clo...	[more]
TDX_FASHE	5.412e-33	54.39	Thioredoxin peroxidase OS=Fasciola hepatica PE=2 S...	[more]
TSA1_YEAST	3.420e-31	51.28	Peroxiredoxin TSA1 OS=Saccharomyces cerevisiae GN=...	[more]
PRDX2_PIG	4.567e-31	64.29	Peroxiredoxin-2 (Fragment) OS=Sus scrofa GN=PRDX2 ...	[more]
TDX_TRYBR	9.725e-31	53.98	Thioredoxin peroxidase OS=Trypanosoma brucei rhode...	[more]
TSA2_YEAST	1.641e-30	51.28	Peroxiredoxin TSA2 OS=Saccharomyces cerevisiae GN=...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_115000-845 Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange Citrus sinensis cDNA clone IDFL-011_E12 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF417915 ID=CF417915; Name=CF417915; organism=Citrus sinensis; type=EST; length=708bp
AACAAAAACAATGGCTTGCTCAGCTGCTTCTGCTGCTGCCACGGCAACAG
CCGCTCTCATTTCCTCTAACCCCAAGGCTTTCTCTTCTTCCTCAATCTCC
CCTTCTCAAAACCTAACCATTCCCCGCTCCTTCTCCGGCCTCCGCAATCC
CCTCCAGTCCCAGGTCCCCCGCCCCGTTTCTCTTTCCCGCGGGTCCCGTT
CTCGCAAGTCCTTCGTTGTCAAAGCATCCGTTGAAATACCACCATTGGTG
GGCAACACGGCACCTGATTTTGCTGCAGAGGCTGTTTTCGATCAAGAGTT
CATCAATGTCAAGCTTTCGGATTACATTGGGAAGAAATATGTGATTCTCT
TTTTCTACCCGTTGGACTTCACATTTGTTTGCCCCACAGAAATCACTGCT
TTCAGCGACCGATATACTGAATTTGAGAAGCTAAACACAGAGATATTGGG
TGTCTCAACCGACAGTGTGTTTTCACACTTGGCATGGGTCCAAACAGACA
GAAAGTCAGGTGGTCTTGGTGATTTGAAGTACCCACTAATTGCTGATATC
ACCAAATCAATTTCAAAATCCTATGGTGTTCTGATCCCTGGATCAGGGAA
TTGCATTGAGAGGACTCTTTATTATTGACAAAGAAAGGAGTCATCCAGCA
CTCAACCATTAACAACCTTGCCATTGGTAGGAGTGTTGACGAAACACTGA
GAACACTT