CF503860 | Citrus Genome Database

Overview


Name	CF503860
Unique Name	CF503860
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	333

Libraries


Library Name	Type
Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

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BLAST of CF503860 vs. ExPASy Swiss-Prot
Match: FAL1_PICST (ATP-dependent RNA helicase FAL1 OS=Pichia stipitis GN=FAL1 PE=3 SV=1)

HSP 1 Score: 167.548 bits (423), Expect = 1.834e-41
Identity = 76/110 (69.09%), Postives = 96/110 (87.27%), Query Frame = 3

Query:    3 RVCDMIKRKTLRTRAIKLLVLDESDEMLSRGFKDQIYDVYRYLPPDLQVVLISATLPHEILEMTTKFMTDPVKILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTL 332
            RV DM+KR+ L TR IK+L+LDE+DE+ ++GFK+QIY+VY+YLPP +QVV++SATL  E+LEMT KF TDPVKILVKRD++TLEGIKQ+ V  E+E+WKFDTLCDLYD L
Sbjct:  154 RVVDMVKRQQLSTRNIKMLILDEADELFTKGFKEQIYEVYKYLPPSVQVVVVSATLSREVLEMTNKFTTDPVKILVKRDQITLEGIKQYHVQCEKEDWKFDTLCDLYDNL 263

[Close]

BLAST of CF503860 vs. ExPASy Swiss-Prot
Match: FAL1_DEBHA (ATP-dependent RNA helicase FAL1 OS=Debaryomyces hansenii GN=FAL1 PE=3 SV=1)

HSP 1 Score: 166.777 bits (421), Expect = 3.128e-41
Identity = 74/110 (67.27%), Postives = 98/110 (89.09%), Query Frame = 3

Query:    3 RVCDMIKRKTLRTRAIKLLVLDESDEMLSRGFKDQIYDVYRYLPPDLQVVLISATLPHEILEMTTKFMTDPVKILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTL 332
            RV DMIKR+ L TR IK+++LDE+DE++++GFK+QIY++YRYLPP +QVV++SATL  E+LE+T KF TDPVKILVKRD++TLEGIKQ+ +  E+E+WKFDTLCDLYD+L
Sbjct:  154 RVVDMIKRRNLATRNIKMMILDEADELMTKGFKEQIYEIYRYLPPGVQVVVVSATLSREVLEVTGKFTTDPVKILVKRDDITLEGIKQYHIQCEKEDWKFDTLCDLYDSL 263

[Close]

BLAST of CF503860 vs. ExPASy Swiss-Prot
Match: FAL1_LODEL (ATP-dependent RNA helicase FAL1 OS=Lodderomyces elongisporus GN=FAL1 PE=3 SV=1)

HSP 1 Score: 163.696 bits (413), Expect = 2.648e-40
Identity = 72/110 (65.45%), Postives = 97/110 (88.18%), Query Frame = 3

Query:    3 RVCDMIKRKTLRTRAIKLLVLDESDEMLSRGFKDQIYDVYRYLPPDLQVVLISATLPHEILEMTTKFMTDPVKILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTL 332
            RV D+IKR+ L TR IK+L+LDE+DE+ ++GFK+QIY++Y++LPP +QVV++SATL HE+LEMT KF TDPVKILVKR+E++L GIKQ+++  E+E+WKFDTLCDLYD L
Sbjct:  154 RVLDVIKRRNLSTRHIKMLILDEADELFTKGFKEQIYEIYKHLPPGVQVVVVSATLTHEVLEMTGKFTTDPVKILVKREEVSLSGIKQYYIQCEKEDWKFDTLCDLYDNL 263

[Close]

BLAST of CF503860 vs. ExPASy Swiss-Prot
Match: IF4A_USTMA (ATP-dependent RNA helicase eIF4A OS=Ustilago maydis GN=TIF1 PE=3 SV=1)

HSP 1 Score: 161.77 bits (408), Expect = 1.006e-39
Identity = 74/110 (67.27%), Postives = 94/110 (85.45%), Query Frame = 3

Query:    3 RVCDMIKRKTLRTRAIKLLVLDESDEMLSRGFKDQIYDVYRYLPPDLQVVLISATLPHEILEMTTKFMTDPVKILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTL 332
            RV DMI R+  +T  +K+  LDE+DEMLSRGFKDQ+Y+V++ LP D Q VL+SAT+P E+LE+T KFM DP++ILVKRDELTLEGIKQF+VAVE+E+WK DTLCDLY+T+
Sbjct:  166 RVYDMINRRAFKTDQLKMFCLDEADEMLSRGFKDQMYEVFQLLPQDTQCVLLSATMPQEVLEVTKKFMRDPIRILVKRDELTLEGIKQFYVAVEKEDWKLDTLCDLYETV 275

[Close]

BLAST of CF503860 vs. ExPASy Swiss-Prot
Match: IF4A1_ARATH (Eukaryotic initiation factor 4A-1 OS=Arabidopsis thaliana GN=TIF4A-1 PE=1 SV=1)

HSP 1 Score: 161.77 bits (408), Expect = 1.006e-39
Identity = 77/110 (70.00%), Postives = 95/110 (86.36%), Query Frame = 3

Query:    3 RVCDMIKRKTLRTRAIKLLVLDESDEMLSRGFKDQIYDVYRYLPPDLQVVLISATLPHEILEMTTKFMTDPVKILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTL 332
            RV DM+KR++LR   IK+ VLDE+DEMLSRGFKDQIYD+++ LPP +QV + SAT+P E LE+T KFM+ PV+ILVKRDELTLEGIKQF+V VE+EEWK +TLCDLY+TL
Sbjct:  167 RVFDMLKRQSLRADNIKMFVLDEADEMLSRGFKDQIYDIFQLLPPKIQVGVFSATMPPEALEITRKFMSKPVRILVKRDELTLEGIKQFYVNVEKEEWKLETLCDLYETL 276

[Close]

BLAST of CF503860 vs. ExPASy Swiss-Prot
Match: IF4A_DROME (Eukaryotic initiation factor 4A OS=Drosophila melanogaster GN=eIF-4a PE=2 SV=3)

HSP 1 Score: 160.614 bits (405), Expect = 2.241e-39
Identity = 77/110 (70.00%), Postives = 94/110 (85.45%), Query Frame = 3

Query:    3 RVCDMIKRKTLRTRAIKLLVLDESDEMLSRGFKDQIYDVYRYLPPDLQVVLISATLPHEILEMTTKFMTDPVKILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTL 332
            RV DMI RK LRT+ IKL VLDE+DEMLSRGFKDQI DV++ LPPD+QV+L+SAT+P ++LE++  FM DPV ILVK++ELTLEGIKQF+V V++E WK  TLCDLYDTL
Sbjct:  158 RVYDMINRKVLRTQYIKLFVLDEADEMLSRGFKDQIQDVFKMLPPDVQVILLSATMPPDVLEVSRCFMRDPVSILVKKEELTLEGIKQFYVNVKQENWKLGTLCDLYDTL 267

[Close]

BLAST of CF503860 vs. ExPASy Swiss-Prot
Match: IF4A3_ORYSJ (Eukaryotic initiation factor 4A-3 OS=Oryza sativa subsp. japonica GN=Os02g0146600 PE=2 SV=1)

HSP 1 Score: 160.229 bits (404), Expect = 2.927e-39
Identity = 76/110 (69.09%), Postives = 94/110 (85.45%), Query Frame = 3

Query:    3 RVCDMIKRKTLRTRAIKLLVLDESDEMLSRGFKDQIYDVYRYLPPDLQVVLISATLPHEILEMTTKFMTDPVKILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTL 332
            RV DM++R++LR   IK+ VLDE+DEMLSRGFKDQIYD+++ LPP +QV + SAT+P E LE+T KFM  PV+ILVKRDELTLEGIKQF+V VE+E+WK DTLCDLY+TL
Sbjct:  169 RVFDMLRRQSLRPDHIKMFVLDEADEMLSRGFKDQIYDIFQLLPPKIQVGVFSATMPPEALEITRKFMNKPVRILVKRDELTLEGIKQFYVNVEKEDWKLDTLCDLYETL 278

[Close]

BLAST of CF503860 vs. ExPASy Swiss-Prot
Match: IF4A_MAGGR (ATP-dependent RNA helicase eIF4A OS=Magnaporthe grisea GN=TIF1 PE=3 SV=1)

HSP 1 Score: 159.844 bits (403), Expect = 3.823e-39
Identity = 74/110 (67.27%), Postives = 96/110 (87.27%), Query Frame = 3

Query:    3 RVCDMIKRKTLRTRAIKLLVLDESDEMLSRGFKDQIYDVYRYLPPDLQVVLISATLPHEILEMTTKFMTDPVKILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTL 332
            RV DMI+R+ L+T  +K+ VLDE+DEMLSRGF +QIYD+++ LP   QVVL+SAT+P ++LE+TTKFM DPV+ILVK+DELTLEGIKQF++AVE+EEWK DTL DLY+T+
Sbjct:  151 RVHDMIQRRFLKTDGMKMFVLDEADEMLSRGFTEQIYDIFQLLPQSTQVVLLSATMPQDVLEVTTKFMRDPVRILVKKDELTLEGIKQFYIAVEKEEWKLDTLSDLYETV 260

[Close]

BLAST of CF503860 vs. ExPASy Swiss-Prot
Match: IF4A2_ARATH (Eukaryotic initiation factor 4A-2 OS=Arabidopsis thaliana GN=TIF4A-2 PE=1 SV=1)

HSP 1 Score: 159.844 bits (403), Expect = 3.823e-39
Identity = 75/110 (68.18%), Postives = 95/110 (86.36%), Query Frame = 3

Query:    3 RVCDMIKRKTLRTRAIKLLVLDESDEMLSRGFKDQIYDVYRYLPPDLQVVLISATLPHEILEMTTKFMTDPVKILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTL 332
            RV DM++R++LR   IK+ VLDE+DEMLSRGFKDQIYD+++ LPP +QV + SAT+P E LE+T KFM+ PV+ILVKRDELTLEGIKQF+V VE+E+WK +TLCDLY+TL
Sbjct:  167 RVFDMLRRQSLRPDCIKMFVLDEADEMLSRGFKDQIYDIFQLLPPKIQVGVFSATMPPEALEITRKFMSKPVRILVKRDELTLEGIKQFYVNVEKEDWKLETLCDLYETL 276

[Close]

BLAST of CF503860 vs. ExPASy Swiss-Prot
Match: IF4A_ASPCL (ATP-dependent RNA helicase eIF4A OS=Aspergillus clavatus GN=tif1 PE=3 SV=1)

HSP 1 Score: 159.458 bits (402), Expect = 4.993e-39
Identity = 74/110 (67.27%), Postives = 95/110 (86.36%), Query Frame = 3

Query:    3 RVCDMIKRKTLRTRAIKLLVLDESDEMLSRGFKDQIYDVYRYLPPDLQVVLISATLPHEILEMTTKFMTDPVKILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTL 332
            RV DMI+R+ LRT  +KL +LDE+DEMLSRGF +QIYD+++ LP   QVVL+SAT+P ++LE+TTKFM DPV+ILVK+ ELTLEGIKQF++AVE+EEWK DTL DLY+T+
Sbjct:  153 RVQDMIQRRVLRTDQMKLFILDEADEMLSRGFTEQIYDIFQLLPQSTQVVLLSATMPQDVLEVTTKFMRDPVRILVKKQELTLEGIKQFYIAVEKEEWKLDTLSDLYETV 262

The following BLAST results are available for this feature:

BLAST of CF503860 vs. ExPASy Swiss-Prot

BLAST of CF503860 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 238


Match Name	E-value	Identity	Description
FAL1_PICST	1.834e-41	69.09	ATP-dependent RNA helicase FAL1 OS=Pichia stipitis...	[more]
FAL1_DEBHA	3.128e-41	67.27	ATP-dependent RNA helicase FAL1 OS=Debaryomyces ha...	[more]
FAL1_LODEL	2.648e-40	65.45	ATP-dependent RNA helicase FAL1 OS=Lodderomyces el...	[more]
IF4A_USTMA	1.006e-39	67.27	ATP-dependent RNA helicase eIF4A OS=Ustilago maydi...	[more]
IF4A1_ARATH	1.006e-39	70.00	Eukaryotic initiation factor 4A-1 OS=Arabidopsis t...	[more]
IF4A_DROME	2.241e-39	70.00	Eukaryotic initiation factor 4A OS=Drosophila mela...	[more]
IF4A3_ORYSJ	2.927e-39	69.09	Eukaryotic initiation factor 4A-3 OS=Oryza sativa ...	[more]
IF4A_MAGGR	3.823e-39	67.27	ATP-dependent RNA helicase eIF4A OS=Magnaporthe gr...	[more]
IF4A2_ARATH	3.823e-39	68.18	Eukaryotic initiation factor 4A-2 OS=Arabidopsis t...	[more]
IF4A_ASPCL	4.993e-39	67.27	ATP-dependent RNA helicase eIF4A OS=Aspergillus cl...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_119000-347 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-10_F02 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF503860 ID=CF503860; Name=CF503860; organism=Citrus sinensis; type=EST; length=333bp
GTAGAGTCTGTGATATGATTAAGAGGAAAACTTTGCGTACTAGAGCTATT
AAATTATTGGTGCTTGATGAGTCTGATGAGATGCTGAGCAGAGGGTTCAA
GGATCAAATTTATGATGTGTACCGTTATCTTCCACCGGATCTTCAGGTTG
TTTTGATATCTGCCACCCTTCCTCACGAAATATTGGAGATGACGACCAAA
TTCATGACAGATCCTGTAAAGATTCTTGTGAAGCGTGATGAGTTGACTTT
GGAGGGAATCAAGCAATTTTTTGTTGCAGTGGAAAGAGAGGAGTGGAAGT
TTGATACACTCTGTGATCTTTATGATACCCTTA