CF503882

Overview
NameCF503882
Unique NameCF503882
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length356
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig2013 contig Csv1_Contig2013:196..547. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF503882 vs. ExPASy Swiss-Prot
Match: PIP26_ARATH (Probable aquaporin PIP2-6 OS=Arabidopsis thaliana GN=PIP2-6 PE=1 SV=1)

HSP 1 Score: 110.923 bits (276), Expect = 6.461e-34
Identity = 52/67 (77.61%), Postives = 57/67 (85.07%), Query Frame = 1 
Query:  154 IGYKSQTDPNLNTDQCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLGRKVSLIRAL 354
            IG+KSQTD N     C  VG+LGI+WAFGGMIFILVYCTAGISGGHINPAVTFGLFL  KVSL+RA+
Sbjct:   59 IGFKSQTDINAGGGACASVGLLGISWAFGGMIFILVYCTAGISGGHINPAVTFGLFLASKVSLVRAV 125

HSP 2 Score: 52.7582 bits (125), Expect = 6.461e-34
Identity = 24/34 (70.59%), Postives = 25/34 (73.53%), Query Frame = 3 
Query:   15 SAKDYHDPPPAPLIDFEELGKWSFYRAVIAEFIA 116
            S KDY DPPP    +  EL KWSFYRAVIAEFIA
Sbjct:   13 SGKDYLDPPPVKTFEVRELKKWSFYRAVIAEFIA 46
[Close]
BLAST of CF503882 vs. ExPASy Swiss-Prot
Match: PIP1_ATRCA (Aquaporin PIP-type OS=Atriplex canescens PE=2 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 5.353e-33
Identity = 52/67 (77.61%), Postives = 57/67 (85.07%), Query Frame = 1 
Query:  154 IGYKSQTDPNLNTDQCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLGRKVSLIRAL 354
            IGYK +TDP      C  VG+LGIAW+FGGMIF+LVYCTAGISGGHINPAVTFGLFL RKVSL+RAL
Sbjct:   61 IGYKKETDP------CASVGLLGIAWSFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLLRAL 121

HSP 2 Score: 51.2174 bits (121), Expect = 5.353e-33
Identity = 23/32 (71.88%), Postives = 24/32 (75.00%), Query Frame = 3 
Query:   21 KDYHDPPPAPLIDFEELGKWSFYRAVIAEFIA 116
            KDY DPPPAP  D  EL  WSF+RA IAEFIA
Sbjct:   17 KDYVDPPPAPFFDMGELKLWSFWRAAIAEFIA 48
[Close]
BLAST of CF503882 vs. ExPASy Swiss-Prot
Match: PIP27_ORYSJ (Probable aquaporin PIP2-7 OS=Oryza sativa subsp. japonica GN=PIP2-7 PE=2 SV=2)

HSP 1 Score: 95.1301 bits (235), Expect = 1.785e-30
Identity = 43/67 (64.18%), Postives = 51/67 (76.12%), Query Frame = 1 
Query:  154 IGYKSQTDPNLNTDQCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLGRKVSLIRAL 354
            IGYK+Q       D C GVG LG+AW+FG  IF+LVYCT G+SGGHINPAVT GLF GRK+SL+R +
Sbjct:   65 IGYKNQ---RATVDACTGVGYLGVAWSFGATIFVLVYCTGGVSGGHINPAVTLGLFFGRKLSLVRTV 128

HSP 2 Score: 56.9954 bits (136), Expect = 1.785e-30
Identity = 29/48 (60.42%), Postives = 36/48 (75.00%), Query Frame = 3 
Query:    3 GGEFSAK-DYHDPPPAPLIDFEELGKWSFYRAVIAEFIA---YAFVSL 134
            GG  +AK  Y DPPPAPL+D  ELGKWS YRA+IAEF+A   + +VS+
Sbjct:   14 GGAAAAKAPYWDPPPAPLLDTSELGKWSLYRALIAEFMATLIFLYVSI 61
[Close]
BLAST of CF503882 vs. ExPASy Swiss-Prot
Match: PIP15_MAIZE (Aquaporin PIP1-5 OS=Zea mays GN=PIP1-5 PE=2 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 2.501e-29
Identity = 44/56 (78.57%), Postives = 49/56 (87.50%), Query Frame = 1 
Query:  187 NTDQCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLGRKVSLIRAL 354
            ++ +C  VGI GIAW+FGGMIF LVYCTAGISGGHINPAVTFGLFL RK+SL RAL
Sbjct:   80 SSSKCATVGIQGIAWSFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRAL 135

HSP 2 Score: 53.5286 bits (127), Expect = 2.501e-29
Identity = 23/32 (71.88%), Postives = 26/32 (81.25%), Query Frame = 3 
Query:   21 KDYHDPPPAPLIDFEELGKWSFYRAVIAEFIA 116
            KDY +PPPAPL + EEL  WSFYRA IAEF+A
Sbjct:   31 KDYKEPPPAPLFEAEELTSWSFYRAGIAEFVA 62
[Close]
BLAST of CF503882 vs. ExPASy Swiss-Prot
Match: PIP12_MAIZE (Aquaporin PIP1-2 OS=Zea mays GN=PIP1-2 PE=1 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 7.186e-29
Identity = 45/56 (80.36%), Postives = 49/56 (87.50%), Query Frame = 1 
Query:  187 NTDQCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLGRKVSLIRAL 354
            +T +C  VGI GIAW+FGGMIF LVYCTAGISGGHINPAVTFGLFL RK+SL RAL
Sbjct:   81 STSKCATVGIQGIAWSFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRAL 136

HSP 2 Score: 50.447 bits (119), Expect = 7.186e-29
Identity = 23/37 (62.16%), Postives = 26/37 (70.27%), Query Frame = 3 
Query:    6 GEFSAKDYHDPPPAPLIDFEELGKWSFYRAVIAEFIA 116
            G    KDY +PPPAPL +  EL  WSFYRA IAEF+A
Sbjct:   27 GAADDKDYKEPPPAPLFEPGELKSWSFYRAGIAEFVA 63
[Close]
BLAST of CF503882 vs. ExPASy Swiss-Prot
Match: PIP12_ORYSJ (Probable aquaporin PIP1-2 OS=Oryza sativa subsp. japonica GN=PIP1-2 PE=2 SV=3)

HSP 1 Score: 97.4413 bits (241), Expect = 7.188e-29
Identity = 46/58 (79.31%), Postives = 50/58 (86.21%), Query Frame = 1 
Query:  181 NLNTDQCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLGRKVSLIRAL 354
            N +T +C  VGI GIAW+FGGMIF LVYCTAGISGGHINPAVTFGLFL RK+SL RAL
Sbjct:   78 NNSTSKCATVGIQGIAWSFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRAL 135

HSP 2 Score: 49.2914 bits (116), Expect = 7.188e-29
Identity = 22/32 (68.75%), Postives = 25/32 (78.12%), Query Frame = 3 
Query:   21 KDYHDPPPAPLIDFEELGKWSFYRAVIAEFIA 116
            KDY +PPPAPL +  EL  WSFYRA IAEF+A
Sbjct:   31 KDYKEPPPAPLFEPGELKSWSFYRAGIAEFMA 62
[Close]
BLAST of CF503882 vs. ExPASy Swiss-Prot
Match: PIP28_ORYSJ (Probable aquaporin PIP2-8 OS=Oryza sativa subsp. japonica GN=PIP2-8 PE=2 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 7.191e-29
Identity = 39/50 (78.00%), Postives = 45/50 (90.00%), Query Frame = 1 
Query:  202 GGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLGRKVSLIRA 351
            GG G+LGIAWAFGG+IF+LVYCTAGISGGH+NPAVTF + L R+VSL RA
Sbjct:   65 GGAGVLGIAWAFGGLIFVLVYCTAGISGGHMNPAVTFAMVLARRVSLPRA 114

HSP 2 Score: 58.5362 bits (140), Expect = 7.191e-29
Identity = 26/38 (68.42%), Postives = 28/38 (73.68%), Query Frame = 3 
Query:    3 GGEFSAKDYHDPPPAPLIDFEELGKWSFYRAVIAEFIA 116
            G   + KDY DPPPAPL+D  ELGKWS YRA IAEF A
Sbjct:    6 GSGSNPKDYQDPPPAPLVDTGELGKWSLYRAAIAEFTA 43
[Close]
BLAST of CF503882 vs. ExPASy Swiss-Prot
Match: PIP14_ARATH (Probable aquaporin PIP1-4 OS=Arabidopsis thaliana GN=PIP1.4 PE=1 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 1.218e-28
Identity = 44/54 (81.48%), Postives = 47/54 (87.04%), Query Frame = 1 
Query:  193 DQCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLGRKVSLIRAL 354
            + C  VGI GIAWAFGGMIF LVYCTAGISGGHINPAVTFGLFL RK+SL RA+
Sbjct:   81 NMCASVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRAV 134

HSP 2 Score: 51.2174 bits (121), Expect = 1.218e-28
Identity = 23/32 (71.88%), Postives = 25/32 (78.12%), Query Frame = 3 
Query:   21 KDYHDPPPAPLIDFEELGKWSFYRAVIAEFIA 116
            KDY +PPPAPL +  EL  WSFYRA IAEFIA
Sbjct:   30 KDYKEPPPAPLFEPGELSSWSFYRAGIAEFIA 61
[Close]
BLAST of CF503882 vs. ExPASy Swiss-Prot
Match: PIP13_MAIZE (Aquaporin PIP1-3/PIP1-4 OS=Zea mays GN=PIP1-3 PE=2 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 1.586e-28
Identity = 44/56 (78.57%), Postives = 49/56 (87.50%), Query Frame = 1 
Query:  187 NTDQCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLGRKVSLIRAL 354
            +T +C  VGI GIAW+FGGMIF LVYCTAGISGGHINPAVTFGLFL RK+SL RA+
Sbjct:   84 STSKCATVGIQGIAWSFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRAI 139

HSP 2 Score: 50.0618 bits (118), Expect = 1.586e-28
Identity = 22/32 (68.75%), Postives = 25/32 (78.12%), Query Frame = 3 
Query:   21 KDYHDPPPAPLIDFEELGKWSFYRAVIAEFIA 116
            KDY +PPPAPL +  EL  WSFYRA IAEF+A
Sbjct:   35 KDYKEPPPAPLFEPGELKSWSFYRAGIAEFVA 66
[Close]
BLAST of CF503882 vs. ExPASy Swiss-Prot
Match: PIP2_PEA (Probable aquaporin PIP-type 7a OS=Pisum sativum GN=TRG-31 PE=2 SV=2)

HSP 1 Score: 93.9745 bits (232), Expect = 1.586e-28
Identity = 44/55 (80.00%), Postives = 48/55 (87.27%), Query Frame = 1 
Query:  190 TDQCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLGRKVSLIRAL 354
            + +C  VGI GIAWAFGGMIF LVYCTAGISGGHINPAVTFGLFL RK+SL RA+
Sbjct:   82 SSKCKTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRAI 136

HSP 2 Score: 51.6026 bits (122), Expect = 1.586e-28
Identity = 23/32 (71.88%), Postives = 25/32 (78.12%), Query Frame = 3 
Query:   21 KDYHDPPPAPLIDFEELGKWSFYRAVIAEFIA 116
            KDY +PPPAPL +  EL  WSFYRA IAEFIA
Sbjct:   32 KDYQEPPPAPLFEPSELTSWSFYRAGIAEFIA 63
The following BLAST results are available for this feature:
BLAST of CF503882 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 40
Match NameE-valueIdentityDescription
PIP26_ARATH6.461e-3477.61Probable aquaporin PIP2-6 OS=Arabidopsis thaliana ... [more]
PIP1_ATRCA5.353e-3377.61Aquaporin PIP-type OS=Atriplex canescens PE=2 SV=1[more]
PIP27_ORYSJ1.785e-3064.18Probable aquaporin PIP2-7 OS=Oryza sativa subsp. j... [more]
PIP15_MAIZE2.501e-2978.57Aquaporin PIP1-5 OS=Zea mays GN=PIP1-5 PE=2 SV=1[more]
PIP12_MAIZE7.186e-2980.36Aquaporin PIP1-2 OS=Zea mays GN=PIP1-2 PE=1 SV=1[more]
PIP12_ORYSJ7.188e-2979.31Probable aquaporin PIP1-2 OS=Oryza sativa subsp. j... [more]
PIP28_ORYSJ7.191e-2978.00Probable aquaporin PIP2-8 OS=Oryza sativa subsp. j... [more]
PIP14_ARATH1.218e-2881.48Probable aquaporin PIP1-4 OS=Arabidopsis thaliana ... [more]
PIP13_MAIZE1.586e-2878.57Aquaporin PIP1-3/PIP1-4 OS=Zea mays GN=PIP1-3 PE=2... [more]
PIP2_PEA1.586e-2880.00Probable aquaporin PIP-type 7a OS=Pisum sativum GN... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_119000-369 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-10_H03 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF503882 ID=CF503882; Name=CF503882; organism=Citrus sinensis; type=EST; length=356bp
GCGGCGGCGAGTTCTCAGCCAAGGACTACCACGACCCCCCTCCGGCGCCG
CTGATCGATTTCGAGGAGCTGGGCAAGTGGTCTTTTTACAGAGCTGTAAT
TGCTGAGTTCATCGCCTACGCTTTTGTTTCTTTATGTTACCGTCTTGACT
GTGATTGGATACAAGAGCCAGACTGATCCCAACTTGAATACCGATCAGTG
TGGTGGCGTTGGCATTCTTGGCATCGCTTGGGCCTTTGGTGGCATGATCT
TCATTCTCGTTTACTGCACAGCCGGTATTTCTGGTGGACATATCAACCCG
GCTGTGACATTCGGACTGTTCTTGGGGCGCAAGGTGTCGCTCATCCGAGC
TCTTTT
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