CF503965 | Citrus Genome Database

Overview


Name	CF503965
Unique Name	CF503965
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	639

Libraries


Library Name	Type
Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
CF503965_ssr7	microsatellite	CF503965_ssr7:7..28.	BLAST: Citrus ESTs to Prunus persica proteins V1

Homology

[Close]

BLAST of CF503965 vs. ExPASy Swiss-Prot
Match: ORYA_ORYSJ (Oryzain alpha chain OS=Oryza sativa subsp. japonica GN=Os04g0650000 PE=1 SV=2)

HSP 1 Score: 75.0998 bits (183), Expect = 1.088e-25
Identity = 33/44 (75.00%), Postives = 37/44 (84.09%), Query Frame = 3

Query:  507 EDLPQSVDWRKKGAVTHVKNQNPCGGCWAFSAVAAVEGINQIVT 638
            E LP+SVDWR KGAV  +K+Q  CG CWAFSA+AAVEGINQIVT
Sbjct:  127 EALPESVDWRTKGAVAEIKDQGGCGSCWAFSAIAAVEGINQIVT 170

HSP 2 Score: 62.003 bits (149), Expect = 1.088e-25
Identity = 32/87 (36.78%), Postives = 50/87 (57.47%), Query Frame = 1

Query:  145 EFSIVGYSLEVLTSTDKLVELFESWMLKHGKSYESTEEKLHRLEIFKDNLKHIDARNRELQI-TSSYWLGLNEFSDMSHEEFKNKYL 402
            + SIV Y      S ++   L+  W  +HGKSY +  E+  R   F+DNL++ID  N        S+ LGLN F+D+++EE+++ YL
Sbjct:   22 DMSIVSYGER---SEEEARRLYAEWKAEHGKSYNAVGEEERRYAAFRDNLRYIDEHNAAADAGVHSFRLGLNRFADLTNEEYRDTYL 105

[Close]

BLAST of CF503965 vs. ExPASy Swiss-Prot
Match: CPR3_ARATH (Probable cysteine proteinase At4g11310 OS=Arabidopsis thaliana GN=At4g11310 PE=2 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 3.358e-24
Identity = 29/42 (69.05%), Postives = 34/42 (80.95%), Query Frame = 3

Query:  513 LPQSVDWRKKGAVTHVKNQNPCGGCWAFSAVAAVEGINQIVT 638
            LP+SVDWR +GAVT VK+Q  C  CWAFS V AVEG+N+IVT
Sbjct:  137 LPKSVDWRNEGAVTEVKDQGHCRSCWAFSTVGAVEGLNKIVT 178

HSP 2 Score: 65.4698 bits (158), Expect = 3.358e-24
Identity = 34/62 (54.84%), Postives = 42/62 (67.74%), Query Frame = 1

Query:  205 LFESWMLKHGKSYESTEEKLHRLEIFKDNLKHIDARNRELQITSSYWLGLNEFSDMSHEEFK 390
            +FESWM+KHGK Y S  EK  RL IF+DNL+ I+ RN E     SY LGL  F+D+S  E+K
Sbjct:   48 IFESWMVKHGKVYGSVAEKERRLTIFEDNLRFINNRNAE---NLSYRLGLTGFADLSLHEYK 106

[Close]

BLAST of CF503965 vs. ExPASy Swiss-Prot
Match: CPR2_ARATH (Probable cysteine proteinase At3g43960 OS=Arabidopsis thaliana GN=At3g43960 PE=2 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 1.252e-23
Identity = 35/91 (38.46%), Postives = 58/91 (63.74%), Query Frame = 1

Query:  154 IVGYSLEVLTSTD------KLVELFESWMLKHGKSYESTEEKLHRLEIFKDNLKHIDARNRELQITSSYWLGLNEFSDMSHEEFKNKYLTG 408
            ++  SL V+T+T+      +++ ++E W++++GK+Y    EK  R +IFKDNLK I+  N +     SY  GLN+FSD++ +EF+  YL G
Sbjct:   17 LISISLGVVTATESQRNEGEVLTMYEQWLVENGKNYNGLGEKERRFKIFKDNLKRIEEHNSD--PNRSYERGLNKFSDLTADEFQASYLGG 105

HSP 2 Score: 61.6178 bits (148), Expect = 1.252e-23
Identity = 28/43 (65.12%), Postives = 31/43 (72.09%), Query Frame = 3

Query:  513 LPQSVDWRKKGAVT-HVKNQNPCGGCWAFSAVAAVEGINQIVT 638
            LP  VDWR++GAV   VK Q  CG CWAF+A  AVEGINQI T
Sbjct:  127 LPDEVDWRERGAVVPRVKRQGECGSCWAFAATGAVEGINQITT 169

[Close]

BLAST of CF503965 vs. ExPASy Swiss-Prot
Match: ANAN_ANACO (Ananain OS=Ananas comosus GN=AN1 PE=1 SV=2)

HSP 1 Score: 66.2402 bits (160), Expect = 1.632e-23
Identity = 31/75 (41.33%), Postives = 53/75 (70.67%), Query Frame = 1

Query:  187 TDKLVELFESWMLKHGKSYESTEEKLHRLEIFKDNLKHIDARNRELQITSSYWLGLNEFSDMSHEEFKNKYLTGL 411
            +D +++ FE WM ++G+ Y+  +EK+ R +IFK+N+ HI+  N   +  +SY LG+N+F+DM++ EF  +Y TGL
Sbjct:   30 SDPMMKQFEEWMAEYGRVYKDNDEKMLRFQIFKNNVNHIETFNN--RNGNSYTLGINQFTDMTNNEFVAQY-TGL 101

HSP 2 Score: 63.5438 bits (153), Expect = 1.632e-23
Identity = 27/49 (55.10%), Postives = 35/49 (71.43%), Query Frame = 3

Query:  486 SFFVQNVEDLPQSVDWRKKGAVTHVKNQNPCGGCWAFSAVAAVEGINQI 632
            SF   ++  +PQS+DWR  GAVT VKNQ  CG CWAF+++A VE I +I
Sbjct:  114 SFDDVDISSVPQSIDWRDSGAVTSVKNQGRCGSCWAFASIATVESIYKI 162

[Close]

BLAST of CF503965 vs. ExPASy Swiss-Prot
Match: CYSEP_RICCO (Vignain OS=Ricinus communis GN=CYSEP PE=1 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 4.996e-22
Identity = 33/51 (64.71%), Postives = 39/51 (76.47%), Query Frame = 3

Query:  486 SFFVQNVEDLPQSVDWRKKGAVTHVKNQNPCGGCWAFSAVAAVEGINQIVT 638
            +F  + V+ +P SVDWRKKGAVT VK+Q  CG CWAFS + AVEGINQI T
Sbjct:  117 TFMYEKVDTVPASVDWRKKGAVTSVKDQGQCGSCWAFSTIVAVEGINQIKT 167

HSP 2 Score: 48.9062 bits (115), Expect = 4.996e-22
Identity = 28/74 (37.84%), Postives = 39/74 (52.70%), Query Frame = 1

Query:  178 LTSTDKLVELFESWMLKHGKSYESTEEKLHRLEIFKDNLKHIDARNRELQITSSYWLGLNEFSDMSHEEFKNKY 399
            L S + L  L+E W   H  S  S  EK  R  +FK N  H+   N+   +   Y L LN+F+DM++ EF+N Y
Sbjct:   28 LESEESLWGLYERWRSHHTVS-RSLHEKQKRFNVFKHNAMHVHNANK---MDKPYKLKLNKFADMTNHEFRNTY 97

[Close]

BLAST of CF503965 vs. ExPASy Swiss-Prot
Match: CYSP_HEMSP (Thiol protease SEN102 OS=Hemerocallis sp. GN=SEN102 PE=2 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 4.996e-22
Identity = 34/52 (65.38%), Postives = 39/52 (75.00%), Query Frame = 3

Query:  486 SFFVQNVEDLPQ-SVDWRKKGAVTHVKNQNPCGGCWAFSAVAAVEGINQIVT 638
            SF  +NV  LP  S+DWR KGAVT VK+Q  CG CWAFS +A+VEGINQI T
Sbjct:  120 SFMYENVGSLPAASIDWRAKGAVTGVKDQGQCGSCWAFSTIASVEGINQIKT 171

HSP 2 Score: 52.7582 bits (125), Expect = 4.996e-22
Identity = 28/74 (37.84%), Postives = 44/74 (59.46%), Query Frame = 1

Query:  178 LTSTDKLVELFESWMLKHGKSYESTEEKLHRLEIFKDNLKHIDARNRELQITSSYWLGLNEFSDMSHEEFKNKY 399
            L S D L  L+E W   H  + +  +EK  R  +FK+N+K I   N++    + Y L LN+F DM+++EF++KY
Sbjct:   30 LASEDSLWNLYEKWRTHHTVARD-LDEKNRRFNVFKENVKFIHEFNQKKD--APYKLALNKFGDMTNQEFRSKY 100

[Close]

BLAST of CF503965 vs. ExPASy Swiss-Prot
Match: CYSEP_PHAVU (Vignain OS=Phaseolus vulgaris PE=2 SV=2)

HSP 1 Score: 75.0998 bits (183), Expect = 6.499e-22
Identity = 34/51 (66.67%), Postives = 38/51 (74.51%), Query Frame = 3

Query:  486 SFFVQNVEDLPQSVDWRKKGAVTHVKNQNPCGGCWAFSAVAAVEGINQIVT 638
            +F  + V  +P SVDWRKKGAVT VK+Q  CG CWAFS V AVEGINQI T
Sbjct:  119 AFMYEKVVSVPPSVDWRKKGAVTDVKDQGQCGSCWAFSTVVAVEGINQIKT 169

HSP 2 Score: 49.2914 bits (116), Expect = 6.499e-22
Identity = 28/74 (37.84%), Postives = 41/74 (55.41%), Query Frame = 1

Query:  178 LTSTDKLVELFESWMLKHGKSYESTEEKLHRLEIFKDNLKHIDARNRELQITSSYWLGLNEFSDMSHEEFKNKY 399
            L S + L +L+E W   H  S  S  EK  R  +FK NL H+   N+   +   Y L LN+F+DM++ EF++ Y
Sbjct:   30 LASEESLWDLYERWRSHHTVS-RSLGEKHKRFNVFKANLMHVHNTNK---MDKPYKLKLNKFADMTNHEFRSTY 99

[Close]

BLAST of CF503965 vs. ExPASy Swiss-Prot
Match: CYSEP_VIGMU (Vignain OS=Vigna mungo PE=1 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 2.422e-21
Identity = 33/51 (64.71%), Postives = 38/51 (74.51%), Query Frame = 3

Query:  486 SFFVQNVEDLPQSVDWRKKGAVTHVKNQNPCGGCWAFSAVAAVEGINQIVT 638
            +F  + V  +P SVDWRKKGAVT VK+Q  CG CWAFS + AVEGINQI T
Sbjct:  119 TFMYEKVGSVPASVDWRKKGAVTDVKDQGQCGSCWAFSTIVAVEGINQIKT 169

HSP 2 Score: 47.7506 bits (112), Expect = 2.422e-21
Identity = 27/74 (36.49%), Postives = 41/74 (55.41%), Query Frame = 1

Query:  178 LTSTDKLVELFESWMLKHGKSYESTEEKLHRLEIFKDNLKHIDARNRELQITSSYWLGLNEFSDMSHEEFKNKY 399
            L S + L +L+E W   H  S  S  EK  R  +FK N+ H+   N+   +   Y L LN+F+DM++ EF++ Y
Sbjct:   30 LESEESLWDLYERWRSHHTVS-RSLGEKHKRFNVFKANVMHVHNTNK---MDKPYKLKLNKFADMTNHEFRSTY 99

[Close]

BLAST of CF503965 vs. ExPASy Swiss-Prot
Match: CPR1_ARATH (Probable cysteine proteinase At3g19400 OS=Arabidopsis thaliana GN=At3g19400 PE=2 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 2.422e-21
Identity = 30/42 (71.43%), Postives = 31/42 (73.81%), Query Frame = 3

Query:  513 LPQSVDWRKKGAVTHVKNQNPCGGCWAFSAVAAVEGINQIVT 638
            LP  VDWR  GAV  VK+Q  CG CWAFSAV AVEGINQI T
Sbjct:  130 LPDEVDWRANGAVVSVKDQGNCGSCWAFSAVGAVEGINQITT 171

HSP 2 Score: 55.4546 bits (132), Expect = 2.422e-21
Identity = 31/89 (34.83%), Postives = 52/89 (58.43%), Query Frame = 1

Query:  154 IVGYSLEVLTSTD------KLVELFESWMLKHGKSYESTEEKLHRLEIFKDNLKHIDARNRELQITSSYWLGLNEFSDMSHEEFKNKYL 402
            ++  SL V T T+      ++  ++E W++++ K+Y    EK  R +IFKDNLK +D  N     T  + +GL  F+D+++EEF+  YL
Sbjct:   20 LLSSSLGVATETEIERNETEVRLMYEQWLVENRKNYNGLGEKERRFKIFKDNLKFVDEHNSVPDRT--FEVGLTRFADLTNEEFRAIYL 106

[Close]

BLAST of CF503965 vs. ExPASy Swiss-Prot
Match: P34_SOYBN (P34 probable thiol protease OS=Glycine max PE=1 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 1.172e-20
Identity = 38/101 (37.62%), Postives = 58/101 (57.43%), Query Frame = 1

Query:  112 SSSRTLQGHDGEFSIVGYSLEVLTSTDKLVELFESWMLKHGKSYESTEEKLHRLEIFKDNLKHIDARNRELQITSSYWLGLNEFSDMSHEEFKNKYLTGLK 414
            SSS ++  H    SI+   L   T+  ++  LF+ W  +HG+ Y + EE+  RLEIFK+N  +I   N   +   S+ LGLN+F+D++ +EF  KYL   K
Sbjct:   15 SSSSSISTHR---SILDLDLTKFTTQKQVSSLFQLWKSEHGRVYHNHEEEAKRLEIFKNNSNYIRDMNANRKSPHSHRLGLNKFADITPQEFSKKYLQAPK 112

HSP 2 Score: 51.6026 bits (122), Expect = 1.172e-20
Identity = 23/41 (56.10%), Postives = 26/41 (63.41%), Query Frame = 3

Query:  516 PQSVDWRKKGAVTHVKNQNPCGGCWAFSAVAAVEGINQIVT 638
            P S DWRKKG +T VK Q  CG  WAFSA  A+E  + I T
Sbjct:  136 PASWDWRKKGVITQVKYQGGCGRGWAFSATGAIEAAHAIAT 176

The following BLAST results are available for this feature:

BLAST of CF503965 vs. ExPASy Swiss-Prot

BLAST of CF503965 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 125


Match Name	E-value	Identity	Description
ORYA_ORYSJ	1.088e-25	75.00	Oryzain alpha chain OS=Oryza sativa subsp. japonic...	[more]
CPR3_ARATH	3.358e-24	69.05	Probable cysteine proteinase At4g11310 OS=Arabidop...	[more]
CPR2_ARATH	1.252e-23	38.46	Probable cysteine proteinase At3g43960 OS=Arabidop...	[more]
ANAN_ANACO	1.632e-23	41.33	Ananain OS=Ananas comosus GN=AN1 PE=1 SV=2	[more]
CYSEP_RICCO	4.996e-22	64.71	Vignain OS=Ricinus communis GN=CYSEP PE=1 SV=1	[more]
CYSP_HEMSP	4.996e-22	65.38	Thiol protease SEN102 OS=Hemerocallis sp. GN=SEN10...	[more]
CYSEP_PHAVU	6.499e-22	66.67	Vignain OS=Phaseolus vulgaris PE=2 SV=2	[more]
CYSEP_VIGMU	2.422e-21	64.71	Vignain OS=Vigna mungo PE=1 SV=1	[more]
CPR1_ARATH	2.422e-21	71.43	Probable cysteine proteinase At3g19400 OS=Arabidop...	[more]
P34_SOYBN	1.172e-20	37.62	P34 probable thiol protease OS=Glycine max PE=1 SV...	[more]

Pages

Properties


Property Name	Value
Genbank description	USDA-FP_119000-453 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-11_G08 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF503965 ID=CF503965; Name=CF503965; organism=Citrus sinensis; type=EST; length=639bp
GTGGGGGAGAGAGAGAGAGAGAGAGAGATGAAAATGTTATCTTCCAATTG
TAAGTTGTTGCTTCGTCTTTCTTTGTGCGTGTCTCTCTTGATGATCATAT
GCATACGCTGCTCTAGCAGTAGAACTCTTCAAGGTCATGATGGTGAGTTC
TCCATTGTGGGGTATTCACTAGAGGTGTTGACATCCACGGACAAGCTGGT
TGAGCTATTCGAGTCATGGATGTTGAAACATGGGAAATCGTATGAAAGCA
CGGAAGAGAAGTTGCATAGATTGGAGATATTCAAAGACAACTTGAAGCAC
ATTGATGCGAGGAACAGAGAGCTGCAAATTACTAGTAGCTATTGGCTTGG
TCTGAATGAGTTTTCAGATATGAGCCACGAAGAGTTCAAGAACAAGTACT
TAACAGGACTCAAACCCGATGATGATGAGTTCCGCAGAAGGAGTCACCGC
GACTCCAGTAGTAGATCTTCTGCTAGCAGTAGTAATCATTTTTTGTACAA
AATGTAGAGGATTTGCCTCAATCCGTTGACTGGAGGAAGAAAGGAGCTGT
CACTCATGTCAAAAACCAAAATCCTTGTGGTGGATGTTGGGCATTTTCAG
CAGTAGCAGCAGTGGAGGGGATAAATCAGATAGTGACGG