CF503965

Overview
NameCF503965
Unique NameCF503965
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length639
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CF503965 vs. ExPASy Swiss-Prot
Match: CYSP4_BRANA (Cysteine proteinase COT44 (Fragment) OS=Brassica napus PE=2 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 1.176e-20
Identity = 30/46 (65.22%), Postives = 38/46 (82.61%), Query Frame = 3 
Query:  501 NVEDLPQSVDWRKKGAVTHVKNQNPCGGCWAFSAVAAVEGINQIVT 638
            NV+++P +VDWR+KGAV  +K+Q  CG CWAFS  AAVEGIN+IVT
Sbjct:   96 NVDEVPVTVDWRQKGAVNAIKDQGTCGSCWAFSTAAAVEGINKIVT 141

HSP 2 Score: 48.521 bits (114), Expect = 1.176e-20
Identity = 25/72 (34.72%), Postives = 45/72 (62.50%), Query Frame = 1 
Query:  199 VELFESWMLKHGKSYESTE----EKLHRLEIFKDNLKHIDARNRELQITSSYWLGLNEFSDMSHEEFKNKYL 402
            + ++  W L+HGKS  ++     ++  R  IFKDNL+ ID  N E    ++Y LGL  F++++++E+++ YL
Sbjct:    1 MSIYLRWSLEHGKSNSNSNGIINQQDERFNIFKDNLRFIDLHN-ENNKNATYKLGLTIFANLTNDEYRSLYL 71
[Close]
BLAST of CF503965 vs. ExPASy Swiss-Prot
Match: ORYB_ORYSJ (Oryzain beta chain OS=Oryza sativa subsp. japonica GN=Os04g0670200 PE=1 SV=2)

HSP 1 Score: 75.485 bits (184), Expect = 1.518e-20
Identity = 33/45 (73.33%), Postives = 38/45 (84.44%), Query Frame = 3 
Query:  504 VEDLPQSVDWRKKGAVTHVKNQNPCGGCWAFSAVAAVEGINQIVT 638
            VE+LP+SVDWR+KGAV  VKNQ  CG CWAFSAV+ VE INQ+VT
Sbjct:  138 VEELPESVDWREKGAVAPVKNQGQCGSCWAFSAVSTVESINQLVT 182

HSP 2 Score: 44.2838 bits (103), Expect = 1.518e-20
Identity = 21/67 (31.34%), Postives = 37/67 (55.22%), Query Frame = 1 
Query:  208 FESWMLKHGKSYEST--EEKLHRLEIFKDNLKHIDARNRELQITSSYWLGLNEFSDMSHEEFKNKYL 402
            ++ W+ ++G    +    E   R  +F DNLK +DA N        + LG+N F+D+++EEF+  +L
Sbjct:   52 YDLWLAENGGGSPNALGGEHERRFLVFWDNLKFVDAHNARADERGGFRLGMNRFADLTNEEFRATFL 118
[Close]
BLAST of CF503965 vs. ExPASy Swiss-Prot
Match: GCP1_ARATH (Germination-specific cysteine protease 1 OS=Arabidopsis thaliana GN=GCP1 PE=2 SV=2)

HSP 1 Score: 70.0922 bits (170), Expect = 1.984e-20
Identity = 29/46 (63.04%), Postives = 38/46 (82.61%), Query Frame = 3 
Query:  501 NVEDLPQSVDWRKKGAVTHVKNQNPCGGCWAFSAVAAVEGINQIVT 638
            N +++P++VDWR+KGAV  +K+Q  CG CWAFS  AAVEGIN+IVT
Sbjct:  141 NGKEVPETVDWRQKGAVNPIKDQGTCGSCWAFSTTAAVEGINKIVT 186

HSP 2 Score: 49.2914 bits (116), Expect = 1.984e-20
Identity = 30/96 (31.25%), Postives = 56/96 (58.33%), Query Frame = 1 
Query:  142 GEFSIVGYSLEVLTS----TDKLVE-LFESWMLKHGKSYESTEEKLH----RLEIFKDNLKHIDARNRELQITSSYWLGLNEFSDMSHEEFKNKYL 402
            G+ SI+   L++ +     TD+ V  ++  W  +HGK+  +    ++    R  IFKDNL+ ID  N + +  ++Y LGL +F+D++++E++  YL
Sbjct:   22 GDESIINDHLQLPSDGKWRTDEEVRSIYLQWSAEHGKTNNNNNGIINDQDKRFNIFKDNLRFIDLHNEDNK-NATYKLGLTKFTDLTNDEYRKLYL 116
[Close]
BLAST of CF503965 vs. ExPASy Swiss-Prot
Match: CYSP1_ORYSJ (Cysteine protease 1 OS=Oryza sativa subsp. japonica GN=CP1 PE=2 SV=2)

HSP 1 Score: 72.0182 bits (175), Expect = 1.615e-19
Identity = 36/46 (78.26%), Postives = 37/46 (80.43%), Query Frame = 3 
Query:  504 VEDLPQSVDWRKKGAVTH-VKNQNPCGGCWAFSAVAAVEGINQIVT 638
            VE LP SVDWR KGAV   VKNQ  CG CWAFSAVAAVEGIN+IVT
Sbjct:  152 VEALPDSVDWRDKGAVVAPVKNQGQCGSCWAFSAVAAVEGINKIVT 197

HSP 2 Score: 44.2838 bits (103), Expect = 1.615e-19
Identity = 22/71 (30.99%), Postives = 35/71 (49.30%), Query Frame = 1 
Query:  208 FESWMLKH------GKSYESTEEKLHRLEIFKDNLKHIDARNRELQITSSYWLGLNEFSDMSHEEFKNKYL 402
            ++ W+ +H      G       E   R  +F DNLK +DA N        + LG+N F+D+++ EF+  YL
Sbjct:   62 YDLWLARHRRGGGGGSRNGFIGEHERRFRVFWDNLKFVDAHNARADERGGFRLGMNRFADLTNGEFRATYL 132
[Close]
BLAST of CF503965 vs. ExPASy Swiss-Prot
Match: CATH_BOVIN (Cathepsin H OS=Bos taurus GN=CTSH PE=2 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 2.118e-19
Identity = 32/65 (49.23%), Postives = 44/65 (67.69%), Query Frame = 1 
Query:  208 FESWMLKHGKSYESTEEKLHRLEIFKDNLKHIDARNRELQITSSYWLGLNEFSDMSHEEFKNKYL 402
            F+SWM++H K Y S+EE  HRL+ F  NL+ I+A N       ++ +GLN+FSDMS +E K KYL
Sbjct:   35 FQSWMVQHQKKY-SSEEYYHRLQAFASNLREINAHNAR---NHTFKMGLNQFSDMSFDELKRKYL 95

HSP 2 Score: 51.6026 bits (122), Expect = 2.118e-19
Identity = 25/52 (48.08%), Postives = 30/52 (57.69%), Query Frame = 3 
Query:  486 SFFVQNVEDLPQSVDWRKKGA-VTHVKNQNPCGGCWAFSAVAAVEGINQIVT 638
            S +++     P S+DWRKKG  VT VKNQ  CG CW FS   A+E    I T
Sbjct:  107 SNYLRGTGPYPPSMDWRKKGNFVTPVKNQGSCGSCWTFSTTGALESAVAIAT 158
[Close]
BLAST of CF503965 vs. ExPASy Swiss-Prot
Match: CYSP2_HORVU (Cysteine proteinase EP-B 2 OS=Hordeum vulgare GN=EPB2 PE=1 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 4.941e-18
Identity = 33/46 (71.74%), Postives = 36/46 (78.26%), Query Frame = 3 
Query:  501 NVEDLPQSVDWRKKGAVTHVKNQNPCGGCWAFSAVAAVEGINQIVT 638
            NV DLP SVDWR+KGAVT VK+Q  CG CWAFS V +VEGIN I T
Sbjct:  130 NVSDLPPSVDWRQKGAVTGVKDQGKCGSCWAFSTVVSVEGINAIRT 175

HSP 2 Score: 38.5058 bits (88), Expect = 4.941e-18
Identity = 26/81 (32.10%), Postives = 36/81 (44.44%), Query Frame = 1 
Query:  178 LTSTDKLVELFESWMLKHGKSYESTEEKLHRLEIFKDNLKHIDARNRELQITSSYWLGLNEFSDMSHEEFKNKYLTGLKPD 420
            L S + L +L+E W   H +      EK  R   FK N   I + N+       Y L LN F DM   EF+  ++  L+ D
Sbjct:   36 LESEEALWDLYERWQSAH-RVRRHHAEKHRRFGTFKSNAHFIHSHNKRGD--HPYRLHLNRFGDMDQAEFRATFVGDLRRD 113
[Close]
BLAST of CF503965 vs. ExPASy Swiss-Prot
Match: CYSP1_HORVU (Cysteine proteinase EP-B 1 OS=Hordeum vulgare GN=EPB1 PE=2 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 4.941e-18
Identity = 33/46 (71.74%), Postives = 36/46 (78.26%), Query Frame = 3 
Query:  501 NVEDLPQSVDWRKKGAVTHVKNQNPCGGCWAFSAVAAVEGINQIVT 638
            NV DLP SVDWR+KGAVT VK+Q  CG CWAFS V +VEGIN I T
Sbjct:  130 NVSDLPPSVDWRQKGAVTGVKDQGKCGSCWAFSTVVSVEGINAIRT 175

HSP 2 Score: 38.5058 bits (88), Expect = 4.941e-18
Identity = 26/81 (32.10%), Postives = 36/81 (44.44%), Query Frame = 1 
Query:  178 LTSTDKLVELFESWMLKHGKSYESTEEKLHRLEIFKDNLKHIDARNRELQITSSYWLGLNEFSDMSHEEFKNKYLTGLKPD 420
            L S + L +L+E W   H +      EK  R   FK N   I + N+       Y L LN F DM   EF+  ++  L+ D
Sbjct:   36 LESEEALWDLYERWQSAH-RVRRHHAEKHRRFGTFKSNAHFIHSHNKRGD--HPYRLHLNRFGDMDQAEFRATFVGDLRRD 113
[Close]
BLAST of CF503965 vs. ExPASy Swiss-Prot
Match: CATH_HUMAN (Cathepsin H OS=Homo sapiens GN=CTSH PE=1 SV=4)

HSP 1 Score: 59.3066 bits (142), Expect = 8.373e-18
Identity = 32/65 (49.23%), Postives = 42/65 (64.62%), Query Frame = 1 
Query:  208 FESWMLKHGKSYESTEEKLHRLEIFKDNLKHIDARNRELQITSSYWLGLNEFSDMSHEEFKNKYL 402
            F+SWM KH K+Y STEE  HRL+ F  N + I+A N       ++ + LN+FSDMS  E K+KYL
Sbjct:   35 FKSWMSKHRKTY-STEEYHHRLQTFASNWRKINAHNNG---NHTFKMALNQFSDMSFAEIKHKYL 95

HSP 2 Score: 51.2174 bits (121), Expect = 8.373e-18
Identity = 25/52 (48.08%), Postives = 30/52 (57.69%), Query Frame = 3 
Query:  486 SFFVQNVEDLPQSVDWRKKGA-VTHVKNQNPCGGCWAFSAVAAVEGINQIVT 638
            S +++     P SVDWRKKG  V+ VKNQ  CG CW FS   A+E    I T
Sbjct:  107 SNYLRGTGPYPPSVDWRKKGNFVSPVKNQGACGSCWTFSTTGALESAIAIAT 158
[Close]
BLAST of CF503965 vs. ExPASy Swiss-Prot
Match: CPR1_DROME (Putative cysteine proteinase CG12163 OS=Drosophila melanogaster GN=CG12163 PE=2 SV=2)

HSP 1 Score: 57.7658 bits (138), Expect = 1.074e-17
Identity = 23/43 (53.49%), Postives = 29/43 (67.44%), Query Frame = 3 
Query:  510 DLPQSVDWRKKGAVTHVKNQNPCGGCWAFSAVAAVEGINQIVT 638
            +LP+  DWR+K AVT VKNQ  CG CWAFS    +EG+  + T
Sbjct:  393 ELPKEFDWRQKDAVTQVKNQGSCGSCWAFSVTGNIEGLYAVKT 435

HSP 2 Score: 52.373 bits (124), Expect = 1.074e-17
Identity = 30/79 (37.97%), Postives = 46/79 (58.23%), Query Frame = 1 
Query:  190 DKLVELFESWMLKHGKSYESTEEKLHRLEIFKDNLKHIDARNRELQITSSYWLGLNEFSDMSHEEFKNKYLTGLKPDDD 426
            DK+  LF  + ++ G+ Y ST E+  RL IF+ NLK I+  N     ++ Y  G+ EF+DM+  E+K +  TGL   D+
Sbjct:  302 DKVDHLFYKFQVRFGRRYVSTAERQMRLRIFRQNLKTIEELNANEMGSAKY--GITEFADMTSSEYKER--TGLWQRDE 376
[Close]
BLAST of CF503965 vs. ExPASy Swiss-Prot
Match: CATH_PIG (Cathepsin H OS=Sus scrofa GN=CTSH PE=1 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 1.089e-17
Identity = 36/81 (44.44%), Postives = 52/81 (64.20%), Query Frame = 1 
Query:  160 GYSLEVLTSTDKLVELFESWMLKHGKSYESTEEKLHRLEIFKDNLKHIDARNRELQITSSYWLGLNEFSDMSHEEFKNKYL 402
            G S   ++S +KL   F+SWM++H K Y S EE  HRL++F  N + I+A N       ++ LGLN+FSDMS +E ++KYL
Sbjct:   21 GASNLAVSSFEKLH--FKSWMVQHQKKY-SLEEYHHRLQVFVSNWRKINAHNAG---NHTFKLGLNQFSDMSFDEIRHKYL 95

HSP 2 Score: 49.6766 bits (117), Expect = 1.089e-17
Identity = 23/50 (46.00%), Postives = 29/50 (58.00%), Query Frame = 3 
Query:  492 FVQNVEDLPQSVDWRKKGA-VTHVKNQNPCGGCWAFSAVAAVEGINQIVT 638
            +++     P S+DWRKKG  V+ VKNQ  CG CW FS   A+E    I T
Sbjct:  109 YLRGTGPYPPSMDWRKKGNFVSPVKNQGSCGSCWTFSTTGALESAVAIAT 158
The following BLAST results are available for this feature:
BLAST of CF503965 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 125
Match NameE-valueIdentityDescription
CYSP4_BRANA1.176e-2065.22Cysteine proteinase COT44 (Fragment) OS=Brassica n... [more]
ORYB_ORYSJ1.518e-2073.33Oryzain beta chain OS=Oryza sativa subsp. japonica... [more]
GCP1_ARATH1.984e-2063.04Germination-specific cysteine protease 1 OS=Arabid... [more]
CYSP1_ORYSJ1.615e-1978.26Cysteine protease 1 OS=Oryza sativa subsp. japonic... [more]
CATH_BOVIN2.118e-1949.23Cathepsin H OS=Bos taurus GN=CTSH PE=2 SV=1[more]
CYSP2_HORVU4.941e-1871.74Cysteine proteinase EP-B 2 OS=Hordeum vulgare GN=E... [more]
CYSP1_HORVU4.941e-1871.74Cysteine proteinase EP-B 1 OS=Hordeum vulgare GN=E... [more]
CATH_HUMAN8.373e-1849.23Cathepsin H OS=Homo sapiens GN=CTSH PE=1 SV=4[more]
CPR1_DROME1.074e-1753.49Putative cysteine proteinase CG12163 OS=Drosophila... [more]
CATH_PIG1.089e-1744.44Cathepsin H OS=Sus scrofa GN=CTSH PE=1 SV=1[more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_119000-453 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-11_G08 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF503965 ID=CF503965; Name=CF503965; organism=Citrus sinensis; type=EST; length=639bp
GTGGGGGAGAGAGAGAGAGAGAGAGAGATGAAAATGTTATCTTCCAATTG
TAAGTTGTTGCTTCGTCTTTCTTTGTGCGTGTCTCTCTTGATGATCATAT
GCATACGCTGCTCTAGCAGTAGAACTCTTCAAGGTCATGATGGTGAGTTC
TCCATTGTGGGGTATTCACTAGAGGTGTTGACATCCACGGACAAGCTGGT
TGAGCTATTCGAGTCATGGATGTTGAAACATGGGAAATCGTATGAAAGCA
CGGAAGAGAAGTTGCATAGATTGGAGATATTCAAAGACAACTTGAAGCAC
ATTGATGCGAGGAACAGAGAGCTGCAAATTACTAGTAGCTATTGGCTTGG
TCTGAATGAGTTTTCAGATATGAGCCACGAAGAGTTCAAGAACAAGTACT
TAACAGGACTCAAACCCGATGATGATGAGTTCCGCAGAAGGAGTCACCGC
GACTCCAGTAGTAGATCTTCTGCTAGCAGTAGTAATCATTTTTTGTACAA
AATGTAGAGGATTTGCCTCAATCCGTTGACTGGAGGAAGAAAGGAGCTGT
CACTCATGTCAAAAACCAAAATCCTTGTGGTGGATGTTGGGCATTTTCAG
CAGTAGCAGCAGTGGAGGGGATAAATCAGATAGTGACGG
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