CF503965 | Citrus Genome Database

Overview


Name	CF503965
Unique Name	CF503965
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	639

Libraries


Library Name	Type
Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
CF503965_ssr7	microsatellite	CF503965_ssr7:7..28.	BLAST: Citrus ESTs to Prunus persica proteins V1

Homology

[Close]

BLAST of CF503965 vs. ExPASy Swiss-Prot
Match: CATLL_FASHE (Cathepsin L-like proteinase OS=Fasciola hepatica GN=Cat-1 PE=1 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 1.089e-17
Identity = 34/94 (36.17%), Postives = 52/94 (55.32%), Query Frame = 1

Query:  148 FSIVGYSLEVLTSTDKLVELFESWMLKHGKSYESTEEKLHRLEIFKDNLKHIDARNRELQI-TSSYWLGLNEFSDMSHEEFKNKYLTGLKPDDD 426
            F +   ++ VL S D   +L+  W   + K Y   +++ HR  I++ N+KHI   N    +   +Y LGLN+F+DM+ EEFK KYLT +    D
Sbjct:    4 FILAVLTVGVLGSND---DLWHQWKRMYNKEYNGADDQ-HRRNIWEKNVKHIQEHNLRHDLGLVTYTLGLNQFTDMTFEEFKAKYLTEMSRASD 93

HSP 2 Score: 51.6026 bits (122), Expect = 1.089e-17
Identity = 20/40 (50.00%), Postives = 25/40 (62.50%), Query Frame = 3

Query:  501 NVEDLPQSVDWRKKGAVTHVKNQNPCGGCWAFSAVAAVEG 620
            N   +P  +DWR+ G VT VK+Q  CG CWAFS    +EG
Sbjct:  104 NNRAVPDKIDWRESGYVTEVKDQGNCGSCWAFSTTGTMEG 143

[Close]

BLAST of CF503965 vs. ExPASy Swiss-Prot
Match: CATH_RAT (Cathepsin H OS=Rattus norvegicus GN=Ctsh PE=1 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 2.393e-17
Identity = 31/65 (47.69%), Postives = 42/65 (64.62%), Query Frame = 1

Query:  208 FESWMLKHGKSYESTEEKLHRLEIFKDNLKHIDARNRELQITSSYWLGLNEFSDMSHEEFKNKYL 402
            F SWM +H K+Y S E   HRL++F +N + I A N   Q   ++ +GLN+FSDMS  E K+KYL
Sbjct:   33 FTSWMKQHQKTYSSREYS-HRLQVFANNWRKIQAHN---QRNHTFKMGLNQFSDMSFAEIKHKYL 93

HSP 2 Score: 49.6766 bits (117), Expect = 2.393e-17
Identity = 22/45 (48.89%), Postives = 28/45 (62.22%), Query Frame = 3

Query:  486 SFFVQNVEDLPQSVDWRKKG-AVTHVKNQNPCGGCWAFSAVAAVE 617
            S +++     P S+DWRKKG  V+ VKNQ  CG CW FS   A+E
Sbjct:  105 SNYLRGTGPYPSSMDWRKKGNVVSPVKNQGACGSCWTFSTTGALE 149

[Close]

BLAST of CF503965 vs. ExPASy Swiss-Prot
Match: RD19A_ARATH (Cysteine proteinase RD19a OS=Arabidopsis thaliana GN=RD19A PE=2 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 3.103e-17
Identity = 26/44 (59.09%), Postives = 31/44 (70.45%), Query Frame = 3

Query:  507 EDLPQSVDWRKKGAVTHVKNQNPCGGCWAFSAVAAVEGINQIVT 638
            E+LP+  DWR  GAVT VKNQ  CG CW+FSA  A+EG N + T
Sbjct:  133 ENLPEDFDWRDHGAVTPVKNQGSCGSCWSFSATGALEGANFLAT 176

HSP 2 Score: 46.595 bits (109), Expect = 3.103e-17
Identity = 29/81 (35.80%), Postives = 47/81 (58.02%), Query Frame = 1

Query:  160 GYSLEVLTSTDKLVELFESWMLKHGKSYESTEEKLHRLEIFKDNLKHIDARNRELQITSSYWLGLNEFSDMSHEEFKNKYL 402
            G   +VLTS D     F  +  K GK Y S EE  +R  +FK NL+    R+++L  ++++  G+ +FSD++  EF+ K+L
Sbjct:   39 GAEPQVLTSEDH----FSLFKRKFGKVYASNEEHDYRFSVFKANLRRA-RRHQKLDPSATH--GVTQFSDLTRSEFRKKHL 112

[Close]

BLAST of CF503965 vs. ExPASy Swiss-Prot
Match: CYSP6_DICDI (Cysteine proteinase 6 OS=Dictyostelium discoideum GN=cprF PE=2 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 1.147e-16
Identity = 25/37 (67.57%), Postives = 27/37 (72.97%), Query Frame = 3

Query:  522 SVDWRKKGAVTHVKNQNPCGGCWAFSAVAAVEGINQI 632
            SVDWR KGAVT +KNQ  CGGCW+FSA  A EG   I
Sbjct:  115 SVDWRAKGAVTPIKNQGECGGCWSFSATGATEGAQYI 151

HSP 2 Score: 47.3654 bits (111), Expect = 1.147e-16
Identity = 31/83 (37.35%), Postives = 48/83 (57.83%), Query Frame = 1

Query:  169 LEVLTSTDKLVEL-----FESWMLKHGKSYESTEEKLHRLEIFKDNLKHIDARNRELQITSSYWLGLNEFSDMSHEEFKNKYL 402
            + V T+  +L EL     F +WM+ H + Y S+EE   R  IFK N+ +I+  N +    S   LGLN F+D+++EE++  YL
Sbjct:   12 VSVATAKQQLSELQYRNAFTNWMIAHQRHY-SSEEFNGRFNIFKANMDYINEWNTK---GSETVLGLNVFADITNEEYRATYL 90

[Close]

BLAST of CF503965 vs. ExPASy Swiss-Prot
Match: CATL_SARPE (Cathepsin L OS=Sarcophaga peregrina PE=1 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 1.155e-16
Identity = 23/36 (63.89%), Postives = 29/36 (80.56%), Query Frame = 3

Query:  513 LPQSVDWRKKGAVTHVKNQNPCGGCWAFSAVAAVEG 620
            +P+SVDWR+ GAVT VK+Q  CG CWAFS+  A+EG
Sbjct:  122 VPKSVDWREHGAVTGVKDQGHCGSCWAFSSTGALEG 157

HSP 2 Score: 50.0618 bits (118), Expect = 1.155e-16
Identity = 27/75 (36.00%), Postives = 48/75 (64.00%), Query Frame = 1

Query:  172 EVLTSTDKLVELFESWMLKHGKSYESTEEKLHRLEIFKDNLKHIDARNREL--QITSSYWLGLNEFSDMSHEEFK 390
            + ++  D + E + ++ L+H K+Y +  E+  R++IF +N +H  A++ +L  Q   SY LGLN+++DM H EFK
Sbjct:   16 QAISPLDLIKEEWHTYKLQHRKNYANEVEERFRMKIFNEN-RHKIAKHNQLFAQGKVSYKLGLNKYADMLHHEFK 89

[Close]

BLAST of CF503965 vs. ExPASy Swiss-Prot
Match: CYSP2_DICDI (Cysteine proteinase 2 OS=Dictyostelium discoideum GN=cprB PE=2 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 3.287e-16
Identity = 26/51 (50.98%), Postives = 34/51 (66.67%), Query Frame = 3

Query:  495 VQNVEDL---PQSVDWRKKGAVTHVKNQNPCGGCWAFSAVAAVEGINQIVT 638
            V NVEDL   P+S+DWR K AVT +K+Q  CG CW+FS   + EG + + T
Sbjct:  114 VLNVEDLQTNPKSIDWRTKNAVTPIKDQGQCGSCWSFSTTGSTEGAHALKT 164

HSP 2 Score: 46.595 bits (109), Expect = 3.287e-16
Identity = 25/65 (38.46%), Postives = 38/65 (58.46%), Query Frame = 1

Query:  208 FESWMLKHGKSYESTEEKLHRLEIFKDNLKHIDARNRELQITSSYWLGLNEFSDMSHEEFKNKYL 402
            F  W LK  + Y S+E   +R  IFK N+ ++D  N +    S   LGLN F+D+++EE++  YL
Sbjct:   36 FTEWTLKFNRQYSSSEFS-NRYSIFKSNMDYVDNWNSKGD--SQTVLGLNNFADITNEEYRKTYL 97

[Close]

BLAST of CF503965 vs. ExPASy Swiss-Prot
Match: CATK_MACMU (Cathepsin K OS=Macaca mulatta GN=CTSK PE=1 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 7.242e-16
Identity = 25/35 (71.43%), Postives = 28/35 (80.00%), Query Frame = 3

Query:  516 PQSVDWRKKGAVTHVKNQNPCGGCWAFSAVAAVEG 620
            P SVD+RKKG VT VKNQ  CG CWAFS+V A+EG
Sbjct:  116 PDSVDYRKKGYVTPVKNQGQCGSCWAFSSVGALEG 150

HSP 2 Score: 46.2098 bits (108), Expect = 7.242e-16
Identity = 26/70 (37.14%), Postives = 39/70 (55.71%), Query Frame = 1

Query:  208 FESWMLKHGKSYESTEEKLHRLEIFKDNLKHIDARNRELQI-TSSYWLGLNEFSDMSHEEFKNKYLTGLK 414
            +E W   H K Y S  +++ R  I++ NLK+I   N E  +   +Y L +N   DM++EE   K +TGLK
Sbjct:   26 WELWKKTHRKQYNSKVDEISRRLIWEKNLKYISIHNLEASLGVHTYELAMNHLGDMTNEEVVQK-MTGLK 94

[Close]

BLAST of CF503965 vs. ExPASy Swiss-Prot
Match: CATK_MACFA (Cathepsin K OS=Macaca fascicularis GN=CTSK PE=2 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 7.242e-16
Identity = 25/35 (71.43%), Postives = 28/35 (80.00%), Query Frame = 3

Query:  516 PQSVDWRKKGAVTHVKNQNPCGGCWAFSAVAAVEG 620
            P SVD+RKKG VT VKNQ  CG CWAFS+V A+EG
Sbjct:  116 PDSVDYRKKGYVTPVKNQGQCGSCWAFSSVGALEG 150

HSP 2 Score: 46.2098 bits (108), Expect = 7.242e-16
Identity = 26/70 (37.14%), Postives = 39/70 (55.71%), Query Frame = 1

Query:  208 FESWMLKHGKSYESTEEKLHRLEIFKDNLKHIDARNRELQI-TSSYWLGLNEFSDMSHEEFKNKYLTGLK 414
            +E W   H K Y S  +++ R  I++ NLK+I   N E  +   +Y L +N   DM++EE   K +TGLK
Sbjct:   26 WELWKKTHRKQYNSKVDEISRRLIWEKNLKYISIHNLEASLGVHTYELAMNHLGDMTNEEVVQK-MTGLK 94

[Close]

BLAST of CF503965 vs. ExPASy Swiss-Prot
Match: CATK_RABIT (Cathepsin K OS=Oryctolagus cuniculus GN=CTSK PE=1 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 9.413e-16
Identity = 24/35 (68.57%), Postives = 28/35 (80.00%), Query Frame = 3

Query:  516 PQSVDWRKKGAVTHVKNQNPCGGCWAFSAVAAVEG 620
            P S+D+RKKG VT VKNQ  CG CWAFS+V A+EG
Sbjct:  116 PDSIDYRKKGYVTPVKNQGQCGSCWAFSSVGALEG 150

HSP 2 Score: 46.2098 bits (108), Expect = 9.413e-16
Identity = 26/70 (37.14%), Postives = 38/70 (54.29%), Query Frame = 1

Query:  208 FESWMLKHGKSYESTEEKLHRLEIFKDNLKHIDARNRELQI-TSSYWLGLNEFSDMSHEEFKNKYLTGLK 414
            +E W   + K Y S  +++ R  I++ NLKHI   N E  +   +Y L +N   DM+ EE   K +TGLK
Sbjct:   26 WELWKKTYSKQYNSKVDEISRRLIWEKNLKHISIHNLEASLGVHTYELAMNHLGDMTSEEVVQK-MTGLK 94

[Close]

BLAST of CF503965 vs. ExPASy Swiss-Prot
Match: CATK_RAT (Cathepsin K OS=Rattus norvegicus GN=Ctsk PE=2 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 9.413e-16
Identity = 23/36 (63.89%), Postives = 28/36 (77.78%), Query Frame = 3

Query:  513 LPQSVDWRKKGAVTHVKNQNPCGGCWAFSAVAAVEG 620
            +P S+D+RKKG VT VKNQ  CG CWAFS+  A+EG
Sbjct:  115 VPDSIDYRKKGYVTPVKNQGQCGSCWAFSSAGALEG 150

HSP 2 Score: 47.3654 bits (111), Expect = 9.413e-16
Identity = 28/80 (35.00%), Postives = 42/80 (52.50%), Query Frame = 1

Query:  178 LTSTDKLVELFESWMLKHGKSYESTEEKLHRLEIFKDNLKHIDARNRELQI-TSSYWLGLNEFSDMSHEEFKNKYLTGLK 414
            L+  + L   +E W   HGK Y S  +++ R  I++ NLK I   N E  +   +Y L +N   DM+ EE   K +TGL+
Sbjct:   16 LSPEETLDTQWELWKKTHGKQYNSKVDEISRRLIWEKNLKKISVHNLEASLGAHTYELAMNHLGDMTSEEVVQK-MTGLR 94

The following BLAST results are available for this feature:

BLAST of CF503965 vs. ExPASy Swiss-Prot

BLAST of CF503965 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 125


Match Name	E-value	Identity	Description
CATLL_FASHE	1.089e-17	36.17	Cathepsin L-like proteinase OS=Fasciola hepatica G...	[more]
CATH_RAT	2.393e-17	47.69	Cathepsin H OS=Rattus norvegicus GN=Ctsh PE=1 SV=1	[more]
RD19A_ARATH	3.103e-17	59.09	Cysteine proteinase RD19a OS=Arabidopsis thaliana ...	[more]
CYSP6_DICDI	1.147e-16	67.57	Cysteine proteinase 6 OS=Dictyostelium discoideum ...	[more]
CATL_SARPE	1.155e-16	63.89	Cathepsin L OS=Sarcophaga peregrina PE=1 SV=1	[more]
CYSP2_DICDI	3.287e-16	50.98	Cysteine proteinase 2 OS=Dictyostelium discoideum ...	[more]
CATK_MACMU	7.242e-16	71.43	Cathepsin K OS=Macaca mulatta GN=CTSK PE=1 SV=1	[more]
CATK_MACFA	7.242e-16	71.43	Cathepsin K OS=Macaca fascicularis GN=CTSK PE=2 SV...	[more]
CATK_RABIT	9.413e-16	68.57	Cathepsin K OS=Oryctolagus cuniculus GN=CTSK PE=1 ...	[more]
CATK_RAT	9.413e-16	63.89	Cathepsin K OS=Rattus norvegicus GN=Ctsk PE=2 SV=1	[more]

Pages

Properties


Property Name	Value
Genbank description	USDA-FP_119000-453 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-11_G08 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF503965 ID=CF503965; Name=CF503965; organism=Citrus sinensis; type=EST; length=639bp
GTGGGGGAGAGAGAGAGAGAGAGAGAGATGAAAATGTTATCTTCCAATTG
TAAGTTGTTGCTTCGTCTTTCTTTGTGCGTGTCTCTCTTGATGATCATAT
GCATACGCTGCTCTAGCAGTAGAACTCTTCAAGGTCATGATGGTGAGTTC
TCCATTGTGGGGTATTCACTAGAGGTGTTGACATCCACGGACAAGCTGGT
TGAGCTATTCGAGTCATGGATGTTGAAACATGGGAAATCGTATGAAAGCA
CGGAAGAGAAGTTGCATAGATTGGAGATATTCAAAGACAACTTGAAGCAC
ATTGATGCGAGGAACAGAGAGCTGCAAATTACTAGTAGCTATTGGCTTGG
TCTGAATGAGTTTTCAGATATGAGCCACGAAGAGTTCAAGAACAAGTACT
TAACAGGACTCAAACCCGATGATGATGAGTTCCGCAGAAGGAGTCACCGC
GACTCCAGTAGTAGATCTTCTGCTAGCAGTAGTAATCATTTTTTGTACAA
AATGTAGAGGATTTGCCTCAATCCGTTGACTGGAGGAAGAAAGGAGCTGT
CACTCATGTCAAAAACCAAAATCCTTGTGGTGGATGTTGGGCATTTTCAG
CAGTAGCAGCAGTGGAGGGGATAAATCAGATAGTGACGG