CF504033

Overview
NameCF504033
Unique NameCF504033
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length574
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CF504033 vs. ExPASy Swiss-Prot
Match: TBE_MOUSE (Tubulin epsilon chain OS=Mus musculus GN=Tube1 PE=2 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 1.549e-16
Identity = 48/184 (26.09%), Postives = 92/184 (50.00%), Query Frame = 3
Query:   24 LDRIRKLADNCTGLQGFLVFNAVXXXXXXXXXXXXXXXXXVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDI---------------------------ERPTYTNLNRLVSQVISSLTASLRFDGALNVDVTEFQTNLVPYPRIHFMLSSYAPV 494
            L+++RK A+ C  LQ F + +++                  ++ +  +    VYPS +    +  PYNS+L+   L EH D  + +DN++++DI  + +D+                            +  +  +N +V+ ++ SLT+S RF+G+LN+D+ E   NLVP+P++H+++SS  P+
Sbjct:  125 LEKLRKSAEQCDCLQCFFIIHSMGGGTGSGLGTFLLKVLEDEFPEVYRFVTAVYPSSEDDV-ITSPYNSMLAMKELNEHADCVLPIDNQSLFDIISK-IDLVVNSGKLGSAVKPKSLITSNMGAVKKHHKKPFDAMNNIVANLLLSLTSSARFEGSLNMDLNEISMNLVPFPKLHYLVSSLTPL 306          
BLAST of CF504033 vs. ExPASy Swiss-Prot
Match: TBE_HUMAN (Tubulin epsilon chain OS=Homo sapiens GN=TUBE1 PE=2 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 2.023e-16
Identity = 47/187 (25.13%), Postives = 94/187 (50.27%), Query Frame = 3
Query:   15 DLCLDRIRKLADNCTGLQGFLVFNAVXXXXXXXXXXXXXXXXXVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDI---------------------------ERPTYTNLNRLVSQVISSLTASLRFDGALNVDVTEFQTNLVPYPRIHFMLSSYAPV 494
            D  L++ RK A++C  LQ F + +++                  ++ +  +   ++YPS +    +  PYNS+L+   L EH D  + +DN++++DI  + +D+                            +  +  +N +V+ ++ +LT+S RF+G+LN+D+ E   NLVP+P++H+++SS  P+
Sbjct:  122 DQILEKFRKSAEHCDCLQCFFIIHSMGGGTGSGLGTFLLKVLEDEFPEVYRFVTSIYPSGEDDV-ITSPYNSILAMKELNEHADCVLPIDNQSLFDIISK-IDLMVNSGKLGTTVKPKSLVTSSSGALKKQHKKPFDAMNNIVANLLLNLTSSARFEGSLNMDLNEISMNLVPFPQLHYLVSSLTPL 306          
BLAST of CF504033 vs. ExPASy Swiss-Prot
Match: TBB3_ANEPH (Tubulin beta-3 chain (Fragment) OS=Anemia phyllitidis GN=TUBB3 PE=2 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 2.642e-16
Identity = 38/95 (40.00%), Postives = 60/95 (63.16%), Query Frame = 3
Query:  288 EAIYDICRRSLDIERPTYTNLNRLVSQVISSLTASLRFDGALNVDVTEFQTNLVPYPRIHFMLSSYAPVISAEKAFHEQLSVAEITNSAFEPSSM 572
            EA+YDIC R+L +  PT+ +LN L+S  +S +T  LRF G LN D+ +   NL+P+PR+HF +  +AP+ S     +  L+V E+T   ++  +M
Sbjct:    1 EALYDICFRTLKLVTPTFGDLNHLISATMSGVTCCLRFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTSRGSQQYRALTVPELTQQMWDAKNM 95          
BLAST of CF504033 vs. ExPASy Swiss-Prot
Match: TBG_ASHGO (Tubulin gamma chain OS=Ashbya gossypii GN=TUB4 PE=3 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 5.887e-16
Identity = 49/162 (30.25%), Postives = 84/162 (51.85%), Query Frame = 3
Query:   15 DLCLDRIRKLADNCTGLQGFLVFNAVXXXXXXXXXXXXXXXXXVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPTYTNLNRLVSQVISSLTASLRFDGALNVDVTEFQTNLVPYPRIHFMLSSYAPVIS 500
            D  ++ I K  D     +GF + ++V                   Y KK    ++V+PS + S  VV+PYN++L+   L++++D +VL DN+A+ ++  R L     +Y   N+L++ V+SS+T SLRF   +   +    + LVP P +HF+  S+ P  S
Sbjct:  118 DEFMNMIDKEIDATGNFEGFQLIHSVAGGTGSGLGSNLLEALSDRYHKKIVSTYSVFPSRE-SEVVVQPYNTILTLRRLIDNSDASVLFDNDALLNLTARVLRDSNTSYQQTNQLIASVMSSVTNSLRFPSYMYNSLPSIFSTLVPTPELHFLAPSFTPFTS 278          
BLAST of CF504033 vs. ExPASy Swiss-Prot
Match: TBG_CANGA (Tubulin gamma chain OS=Candida glabrata GN=TUB4 PE=3 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 6.508e-15
Identity = 45/159 (28.30%), Postives = 78/159 (49.06%), Query Frame = 3
Query:   15 DLCLDRIRKLADNCTGLQGFLVFNAVXXXXXXXXXXXXXXXXXVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPTYTNLNRLVSQVISSLTASLRFDGALNVDVTEFQTNLVPYPRIHFMLSSYAP 491
            D  +D I +  D+    +GF + ++V                   Y K     ++V+PS Q S  VV PYN+VL+   L E  D +++ DN A+ ++  R+      +Y + N+L+S  +SS+T SLRF   +   +    + L+P P +HF+  ++ P
Sbjct:  118 DRIMDIIDRELDSTDNFEGFQLLHSVAGGTGSGLGSSLLEALTDRYSKSFITTYSVFPSKQ-SEVVVGPYNTVLTLRRLCEDADASIIFDNNALLNLTARTFRDPNTSYEHTNQLISSALSSITNSLRFPSYMYNSMASIFSTLIPTPELHFLTPNFTP 275          
BLAST of CF504033 vs. ExPASy Swiss-Prot
Match: TBG_YEAST (Tubulin gamma chain OS=Saccharomyces cerevisiae GN=TUB4 PE=1 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 3.571e-13
Identity = 44/162 (27.16%), Postives = 81/162 (50.00%), Query Frame = 3
Query:   15 DLCLDRIRKLADNCTGLQGFLVFNAVXXXXXXXXXXXXXXXXXVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPTYTNLNRLVSQVISSLTASLRFDGALNVDVTEFQTNLVPYPRIHFMLSSYAPVIS 500
            D  L++I K  D+    +GF + ++V                   Y KK    ++V+P+ + S  VV+ YN++L+   L+E +D  V+ DN ++ +I  +          + N+L+S +ISS+T S+RF   +   ++   + L+P P +HF+  S+ P  S
Sbjct:  117 DDILNKIDKEIDSTDNFEGFQLLHSVAGGTGSGLGSNLLEALCDRYPKKILTTYSVFPA-RSSEVVVQSYNTILALRRLIEDSDATVVFDNASLLNISGKVFRNPNIDLQHTNQLISTIISSVTNSIRFPSYMYSSMSSIYSTLIPSPELHFLSPSFTPFTS 277          
BLAST of CF504033 vs. ExPASy Swiss-Prot
Match: TBG_ENTHI (Tubulin gamma chain OS=Entamoeba histolytica PE=2 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 8.796e-12
Identity = 44/182 (24.18%), Postives = 80/182 (43.96%), Query Frame = 3
Query:   33 IRKLADNCTGLQGFLVFNAVXXXXXXXXXXXXXXXXXVDYGKKSKLGFTVY--PSPQVSTSVVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPTYTNLNRLVSQVISSLTASLRFDGALNVDVTEFQTNLVPYPRIHFMLSSYAPVISAEKAFHEQLSVAEITNSAFEPSSM 572
            I +  ++C  L+GF   +++                   Y K     F+V    +P V   VV PYNS+L+   L+      V+ DN A+ DI      ++  T  ++N ++S  +S+ TA+LRF   L   +     +L P    HF+++SY P+    +    + S  ++     +P ++
Sbjct:  119 IDREVEHCDSLEGFFFCHSICGGTGSGLGSKIMEMISEKYPKNILTSFSVMVKENPDV---VVSPYNSILTLRRLITECQSVVVFDNSALADITHTQFGVDEATVFDMNSIISSSMSAFTANLRFPSTLYNSLNSLMIHLCPSRFSHFLMTSYTPLRQVNQ-ISSRTSAIDVMKRLIQPQNI 296          
BLAST of CF504033 vs. ExPASy Swiss-Prot
Match: TBA_POPEU (Tubulin alpha chain (Fragments) OS=Populus euphratica PE=1 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 5.702e-11
Identity = 35/50 (70.00%), Postives = 36/50 (72.00%), Query Frame = 3
Query:  312 RSLDIERPTYTNLNRLVSQVISSLTASLRFDGALNVDVTEFQTNLVPYPR 461
            RSLDIERPTYTNLNR              FDGA+NVDVTEFQTNLVPYPR
Sbjct:   15 RSLDIERPTYTNLNR--------------FDGAINVDVTEFQTNLVPYPR 50          
BLAST of CF504033 vs. ExPASy Swiss-Prot
Match: TBG_CANAL (Tubulin gamma chain OS=Candida albicans GN=TUB4 PE=3 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 9.726e-11
Identity = 47/189 (24.87%), Postives = 93/189 (49.21%), Query Frame = 3
Query:   24 LDRIRKLADNCTGLQGFLVFNAVXXXXXXXXXXXXXXXXXVDYGKKSKLG-FTVYPSPQ-VSTSVVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDI----CRRSLDIERPT---YTNLNRLVSQVISSLTASLRFDGALNVDVTEFQTNLVPYPRIHFMLSSYAPVISAEKAF-HEQLSVAEITNSAFE 560
            L+ I +  D C  L  F +F++V                   YG K  L  F+++PS +  S  VV+PYN++L+   L++++D   +  N+++  I       + +I+      +   N+L++ V +S++  LRF G +   +    +NL+P P + F+ SS AP  + +  + +E   + E++N  ++
Sbjct:  146 LNLIDREVDKCDNLSNFQLFHSVAGGTGSGVGSKMLEVISDRYGHKKLLNTFSIFPSNEDTSDVVVQPYNTILTLKRLIDYSDATFVFHNDSLNRIENILFNNNSNIQHDDNDLFLGANKLIALVSASVSNPLRFPGYMYSSMESIVSNLIPTPDLKFLTSSIAPFSTQKHNYLNEYDMLLELSNDRYK 334          
The following BLAST results are available for this feature:
BLAST of CF504033 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 419
Match NameE-valueIdentityDescription
TBE_MOUSE1.549e-1626.09Tubulin epsilon chain OS=Mus musculus GN=Tube1 PE=... [more]
TBE_HUMAN2.023e-1625.13Tubulin epsilon chain OS=Homo sapiens GN=TUBE1 PE=... [more]
TBB3_ANEPH2.642e-1640.00Tubulin beta-3 chain (Fragment) OS=Anemia phylliti... [more]
TBG_ASHGO5.887e-1630.25Tubulin gamma chain OS=Ashbya gossypii GN=TUB4 PE=... [more]
TBG_CANGA6.508e-1528.30Tubulin gamma chain OS=Candida glabrata GN=TUB4 PE... [more]
TBG_YEAST3.571e-1327.16Tubulin gamma chain OS=Saccharomyces cerevisiae GN... [more]
TBG_ENTHI8.796e-1224.18Tubulin gamma chain OS=Entamoeba histolytica PE=2 ... [more]
TBA_POPEU5.702e-1170.00Tubulin alpha chain (Fragments) OS=Populus euphrat... [more]
TBG_CANAL9.726e-1124.87Tubulin gamma chain OS=Candida albicans GN=TUB4 PE... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_119000-521 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-12_E11 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF504033 ID=CF504033; Name=CF504033; organism=Citrus sinensis; type=EST; length=574bp
GGAAGGAGATTGTTGATCTATGCTTGGACCGGATCAGAAAACTTGCTGAT
AATTGCACTGGGCTACAAGGGTTCCTGGTTTTCAATGCTGTTGGAGGTGG
TACTGGCTCTGGACTTGGGTCACTTCTCTTGGAGCGTCTCTCTGTTGACT
ATGGAAAGAAATCAAAGCTTGGTTTCACTGTGTATCCTTCACCTCAGGTT
TCCACATCAGTTGTGGAACCTTACAACAGTGTTCTTTCAACACATTCCCT
CCTTGAGCACACCGATGTTGCTGTGCTTCTTGATAATGAAGCCATCTATG
ATATCTGCAGGCGCTCTCTTGACATTGAGCGCCCCACTTACACCAATCTC
AACCGACTTGTCTCTCAGGTGATCTCATCCCTCACTGCCTCTTTGAGGTT
TGATGGAGCTCTGAATGTGGATGTGACTGAGTTCCAGACCAATTTGGTTC
CCTATCCAAGGATCCATTTCATGCTCTCCTCTTATGCCCCAGTTATCTCT
GCAGAGAAGGCATTCCATGAGCAACTTTCAGTTGCTGAGATCACCAACAG
TGCTTTTGAGCCATCATCCATGAT
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