CF504111 | Citrus Genome Database

Overview


Name	CF504111
Unique Name	CF504111
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	256

Libraries


Library Name	Type
Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CF504111 vs. ExPASy Swiss-Prot
Match: TBB8_HUMAN (Tubulin beta-8 chain OS=Homo sapiens GN=TUBB8 PE=1 SV=2)

HSP 1 Score: 62.3882 bits (150), Expect = 3.150e-19
Identity = 27/39 (69.23%), Postives = 30/39 (76.92%), Query Frame = 3

Query:   27 MREILHIQGGQCGNQIGSKFWEVICDEHGVDPTGKYRGD 143
            MREI+  Q GQCGNQIG+KFWEVI DEH +D  G Y GD
Sbjct:    1 MREIVLTQIGQCGNQIGAKFWEVISDEHAIDSAGTYHGD 39

HSP 2 Score: 44.669 bits (104), Expect = 3.150e-19
Identity = 20/21 (95.24%), Postives = 21/21 (100.00%), Query Frame = 2

Query:  191 EASGGRYVPRAVLMDLEPGTM 253
            EASGGRYVPRAVL+DLEPGTM
Sbjct:   53 EASGGRYVPRAVLVDLEPGTM 73

HSP 3 Score: 27.7202 bits (60), Expect = 3.150e-19
Identity = 12/17 (70.59%), Postives = 13/17 (76.47%), Query Frame = 1

Query:  145 GVEDLQLERINVYYNRS 195
            G   LQLERINVYYN +
Sbjct:   38 GDSHLQLERINVYYNEA 54

[Close]

BLAST of CF504111 vs. ExPASy Swiss-Prot
Match: TBB_ENTDO (Tubulin beta chain OS=Enteroctopus dofleini PE=2 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 3.684e-19
Identity = 30/38 (78.95%), Postives = 35/38 (92.11%), Query Frame = 3

Query:   27 MREILHIQGGQCGNQIGSKFWEVICDEHGVDPTGKYRG 140
            MREI+HIQ GQCGNQIGSKFWEVI +EHG+DP+G Y+G
Sbjct:    1 MREIVHIQAGQCGNQIGSKFWEVISEEHGIDPSGVYQG 38

HSP 2 Score: 41.5874 bits (96), Expect = 3.684e-19
Identity = 18/21 (85.71%), Postives = 20/21 (95.24%), Query Frame = 2

Query:  191 EASGGRYVPRAVLMDLEPGTM 253
            EAS G+YVPRAVL+DLEPGTM
Sbjct:   53 EASSGKYVPRAVLLDLEPGTM 73

[Close]

BLAST of CF504111 vs. ExPASy Swiss-Prot
Match: TBB2_PHYPO (Tubulin beta-2 chain OS=Physarum polycephalum GN=BETC PE=2 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 4.077e-19
Identity = 26/39 (66.67%), Postives = 34/39 (87.18%), Query Frame = 3

Query:   27 MREILHIQGGQCGNQIGSKFWEVICDEHGVDPTGKYRGD 143
            MREI+H+Q GQCGNQ+G+KFWEV+ +EHG+D  G Y+GD
Sbjct:    1 MREIVHVQVGQCGNQVGAKFWEVVSEEHGIDSAGTYKGD 39

HSP 2 Score: 35.4242 bits (80), Expect = 4.077e-19
Identity = 14/21 (66.67%), Postives = 18/21 (85.71%), Query Frame = 2

Query:  191 EASGGRYVPRAVLMDLEPGTM 253
            E +G +YVPRAVL+DLEPG +
Sbjct:   53 EVAGSKYVPRAVLVDLEPGVL 73

HSP 3 Score: 29.6462 bits (65), Expect = 4.077e-19
Identity = 13/15 (86.67%), Postives = 13/15 (86.67%), Query Frame = 1

Query:  145 GVEDLQLERINVYYN 189
            G  DLQLERINVYYN
Sbjct:   38 GDTDLQLERINVYYN 52

[Close]

BLAST of CF504111 vs. ExPASy Swiss-Prot
Match: TBB_MELLI (Tubulin beta chain OS=Melampsora lini GN=TUB1 PE=3 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 5.285e-19
Identity = 26/38 (68.42%), Postives = 33/38 (86.84%), Query Frame = 3

Query:   27 MREILHIQGGQCGNQIGSKFWEVICDEHGVDPTGKYRG 140
            MREI+H+Q GQCGNQIG+KFWEV+ DEHG+   G+Y+G
Sbjct:    1 MREIVHLQTGQCGNQIGAKFWEVVSDEHGIATDGQYKG 38

HSP 2 Score: 38.5058 bits (88), Expect = 5.285e-19
Identity = 16/21 (76.19%), Postives = 19/21 (90.48%), Query Frame = 2

Query:  191 EASGGRYVPRAVLMDLEPGTM 253
            E +G +YVPRAVL+DLEPGTM
Sbjct:   53 EVAGNKYVPRAVLIDLEPGTM 73

HSP 3 Score: 29.261 bits (64), Expect = 5.285e-19
Identity = 12/15 (80.00%), Postives = 13/15 (86.67%), Query Frame = 1

Query:  145 GVEDLQLERINVYYN 189
            G  DLQLERI+VYYN
Sbjct:   38 GTSDLQLERISVYYN 52

[Close]

BLAST of CF504111 vs. ExPASy Swiss-Prot
Match: TBB_PORYE (Tubulin beta chain OS=Porphyra yezoensis GN=TUBB1 PE=3 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 6.841e-19
Identity = 28/38 (73.68%), Postives = 33/38 (86.84%), Query Frame = 3

Query:   27 MREILHIQGGQCGNQIGSKFWEVICDEHGVDPTGKYRG 140
            MREI+HIQ GQCGNQIG+KFWEVI +EHG+D +G Y G
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISEEHGIDSSGAYIG 38

HSP 2 Score: 40.0466 bits (92), Expect = 6.841e-19
Identity = 17/21 (80.95%), Postives = 19/21 (90.48%), Query Frame = 2

Query:  191 EASGGRYVPRAVLMDLEPGTM 253
            E SGGRYVPRAVL+DLEPG +
Sbjct:   53 EGSGGRYVPRAVLVDLEPGVL 73

HSP 3 Score: 25.7942 bits (55), Expect = 6.841e-19
Identity = 10/15 (66.67%), Postives = 11/15 (73.33%), Query Frame = 1

Query:  145 GVEDLQLERINVYYN 189
            G  DLQL+R  VYYN
Sbjct:   38 GTSDLQLDRAEVYYN 52

[Close]

BLAST of CF504111 vs. ExPASy Swiss-Prot
Match: TBB_USTMA (Tubulin beta chain OS=Ustilago maydis GN=UM05828 PE=3 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 6.847e-19
Identity = 26/38 (68.42%), Postives = 32/38 (84.21%), Query Frame = 3

Query:   27 MREILHIQGGQCGNQIGSKFWEVICDEHGVDPTGKYRG 140
            MREI+H+Q GQCGNQ+G+KFWEV+ DEHG+D  G Y G
Sbjct:    1 MREIVHLQTGQCGNQVGTKFWEVLSDEHGIDHNGNYIG 38

HSP 2 Score: 41.2022 bits (95), Expect = 6.847e-19
Identity = 18/21 (85.71%), Postives = 20/21 (95.24%), Query Frame = 2

Query:  191 EASGGRYVPRAVLMDLEPGTM 253
            EASG +YVPRAVL+DLEPGTM
Sbjct:   53 EASGNKYVPRAVLVDLEPGTM 73

HSP 3 Score: 26.1794 bits (56), Expect = 6.847e-19
Identity = 11/17 (64.71%), Postives = 12/17 (70.59%), Query Frame = 1

Query:  145 GVEDLQLERINVYYNRS 195
            G  D QL RINVYYN +
Sbjct:   38 GTSDDQLARINVYYNEA 54

[Close]

BLAST of CF504111 vs. ExPASy Swiss-Prot
Match: TBB_ENCHE (Tubulin beta chain OS=Encephalitozoon hellem GN=TUB2 PE=3 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 8.877e-19
Identity = 27/38 (71.05%), Postives = 31/38 (81.58%), Query Frame = 3

Query:   27 MREILHIQGGQCGNQIGSKFWEVICDEHGVDPTGKYRG 140
            MREI+H+Q GQCGNQ+G KFWE I  EHG+D TGKY G
Sbjct:    1 MREIIHLQTGQCGNQVGCKFWETISGEHGIDQTGKYVG 38

HSP 2 Score: 38.1206 bits (87), Expect = 8.877e-19
Identity = 17/21 (80.95%), Postives = 19/21 (90.48%), Query Frame = 2

Query:  191 EASGGRYVPRAVLMDLEPGTM 253
            EAS  +YVPRAVL+DLEPGTM
Sbjct:   53 EASSKKYVPRAVLIDLEPGTM 73

HSP 3 Score: 28.4906 bits (62), Expect = 8.877e-19
Identity = 11/17 (64.71%), Postives = 13/17 (76.47%), Query Frame = 1

Query:  145 GVEDLQLERINVYYNRS 195
            G  D QLER+NVYYN +
Sbjct:   38 GTSDNQLERVNVYYNEA 54

[Close]

BLAST of CF504111 vs. ExPASy Swiss-Prot
Match: TBB6_MOUSE (Tubulin beta-6 chain OS=Mus musculus GN=Tubb6 PE=1 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 1.149e-18
Identity = 30/39 (76.92%), Postives = 33/39 (84.62%), Query Frame = 3

Query:   27 MREILHIQGGQCGNQIGSKFWEVICDEHGVDPTGKYRGD 143
            MREI+HIQ GQCGNQIG+KFWEVI DEHG+D  G Y GD
Sbjct:    1 MREIVHIQAGQCGNQIGTKFWEVISDEHGIDQAGGYVGD 39

HSP 2 Score: 35.4242 bits (80), Expect = 1.149e-18
Identity = 15/21 (71.43%), Postives = 18/21 (85.71%), Query Frame = 2

Query:  191 EASGGRYVPRAVLMDLEPGTM 253
            E+S  +YVPRA L+DLEPGTM
Sbjct:   53 ESSSKKYVPRAALVDLEPGTM 73

HSP 3 Score: 26.5646 bits (57), Expect = 1.149e-18
Identity = 12/17 (70.59%), Postives = 13/17 (76.47%), Query Frame = 1

Query:  145 GVEDLQLERINVYYNRS 195
            G   LQLERI+VYYN S
Sbjct:   38 GDSALQLERISVYYNES 54

[Close]

BLAST of CF504111 vs. ExPASy Swiss-Prot
Match: TBB8_PANTR (Tubulin beta-8 chain OS=Pan troglodytes GN=TUBB8 PE=3 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 1.150e-18
Identity = 26/39 (66.67%), Postives = 30/39 (76.92%), Query Frame = 3

Query:   27 MREILHIQGGQCGNQIGSKFWEVICDEHGVDPTGKYRGD 143
            MREI+  Q GQCGNQIG+KFWEVI DEH ++  G Y GD
Sbjct:    1 MREIVLTQIGQCGNQIGAKFWEVISDEHAINSAGTYHGD 39

HSP 2 Score: 44.669 bits (104), Expect = 1.150e-18
Identity = 20/21 (95.24%), Postives = 21/21 (100.00%), Query Frame = 2

Query:  191 EASGGRYVPRAVLMDLEPGTM 253
            EASGGRYVPRAVL+DLEPGTM
Sbjct:   53 EASGGRYVPRAVLVDLEPGTM 73

HSP 3 Score: 27.7202 bits (60), Expect = 1.150e-18
Identity = 12/17 (70.59%), Postives = 13/17 (76.47%), Query Frame = 1

Query:  145 GVEDLQLERINVYYNRS 195
            G   LQLERINVYYN +
Sbjct:   38 GDSHLQLERINVYYNEA 54

[Close]

BLAST of CF504111 vs. ExPASy Swiss-Prot
Match: TBB_PNECA (Tubulin beta chain OS=Pneumocystis carinii GN=TUB-B PE=2 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 1.489e-18
Identity = 25/38 (65.79%), Postives = 30/38 (78.95%), Query Frame = 3

Query:   27 MREILHIQGGQCGNQIGSKFWEVICDEHGVDPTGKYRG 140
            MREI+H+Q GQCGNQIG+ FW  I  EHG+D TG Y+G
Sbjct:    1 MREIVHLQTGQCGNQIGASFWSTISGEHGLDSTGVYQG 38

HSP 2 Score: 41.9726 bits (97), Expect = 1.489e-18
Identity = 17/21 (80.95%), Postives = 21/21 (100.00%), Query Frame = 2

Query:  191 EASGGRYVPRAVLMDLEPGTM 253
            EASGG+YVPR++L+DLEPGTM
Sbjct:   53 EASGGKYVPRSILIDLEPGTM 73

HSP 3 Score: 28.8758 bits (63), Expect = 1.489e-18
Identity = 11/17 (64.71%), Postives = 14/17 (82.35%), Query Frame = 1

Query:  145 GVEDLQLERINVYYNRS 195
            G  DLQLER+NVY+N +
Sbjct:   38 GTSDLQLERMNVYFNEA 54

The following BLAST results are available for this feature:

BLAST of CF504111 vs. ExPASy Swiss-Prot

BLAST of CF504111 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 219


Match Name	E-value	Identity	Description
TBB8_HUMAN	3.150e-19	69.23	Tubulin beta-8 chain OS=Homo sapiens GN=TUBB8 PE=1...	[more]
TBB_ENTDO	3.684e-19	78.95	Tubulin beta chain OS=Enteroctopus dofleini PE=2 S...	[more]
TBB2_PHYPO	4.077e-19	66.67	Tubulin beta-2 chain OS=Physarum polycephalum GN=B...	[more]
TBB_MELLI	5.285e-19	68.42	Tubulin beta chain OS=Melampsora lini GN=TUB1 PE=3...	[more]
TBB_PORYE	6.841e-19	73.68	Tubulin beta chain OS=Porphyra yezoensis GN=TUBB1 ...	[more]
TBB_USTMA	6.847e-19	68.42	Tubulin beta chain OS=Ustilago maydis GN=UM05828 P...	[more]
TBB_ENCHE	8.877e-19	71.05	Tubulin beta chain OS=Encephalitozoon hellem GN=TU...	[more]
TBB6_MOUSE	1.149e-18	76.92	Tubulin beta-6 chain OS=Mus musculus GN=Tubb6 PE=1...	[more]
TBB8_PANTR	1.150e-18	66.67	Tubulin beta-8 chain OS=Pan troglodytes GN=TUBB8 P...	[more]
TBB_PNECA	1.489e-18	65.79	Tubulin beta chain OS=Pneumocystis carinii GN=TUB-...	[more]

Pages

Properties


Property Name	Value
Genbank description	USDA-FP_119000-599 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-14_A02 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF504111 ID=CF504111; Name=CF504111; organism=Citrus sinensis; type=EST; length=256bp
CTTCGTTTCCTTTCCGTTTGAGATAAATGAGAGAGATCTTGCACATTCAA
GGAGGCCAATGCGGCAACCAAATCGGATCCAAATTCTGGGAAGTCATTTG
CGACGAGCATGGCGTGGACCCCACGGGGAAGTATAGAGGTGACCGGGGTT
GAGGATCTTCAACTGGAGCGGATCAATGTCTACTACAACCGAAGCTTCCG
GTGGAAGGTACGTGCCTCGGGCTGTTCTCATGGATCTTGAACCCGGTACC
ATGGGA