CF504111 | Citrus Genome Database

Overview


Name	CF504111
Unique Name	CF504111
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	256

Libraries


Library Name	Type
Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig11134	contig	Csv1_Contig11134:898..1151.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CF504111 vs. ExPASy Swiss-Prot
Match: TBB_AJECA (Tubulin beta chain OS=Ajellomyces capsulata GN=TUB2 PE=3 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 9.519e-16
Identity = 25/38 (65.79%), Postives = 29/38 (76.32%), Query Frame = 3

Query:   27 MREILHIQGGQCGNQIGSKFWEVICDEHGVDPTGKYRG 140
            MREI+H+Q GQCGNQIG+ FW+ I  EHGVD  G Y G
Sbjct:    1 MREIVHLQTGQCGNQIGAAFWQTISGEHGVDGAGYYNG 38

HSP 2 Score: 36.1946 bits (82), Expect = 9.519e-16
Identity = 16/21 (76.19%), Postives = 19/21 (90.48%), Query Frame = 2

Query:  191 EASGGRYVPRAVLMDLEPGTM 253
            EA+  +YVPRAVL+DLEPGTM
Sbjct:   53 EAAEKKYVPRAVLVDLEPGTM 73

HSP 3 Score: 26.1794 bits (56), Expect = 9.519e-16
Identity = 10/17 (58.82%), Postives = 14/17 (82.35%), Query Frame = 1

Query:  145 GVEDLQLERINVYYNRS 195
            G  D+QLER+NVY+N +
Sbjct:   38 GSLDIQLERMNVYFNEA 54

[Close]

BLAST of CF504111 vs. ExPASy Swiss-Prot
Match: TBB_CANAL (Tubulin beta chain OS=Candida albicans GN=TUB2 PE=3 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 4.653e-15
Identity = 25/39 (64.10%), Postives = 30/39 (76.92%), Query Frame = 3

Query:   27 MREILHIQGGQCGNQIGSKFWEVICDEHGVDPTGKYRGD 143
            MREI+H+  GQCGNQIG+ FWE IC EHG+D  G Y G+
Sbjct:    1 MREIIHLSTGQCGNQIGAAFWETICGEHGLDNNGTYVGN 39

HSP 2 Score: 38.5058 bits (88), Expect = 4.653e-15
Identity = 16/21 (76.19%), Postives = 20/21 (95.24%), Query Frame = 2

Query:  191 EASGGRYVPRAVLMDLEPGTM 253
            EA+ G+YVPRAVL+DLEPGT+
Sbjct:   53 EATSGKYVPRAVLVDLEPGTI 73

[Close]

BLAST of CF504111 vs. ExPASy Swiss-Prot
Match: TBB1_TRIVI (Tubulin beta-1 chain OS=Trichoderma viride GN=tub1 PE=3 SV=1)

HSP 1 Score: 55.0694 bits (131), Expect = 7.484e-15
Identity = 24/40 (60.00%), Postives = 30/40 (75.00%), Query Frame = 3

Query:   27 MREIL--HIQGGQCGNQIGSKFWEVICDEHGVDPTGKYRG 140
            MREI+  H+Q GQCGNQ+GS FW+ I  EHG+D +G Y G
Sbjct:    1 MREIVSVHLQTGQCGNQVGSAFWQTISGEHGLDSSGVYGG 40

HSP 2 Score: 38.891 bits (89), Expect = 7.484e-15
Identity = 17/21 (80.95%), Postives = 19/21 (90.48%), Query Frame = 2

Query:  191 EASGGRYVPRAVLMDLEPGTM 253
            EAS  +YVPRAVL+DLEPGTM
Sbjct:   55 EASNNKYVPRAVLVDLEPGTM 75

HSP 3 Score: 25.7942 bits (55), Expect = 7.484e-15
Identity = 9/17 (52.94%), Postives = 13/17 (76.47%), Query Frame = 1

Query:  145 GVEDLQLERINVYYNRS 195
            G  D QL+R+NVY+N +
Sbjct:   40 GTSDQQLDRLNVYFNEA 56

[Close]

BLAST of CF504111 vs. ExPASy Swiss-Prot
Match: TBB_YEAST (Tubulin beta chain OS=Saccharomyces cerevisiae GN=TUB2 PE=1 SV=2)

HSP 1 Score: 63.1586 bits (152), Expect = 8.282e-14
Identity = 26/38 (68.42%), Postives = 29/38 (76.32%), Query Frame = 3

Query:   27 MREILHIQGGQCGNQIGSKFWEVICDEHGVDPTGKYRG 140
            MREI+HI  GQCGNQIG+ FWE IC EHG+D  G Y G
Sbjct:    1 MREIIHISTGQCGNQIGAAFWETICGEHGLDFNGTYHG 38

HSP 2 Score: 33.4982 bits (75), Expect = 8.282e-14
Identity = 13/21 (61.90%), Postives = 19/21 (90.48%), Query Frame = 2

Query:  191 EASGGRYVPRAVLMDLEPGTM 253
            EAS G++VPR++ +DLEPGT+
Sbjct:   53 EASSGKWVPRSINVDLEPGTI 73

[Close]

BLAST of CF504111 vs. ExPASy Swiss-Prot
Match: TBB_ASPFU (Tubulin beta chain OS=Aspergillus fumigatus GN=AFUA_7G00250 PE=3 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 1.269e-13
Identity = 21/34 (61.76%), Postives = 28/34 (82.35%), Query Frame = 3

Query:   27 MREILHIQGGQCGNQIGSKFWEVICDEHGVDPTG 128
            MREI+H+Q GQCGNQ+G+ FW+ I  EHG+D +G
Sbjct:    1 MREIVHLQTGQCGNQVGAAFWQTISSEHGLDESG 34

HSP 2 Score: 35.8094 bits (81), Expect = 1.269e-13
Identity = 15/21 (71.43%), Postives = 17/21 (80.95%), Query Frame = 2

Query:  191 EASGGRYVPRAVLMDLEPGTM 253
            E    +YVPRAVL+DLEPGTM
Sbjct:   53 EVGNSKYVPRAVLVDLEPGTM 73

HSP 3 Score: 23.8682 bits (50), Expect = 1.269e-13
Identity = 9/12 (75.00%), Postives = 11/12 (91.67%), Query Frame = 1

Query:  154 DLQLERINVYYN 189
            D QLER+NVY+N
Sbjct:   41 DQQLERMNVYFN 52

[Close]

BLAST of CF504111 vs. ExPASy Swiss-Prot
Match: TBB1_COLGR (Tubulin beta-1 chain OS=Colletotrichum graminicola GN=TUB1 PE=3 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 3.065e-13
Identity = 23/38 (60.53%), Postives = 30/38 (78.95%), Query Frame = 3

Query:   27 MREILHIQGGQCGNQIGSKFWEVICDEHGVDPTGKYRG 140
            MREI+H+Q GQCGNQ+G+ FW+ I  EHG+D  G +RG
Sbjct:    1 MREIIHLQTGQCGNQVGTAFWQTIHGEHGLDQDGVFRG 38

HSP 2 Score: 35.8094 bits (81), Expect = 3.065e-13
Identity = 16/22 (72.73%), Postives = 19/22 (86.36%), Query Frame = 2

Query:  188 TEASGGRYVPRAVLMDLEPGTM 253
            TEA+  +YVPRAVL+DLEP TM
Sbjct:   52 TEAAKQKYVPRAVLVDLEPATM 73

[Close]

BLAST of CF504111 vs. ExPASy Swiss-Prot
Match: TBB1_CHOCR (Tubulin beta-1 chain OS=Chondrus crispus GN=TUBB PE=2 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 1.909e-12
Identity = 25/39 (64.10%), Postives = 29/39 (74.36%), Query Frame = 3

Query:   30 REILHIQGGQCGNQIGSKFWEVICDEHGVDPTGKYRGDR 146
            R I+ +Q GQCGNQ+G KFWE I  EHG+D  GKY GDR
Sbjct:    3 RSIVSLQVGQCGNQVGLKFWEGISAEHGIDVDGKYIGDR 41

HSP 2 Score: 33.8834 bits (76), Expect = 1.909e-12
Identity = 13/21 (61.90%), Postives = 17/21 (80.95%), Query Frame = 2

Query:  191 EASGGRYVPRAVLMDLEPGTM 253
            E+S G YVPRA ++DLEPG +
Sbjct:   55 ESSSGSYVPRAAMLDLEPGVL 75

[Close]

BLAST of CF504111 vs. ExPASy Swiss-Prot
Match: TBB1_COLGL (Tubulin beta-1 chain OS=Colletotrichum gloeosporioides GN=TUB1 PE=3 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 5.427e-12
Identity = 23/42 (54.76%), Postives = 31/42 (73.81%), Query Frame = 3

Query:   27 MREILHIQGGQC---GNQIGSKFWEVICDEHGVDPTGKYRGD 143
            MREI+H+Q GQC   GNQ+G+ FW+ I  EHG+D  G +RG+
Sbjct:    1 MREIIHLQTGQCLIQGNQVGTAFWQTIHHEHGLDHDGYFRGE 42

HSP 2 Score: 36.5798 bits (83), Expect = 5.427e-12
Identity = 16/21 (76.19%), Postives = 18/21 (85.71%), Query Frame = 2

Query:  191 EASGGRYVPRAVLMDLEPGTM 253
            EAS  +YVPRAVL+DLEP TM
Sbjct:   56 EASNNKYVPRAVLVDLEPATM 76

[Close]

BLAST of CF504111 vs. ExPASy Swiss-Prot
Match: TBB2_OOMCK (Tubulin beta-2 chain (Fragment) OS=Oomycete-like sp. (strain MacKay2000) GN=TUBB2 PE=2 SV=1)

HSP 1 Score: 44.669 bits (104), Expect = 3.593e-11
Identity = 19/21 (90.48%), Postives = 21/21 (100.00%), Query Frame = 2

Query:  191 EASGGRYVPRAVLMDLEPGTM 253
            EA+GGRYVPRA+LMDLEPGTM
Sbjct:   28 EATGGRYVPRAILMDLEPGTM 48

HSP 2 Score: 31.9574 bits (71), Expect = 3.593e-11
Identity = 12/14 (85.71%), Postives = 12/14 (85.71%), Query Frame = 3

Query:  102 DEHGVDPTGKYRGD 143
            DEHGVDPTG Y GD
Sbjct:    1 DEHGVDPTGTYHGD 14

HSP 3 Score: 30.4166 bits (67), Expect = 3.593e-11
Identity = 13/17 (76.47%), Postives = 14/17 (82.35%), Query Frame = 1

Query:  145 GVEDLQLERINVYYNRS 195
            G  DLQLERINVYYN +
Sbjct:   13 GDSDLQLERINVYYNEA 29

The following BLAST results are available for this feature:

BLAST of CF504111 vs. ExPASy Swiss-Prot

BLAST of CF504111 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 219


Match Name	E-value	Identity	Description
TBB_AJECA	9.519e-16	65.79	Tubulin beta chain OS=Ajellomyces capsulata GN=TUB...	[more]
TBB_CANAL	4.653e-15	64.10	Tubulin beta chain OS=Candida albicans GN=TUB2 PE=...	[more]
TBB1_TRIVI	7.484e-15	60.00	Tubulin beta-1 chain OS=Trichoderma viride GN=tub1...	[more]
TBB_YEAST	8.282e-14	68.42	Tubulin beta chain OS=Saccharomyces cerevisiae GN=...	[more]
TBB_ASPFU	1.269e-13	61.76	Tubulin beta chain OS=Aspergillus fumigatus GN=AFU...	[more]
TBB1_COLGR	3.065e-13	60.53	Tubulin beta-1 chain OS=Colletotrichum graminicola...	[more]
TBB1_CHOCR	1.909e-12	64.10	Tubulin beta-1 chain OS=Chondrus crispus GN=TUBB P...	[more]
TBB1_COLGL	5.427e-12	54.76	Tubulin beta-1 chain OS=Colletotrichum gloeosporio...	[more]
TBB2_OOMCK	3.593e-11	90.48	Tubulin beta-2 chain (Fragment) OS=Oomycete-like s...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_119000-599 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-14_A02 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF504111 ID=CF504111; Name=CF504111; organism=Citrus sinensis; type=EST; length=256bp
CTTCGTTTCCTTTCCGTTTGAGATAAATGAGAGAGATCTTGCACATTCAA
GGAGGCCAATGCGGCAACCAAATCGGATCCAAATTCTGGGAAGTCATTTG
CGACGAGCATGGCGTGGACCCCACGGGGAAGTATAGAGGTGACCGGGGTT
GAGGATCTTCAACTGGAGCGGATCAATGTCTACTACAACCGAAGCTTCCG
GTGGAAGGTACGTGCCTCGGGCTGTTCTCATGGATCTTGAACCCGGTACC
ATGGGA