CF504131 | Citrus Genome Database

Overview


Name	CF504131
Unique Name	CF504131
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	342

Libraries


Library Name	Type
Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
CF504131_ssr283	microsatellite	CF504131_ssr283:283..298.	BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig10947	contig	Csv1_Contig10947:167..505.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CF504131 vs. ExPASy Swiss-Prot
Match: METK1_DAUCA (S-adenosylmethionine synthetase 1 OS=Daucus carota GN=SAMS1 PE=2 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 1.980e-23
Identity = 29/31 (93.55%), Postives = 30/31 (96.77%), Query Frame = 2

Query:   92 GRFLKTAAYGHFGRDDPDFTWEIVKPLK*EK 184
            GRFLKTAAYGHFGRDDPDFTWE+VKPLK EK
Sbjct:  408 GRFLKTAAYGHFGRDDPDFTWEVVKPLKWEK 438

HSP 2 Score: 61.6178 bits (148), Expect = 1.980e-23
Identity = 29/35 (82.86%), Postives = 32/35 (91.43%), Query Frame = 1

Query:    1 DKEILKIVKENFDFRPGMISINLDLKRGGNRQVLE 105
            D+EILKIVKE FDFRPGMISINLDLKRGGN + L+
Sbjct:  378 DREILKIVKETFDFRPGMISINLDLKRGGNGRFLK 412

[Close]

BLAST of CF504131 vs. ExPASy Swiss-Prot
Match: METK_PHALU (S-adenosylmethionine synthetase OS=Phaseolus lunatus GN=SAMS PE=2 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 1.984e-23
Identity = 31/35 (88.57%), Postives = 33/35 (94.29%), Query Frame = 1

Query:    1 DKEILKIVKENFDFRPGMISINLDLKRGGNRQVLE 105
            DKEILKIVKENFDFRPGMISINLDLKRGGN + L+
Sbjct:  331 DKEILKIVKENFDFRPGMISINLDLKRGGNSRFLK 365

HSP 2 Score: 63.5438 bits (153), Expect = 1.984e-23
Identity = 27/30 (90.00%), Postives = 29/30 (96.67%), Query Frame = 2

Query:   95 RFLKTAAYGHFGRDDPDFTWEIVKPLK*EK 184
            RFLKTAAYGHFGR+DPDFTWE+VKPLK EK
Sbjct:  362 RFLKTAAYGHFGREDPDFTWEVVKPLKWEK 391

[Close]

BLAST of CF504131 vs. ExPASy Swiss-Prot
Match: METK2_SUASA (S-adenosylmethionine synthetase 2 OS=Suaeda salsa GN=METK2 PE=2 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 2.582e-23
Identity = 29/33 (87.88%), Postives = 30/33 (90.91%), Query Frame = 2

Query:   95 RFLKTAAYGHFGRDDPDFTWEIVKPLK*EKLPA 193
            RF KTAAYGHFGRDDPDFTWE VKPLK EK+PA
Sbjct:  361 RFQKTAAYGHFGRDDPDFTWETVKPLKWEKVPA 393

HSP 2 Score: 62.7734 bits (151), Expect = 2.582e-23
Identity = 29/30 (96.67%), Postives = 30/30 (100.00%), Query Frame = 1

Query:    1 DKEILKIVKENFDFRPGMISINLDLKRGGN 90
            DK+ILKIVKENFDFRPGMISINLDLKRGGN
Sbjct:  330 DKDILKIVKENFDFRPGMISINLDLKRGGN 359

[Close]

BLAST of CF504131 vs. ExPASy Swiss-Prot
Match: METK1_POPTR (S-adenosylmethionine synthetase 1 OS=Populus trichocarpa GN=METK1 PE=2 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 2.582e-23
Identity = 28/30 (93.33%), Postives = 29/30 (96.67%), Query Frame = 2

Query:   95 RFLKTAAYGHFGRDDPDFTWEIVKPLK*EK 184
            RFLKTAAYGHFGRDDPDFTWE+VKPLK EK
Sbjct:  360 RFLKTAAYGHFGRDDPDFTWEVVKPLKWEK 389

HSP 2 Score: 63.5438 bits (153), Expect = 2.582e-23
Identity = 29/31 (93.55%), Postives = 31/31 (100.00%), Query Frame = 1

Query:    1 DKEILKIVKENFDFRPGMISINLDLKRGGNR 93
            DKEILKIVKENFDFRPGM++INLDLKRGGNR
Sbjct:  330 DKEILKIVKENFDFRPGMMTINLDLKRGGNR 360

[Close]

BLAST of CF504131 vs. ExPASy Swiss-Prot
Match: METK1_ELAUM (S-adenosylmethionine synthetase 1 OS=Elaeagnus umbellata GN=SAMS1 PE=2 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 3.359e-23
Identity = 29/31 (93.55%), Postives = 29/31 (93.55%), Query Frame = 2

Query:   92 GRFLKTAAYGHFGRDDPDFTWEIVKPLK*EK 184
            GRFLKTA YGHFGRDDPDFTWEIVKPLK EK
Sbjct:  360 GRFLKTAGYGHFGRDDPDFTWEIVKPLKWEK 390

HSP 2 Score: 62.003 bits (149), Expect = 3.359e-23
Identity = 29/35 (82.86%), Postives = 32/35 (91.43%), Query Frame = 1

Query:    1 DKEILKIVKENFDFRPGMISINLDLKRGGNRQVLE 105
            DKEIL IVKENFDFRPGMI+INLDLKRGGN + L+
Sbjct:  330 DKEILNIVKENFDFRPGMITINLDLKRGGNGRFLK 364

[Close]

BLAST of CF504131 vs. ExPASy Swiss-Prot
Match: METK3_PETHY (S-adenosylmethionine synthetase 3 OS=Petunia hybrida GN=SAM3 PE=2 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 3.359e-23
Identity = 28/31 (90.32%), Postives = 29/31 (93.55%), Query Frame = 2

Query:   92 GRFLKTAAYGHFGRDDPDFTWEIVKPLK*EK 184
            GRFLKTAAYGHFGRDD DFTWE+VKPLK EK
Sbjct:  360 GRFLKTAAYGHFGRDDTDFTWEVVKPLKCEK 390

HSP 2 Score: 63.929 bits (154), Expect = 3.359e-23
Identity = 30/35 (85.71%), Postives = 33/35 (94.29%), Query Frame = 1

Query:    1 DKEILKIVKENFDFRPGMISINLDLKRGGNRQVLE 105
            DKEILKIVKENFDFRPGMI+INLDLKRGGN + L+
Sbjct:  330 DKEILKIVKENFDFRPGMIAINLDLKRGGNGRFLK 364

[Close]

BLAST of CF504131 vs. ExPASy Swiss-Prot
Match: METK1_ORYSJ (S-adenosylmethionine synthetase 1 OS=Oryza sativa subsp. japonica GN=SAM1 PE=2 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 4.370e-23
Identity = 28/31 (90.32%), Postives = 30/31 (96.77%), Query Frame = 2

Query:   92 GRFLKTAAYGHFGRDDPDFTWEIVKPLK*EK 184
            GR+LKTAAYGHFGRDDPDFTWE+VKPLK EK
Sbjct:  363 GRYLKTAAYGHFGRDDPDFTWEVVKPLKWEK 393

HSP 2 Score: 61.6178 bits (148), Expect = 4.370e-23
Identity = 29/35 (82.86%), Postives = 32/35 (91.43%), Query Frame = 1

Query:    1 DKEILKIVKENFDFRPGMISINLDLKRGGNRQVLE 105
            DKEILKIVKENFDFRPGMI INLDLK+GGN + L+
Sbjct:  333 DKEILKIVKENFDFRPGMIIINLDLKKGGNGRYLK 367

[Close]

BLAST of CF504131 vs. ExPASy Swiss-Prot
Match: METK1_ORYSI (S-adenosylmethionine synthetase 1 OS=Oryza sativa subsp. indica GN=SAM1 PE=2 SV=2)

HSP 1 Score: 66.2402 bits (160), Expect = 4.370e-23
Identity = 28/31 (90.32%), Postives = 30/31 (96.77%), Query Frame = 2

Query:   92 GRFLKTAAYGHFGRDDPDFTWEIVKPLK*EK 184
            GR+LKTAAYGHFGRDDPDFTWE+VKPLK EK
Sbjct:  363 GRYLKTAAYGHFGRDDPDFTWEVVKPLKWEK 393

HSP 2 Score: 61.6178 bits (148), Expect = 4.370e-23
Identity = 29/35 (82.86%), Postives = 32/35 (91.43%), Query Frame = 1

Query:    1 DKEILKIVKENFDFRPGMISINLDLKRGGNRQVLE 105
            DKEILKIVKENFDFRPGMI INLDLK+GGN + L+
Sbjct:  333 DKEILKIVKENFDFRPGMIIINLDLKKGGNGRYLK 367

[Close]

BLAST of CF504131 vs. ExPASy Swiss-Prot
Match: METK_DENCR (S-adenosylmethionine synthetase OS=Dendrobium crumenatum GN=SAMS PE=2 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 4.371e-23
Identity = 30/31 (96.77%), Postives = 31/31 (100.00%), Query Frame = 1

Query:    1 DKEILKIVKENFDFRPGMISINLDLKRGGNR 93
            DKEILKIVKENFDFRPGMI+INLDLKRGGNR
Sbjct:  333 DKEILKIVKENFDFRPGMITINLDLKRGGNR 363

HSP 2 Score: 63.1586 bits (152), Expect = 4.371e-23
Identity = 26/30 (86.67%), Postives = 29/30 (96.67%), Query Frame = 2

Query:   95 RFLKTAAYGHFGRDDPDFTWEIVKPLK*EK 184
            RF+KTAAYGHFGRDDPDFTWE+VKPLK +K
Sbjct:  363 RFIKTAAYGHFGRDDPDFTWEVVKPLKWDK 392

[Close]

BLAST of CF504131 vs. ExPASy Swiss-Prot
Match: METK1_PETCR (S-adenosylmethionine synthetase 1 (Fragment) OS=Petroselinum crispum GN=SMS-1 PE=2 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 5.753e-23
Identity = 28/31 (90.32%), Postives = 30/31 (96.77%), Query Frame = 2

Query:   92 GRFLKTAAYGHFGRDDPDFTWEIVKPLK*EK 184
            GRFLKTAAYGHFGR+DPDFTWE+VKPLK EK
Sbjct:  203 GRFLKTAAYGHFGREDPDFTWEVVKPLKWEK 233

HSP 2 Score: 61.6178 bits (148), Expect = 5.753e-23
Identity = 29/35 (82.86%), Postives = 32/35 (91.43%), Query Frame = 1

Query:    1 DKEILKIVKENFDFRPGMISINLDLKRGGNRQVLE 105
            D+EILKIVKE FDFRPGMISINLDLKRGGN + L+
Sbjct:  173 DREILKIVKETFDFRPGMISINLDLKRGGNGRFLK 207

The following BLAST results are available for this feature:

BLAST of CF504131 vs. ExPASy Swiss-Prot

BLAST of CF504131 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 79


Match Name	E-value	Identity	Description
METK1_DAUCA	1.980e-23	93.55	S-adenosylmethionine synthetase 1 OS=Daucus carota...	[more]
METK_PHALU	1.984e-23	88.57	S-adenosylmethionine synthetase OS=Phaseolus lunat...	[more]
METK2_SUASA	2.582e-23	87.88	S-adenosylmethionine synthetase 2 OS=Suaeda salsa ...	[more]
METK1_POPTR	2.582e-23	93.33	S-adenosylmethionine synthetase 1 OS=Populus trich...	[more]
METK1_ELAUM	3.359e-23	93.55	S-adenosylmethionine synthetase 1 OS=Elaeagnus umb...	[more]
METK3_PETHY	3.359e-23	90.32	S-adenosylmethionine synthetase 3 OS=Petunia hybri...	[more]
METK1_ORYSJ	4.370e-23	90.32	S-adenosylmethionine synthetase 1 OS=Oryza sativa ...	[more]
METK1_ORYSI	4.370e-23	90.32	S-adenosylmethionine synthetase 1 OS=Oryza sativa ...	[more]
METK_DENCR	4.371e-23	96.77	S-adenosylmethionine synthetase OS=Dendrobium crum...	[more]
METK1_PETCR	5.753e-23	90.32	S-adenosylmethionine synthetase 1 (Fragment) OS=Pe...	[more]

Pages

Properties


Property Name	Value
Genbank description	USDA-FP_119000-619 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-14_B11 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF504131 ID=CF504131; Name=CF504131; organism=Citrus sinensis; type=EST; length=342bp
GACAAGGAAATTTTGAAGATTGTGAAGGAGAATTTTGATTTTAGGCCTGG
GATGATTTCTATTAATCTTGATCTCAAGAGGGGTGGCAACCGGCAGGTTC
TTGAAGACTGCCGCTTATGGGCACTTTGGCAGAGATGATCCTGATTTCAC
TTGGGAGATTGTTAAGCCACTCAAGTAGGAGAAACTTCCAGCTTAAACTA
ATCAAGGATGAGCTGCTGGCCTCTCATTGGTTCTTATATATGCTTACTTT
TTTTTTTACTTTTGTTTCAGTTACATGAATGTTTTCTTTCTTTCTTTCTT
TTTCTTTTCCTTTTTTTTTTTTTTTATAAATATGTTTAATGG