CF504131 | Citrus Genome Database

Overview


Name	CF504131
Unique Name	CF504131
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	342

Libraries


Library Name	Type
Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
CF504131_ssr283	microsatellite	CF504131_ssr283:283..298.	BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig10947	contig	Csv1_Contig10947:167..505.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

BLAST of CF504131 vs. ExPASy Swiss-Prot
Match: METK_BRARP (S-adenosylmethionine synthetase OS=Brassica rapa subsp. pekinensis GN=SAMS PE=2 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 7.400e-23
Identity = 28/31 (90.32%), Postives = 30/31 (96.77%), Query Frame = 2

Query:   92 GRFLKTAAYGHFGRDDPDFTWEIVKPLK*EK 184
            GRFLKTAAYGHFGRDDPDFTWE+VKPLK +K
Sbjct:  360 GRFLKTAAYGHFGRDDPDFTWEVVKPLKWDK 390

HSP 2 Score: 60.8474 bits (146), Expect = 7.400e-23
Identity = 28/35 (80.00%), Postives = 33/35 (94.29%), Query Frame = 1

Query:    1 DKEILKIVKENFDFRPGMISINLDLKRGGNRQVLE 105
            DKEILKIVKE+FDFRPGM++INLDLKRGGN + L+
Sbjct:  330 DKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLK 364

BLAST of CF504131 vs. ExPASy Swiss-Prot
Match: METK5_BRAJU (S-adenosylmethionine synthetase 5 OS=Brassica juncea GN=MSAMS5 PE=2 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 7.400e-23
Identity = 28/31 (90.32%), Postives = 30/31 (96.77%), Query Frame = 2

Query:   92 GRFLKTAAYGHFGRDDPDFTWEIVKPLK*EK 184
            GRFLKTAAYGHFGRDDPDFTWE+VKPLK +K
Sbjct:  360 GRFLKTAAYGHFGRDDPDFTWEVVKPLKWDK 390

HSP 2 Score: 60.8474 bits (146), Expect = 7.400e-23
Identity = 28/35 (80.00%), Postives = 33/35 (94.29%), Query Frame = 1

Query:    1 DKEILKIVKENFDFRPGMISINLDLKRGGNRQVLE 105
            DKEILKIVKE+FDFRPGM++INLDLKRGGN + L+
Sbjct:  330 DKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLK 364

BLAST of CF504131 vs. ExPASy Swiss-Prot
Match: METK3_BRAJU (S-adenosylmethionine synthetase 3 OS=Brassica juncea GN=MSAMS3 PE=2 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 7.400e-23
Identity = 28/31 (90.32%), Postives = 30/31 (96.77%), Query Frame = 2

Query:   92 GRFLKTAAYGHFGRDDPDFTWEIVKPLK*EK 184
            GRFLKTAAYGHFGRDDPDFTWE+VKPLK +K
Sbjct:  360 GRFLKTAAYGHFGRDDPDFTWEVVKPLKWDK 390

HSP 2 Score: 60.8474 bits (146), Expect = 7.400e-23
Identity = 28/35 (80.00%), Postives = 33/35 (94.29%), Query Frame = 1

Query:    1 DKEILKIVKENFDFRPGMISINLDLKRGGNRQVLE 105
            DKEILKIVKE+FDFRPGM++INLDLKRGGN + L+
Sbjct:  330 DKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLK 364

BLAST of CF504131 vs. ExPASy Swiss-Prot
Match: METK1_BRAJU (S-adenosylmethionine synthetase 1 OS=Brassica juncea GN=SAMS1 PE=2 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 7.400e-23
Identity = 28/31 (90.32%), Postives = 30/31 (96.77%), Query Frame = 2

Query:   92 GRFLKTAAYGHFGRDDPDFTWEIVKPLK*EK 184
            GRFLKTAAYGHFGRDDPDFTWE+VKPLK +K
Sbjct:  360 GRFLKTAAYGHFGRDDPDFTWEVVKPLKWDK 390

HSP 2 Score: 60.8474 bits (146), Expect = 7.400e-23
Identity = 28/35 (80.00%), Postives = 33/35 (94.29%), Query Frame = 1

Query:    1 DKEILKIVKENFDFRPGMISINLDLKRGGNRQVLE 105
            DKEILKIVKE+FDFRPGM++INLDLKRGGN + L+
Sbjct:  330 DKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLK 364

BLAST of CF504131 vs. ExPASy Swiss-Prot
Match: METK1_ARATH (S-adenosylmethionine synthetase 1 OS=Arabidopsis thaliana GN=SAM1 PE=1 SV=2)

HSP 1 Score: 66.2402 bits (160), Expect = 7.400e-23
Identity = 28/31 (90.32%), Postives = 30/31 (96.77%), Query Frame = 2

Query:   92 GRFLKTAAYGHFGRDDPDFTWEIVKPLK*EK 184
            GRFLKTAAYGHFGRDDPDFTWE+VKPLK +K
Sbjct:  360 GRFLKTAAYGHFGRDDPDFTWEVVKPLKWDK 390

HSP 2 Score: 60.8474 bits (146), Expect = 7.400e-23
Identity = 28/35 (80.00%), Postives = 33/35 (94.29%), Query Frame = 1

Query:    1 DKEILKIVKENFDFRPGMISINLDLKRGGNRQVLE 105
            DKEILKIVKE+FDFRPGM++INLDLKRGGN + L+
Sbjct:  330 DKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLK 364

BLAST of CF504131 vs. ExPASy Swiss-Prot
Match: METK2_SOLLC (S-adenosylmethionine synthetase 2 OS=Solanum lycopersicum GN=SAM2 PE=2 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 9.629e-23
Identity = 28/31 (90.32%), Postives = 30/31 (96.77%), Query Frame = 2

Query:   92 GRFLKTAAYGHFGRDDPDFTWEIVKPLK*EK 184
            GRFLKTAAYGHFGRDDPDFTWE+VKPLK +K
Sbjct:  360 GRFLKTAAYGHFGRDDPDFTWEVVKPLKWDK 390

HSP 2 Score: 60.4622 bits (145), Expect = 9.629e-23
Identity = 29/35 (82.86%), Postives = 31/35 (88.57%), Query Frame = 1

Query:    1 DKEILKIVKENFDFRPGMISINLDLKRGGNRQVLE 105
            DKEIL IVKENFDFRPGMISINLDL RGGN + L+
Sbjct:  330 DKEILNIVKENFDFRPGMISINLDLLRGGNGRFLK 364

BLAST of CF504131 vs. ExPASy Swiss-Prot
Match: METK_MEDTR (S-adenosylmethionine synthetase OS=Medicago truncatula GN=METK PE=3 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 9.629e-23
Identity = 28/30 (93.33%), Postives = 29/30 (96.67%), Query Frame = 2

Query:   95 RFLKTAAYGHFGRDDPDFTWEIVKPLK*EK 184
            RFLKTAAYGHFGRDDPDFTWE+VKPLK EK
Sbjct:  362 RFLKTAAYGHFGRDDPDFTWEVVKPLKWEK 391

HSP 2 Score: 61.6178 bits (148), Expect = 9.629e-23
Identity = 28/35 (80.00%), Postives = 33/35 (94.29%), Query Frame = 1

Query:    1 DKEILKIVKENFDFRPGMISINLDLKRGGNRQVLE 105
            D+EIL+IVKENFDFRPGMI+INLDLKRGGN + L+
Sbjct:  331 DREILQIVKENFDFRPGMITINLDLKRGGNSRFLK 365

BLAST of CF504131 vs. ExPASy Swiss-Prot
Match: METK2_ORYSJ (S-adenosylmethionine synthetase 2 OS=Oryza sativa subsp. japonica GN=SAM2 PE=2 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 1.253e-22
Identity = 29/31 (93.55%), Postives = 31/31 (100.00%), Query Frame = 1

Query:    1 DKEILKIVKENFDFRPGMISINLDLKRGGNR 93
            DKEILKIVKENFDFRPGM++INLDLKRGGNR
Sbjct:  332 DKEILKIVKENFDFRPGMMTINLDLKRGGNR 362

HSP 2 Score: 62.7734 bits (151), Expect = 1.253e-22
Identity = 26/30 (86.67%), Postives = 29/30 (96.67%), Query Frame = 2

Query:   95 RFLKTAAYGHFGRDDPDFTWEIVKPLK*EK 184
            RF+KTAAYGHFGR+DPDFTWE+VKPLK EK
Sbjct:  362 RFIKTAAYGHFGREDPDFTWEVVKPLKYEK 391

BLAST of CF504131 vs. ExPASy Swiss-Prot
Match: METK2_PETCR (S-adenosylmethionine synthetase 2 (Fragment) OS=Petroselinum crispum GN=SMS-2 PE=2 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 1.678e-22
Identity = 28/31 (90.32%), Postives = 30/31 (96.77%), Query Frame = 2

Query:   92 GRFLKTAAYGHFGRDDPDFTWEIVKPLK*EK 184
            GRFLKTAAYGHFGR+DPDFTWE+VKPLK EK
Sbjct:  114 GRFLKTAAYGHFGREDPDFTWEVVKPLKWEK 144

HSP 2 Score: 60.077 bits (144), Expect = 1.678e-22
Identity = 28/35 (80.00%), Postives = 32/35 (91.43%), Query Frame = 1

Query:    1 DKEILKIVKENFDFRPGMISINLDLKRGGNRQVLE 105
            D+EIL+IVKE FDFRPGMISINLDLKRGGN + L+
Sbjct:   84 DREILQIVKETFDFRPGMISINLDLKRGGNGRFLK 118

BLAST of CF504131 vs. ExPASy Swiss-Prot
Match: METK4_ARATH (S-adenosylmethionine synthetase 4 OS=Arabidopsis thaliana GN=METK4 PE=1 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 2.121e-22
Identity = 28/34 (82.35%), Postives = 30/34 (88.24%), Query Frame = 2

Query:   92 GRFLKTAAYGHFGRDDPDFTWEIVKPLK*EKLPA 193
            GRFLKTAAYGHFGRDD DFTWE+VKPLK  K+ A
Sbjct:  360 GRFLKTAAYGHFGRDDADFTWEVVKPLKSNKVQA 393

HSP 2 Score: 61.6178 bits (148), Expect = 2.121e-22
Identity = 29/35 (82.86%), Postives = 33/35 (94.29%), Query Frame = 1

Query:    1 DKEILKIVKENFDFRPGMISINLDLKRGGNRQVLE 105
            DKEIL+IVKE+FDFRPGMISINLDLKRGGN + L+
Sbjct:  330 DKEILEIVKESFDFRPGMISINLDLKRGGNGRFLK 364

The following BLAST results are available for this feature:

BLAST of CF504131 vs. ExPASy Swiss-Prot

BLAST of CF504131 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 79


Match Name	E-value	Identity	Description
METK_BRARP	7.400e-23	90.32	S-adenosylmethionine synthetase OS=Brassica rapa s...	[more]
METK5_BRAJU	7.400e-23	90.32	S-adenosylmethionine synthetase 5 OS=Brassica junc...	[more]
METK3_BRAJU	7.400e-23	90.32	S-adenosylmethionine synthetase 3 OS=Brassica junc...	[more]
METK1_BRAJU	7.400e-23	90.32	S-adenosylmethionine synthetase 1 OS=Brassica junc...	[more]
METK1_ARATH	7.400e-23	90.32	S-adenosylmethionine synthetase 1 OS=Arabidopsis t...	[more]
METK2_SOLLC	9.629e-23	90.32	S-adenosylmethionine synthetase 2 OS=Solanum lycop...	[more]
METK_MEDTR	9.629e-23	93.33	S-adenosylmethionine synthetase OS=Medicago trunca...	[more]
METK2_ORYSJ	1.253e-22	93.55	S-adenosylmethionine synthetase 2 OS=Oryza sativa ...	[more]
METK2_PETCR	1.678e-22	90.32	S-adenosylmethionine synthetase 2 (Fragment) OS=Pe...	[more]
METK4_ARATH	2.121e-22	82.35	S-adenosylmethionine synthetase 4 OS=Arabidopsis t...	[more]

Pages

Properties


Property Name	Value
Genbank description	USDA-FP_119000-619 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-14_B11 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF504131 ID=CF504131; Name=CF504131; organism=Citrus sinensis; type=EST; length=342bp
GACAAGGAAATTTTGAAGATTGTGAAGGAGAATTTTGATTTTAGGCCTGG
GATGATTTCTATTAATCTTGATCTCAAGAGGGGTGGCAACCGGCAGGTTC
TTGAAGACTGCCGCTTATGGGCACTTTGGCAGAGATGATCCTGATTTCAC
TTGGGAGATTGTTAAGCCACTCAAGTAGGAGAAACTTCCAGCTTAAACTA
ATCAAGGATGAGCTGCTGGCCTCTCATTGGTTCTTATATATGCTTACTTT
TTTTTTTACTTTTGTTTCAGTTACATGAATGTTTTCTTTCTTTCTTTCTT
TTTCTTTTCCTTTTTTTTTTTTTTTATAAATATGTTTAATGG