CF504216

Overview
NameCF504216
Unique NameCF504216
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length437
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CF504216 vs. ExPASy Swiss-Prot
Match: METE_PSEE4 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Pseudomonas entomophila (strain L48) GN=metE PE=3 SV=1)

HSP 1 Score: 127.102 bits (318), Expect = 3.005e-29
Identity = 66/110 (60.00%), Postives = 76/110 (69.09%), Query Frame = 1 
Query:    1 CYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLR 330
            CYS FND+I SI  MDADVITIE SRSD +LL  F E   Y   IGPGVYDIHSPR+P+ EE+   + K    +    LWVNPDCGLKTR + E + AL NMVAAA+ LR
Sbjct:  651 CYSEFNDVIESIAAMDADVITIETSRSDMELLEAF-ERFDYPNEIGPGVYDIHSPRVPTKEEMVKLLRKAAQRIPAERLWVNPDCGLKTRAWPETEAALVNMVAAARELR 759
[Close]
BLAST of CF504216 vs. ExPASy Swiss-Prot
Match: METE_PSEU5 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Pseudomonas stutzeri (strain A1501) GN=metE PE=3 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 3.924e-29
Identity = 65/110 (59.09%), Postives = 74/110 (67.27%), Query Frame = 1 
Query:    1 CYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLR 330
            CYS FND+I SI  MDADVITIE SRS  +LL  FR    Y   IGPGVYDIHSPR+P T E+   + K    +    LWVNPDCGLKTR + E + AL NMVAAA+ LR
Sbjct:  655 CYSEFNDVIESIAAMDADVITIETSRSQMELLEAFR-AFDYPNDIGPGVYDIHSPRVPDTAEMVQLLEKAAECIPAERLWVNPDCGLKTRGWPETEAALVNMVAAARQLR 763
[Close]
BLAST of CF504216 vs. ExPASy Swiss-Prot
Match: METE_VIBHA (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Vibrio harveyi GN=metE PE=3 SV=1)

HSP 1 Score: 126.331 bits (316), Expect = 5.125e-29
Identity = 65/113 (57.52%), Postives = 79/113 (69.91%), Query Frame = 1 
Query:    1 CYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQL 339
            CYS FN+II S+  +DADVITIE SRS+ +LL  F E   Y   IGPGVYDIHSP IPS E I + I K    +    LWVNPDCGLKTR ++E + AL+N+V+AAK LR +L
Sbjct:  648 CYSEFNEIIDSVAALDADVITIETSRSNMELLKAF-EDFNYPNEIGPGVYDIHSPNIPSEEWIEELIKKAAEKIPAQRLWVNPDCGLKTRNWSETEAALANLVSAAKKLRAEL 759
[Close]
BLAST of CF504216 vs. ExPASy Swiss-Prot
Match: METE_STAES (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Staphylococcus epidermidis (strain ATCC 12228) GN=metE PE=3 SV=1)

HSP 1 Score: 125.946 bits (315), Expect = 6.694e-29
Identity = 65/115 (56.52%), Postives = 79/115 (68.70%), Query Frame = 1 
Query:    1 CYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQLAS 345
            CYS F  IIH+I D+DADVI+IE SRS   L+  F E + Y  GIG GVYDIHSPRIP+ EEI   IN+ L  ++ ++ WVNPDCGLKTRK  EVK AL+ +V A K  R +  S
Sbjct:  632 CYSQFGQIIHAIHDLDADVISIETSRSHGDLIQDF-EDINYDLGIGLGVYDIHSPRIPTEEEITTAINRSLQQIDRSLFWVNPDCGLKTRKENEVKDALTVLVNAVKKKRQESES 745
[Close]
BLAST of CF504216 vs. ExPASy Swiss-Prot
Match: METE_STAEQ (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) GN=metE PE=3 SV=1)

HSP 1 Score: 125.946 bits (315), Expect = 6.694e-29
Identity = 65/115 (56.52%), Postives = 79/115 (68.70%), Query Frame = 1 
Query:    1 CYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQLAS 345
            CYS F  IIH+I D+DADVI+IE SRS   L+  F E + Y  GIG GVYDIHSPRIP+ EEI   IN+ L  ++ ++ WVNPDCGLKTRK  EVK AL+ +V A K  R +  S
Sbjct:  632 CYSQFGQIIHAIHDLDADVISIETSRSHGDLIQDF-EDINYDLGIGLGVYDIHSPRIPTEEEITTAINRSLQQIDRSLFWVNPDCGLKTRKENEVKDALTVLVNAVKKKRQESES 745
[Close]
BLAST of CF504216 vs. ExPASy Swiss-Prot
Match: METE_RUTMC (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Ruthia magnifica subsp. Calyptogena magnifica GN=metE PE=3 SV=1)

HSP 1 Score: 125.946 bits (315), Expect = 6.694e-29
Identity = 65/110 (59.09%), Postives = 76/110 (69.09%), Query Frame = 1 
Query:    1 CYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLR 330
            CYS FNDII +I  MDADVITIE SRSD +LL +F E   Y   IGPGVYDIHSP IPS + I + + K    +    LWVNPDCGLKTR + EV  AL+NMV A++ LR
Sbjct:  648 CYSEFNDIIEAIAQMDADVITIETSRSDMELLDIFDE-FDYPNEIGPGVYDIHSPNIPSVDSIVELMQKAAKYIPIKRLWVNPDCGLKTRHWDEVNLALTNMVLASQQLR 756
[Close]
BLAST of CF504216 vs. ExPASy Swiss-Prot
Match: METE_VIBPA (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Vibrio parahaemolyticus GN=metE PE=3 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 8.743e-29
Identity = 65/114 (57.02%), Postives = 78/114 (68.42%), Query Frame = 1 
Query:    1 CYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQLA 342
            CYS FN+II S+  +DADVITIE SRS+ +LL  F E   Y   IGPGVYDIHSP IP+ E I   I K    +    LWVNPDCGLKTR + E + AL+N+V+AAK LR +LA
Sbjct:  648 CYSEFNEIIDSVAALDADVITIETSRSNMELLKAFEE-FNYPNEIGPGVYDIHSPNIPTEEWIEGLIKKAAEKIPVQRLWVNPDCGLKTRNWAETEAALANLVSAAKKLRAELA 760
[Close]
BLAST of CF504216 vs. ExPASy Swiss-Prot
Match: METE_VIBHB (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Vibrio harveyi (strain ATCC BAA-1116 / BB120) GN=metE PE=3 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 8.743e-29
Identity = 65/114 (57.02%), Postives = 79/114 (69.30%), Query Frame = 1 
Query:    1 CYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQLA 342
            CYS FN+II S+  +DADVITIE SRS+ +LL  F E   Y   IGPGVYDIHSP IP+ E I   + K    +    LWVNPDCGLKTR + E + AL+N+V+AAK LRT+LA
Sbjct:  648 CYSEFNEIIDSVAALDADVITIETSRSNMELLKAF-EAFNYPNEIGPGVYDIHSPNIPTQEWIEALLKKAAEKVPVQRLWVNPDCGLKTRNWDETEAALANLVSAAKKLRTELA 760
[Close]
BLAST of CF504216 vs. ExPASy Swiss-Prot
Match: METE_STAAW (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Staphylococcus aureus (strain MW2) GN=metE PE=3 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 8.743e-29
Identity = 64/112 (57.14%), Postives = 78/112 (69.64%), Query Frame = 1 
Query:    1 CYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQ 336
            CYS F  IIH+I D+DADVI+IE SRS   L+  F E + Y  GIG GVYDIHSPRIP+ EEI   IN+ L  ++ ++ WVNPDCGLKTRK  EVK AL+ +V A K  R +
Sbjct:  632 CYSQFGQIIHAIHDLDADVISIETSRSHGDLIKDF-EDINYDLGIGLGVYDIHSPRIPTKEEITTAINRSLQQIDRSLFWVNPDCGLKTRKEEEVKDALTVLVNAVKAKRQE 742
[Close]
BLAST of CF504216 vs. ExPASy Swiss-Prot
Match: METE_STAAT (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Staphylococcus aureus (strain USA300 / TCH1516) GN=metE PE=3 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 8.743e-29
Identity = 64/112 (57.14%), Postives = 78/112 (69.64%), Query Frame = 1 
Query:    1 CYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQ 336
            CYS F  IIH+I D+DADVI+IE SRS   L+  F E + Y  GIG GVYDIHSPRIP+ EEI   IN+ L  ++ ++ WVNPDCGLKTRK  EVK AL+ +V A K  R +
Sbjct:  632 CYSQFGQIIHAIHDLDADVISIETSRSHGDLIKDF-EDINYDLGIGLGVYDIHSPRIPTKEEITTAINRSLQQIDRSLFWVNPDCGLKTRKEEEVKDALTVLVNAVKAKRQE 742
The following BLAST results are available for this feature:
BLAST of CF504216 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 269
Match NameE-valueIdentityDescription
METE_PSEE43.005e-2960.005-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_PSEU53.924e-2959.095-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_VIBHA5.125e-2957.525-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_STAES6.694e-2956.525-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_STAEQ6.694e-2956.525-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_RUTMC6.694e-2959.095-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_VIBPA8.743e-2957.025-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_VIBHB8.743e-2957.025-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_STAAW8.743e-2957.145-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_STAAT8.743e-2957.145-methyltetrahydropteroyltriglutamate--homocystein... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_119000-705 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-15_E03 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF504216 ID=CF504216; Name=CF504216; organism=Citrus sinensis; type=EST; length=437bp
TGCTACTCCAACTTCAACGACATTATCCACTCAATTATCGACATGGATGC
TGATGTGATCACCATTGAGAACTCTCGTTCTGATGAGAAGCTCCTGTCTG
TCTTCCGTGAGGGAGTCAAGTATGGTGCTGGAATTGGCCCTGGTGTGTAT
GACATCCACTCTCCAAGAATACCATCAACTGAAGAAATTGCTGACAGAAT
CAACAAGATGCTTGCTGTGCTAGAAACCAACATTTTGTGGGTTAACCCTG
ACTGTGGTCTTAAGACTCGCAAGTACACTGAGGTGAAGCCAGCCCTCTCA
AACATGGTCGCTGCTGCCAAGCTACTCCGCACCCAGCTTGCCAGTGCCAA
GTGAGTGGTTCTGGAGATTGGAGGGATACTTCCTGAGCCATTGATTTTTT
TTTTTTTCACTTTAAACAAAGAAAGAAAATTCCTGTC
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