CF504285 | Citrus Genome Database

Overview


Name	CF504285
Unique Name	CF504285
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	390

Libraries


Library Name	Type
Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK2_MOUSE (S-adenosylmethionine synthetase isoform type-2 OS=Mus musculus GN=Mat2a PE=2 SV=2)

HSP 1 Score: 92.4337 bits (228), Expect = 3.317e-21
Identity = 40/70 (57.14%), Postives = 55/70 (78.57%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+CDQ+SDAVLDA L QDPD+KVACET  KT M+++ GEIT++A +DY+K+VR+  + IG+  +  G D
Sbjct:   31 DKICDQISDAVLDAHLQQDPDAKVACETVAKTGMILLAGEITSRAAIDYQKVVREAIKHIGYDDSSKGFD 100

HSP 2 Score: 28.4906 bits (62), Expect = 3.317e-21
Identity = 12/13 (92.31%), Postives = 12/13 (92.31%), Query Frame = 3

Query:  135 TFLFTSESVNEGH 173
            TFLFTSESV EGH
Sbjct:   17 TFLFTSESVGEGH 29

[Close]

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_LACBA (S-adenosylmethionine synthetase OS=Lactobacillus brevis (strain ATCC 367 / JCM 1170) GN=metK PE=3 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 4.314e-21
Identity = 43/70 (61.43%), Postives = 54/70 (77.14%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+ DQ+SDA+LDA LAQDPDS+VACET   T +V+VFGEI+TKA VD +K+VR T + IG+T    G D
Sbjct:   18 DKIADQISDAILDALLAQDPDSRVACETSVTTGLVLVFGEISTKAYVDIQKVVRQTIKEIGYTDGQYGFD 87

HSP 2 Score: 25.7942 bits (55), Expect = 4.314e-21
Identity = 11/14 (78.57%), Postives = 12/14 (85.71%), Query Frame = 3

Query:  132 ETFLFTSESVNEGH 173
            E  LFTSESV+EGH
Sbjct:    3 ERHLFTSESVSEGH 16

[Close]

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK5_CAEEL (Probable S-adenosylmethionine synthetase 5 OS=Caenorhabditis elegans GN=sams-5 PE=2 SV=2)

HSP 1 Score: 92.8189 bits (229), Expect = 7.297e-21
Identity = 43/70 (61.43%), Postives = 55/70 (78.57%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+CDQ+SDAVLDA LAQDP +KVACET TKT M+M+ GEIT+KA VDY+ +VR+  + IG+  +  G D
Sbjct:   19 DKMCDQISDAVLDAHLAQDPHAKVACETVTKTGMIMLCGEITSKAVVDYQVLVRNVIKKIGYDDSSKGFD 88

HSP 2 Score: 26.9498 bits (58), Expect = 7.297e-21
Identity = 11/12 (91.67%), Postives = 12/12 (100.00%), Query Frame = 3

Query:  138 FLFTSESVNEGH 173
            FLFTSESV+EGH
Sbjct:    6 FLFTSESVSEGH 17

[Close]

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK4_CAEEL (Probable S-adenosylmethionine synthetase 4 OS=Caenorhabditis elegans GN=sams-4 PE=2 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 7.297e-21
Identity = 43/70 (61.43%), Postives = 55/70 (78.57%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+CDQ+SDAVLDA LAQDP +KVACET TKT M+M+ GEIT+KA VDY+ +VR+  + IG+  +  G D
Sbjct:   19 DKMCDQISDAVLDAHLAQDPHAKVACETVTKTGMIMLCGEITSKAVVDYQVLVRNVIKKIGYDDSSKGFD 88

HSP 2 Score: 26.9498 bits (58), Expect = 7.297e-21
Identity = 11/12 (91.67%), Postives = 12/12 (100.00%), Query Frame = 3

Query:  138 FLFTSESVNEGH 173
            FLFTSESV+EGH
Sbjct:    6 FLFTSESVSEGH 17

[Close]

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK3_CAEEL (Probable S-adenosylmethionine synthetase 3 OS=Caenorhabditis elegans GN=sams-3 PE=2 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 7.297e-21
Identity = 43/70 (61.43%), Postives = 55/70 (78.57%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+CDQ+SDAVLDA LAQDP +KVACET TKT M+M+ GEIT+KA VDY+ +VR+  + IG+  +  G D
Sbjct:   19 DKMCDQISDAVLDAHLAQDPHAKVACETVTKTGMIMLCGEITSKAVVDYQVLVRNVIKKIGYDDSSKGFD 88

HSP 2 Score: 26.9498 bits (58), Expect = 7.297e-21
Identity = 11/12 (91.67%), Postives = 12/12 (100.00%), Query Frame = 3

Query:  138 FLFTSESVNEGH 173
            FLFTSESV+EGH
Sbjct:    6 FLFTSESVSEGH 17

[Close]

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK2_RAT (S-adenosylmethionine synthetase isoform type-2 OS=Rattus norvegicus GN=Mat2a PE=2 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 7.301e-21
Identity = 39/70 (55.71%), Postives = 55/70 (78.57%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+CDQ++DAVLDA L QDPD+KVACET  KT M+++ GEIT++A +DY+K+VR+  + IG+  +  G D
Sbjct:   31 DKICDQINDAVLDAHLQQDPDAKVACETVAKTGMILLAGEITSRAAIDYQKVVREAIKHIGYDDSSKGFD 100

HSP 2 Score: 28.4906 bits (62), Expect = 7.301e-21
Identity = 12/13 (92.31%), Postives = 12/13 (92.31%), Query Frame = 3

Query:  135 TFLFTSESVNEGH 173
            TFLFTSESV EGH
Sbjct:   17 TFLFTSESVGEGH 29

[Close]

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_PHOLL (S-adenosylmethionine synthetase OS=Photorhabdus luminescens subsp. laumondii GN=metK PE=3 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 7.303e-21
Identity = 44/71 (61.97%), Postives = 54/71 (76.06%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDA 390
            DK+ DQ+SDAVLDA L QDP ++VACET  KT MVMV GEITT A VD E+I R T R IG+ ++++G DA
Sbjct:   17 DKIADQISDAVLDAILEQDPKARVACETYVKTGMVMVGGEITTSAWVDIEEITRQTVREIGYVNSEMGFDA 87

HSP 2 Score: 29.261 bits (64), Expect = 7.303e-21
Identity = 12/15 (80.00%), Postives = 14/15 (93.33%), Query Frame = 3

Query:  129 METFLFTSESVNEGH 173
            M T+LFTSESV+EGH
Sbjct:    1 MTTYLFTSESVSEGH 15

[Close]

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_NITEC (S-adenosylmethionine synthetase OS=Nitrosomonas eutropha (strain C91) GN=metK PE=3 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 2.720e-20
Identity = 40/71 (56.34%), Postives = 54/71 (76.06%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDA 390
            DK+ DQ+SDA+LDA L QDP ++VACET   T ++++ GEITT A +DY  I RDT R IG+TS+++G DA
Sbjct:   17 DKVADQISDAILDAILQQDPHARVACETMCSTGLIVLSGEITTHATIDYNVIPRDTVREIGYTSSEIGFDA 87

HSP 2 Score: 27.7202 bits (60), Expect = 2.720e-20
Identity = 11/15 (73.33%), Postives = 13/15 (86.67%), Query Frame = 3

Query:  129 METFLFTSESVNEGH 173
            M  +LFTSESV+EGH
Sbjct:    1 MSNYLFTSESVSEGH 15

[Close]

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_PROM1 (S-adenosylmethionine synthetase OS=Prochlorococcus marinus (strain NATL1A) GN=metK PE=3 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 3.533e-20
Identity = 43/72 (59.72%), Postives = 56/72 (77.78%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSA-DVGLDA 390
            DK+CDQVSDAVLDACL++DP S+VACE    T + ++ GEIT+KA VD+ K+VR+  +SIG+ SA D G DA
Sbjct:   17 DKICDQVSDAVLDACLSEDPTSRVACEAVVNTGLCLITGEITSKAEVDFNKLVREVIKSIGYRSASDGGFDA 88

HSP 2 Score: 25.7942 bits (55), Expect = 3.533e-20
Identity = 10/15 (66.67%), Postives = 12/15 (80.00%), Query Frame = 3

Query:  129 METFLFTSESVNEGH 173
            M  ++FTSESV EGH
Sbjct:    1 MSRYVFTSESVTEGH 15

[Close]

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_ALTMD (S-adenosylmethionine synthetase OS=Alteromonas macleodii (strain DSM 17117 / Deep ecotype) GN=metK PE=3 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 3.539e-20
Identity = 42/71 (59.15%), Postives = 55/71 (77.46%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDA 390
            DK+ DQ+SDAVLDA L QDP ++VACET  KT MV+V GE+TT A VD E++ R+T + IG+T +D+G DA
Sbjct:   17 DKIADQISDAVLDAILEQDPRARVACETYVKTGMVLVGGEVTTSAWVDIEELTRNTVKEIGYTHSDMGFDA 87

HSP 2 Score: 27.7202 bits (60), Expect = 3.539e-20
Identity = 12/15 (80.00%), Postives = 13/15 (86.67%), Query Frame = 3

Query:  129 METFLFTSESVNEGH 173
            M T LFTSESV+EGH
Sbjct:    1 MATHLFTSESVSEGH 15

The following BLAST results are available for this feature:

BLAST of CF504285 vs. ExPASy Swiss-Prot

BLAST of CF504285 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
METK2_MOUSE	3.317e-21	57.14	S-adenosylmethionine synthetase isoform type-2 OS=...	[more]
METK_LACBA	4.314e-21	61.43	S-adenosylmethionine synthetase OS=Lactobacillus b...	[more]
METK5_CAEEL	7.297e-21	61.43	Probable S-adenosylmethionine synthetase 5 OS=Caen...	[more]
METK4_CAEEL	7.297e-21	61.43	Probable S-adenosylmethionine synthetase 4 OS=Caen...	[more]
METK3_CAEEL	7.297e-21	61.43	Probable S-adenosylmethionine synthetase 3 OS=Caen...	[more]
METK2_RAT	7.301e-21	55.71	S-adenosylmethionine synthetase isoform type-2 OS=...	[more]
METK_PHOLL	7.303e-21	61.97	S-adenosylmethionine synthetase OS=Photorhabdus lu...	[more]
METK_NITEC	2.720e-20	56.34	S-adenosylmethionine synthetase OS=Nitrosomonas eu...	[more]
METK_PROM1	3.533e-20	59.72	S-adenosylmethionine synthetase OS=Prochlorococcus...	[more]
METK_ALTMD	3.539e-20	59.15	S-adenosylmethionine synthetase OS=Alteromonas mac...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_119000-774 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-16_E09 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF504285 ID=CF504285; Name=CF504285; organism=Citrus sinensis; type=EST; length=390bp
CGTCTTTCTCTCGTTCTTCTGGGTACTTACCATTTTCGTATATCACCACC
TTCAATTCCATTTGTACTCGCACCACGGCCTCCTGCAAAGCGAAAGCTGC
AGCTTCAGGTTAAATCAAATTAAAGAAAATGGAAACATTTCTTTTCACAT
CAGAATCAGTGAACGAGGGTCACCTCGGACAAGCTGTGTGACCAAGTTTC
GGACGCAGTACTGGACGCGTGTTTGGCTCAGGATCCTGACAGCAAAGTTG
CTTGTGAAACATGCACAAAGACCAACATGGTCATGGTTTTTGGAGAAATT
ACAACCAAGGCCAATGTGGATTACGAGAAAATCGTGCGTGATACATGCAG
ATCGATTGGGTTCACATCGGCCGATGTGGGGCTTGATGCT