CF504285 | Citrus Genome Database

Overview


Name	CF504285
Unique Name	CF504285
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	390

Libraries


Library Name	Type
Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_PSEPF (S-adenosylmethionine synthetase OS=Pseudomonas fluorescens (strain Pf0-1) GN=metK PE=3 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 1.487e-17
Identity = 41/70 (58.57%), Postives = 52/70 (74.29%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+ DQ+SDAVLDA +AQD  ++VA ET  KT + +V GE+TT A VD E+IVRD    IG+TS+DVG D
Sbjct:   18 DKIADQISDAVLDAIIAQDKHARVAVETLVKTGVAIVAGEVTTSAWVDLEQIVRDVICDIGYTSSDVGFD 87

HSP 2 Score: 25.0238 bits (53), Expect = 1.487e-17
Identity = 11/14 (78.57%), Postives = 12/14 (85.71%), Query Frame = 3

Query:  132 ETFLFTSESVNEGH 173
            E  LFTSESV+EGH
Sbjct:    3 EYSLFTSESVSEGH 16

[Close]

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_PSEF5 (S-adenosylmethionine synthetase OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=metK PE=3 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 1.487e-17
Identity = 41/70 (58.57%), Postives = 52/70 (74.29%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+ DQ+SDAVLDA +AQD  ++VA ET  KT + +V GE+TT A VD E+IVRD    IG+TS+DVG D
Sbjct:   18 DKIADQISDAVLDAIIAQDKHARVAVETLVKTGVAIVAGEVTTSAWVDLEQIVRDVICDIGYTSSDVGFD 87

HSP 2 Score: 25.0238 bits (53), Expect = 1.487e-17
Identity = 11/14 (78.57%), Postives = 12/14 (85.71%), Query Frame = 3

Query:  132 ETFLFTSESVNEGH 173
            E  LFTSESV+EGH
Sbjct:    3 EYSLFTSESVSEGH 16

[Close]

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_CLOAB (S-adenosylmethionine synthetase OS=Clostridium acetobutylicum GN=metK PE=3 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 1.487e-17
Identity = 40/70 (57.14%), Postives = 49/70 (70.00%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+CDQ+SDA+LDA L +DP+ +VACET   T +V V GEI+T   VD  KIVR T R IG+T A  G D
Sbjct:   16 DKICDQISDAILDAILEKDPNGRVACETTVTTGIVNVMGEISTNCYVDIPKIVRKTVREIGYTRAKYGFD 85

HSP 2 Score: 24.2534 bits (51), Expect = 1.487e-17
Identity = 10/11 (90.91%), Postives = 10/11 (90.91%), Query Frame = 3

Query:  141 LFTSESVNEGH 173
            LFTSESV EGH
Sbjct:    4 LFTSESVTEGH 14

[Close]

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_PSYWF (S-adenosylmethionine synthetase OS=Psychrobacter sp. (strain PRwf-1) GN=metK PE=3 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 1.487e-17
Identity = 38/70 (54.29%), Postives = 53/70 (75.71%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+ DQ+SDA+LDA + +D  ++VACET  KT  V++ GEI+T AN+D E+IVRDT  SIG+  +D+G D
Sbjct:   18 DKMADQISDALLDAIMREDLHARVACETLVKTGAVVLAGEISTTANIDIERIVRDTVNSIGYNHSDLGFD 87

HSP 2 Score: 24.6386 bits (52), Expect = 1.487e-17
Identity = 10/11 (90.91%), Postives = 11/11 (100.00%), Query Frame = 3

Query:  141 LFTSESVNEGH 173
            LFTSESV+EGH
Sbjct:    6 LFTSESVSEGH 16

[Close]

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_GEOSL (S-adenosylmethionine synthetase OS=Geobacter sulfurreducens GN=metK PE=3 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 1.487e-17
Identity = 38/70 (54.29%), Postives = 50/70 (71.43%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+ DQVSDA+LDA L QD  ++VACET   T M ++ GEITT A VDY K+VR+T + IG+  + +G D
Sbjct:   19 DKVADQVSDAILDAILTQDKRARVACETLVTTGMAVIAGEITTTAVVDYPKVVRETIKEIGYNDSAMGFD 88

HSP 2 Score: 26.9498 bits (58), Expect = 1.487e-17
Identity = 10/15 (66.67%), Postives = 14/15 (93.33%), Query Frame = 3

Query:  129 METFLFTSESVNEGH 173
            M+ ++FTSESV+EGH
Sbjct:    3 MKDYIFTSESVSEGH 17

[Close]

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_BACCN (S-adenosylmethionine synthetase OS=Bacillus cereus subsp. cytotoxis (strain NVH 391-98) GN=metK PE=3 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 1.833e-17
Identity = 43/88 (48.86%), Postives = 61/88 (69.32%), Query Frame = 1

Query:  127 KWKHFFSHQNQ*TRVTSDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDA 390
            K +H F+ ++  T    DK+CDQ+SD++LDA L++D +++VACET   T +V+V GEITT   VD  KIVR+T + IG+T A  G DA
Sbjct:    3 KQRHLFTSESV-TEGHPDKICDQISDSILDAILSKDANARVACETTVTTGLVLVAGEITTSTYVDIPKIVRETIQGIGYTRAKYGFDA 89

[Close]

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_CHRSD (S-adenosylmethionine synthetase OS=Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768) GN=metK PE=3 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 1.932e-17
Identity = 40/70 (57.14%), Postives = 51/70 (72.86%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+ DQ+SDAVLDA +A+D  ++VACET  KT + +V GEITT A VD E +VR     IG+TS+DVG D
Sbjct:   18 DKIADQISDAVLDAIIARDKQARVACETMVKTGVAIVAGEITTSAWVDLEDLVRRVINDIGYTSSDVGFD 87

HSP 2 Score: 25.0238 bits (53), Expect = 1.932e-17
Identity = 11/14 (78.57%), Postives = 12/14 (85.71%), Query Frame = 3

Query:  132 ETFLFTSESVNEGH 173
            E  LFTSESV+EGH
Sbjct:    3 EYSLFTSESVSEGH 16

[Close]

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_XANOR (S-adenosylmethionine synthetase OS=Xanthomonas oryzae pv. oryzae GN=metK PE=3 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 1.932e-17
Identity = 38/70 (54.29%), Postives = 49/70 (70.00%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+ DQ+SDAVLDA LAQD  ++VACET  KT + +V GE+TT A +D E + R     IG+ S+DVG D
Sbjct:   17 DKIADQISDAVLDAILAQDKRARVACETMVKTGVAIVAGEVTTSAWIDLEALTRKVILDIGYNSSDVGFD 86

HSP 2 Score: 28.1054 bits (61), Expect = 1.932e-17
Identity = 11/15 (73.33%), Postives = 14/15 (93.33%), Query Frame = 3

Query:  129 METFLFTSESVNEGH 173
            M ++LFTSESV+EGH
Sbjct:    1 MSSYLFTSESVSEGH 15

[Close]

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_XANOP (S-adenosylmethionine synthetase OS=Xanthomonas oryzae pv. oryzae (strain PXO99A) GN=metK PE=3 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 1.932e-17
Identity = 38/70 (54.29%), Postives = 49/70 (70.00%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+ DQ+SDAVLDA LAQD  ++VACET  KT + +V GE+TT A +D E + R     IG+ S+DVG D
Sbjct:   17 DKIADQISDAVLDAILAQDKRARVACETMVKTGVAIVAGEVTTSAWIDLEALTRKVILDIGYNSSDVGFD 86

HSP 2 Score: 28.1054 bits (61), Expect = 1.932e-17
Identity = 11/15 (73.33%), Postives = 14/15 (93.33%), Query Frame = 3

Query:  129 METFLFTSESVNEGH 173
            M ++LFTSESV+EGH
Sbjct:    1 MSSYLFTSESVSEGH 15

[Close]

BLAST of CF504285 vs. ExPASy Swiss-Prot
Match: METK_XANOM (S-adenosylmethionine synthetase OS=Xanthomonas oryzae pv. oryzae (strain MAFF 311018) GN=metK PE=3 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 1.932e-17
Identity = 38/70 (54.29%), Postives = 49/70 (70.00%), Query Frame = 1

Query:  178 DKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLD 387
            DK+ DQ+SDAVLDA LAQD  ++VACET  KT + +V GE+TT A +D E + R     IG+ S+DVG D
Sbjct:   17 DKIADQISDAVLDAILAQDKRARVACETMVKTGVAIVAGEVTTSAWIDLEALTRKVILDIGYNSSDVGFD 86

HSP 2 Score: 28.1054 bits (61), Expect = 1.932e-17
Identity = 11/15 (73.33%), Postives = 14/15 (93.33%), Query Frame = 3

Query:  129 METFLFTSESVNEGH 173
            M ++LFTSESV+EGH
Sbjct:    1 MSSYLFTSESVSEGH 15

The following BLAST results are available for this feature:

BLAST of CF504285 vs. ExPASy Swiss-Prot

BLAST of CF504285 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
METK_PSEPF	1.487e-17	58.57	S-adenosylmethionine synthetase OS=Pseudomonas flu...	[more]
METK_PSEF5	1.487e-17	58.57	S-adenosylmethionine synthetase OS=Pseudomonas flu...	[more]
METK_CLOAB	1.487e-17	57.14	S-adenosylmethionine synthetase OS=Clostridium ace...	[more]
METK_PSYWF	1.487e-17	54.29	S-adenosylmethionine synthetase OS=Psychrobacter s...	[more]
METK_GEOSL	1.487e-17	54.29	S-adenosylmethionine synthetase OS=Geobacter sulfu...	[more]
METK_BACCN	1.833e-17	48.86	S-adenosylmethionine synthetase OS=Bacillus cereus...	[more]
METK_CHRSD	1.932e-17	57.14	S-adenosylmethionine synthetase OS=Chromohalobacte...	[more]
METK_XANOR	1.932e-17	54.29	S-adenosylmethionine synthetase OS=Xanthomonas ory...	[more]
METK_XANOP	1.932e-17	54.29	S-adenosylmethionine synthetase OS=Xanthomonas ory...	[more]
METK_XANOM	1.932e-17	54.29	S-adenosylmethionine synthetase OS=Xanthomonas ory...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_119000-774 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-16_E09 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF504285 ID=CF504285; Name=CF504285; organism=Citrus sinensis; type=EST; length=390bp
CGTCTTTCTCTCGTTCTTCTGGGTACTTACCATTTTCGTATATCACCACC
TTCAATTCCATTTGTACTCGCACCACGGCCTCCTGCAAAGCGAAAGCTGC
AGCTTCAGGTTAAATCAAATTAAAGAAAATGGAAACATTTCTTTTCACAT
CAGAATCAGTGAACGAGGGTCACCTCGGACAAGCTGTGTGACCAAGTTTC
GGACGCAGTACTGGACGCGTGTTTGGCTCAGGATCCTGACAGCAAAGTTG
CTTGTGAAACATGCACAAAGACCAACATGGTCATGGTTTTTGGAGAAATT
ACAACCAAGGCCAATGTGGATTACGAGAAAATCGTGCGTGATACATGCAG
ATCGATTGGGTTCACATCGGCCGATGTGGGGCTTGATGCT